Immobilization of beneficial vaginal lactobacilli in polymeric nanofibers for its potential inclusion in vaginal probiotic formulations

Autores
Silva, Jessica Alejandra; de Gregorio, Priscilla Romina; Rivero, Guadalupe; Abraham, Gustavo Abel; Nader, Maria Elena Fatima
Año de publicación
2019
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Lactobacilli are the predominant microorganisms in the vaginal microbiome of healthy women. Probiotic formulations containing lactic acid bacteria (LAB) must include a high number of viable and active bacteria. The aim of this work was to evaluate the compatibility, survival and maintenance of beneficial properties of Lactobacillus gasseri CRL1320 and L. rhamnosus CRL1332 during their immobilization in polymeric nanofibers by electrospinning and after storage. The compatibility of lactobacilli with mucoadhesive polymers [polyvinylalcohol (PVA), polyvinylpyrrolidone (PVP) and chitosan/polyethylene oxide (Quit/PEO)] were evaluated. Lactobacilli were electrospunned with 15 % w/v PVA (12 kV, 0.3 mL/h, 12 cm distance to aluminum collector). The membranes were later stored at room temperature, 4 and -20 °C. Lactobacillus viability, maintenance of beneficial properties (hydrophobicity, self-aggregation and antimicrobial activity against urogenital pathogens) and nanofibers characterization was performed by SEM and FITR. The combination of PVA and PVP does not affect the bacteria viability, while Quit/PEO mixture was non-compatible. Therefore, PVA was selected for LAB immobilization. Electrospinning process was efficient since it allowed the recovery of a high number of lactobacilli (1010 UFC/g nanofiber) without modifying the surface and antimicrobial properties of the two strains. Lactobacillus immobilized in nanofibers were evidenced by SEM and FTIR. A higher survival rate was obtained in L. rhamnosus CRL1332 than in L. gasseri CRL1320 after the immobilization. The highest viable cells were kept in nanofibers stored at -20 °C. However, a decrease of viable cells (lower than 1 x 107 CFU/g) was observed in L. gasseri CRL1320 and L. rhamnosus CRL1332 at 28 and 56 days, respectively. The results obtained support the inclusion of lactobacilli into polymeric nanofibers for the design of vaginal formula. However, further studies are being carried out to improve the Lactobacillus survival.
Fil: Silva, Jessica Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: de Gregorio, Priscilla Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Rivero, Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; Argentina
Fil: Abraham, Gustavo Abel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; Argentina
Fil: Nader, Maria Elena Fatima. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Reunión anual de Sociedades de Biociencia 2019; LXIV Reunión Anual de la Sociedad Argentina de Investigación Clínica; LI Reunión Anual de la Asociación Argentina de Farmacología Experimental; XXI Reunión Anual de la Sociedad Argentina de Biología; XXXI Reunión Anual de la Sociedad Argentina de Protozoología; IX Reunión Anual de la Asociación Argentina de Nanomedicinas; VI Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio
Mar del Plata
Argentina
Sociedad Argentina de Investigación Clínica
Asociación Argentina de Farmacología Experimental
Sociedad Argentina de Biología
Sociedad Argentina de Protozoología
Asociación Argentina de Nanomedicinas
Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio
Materia
MEMBRANAS NANOESTRUCTURADAS
PROBIOTICOS
ENCAPSULACIÓN
LACTOBACILLI
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/216895
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/216895
