Effect of a biological response modifier on expression of CD14 receptor and tumor necrosis factor-alpha in Staphylococcus aureus-infected mammary glands at drying off

Autores
Dallard, Bibiana Elisabet; Baravalle, Celina; Ortega, Hugo Hector; Tumini, M.; Canavesio, V.R.; Neder, V.E.; Calvinho, Luis Fernando
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Agents that increase natural protective mechanisms have been proposed for prevention and treatment of intramammary infections. The objectives of this study were to get an insight of innate immune mechanisms that occur during bovine mammary involution in both uninfected and chronically Staphylococcus aureus-infected glands and to describe the effects on those mechanisms of a single intramammary infusion of a LPS-based biological response modifier (BRM) at the end of lactation. Three groups of 12 cows, each one including 6 S. aureus-infected and 6 uninfected, were infused in two mammary quarters with BRM or placebo and sacrificed at 7, 14 and 21 d of involution. In uninfected and S. aureus-infected quarters treated with a BRM, the number of monocytes/macrophages detected with CD14 antibody was significantly higher (P < 0.05) than in placebo-treated quarters at every sampling evaluation period. In uninfected quarters, the TNF-α staining area was not affected by BRM treatment. However, in infected quarters, the immunostained area for TNF-α was significantly higher than in uninfected quarters and BRM treatment was associated with increased staining at 21 d of involution.
Fil: Dallard, Bibiana Elisabet. Universidad Nacional del Litoral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Baravalle, Celina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Litoral; Argentina
Fil: Ortega, Hugo Hector. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Litoral; Argentina
Fil: Tumini, M.. Universidad Nacional del Litoral; Argentina
Fil: Canavesio, V.R.. Instituto Nacional de Tecnología Agropecuaria Eea, Rafaela; Argentina
Fil: Neder, V.E.. Instituto Nacional de Tecnología Agropecuaria Eea, Rafaela; Argentina
Fil: Calvinho, Luis Fernando. Universidad Nacional del Litoral; Argentina. Instituto Nacional de Tecnología Agropecuaria Eea, Rafaela; Argentina
Materia
BIOLOGICAL RESPONSE MODIFIER
BOVINE MAMMARY GLAND
CD14 RECEPTOR
STAPHYLOCOCCUS AUREUS
TNF-&Alpha;
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/97602
Acceder
id CONICETDig_127de73564d57448750a9271b483dca2
oai_identifier_str oai:ri.conicet.gov.ar:11336/97602
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Effect of a biological response modifier on expression of CD14 receptor and tumor necrosis factor-alpha in Staphylococcus aureus-infected mammary glands at drying offDallard, Bibiana ElisabetBaravalle, CelinaOrtega, Hugo HectorTumini, M.Canavesio, V.R.Neder, V.E.Calvinho, Luis FernandoBIOLOGICAL RESPONSE MODIFIERBOVINE MAMMARY GLANDCD14 RECEPTORSTAPHYLOCOCCUS AUREUSTNF-&Alpha;https://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Agents that increase natural protective mechanisms have been proposed for prevention and treatment of intramammary infections. The objectives of this study were to get an insight of innate immune mechanisms that occur during bovine mammary involution in both uninfected and chronically Staphylococcus aureus-infected glands and to describe the effects on those mechanisms of a single intramammary infusion of a LPS-based biological response modifier (BRM) at the end of lactation. Three groups of 12 cows, each one including 6 S. aureus-infected and 6 uninfected, were infused in two mammary quarters with BRM or placebo and sacrificed at 7, 14 and 21 d of involution. In uninfected and S. aureus-infected quarters treated with a BRM, the number of monocytes/macrophages detected with CD14 antibody was significantly higher (P < 0.05) than in placebo-treated quarters at every sampling evaluation period. In uninfected quarters, the TNF-α staining area was not affected by BRM treatment. However, in infected quarters, the immunostained area for TNF-α was significantly higher than in uninfected quarters and BRM treatment was associated with increased staining at 21 d of involution.Fil: Dallard, Bibiana Elisabet. Universidad Nacional del Litoral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Baravalle, Celina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Litoral; ArgentinaFil: Ortega, Hugo Hector. