Early adaptive immune responses in the respiratory tract of foot-and-mouth disease virus-infected cattle

Autores
Pega, Juan Franco; Bucafusco, Danilo; Di Giacomo, S.; Schammas, Juan Manuel; Malacari, D.; Capozzo, Alejandra Victoria; Arzt, J.; Pérez Beascoeachea, C.; Maradei, E.; Rodríguez, L. L.; Borca, Manuel Victor; Pérez Filgueira, Daniel Mariano
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Foot-and-mouth disease (FMD) is a highly contagious viral disease which affects both domestic and wild biungulate species. This acute disease, caused by the FMD virus (FMDV), usually includes an active replication phase in the respiratory tract for up to 72 h postinfection, followed by hematogenous dissemination and vesicular lesions at oral and foot epithelia. The role of the early local adaptive immunity of the host in the outcome of the infection is not well understood. Here we report the kinetics of appearance of FMDV-specific antibody-secreting cells (ASC) in lymphoid organs along the respiratory tract and the spleen in cattle infected by aerosol exposure. While no responses were observed for up to 3 days postinfection (dpi), all animals developed FMDV-ASC in all the lymphoid organs studied at 4 dpi. Tracheobronchial lymph nodes were the most reactive organs at this time, and IgM was the predominant isotype, followed by IgG1. Numbers of FMDV-ASC were further augmented at 5 and 6 dpi, with an increasing prevalence in upper respiratory organs. Systemic antibody responses were slightly delayed compared with the local reaction. Also, IgM was the dominant isotype in serum at 5 dpi, coinciding with a sharp decrease of viral RNA detection in peripheral blood. These results indicate that following aerogenous administration, cattle develop a rapid and vigorous genuine local antibody response throughout the respiratory tract. Time course and isotype profiles indicate the presence of an efficient T cell-independent antibody response which drives the IgM-mediated virus clearance in cattle infected by FMDV aerosol exposure.
Fil: Pega, Juan Franco. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Bucafusco, Danilo. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Di Giacomo, S.. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina
Fil: Schammas, Juan Manuel. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina
Fil: Malacari, D.. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina
Fil: Capozzo, Alejandra Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina
Fil: Arzt, J.. United States Department of Agriculture. Agricultural Research Service; Argentina
Fil: Pérez Beascoeachea, C.. Ministerio de Agricultura, Ganadería, Pesca y Alimento. Servicio Nacional de Sanidad y Calidad Agroalimentaria; Argentina
Fil: Maradei, E.. Ministerio de Agricultura, Ganadería, Pesca y Alimento. Servicio Nacional de Sanidad y Calidad Agroalimentaria; Argentina
Fil: Rodríguez, L. L.. United States Department of Agriculture. Agricultural Research Service; Argentina
Fil: Borca, Manuel Victor. United States Department of Agriculture. Agricultural Research Service; Argentina
Fil: Pérez Filgueira, Daniel Mariano. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
FOOT AND MOUTH DISEASE VIRUS
EARLY RESPONSES
MUCOSAL SITES
AEROSOLIZED INFECTION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/24004
Acceder
id CONICETDig_115bf140dfaeb17258a6fecb6e93f1cd
oai_identifier_str oai:ri.conicet.gov.ar:11336/24004
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Early adaptive immune responses in the respiratory tract of foot-and-mouth disease virus-infected cattlePega, Juan FrancoBucafusco, DaniloDi Giacomo, S.Schammas, Juan ManuelMalacari, D.Capozzo, Alejandra VictoriaArzt, J.Pérez Beascoeachea, C.Maradei, E.Rodríguez, L. L.Borca, Manuel VictorPérez Filgueira, Daniel MarianoFOOT AND MOUTH DISEASE VIRUSEARLY RESPONSESMUCOSAL SITESAEROSOLIZED INFECTIONhttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Foot-and-mouth disease (FMD) is a highly contagious viral disease which affects both domestic and wild biungulate species. This acute disease, caused by the FMD virus (FMDV), usually includes an active replication phase in the respiratory tract for up to 72 h postinfection, followed by hematogenous dissemination and vesicular lesions at oral and foot epithelia. The role of the early local adaptive immunity of the host in the outcome of the infection is not well understood. Here we report the kinetics of appearance of FMDV-specific antibody-secreting cells (ASC) in lymphoid organs along the respiratory tract and the spleen in cattle infected by aerosol exposure. While no responses were observed for up to 3 