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Immobilization of beneficial vaginal lactobacilli in polymeric nanofibers for its potential inclusion in vaginal probiotic formulationsSilva, Jessica Alejandrade Gregorio, Priscilla RominaRivero, GuadalupeAbraham, Gustavo AbelNader, Maria Elena FatimaMEMBRANAS NANOESTRUCTURADASPROBIOTICOSENCAPSULACIÓNLACTOBACILLIhttps://purl.org/becyt/ford/2.9https://purl.org/becyt/ford/2Lactobacilli are the predominant microorganisms in the vaginal microbiome of healthy women. Probiotic formulations containing lactic acid bacteria (LAB) must include a high number of viable and active bacteria. The aim of this work was to evaluate the compatibility, survival and maintenance of beneficial properties of Lactobacillus gasseri CRL1320 and L. rhamnosus CRL1332 during their immobilization in polymeric nanofibers by electrospinning and after storage. The compatibility of lactobacilli with mucoadhesive polymers [polyvinylalcohol (PVA), polyvinylpyrrolidone (PVP) and chitosan/polyethylene oxide (Quit/PEO)] were evaluated. Lactobacilli were electrospunned with 15 % w/v PVA (12 kV, 0.3 mL/h, 12 cm distance to aluminum collector). The membranes were later stored at room temperature, 4 and -20 °C. Lactobacillus viability, maintenance of beneficial properties (hydrophobicity, self-aggregation and antimicrobial activity against urogenital pathogens) and nanofibers characterization was performed by SEM and FITR. The combination of PVA and PVP does not affect the bacteria viability, while Quit/PEO mixture was non-compatible. Therefore, PVA was selected for LAB immobilization. Electrospinning process was efficient since it allowed the recovery of a high number of lactobacilli (1010 UFC/g nanofiber) without modifying the surface and antimicrobial properties of the two strains. Lactobacillus immobilized in nanofibers were evidenced by SEM and FTIR. A higher survival rate was obtained in L. rhamnosus CRL1332 than in L. gasseri CRL1320 after the immobilization. The highest viable cells were kept in nanofibers stored at -20 °C. However, a decrease of viable cells (lower than 1 x 107 CFU/g) was observed in L. gasseri CRL1320 and L. rhamnosus CRL1332 at 28 and 56 days, respectively. The results obtained support the inclusion of lactobacilli into polymeric nanofibers for the design of vaginal formula. However, further studies are being carried out to improve the Lactobacillus survival.Fil: Silva, Jessica Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: de Gregorio, Priscilla Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Rivero, Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; ArgentinaFil: Abraham, Gustavo Abel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; ArgentinaFil: Nader, Maria Elena Fatima. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaReunión anual de Sociedades de Biociencia 2019; LXIV Reunión Anual de la Sociedad Argentina de Investigación Clínica; LI Reunión Anual de la Asociación Argentina de Farmacología Experimental; XXI Reunión Anual de la Sociedad Argentina de Biología; XXXI Reunión Anual de la Sociedad Argentina de Protozoología; IX Reunión Anual de la Asociación Argentina de Nanomedicinas; VI Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de LaboratorioMar del PlataArgentinaSociedad Argentina de Investigación ClínicaAsociación Argentina de Farmacología ExperimentalSociedad Argentina de BiologíaSociedad Argentina de ProtozoologíaAsociación Argentina de NanomedicinasAsociación Argentina de Ciencia y Tecnología de Animales de LaboratorioFundación Revista Medicina2019info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/216895Immobilization of beneficial vaginal lactobacilli in polymeric nanofibers for its potential inclusion in vaginal probiotic formulations; Reunión anual de Sociedades de Biociencia 2019; LXIV Reunión Anual de la Sociedad Argentina de Investigación Clínica; LI Reunión Anual de la Asociación Argentina de Farmacología Experimental; XXI Reunión Anual de la Sociedad Argentina de Biología; XXXI Reunión Anual de la Sociedad Argentina de Protozoología; IX Reunión Anual de la Asociación Argentina de Nanomedicinas; VI Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio; Mar del Plata; Argentina; 2019; 276-2770025-76801669-9106CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://aafeargentina.org/wp-content/uploads/2021/07/ACTA_2019.pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:21:08Zoai:ri.conicet.gov.ar:11336/216895instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:21:09.108CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Immobilization of beneficial vaginal lactobacilli in polymeric nanofibers for its potential inclusion in vaginal probiotic formulations
