New Method to determine Antioxidant Activity of Polyphenols

Autores
Chaillou, Lucrecia Lucia; Nazareno, Mónica Azucena
Año de publicación
2006
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The capacity of polyphenolic compounds to reduce the â-carotene-linoleic acid cooxidationenzymatically induced by soybean lipoxygenase was assayed to determine their comprehensiveantioxidant ability. The inhibition of the coupled oxidation is a well-known spectrophotometric methodfor the antioxidant activity measurement. A modification of this method is proposed to reduce assaytime and to gain simplicity. The antioxidant abilities of several polyphenols were determined, andquercetin and sinapic acid were most active. These results were compared to those obtained by theDPPH¥ procedure to evaluate the free radical scavenging contribution to the total protective action.The highest values of antiradical activity were found for ellagic and quercetin.
Fil: Chaillou, Lucrecia Lucia. Universidad Nacional de Santiago del Estero. Facultad de Agronomía y Agroindustrias. Instituto de Ciencias Químicas; Argentina
Fil: Nazareno, Mónica Azucena. Universidad Nacional de Santiago del Estero. Facultad de Agronomía y Agroindustrias. Instituto de Ciencias Químicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
PHENOLIC COMPOUNDS
ANTIRADICAL
FREE RADICALS
PROPOLIS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/244738
Acceder
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network_name_str CONICET Digital (CONICET)
spelling New Method to determine Antioxidant Activity of PolyphenolsChaillou, Lucrecia LuciaNazareno, Mónica AzucenaPHENOLIC COMPOUNDSANTIRADICALFREE RADICALSPROPOLIShttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1The capacity of polyphenolic compounds to reduce the â-carotene-linoleic acid cooxidationenzymatically induced by soybean lipoxygenase was assayed to determine their comprehensiveantioxidant ability. The inhibition of the coupled oxidation is a well-known spectrophotometric methodfor the antioxidant activity measurement. A modification of this method is proposed to reduce assaytime and to gain simplicity. The antioxidant abilities of several polyphenols were determined, andquercetin and sinapic acid were most active. These results were compared to those obtained by theDPPH¥ procedure to evaluate the free radical scavenging contribution to the total protective action.The highest values of antiradical activity were found for ellagic and quercetin.Fil: Chaillou, Lucrecia Lucia. Universidad Nacional de Santiago del Estero. Facultad de Agronomía y Agroindustrias. Instituto de Ciencias Químicas; ArgentinaFil: Nazareno, Mónica Azucena. Universidad Nacional de Santiago del Estero. Facultad de Agronomía y Agroindustrias. Instituto de Ciencias Químicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaAmerican Chemical Society2006-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/244738Chaillou, Lucrecia Lucia; Nazareno, Mónica Azucena; New Method to determine Antioxidant Activity of Polyphenols; American Chemical Society; Journal of Agricultural and Food Chemistry; 54; 22; 9-2006; 8397-84020021-8561CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://pubs.acs.org/doi/10.1021/jf061729finfo:eu-repo/semantics/altIdentifier/doi/10.1021/jf061729finfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:27:43Zoai:ri.conicet.gov.ar:11336/244738instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:27:43.435CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv New Method to determine Antioxidant Activity of Polyphenols
title New Method to determine Antioxidant Activity of Polyphenols
spellingShingle New Method to determine Antioxidant Activity of Polyphenols
Chaillou, Lucrecia Lucia
PHENOLIC COMPOUNDS
ANTIRADICAL
FREE RADICALS
PROPOLIS
title_short New Method to determine Antioxidant Activity of Polyphenols
title_full New Method to determine Antioxidant Activity of Polyphenols
title_fullStr New Method to determine Antioxidant Activity of Polyphenols
title_full_unstemmed New Method to determine Antioxidant Activity of Polyphenols
title_sort New Method to determine Antioxidant Activity of Polyphenols
dc.creator.none.fl_str_mv Chaillou, Lucrecia Lucia
Nazareno, Mónica Azucena
author Chaillou, Lucrecia Lucia
author_facet Chaillou, Lucrecia Lucia
Nazareno, Mónica Azucena
author_role author
author2 Nazareno, Mónica Azucena
author2_role author
dc.subject.none.fl_str_mv PHENOLIC COMPOUNDS
ANTIRADICAL
FREE RADICALS
PROPOLIS
topic PHENOLIC COMPOUNDS
ANTIRADICAL
FREE RADICALS
PROPOLIS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The capacity of polyphenolic compounds to reduce the â-carotene-linoleic acid cooxidationenzymatically induced by soybean lipoxygenase was assayed to determine their comprehensiveantioxidant ability. The inhibition of the coupled oxidation is a well-known spectrophotometric methodfor the antioxidant activity measurement. A modification of this method is proposed to reduce assaytime and to gain simplicity. The antioxidant abilities of several polyphenols were determined, andquercetin and sinapic acid were most active. These results were compared to those obtained by theDPPH¥ procedure to evaluate the free radical scavenging contribution to the total protective action.The highest values of antiradical activity were found for ellagic and quercetin.
Fil: Chaillou, Lucrecia Lucia. Universidad Nacional de Santiago del Estero. Facultad de Agronomía y Agroindustrias. Instituto de Ciencias Químicas; Argentina
Fil: Nazareno, Mónica Azucena. Universidad Nacional de Santiago del Estero. Facultad de Agronomía y Agroindustrias. Instituto de Ciencias Químicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description The capacity of polyphenolic compounds to reduce the â-carotene-linoleic acid cooxidationenzymatically induced by soybean lipoxygenase was assayed to determine their comprehensiveantioxidant ability. The inhibition of the coupled oxidation is a well-known spectrophotometric methodfor the antioxidant activity measurement. A modification of this method is proposed to reduce assaytime and to gain simplicity. The antioxidant abilities of several polyphenols were determined, andquercetin and sinapic acid were most active. These results were compared to those obtained by theDPPH¥ procedure to evaluate the free radical scavenging contribution to the total protective action.The highest values of antiradical activity were found for ellagic and quercetin.
publishDate 2006
dc.date.none.fl_str_mv 2006-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/244738
Chaillou, Lucrecia Lucia; Nazareno, Mónica Azucena; New Method to determine Antioxidant Activity of Polyphenols; American Chemical Society; Journal of Agricultural and Food Chemistry; 54; 22; 9-2006; 8397-8402
0021-8561
CONICET Digital
CONICET
url http://hdl.handle.net/11336/244738
identifier_str_mv Chaillou, Lucrecia Lucia; Nazareno, Mónica Azucena; New Method to determine Antioxidant Activity of Polyphenols; American Chemical Society; Journal of Agricultural and Food Chemistry; 54; 22; 9-2006; 8397-8402
0021-8561
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://pubs.acs.org/doi/10.1021/jf061729f
info:eu-repo/semantics/altIdentifier/doi/10.1021/jf061729f
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Chemical Society
publisher.none.fl_str_mv American Chemical Society
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846083418567213056
score 13.22299

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