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Litoral; ArgentinaFil: Tumini, M.. Universidad Nacional del Litoral; ArgentinaFil: Canavesio, V.R.. Instituto Nacional de Tecnología Agropecuaria Eea, Rafaela; ArgentinaFil: Neder, V.E.. Instituto Nacional de Tecnología Agropecuaria Eea, Rafaela; ArgentinaFil: Calvinho, Luis Fernando. Universidad Nacional del Litoral; Argentina. Instituto Nacional de Tecnología Agropecuaria Eea, Rafaela; ArgentinaElsevier Science2009-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/97602Dallard, Bibiana Elisabet; Baravalle, Celina; Ortega, Hugo Hector; Tumini, M.; Canavesio, V.R.; et al.; Effect of a biological response modifier on expression of CD14 receptor and tumor necrosis factor-alpha in Staphylococcus aureus-infected mammary glands at drying off; Elsevier Science; Veterinary Immunology And Immunopathology; 132; 2-4; 12-2009; 237-2420165-2427CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://sciencedirect.com/science/journal/01652427info:eu-repo/semantics/altIdentifier/doi/10.1016/j.vetimm.2009.05.004info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:54:38Zoai:ri.conicet.gov.ar:11336/97602instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:54:38.881CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effect of a biological response modifier on expression of CD14 receptor and tumor necrosis factor-alpha in Staphylococcus aureus-infected mammary glands at drying off
title Effect of a biological response modifier on expression of CD14 receptor and tumor necrosis factor-alpha in Staphylococcus aureus-infected mammary glands at drying off
spellingShingle Effect of a biological response modifier on expression of CD14 receptor and tumor necrosis factor-alpha in Staphylococcus aureus-infected mammary glands at drying off
Dallard, Bibiana Elisabet
BIOLOGICAL RESPONSE MODIFIER
BOVINE MAMMARY GLAND
CD14 RECEPTOR
STAPHYLOCOCCUS AUREUS
TNF-&Alpha;
title_short Effect of a biological response modifier on expression of CD14 receptor and tumor necrosis factor-alpha in Staphylococcus aureus-infected mammary glands at drying off
title_full Effect of a biological response modifier on expression of CD14 receptor and tumor necrosis factor-alpha in Staphylococcus aureus-infected mammary glands at drying off
title_fullStr Effect of a biological response modifier on expression of CD14 receptor and tumor necrosis factor-alpha in Staphylococcus aureus-infected mammary glands at drying off
title_full_unstemmed Effect of a biological response modifier on expression of CD14 receptor and tumor necrosis factor-alpha in Staphylococcus aureus-infected mammary glands at drying off
title_sort Effect of a biological response modifier on expression of CD14 receptor and tumor necrosis factor-alpha in Staphylococcus aureus-infected mammary glands at drying off
dc.creator.none.fl_str_mv Dallard, Bibiana Elisabet
Baravalle, Celina
Ortega, Hugo Hector
Tumini, M.
Canavesio, V.R.
Neder, V.E.
Calvinho, Luis Fernando
author Dallard, Bibiana Elisabet
author_facet Dallard, Bibiana Elisabet
Baravalle, Celina
Ortega, Hugo Hector
Tumini, M.
Canavesio, V.R.
Neder, V.E.
Calvinho, Luis Fernando
author_role author
author2 Baravalle, Celina
Ortega, Hugo Hector
Tumini, M.
Canavesio, V.R.
Neder, V.E.