title Immobilization of beneficial vaginal lactobacilli in polymeric nanofibers for its potential inclusion in vaginal probiotic formulations
spellingShingle Immobilization of beneficial vaginal lactobacilli in polymeric nanofibers for its potential inclusion in vaginal probiotic formulations
Silva, Jessica Alejandra
MEMBRANAS NANOESTRUCTURADAS
PROBIOTICOS
ENCAPSULACIÓN
LACTOBACILLI
title_short Immobilization of beneficial vaginal lactobacilli in polymeric nanofibers for its potential inclusion in vaginal probiotic formulations
title_full Immobilization of beneficial vaginal lactobacilli in polymeric nanofibers for its potential inclusion in vaginal probiotic formulations
title_fullStr Immobilization of beneficial vaginal lactobacilli in polymeric nanofibers for its potential inclusion in vaginal probiotic formulations
title_full_unstemmed Immobilization of beneficial vaginal lactobacilli in polymeric nanofibers for its potential inclusion in vaginal probiotic formulations
title_sort Immobilization of beneficial vaginal lactobacilli in polymeric nanofibers for its potential inclusion in vaginal probiotic formulations
dc.creator.none.fl_str_mv Silva, Jessica Alejandra
de Gregorio, Priscilla Romina
Rivero, Guadalupe
Abraham, Gustavo Abel
Nader, Maria Elena Fatima
author Silva, Jessica Alejandra
author_facet Silva, Jessica Alejandra
de Gregorio, Priscilla Romina
Rivero, Guadalupe
Abraham, Gustavo Abel
Nader, Maria Elena Fatima
author_role author
author2 de Gregorio, Priscilla Romina
Rivero, Guadalupe
Abraham, Gustavo Abel
Nader, Maria Elena Fatima
author2_role author
author
author
author
dc.subject.none.fl_str_mv MEMBRANAS NANOESTRUCTURADAS
PROBIOTICOS
ENCAPSULACIÓN
LACTOBACILLI
topic MEMBRANAS NANOESTRUCTURADAS
PROBIOTICOS
ENCAPSULACIÓN
LACTOBACILLI
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.9
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Lactobacilli are the predominant microorganisms in the vaginal microbiome of healthy women. Probiotic formulations containing lactic acid bacteria (LAB) must include a high number of viable and active bacteria. The aim of this work was to evaluate the compatibility, survival and maintenance of beneficial properties of Lactobacillus gasseri CRL1320 and L. rhamnosus CRL1332 during their immobilization in polymeric nanofibers by electrospinning and after storage. The compatibility of lactobacilli with mucoadhesive polymers [polyvinylalcohol (PVA), polyvinylpyrrolidone (PVP) and chitosan/polyethylene oxide (Quit/PEO)] were evaluated. Lactobacilli were electrospunned with 15 % w/v PVA (12 kV, 0.3 mL/h, 12 cm distance to aluminum collector). The membranes were later stored at room temperature, 4 and -20 °C. Lactobacillus viability, maintenance of beneficial properties (hydrophobicity, self-aggregation and antimicrobial activity against urogenital pathogens) and nanofibers characterization was performed by SEM and FITR. The combination of PVA and PVP does not affect the bacteria viability, while Quit/PEO mixture was non-compatible. Therefore, PVA was selected for LAB immobilization. Electrospinning process was efficient since it allowed the recovery of a high number of lactobacilli (1010 UFC/g nanofiber) without modifying the surface and antimicrobial properties of the two strains. Lactobacillus immobilized in nanofibers were evidenced by SEM and FTIR. A higher survival rate was obtained in L. rhamnosus CRL1332 than in L. gasseri CRL1320 after the immobilization. The highest viable cells were kept in nanofibers stored at -20 °C. However, a decrease of viable cells (lower than 1 x 107 CFU/g) was observed in L. gasseri CRL1320 and L. rhamnosus CRL1332 at 28 and 56 days, respectively. The results obtained support the inclusion of lactobacilli into polymeric nanofibers for the design of vaginal formula. However, further studies are being carried out to improve the Lactobacillus survival.