days postinfection (dpi), all animals developed FMDV-ASC in all the lymphoid organs studied at 4 dpi. Tracheobronchial lymph nodes were the most reactive organs at this time, and IgM was the predominant isotype, followed by IgG1. Numbers of FMDV-ASC were further augmented at 5 and 6 dpi, with an increasing prevalence in upper respiratory organs. Systemic antibody responses were slightly delayed compared with the local reaction. Also, IgM was the dominant isotype in serum at 5 dpi, coinciding with a sharp decrease of viral RNA detection in peripheral blood. These results indicate that following aerogenous administration, cattle develop a rapid and vigorous genuine local antibody response throughout the respiratory tract. Time course and isotype profiles indicate the presence of an efficient T cell-independent antibody response which drives the IgM-mediated virus clearance in cattle infected by FMDV aerosol exposure.Fil: Pega, Juan Franco. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Bucafusco, Danilo. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Di Giacomo, S.. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; ArgentinaFil: Schammas, Juan Manuel. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; ArgentinaFil: Malacari, D.. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; ArgentinaFil: Capozzo, Alejandra Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; ArgentinaFil: Arzt, J.. United States Department of Agriculture. Agricultural Research Service; ArgentinaFil: Pérez Beascoeachea, C.. Ministerio de Agricultura, Ganadería, Pesca y Alimento. Servicio Nacional de Sanidad y Calidad Agroalimentaria; ArgentinaFil: Maradei, E.. Ministerio de Agricultura, Ganadería, Pesca y Alimento. Servicio Nacional de Sanidad y Calidad Agroalimentaria; ArgentinaFil: Rodríguez, L. L.. United States Department of Agriculture. Agricultural Research Service; ArgentinaFil: Borca, Manuel Victor. United States Department of Agriculture. Agricultural Research Service; ArgentinaFil: Pérez Filgueira, Daniel Mariano. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaAmerican Society for Microbiology2013-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/24004Pega, Juan Franco; Bucafusco, Danilo; Di Giacomo, S.; Schammas, Juan Manuel; Malacari, D.; et al.; Early adaptive immune responses in the respiratory tract of foot-and-mouth disease virus-infected cattle; American Society for Microbiology; Journal of Virology; 87; 5; 3-2013; 2489-24950022-538XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://jvi.asm.org/content/87/5/2489.longinfo:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3571376/info:eu-repo/semantics/altIdentifier/doi/10.1128/JVI.02879-12info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-17T11:06:20Zoai:ri.conicet.gov.ar:11336/24004instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-17 11:06:21.251CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Early adaptive immune responses in the respiratory tract of foot-and-mouth disease virus-infected cattle
title Early adaptive immune responses in the respiratory tract of foot-and-mouth disease virus-infected cattle
spellingShingle Early adaptive immune responses in the respiratory tract of foot-and-mouth disease virus-infected cattle
Pega, Juan Franco
FOOT AND MOUTH DISEASE VIRUS
EARLY RESPONSES
MUCOSAL SITES
AEROSOLIZED INFECTION
title_short Early adaptive immune responses in the respiratory tract of foot-and-mouth disease virus-infected cattle
title_full Early adaptive immune responses in the respiratory tract of foot-and-mouth disease virus-infected cattle
title_fullStr Early adaptive immune responses in the respiratory tract of foot-and-mouth disease virus-infected cattle
title_full_unstemmed Early adaptive immune responses in the respiratory tract of foot-and-mouth disease virus-infected cattle
title_sort Early adaptive immune responses in the respiratory tract of foot-and-mouth disease virus-infected cattle
dc.creator.none.fl_str_mv Pega, Juan Franco
Bucafusco, Danilo
Di Giacomo, S.
Schammas, Juan Manuel
Malacari, D.
Capozzo, Alejandra Victoria
Arzt, J.
Pérez Beascoeachea, C.
Maradei, E.
Rodríguez, L. L.
Borca, Manuel Victor
Pérez Filgueira, Daniel Mariano
author Pega, Juan Franco
author_facet Pega, Juan Franco
Bucafusco, Danilo
Di Giacomo, S.
Schammas, Juan Manuel
Malacari, D.
Capozzo, Alejandra Victoria
Arzt, J.
Pérez Beascoeachea, C.
Maradei, E.
Rodríguez, L. L.
Borca, Manuel Victor
Pérez Filgueira, Daniel Mariano
author_role author
author2 Bucafusco, Danilo
Di Giacomo, S.
Schammas, Juan Manuel
Malacari, D.
Capozzo, Alejandra Victoria
Arzt, J.
Pérez Beascoeachea, C.
Maradei, E.
Rodríguez, L. L.