Calvinho, Luis Fernando
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv BIOLOGICAL RESPONSE MODIFIER
BOVINE MAMMARY GLAND
CD14 RECEPTOR
STAPHYLOCOCCUS AUREUS
TNF-&Alpha;
topic BIOLOGICAL RESPONSE MODIFIER
BOVINE MAMMARY GLAND
CD14 RECEPTOR
STAPHYLOCOCCUS AUREUS
TNF-&Alpha;
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Agents that increase natural protective mechanisms have been proposed for prevention and treatment of intramammary infections. The objectives of this study were to get an insight of innate immune mechanisms that occur during bovine mammary involution in both uninfected and chronically Staphylococcus aureus-infected glands and to describe the effects on those mechanisms of a single intramammary infusion of a LPS-based biological response modifier (BRM) at the end of lactation. Three groups of 12 cows, each one including 6 S. aureus-infected and 6 uninfected, were infused in two mammary quarters with BRM or placebo and sacrificed at 7, 14 and 21 d of involution. In uninfected and S. aureus-infected quarters treated with a BRM, the number of monocytes/macrophages detected with CD14 antibody was significantly higher (P < 0.05) than in placebo-treated quarters at every sampling evaluation period. In uninfected quarters, the TNF-α staining area was not affected by BRM treatment. However, in infected quarters, the immunostained area for TNF-α was significantly higher than in uninfected quarters and BRM treatment was associated with increased staining at 21 d of involution.
Fil: Dallard, Bibiana Elisabet. Universidad Nacional del Litoral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Baravalle, Celina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Litoral; Argentina
Fil: Ortega, Hugo Hector. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Litoral; Argentina
Fil: Tumini, M.. Universidad Nacional del Litoral; Argentina
Fil: Canavesio, V.R.. Instituto Nacional de Tecnología Agropecuaria Eea, Rafaela; Argentina
Fil: Neder, V.E.. Instituto Nacional de Tecnología Agropecuaria Eea, Rafaela; Argentina
Fil: Calvinho, Luis Fernando. Universidad Nacional del Litoral; Argentina. Instituto Nacional de Tecnología Agropecuaria Eea, Rafaela; Argentina
description Agents that increase natural protective mechanisms have been proposed for prevention and treatment of intramammary infections. The objectives of this study were to get an insight of innate immune mechanisms that occur during bovine mammary involution in both uninfected and chronically Staphylococcus aureus-infected glands and to describe the effects on those mechanisms of a single intramammary infusion of a LPS-based biological response modifier (BRM) at the end of lactation. Three groups of 12 cows, each one including 6 S. aureus-infected and 6 uninfected, were infused in two mammary quarters with BRM or placebo and sacrificed at 7, 14 and 21 d of involution. In uninfected and S. aureus-infected quarters treated with a BRM, the number of monocytes/macrophages detected with CD14 antibody was significantly higher (P < 0.05) than in placebo-treated quarters at every sampling evaluation period. In uninfected quarters, the TNF-α staining area was not affected by BRM treatment. However, in infected quarters, the immunostained area for TNF-α was significantly higher than in uninfected quarters and BRM treatment was associated with increased staining at 21 d of involution.
publishDate 2009
dc.date.none.fl_str_mv 2009-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/97602
Dallard, Bibiana Elisabet; Baravalle, Celina; Ortega, Hugo Hector; Tumini, M.; Canavesio, V.R.; et al.; Effect of a biological response modifier on expression of CD14 receptor and tumor necrosis factor-alpha in Staphylococcus aureus-infected mammary glands at drying off; Elsevier Science; Veterinary Immunology And Immunopathology; 132; 2-4; 12-2009; 237-242
0165-2427
CONICET Digital
CONICET
url http://hdl.handle.net/11336/97602
identifier_str_mv Dallard, Bibiana Elisabet; Baravalle, Celina; Ortega, Hugo Hector; Tumini, M.; Canavesio, V.R.; et al.; Effect of a biological response modifier on expression of CD14 receptor and tumor necrosis factor-alpha in Staphylococcus aureus-infected mammary glands at drying off; Elsevier Science; Veterinary Immunology And Immunopathology; 132; 2-4; 12-2009; 237-242
0165-2427
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://sciencedirect.com/science/journal/01652427
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.vetimm.2009.05.004
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269297998561280
score 13.13397

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