Fil: Silva, Jessica Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: de Gregorio, Priscilla Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Rivero, Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; Argentina
Fil: Abraham, Gustavo Abel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; Argentina
Fil: Nader, Maria Elena Fatima. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Reunión anual de Sociedades de Biociencia 2019; LXIV Reunión Anual de la Sociedad Argentina de Investigación Clínica; LI Reunión Anual de la Asociación Argentina de Farmacología Experimental; XXI Reunión Anual de la Sociedad Argentina de Biología; XXXI Reunión Anual de la Sociedad Argentina de Protozoología; IX Reunión Anual de la Asociación Argentina de Nanomedicinas; VI Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio
Mar del Plata
Argentina
Sociedad Argentina de Investigación Clínica
Asociación Argentina de Farmacología Experimental
Sociedad Argentina de Biología
Sociedad Argentina de Protozoología
Asociación Argentina de Nanomedicinas
Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio
description Lactobacilli are the predominant microorganisms in the vaginal microbiome of healthy women. Probiotic formulations containing lactic acid bacteria (LAB) must include a high number of viable and active bacteria. The aim of this work was to evaluate the compatibility, survival and maintenance of beneficial properties of Lactobacillus gasseri CRL1320 and L. rhamnosus CRL1332 during their immobilization in polymeric nanofibers by electrospinning and after storage. The compatibility of lactobacilli with mucoadhesive polymers [polyvinylalcohol (PVA), polyvinylpyrrolidone (PVP) and chitosan/polyethylene oxide (Quit/PEO)] were evaluated. Lactobacilli were electrospunned with 15 % w/v PVA (12 kV, 0.3 mL/h, 12 cm distance to aluminum collector). The membranes were later stored at room temperature, 4 and -20 °C. Lactobacillus viability, maintenance of beneficial properties (hydrophobicity, self-aggregation and antimicrobial activity against urogenital pathogens) and nanofibers characterization was performed by SEM and FITR. The combination of PVA and PVP does not affect the bacteria viability, while Quit/PEO mixture was non-compatible. Therefore, PVA was selected for LAB immobilization. Electrospinning process was efficient since it allowed the recovery of a high number of lactobacilli (1010 UFC/g nanofiber) without modifying the surface and antimicrobial properties of the two strains. Lactobacillus immobilized in nanofibers were evidenced by SEM and FTIR. A higher survival rate was obtained in L. rhamnosus CRL1332 than in L. gasseri CRL1320 after the immobilization. The highest viable cells were kept in nanofibers stored at -20 °C. However, a decrease of viable cells (lower than 1 x 107 CFU/g) was observed in L. gasseri CRL1320 and L. rhamnosus CRL1332 at 28 and 56 days, respectively. The results obtained support the inclusion of lactobacilli into polymeric nanofibers for the design of vaginal formula. However, further studies are being carried out to improve the Lactobacillus survival.
publishDate 2019
dc.date.none.fl_str_mv 2019
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
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http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/216895
Immobilization of beneficial vaginal lactobacilli in polymeric nanofibers for its potential inclusion in vaginal probiotic formulations; Reunión anual de Sociedades de Biociencia 2019; LXIV Reunión Anual de la Sociedad Argentina de Investigación Clínica; LI Reunión Anual de la Asociación Argentina de Farmacología Experimental; XXI Reunión Anual de la Sociedad Argentina de Biología; XXXI Reunión Anual de la Sociedad Argentina de Protozoología; IX Reunión Anual de la Asociación Argentina de Nanomedicinas; VI Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio; Mar del Plata; Argentina; 2019; 276-277
0025-7680
1669-9106
CONICET Digital
CONICET
url http://hdl.handle.net/11336/216895
identifier_str_mv Immobilization of beneficial vaginal lactobacilli in polymeric nanofibers for its potential inclusion in vaginal probiotic formulations; Reunión anual de Sociedades de Biociencia 2019; LXIV Reunión Anual de la Sociedad Argentina de Investigación Clínica; LI Reunión Anual de la Asociación Argentina de Farmacología Experimental; XXI Reunión Anual de la Sociedad Argentina de Biología; XXXI Reunión Anual de la Sociedad Argentina de Protozoología; IX Reunión Anual de la Asociación Argentina de Nanomedicinas; VI Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio; Mar del Plata; Argentina; 2019; 276-277
0025-7680
1669-9106
CONICET Digital
CONICET
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language eng
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dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Fundación Revista Medicina
publisher.none.fl_str_mv Fundación Revista Medicina
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
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reponame_str CONICET Digital (CONICET)
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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