Borca, Manuel Victor
Pérez Filgueira, Daniel Mariano
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv FOOT AND MOUTH DISEASE VIRUS
EARLY RESPONSES
MUCOSAL SITES
AEROSOLIZED INFECTION
topic FOOT AND MOUTH DISEASE VIRUS
EARLY RESPONSES
MUCOSAL SITES
AEROSOLIZED INFECTION
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Foot-and-mouth disease (FMD) is a highly contagious viral disease which affects both domestic and wild biungulate species. This acute disease, caused by the FMD virus (FMDV), usually includes an active replication phase in the respiratory tract for up to 72 h postinfection, followed by hematogenous dissemination and vesicular lesions at oral and foot epithelia. The role of the early local adaptive immunity of the host in the outcome of the infection is not well understood. Here we report the kinetics of appearance of FMDV-specific antibody-secreting cells (ASC) in lymphoid organs along the respiratory tract and the spleen in cattle infected by aerosol exposure. While no responses were observed for up to 3 days postinfection (dpi), all animals developed FMDV-ASC in all the lymphoid organs studied at 4 dpi. Tracheobronchial lymph nodes were the most reactive organs at this time, and IgM was the predominant isotype, followed by IgG1. Numbers of FMDV-ASC were further augmented at 5 and 6 dpi, with an increasing prevalence in upper respiratory organs. Systemic antibody responses were slightly delayed compared with the local reaction. Also, IgM was the dominant isotype in serum at 5 dpi, coinciding with a sharp decrease of viral RNA detection in peripheral blood. These results indicate that following aerogenous administration, cattle develop a rapid and vigorous genuine local antibody response throughout the respiratory tract. Time course and isotype profiles indicate the presence of an efficient T cell-independent antibody response which drives the IgM-mediated virus clearance in cattle infected by FMDV aerosol exposure.
Fil: Pega, Juan Franco. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Bucafusco, Danilo. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Di Giacomo, S.. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina
Fil: Schammas, Juan Manuel. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina
Fil: Malacari, D.. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina
Fil: Capozzo, Alejandra Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina
Fil: Arzt, J.. United States Department of Agriculture. Agricultural Research Service; Argentina
Fil: Pérez Beascoeachea, C.. Ministerio de Agricultura, Ganadería, Pesca y Alimento. Servicio Nacional de Sanidad y Calidad Agroalimentaria; Argentina
Fil: Maradei, E.. Ministerio de Agricultura, Ganadería, Pesca y Alimento. Servicio Nacional de Sanidad y Calidad Agroalimentaria; Argentina
Fil: Rodríguez, L. L.. United States Department of Agriculture. Agricultural Research Service; Argentina
Fil: Borca, Manuel Victor. United States Department of Agriculture. Agricultural Research Service; Argentina
Fil: Pérez Filgueira, Daniel Mariano. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Foot-and-mouth disease (FMD) is a highly contagious viral disease which affects both domestic and wild biungulate species. This acute disease, caused by the FMD virus (FMDV), usually includes an active replication phase in the respiratory tract for up to 72 h postinfection, followed by hematogenous dissemination and vesicular lesions at oral and foot epithelia. The role of the early local adaptive immunity of the host in the outcome of the infection is not well understood. Here we report the kinetics of appearance of FMDV-specific antibody-secreting cells (ASC) in lymphoid organs along the respiratory tract and the spleen in cattle infected by aerosol exposure. While no responses were observed for up to 3 days postinfection (dpi), all animals developed FMDV-ASC in all the lymphoid organs studied at 4 dpi. Tracheobronchial lymph nodes were the most reactive organs at this time, and IgM was the predominant isotype, followed by IgG1. Numbers of FMDV-ASC were further augmented at 5 and 6 dpi, with an increasing prevalence in upper respiratory organs. Systemic antibody responses were slightly delayed compared with the local reaction. Also, IgM was the dominant isotype in serum at 5 dpi, coinciding with a sharp decrease of viral RNA detection in peripheral blood. These results indicate that following aerogenous administration, cattle develop a rapid and vigorous genuine local antibody response throughout the respiratory tract. Time course and isotype profiles indicate the presence of an efficient T cell-independent antibody response which drives the IgM-mediated virus clearance in cattle infected by FMDV aerosol exposure.
publishDate 2013
dc.date.none.fl_str_mv 2013-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/24004
Pega, Juan Franco; Bucafusco, Danilo; Di Giacomo, S.; Schammas, Juan Manuel; Malacari, D.; et al.; Early adaptive immune responses in the respiratory tract of foot-and-mouth disease virus-infected cattle; American Society for Microbiology; Journal of Virology; 87; 5; 3-2013; 2489-2495
0022-538X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/24004
identifier_str_mv Pega, Juan Franco; Bucafusco, Danilo; Di Giacomo, S.; Schammas, Juan Manuel; Malacari, D.; et al.; Early adaptive immune responses in the respiratory tract of foot-and-mouth disease virus-infected cattle; American Society for Microbiology; Journal of Virology; 87; 5; 3-2013; 2489-2495
0022-538X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://jvi.asm.org/content/87/5/2489.long
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3571376/
info:eu-repo/semantics/altIdentifier/doi/10.1128/JVI.02879-12
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1843606383186935808
score 13.001348

Publicaciones similares