Viability of vaginal probiotic lactobacilli during refrigerated and frozen storage

Autores
Juárez Tomás, María Silvina; Ocaña, Virginia Soledad; Nader, Maria Elena Fatima
Año de publicación
2004
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The viability of six different strains of probiotic vaginal Lactobacillus was examined in two different cryoprotective media, during refrigerated versus frozen storage, and using two traditional types of stock cultures for starting the biomass production. Freezing at -20°C and -70°C had much less adverse effect on viability than did storage at 7°C, and the reduction in viability was greater at -20°C than at -70°C. The strains showed variation in the extent of the viability losses during both types of storage. Milk-yeast extract (MYE) was shown to be the more suitable protective medium to maintain viability of the strains during the storage. The vaginal Lactobacillus strains are most stable in MYE at -70°C with only a small decrease of the viability observed under these conditions. The viable cell counts of Lactobacillus paracasei CRL 1251 and CRL 1289, L. crispatus CRL 1266 and L. salivarius CRL 1328 remained around 1×108CFU/mL after 24 months of storage at -70°C, or up to 18 months for L. acidophilus CRL 1259.
Fil: Juárez Tomás, María Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Ocaña, Virginia Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Nader, Maria Elena Fatima. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Materia
Frozen And Refrigerated Storage
Vaginal Lactobacilli
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/56506
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Viability of vaginal probiotic lactobacilli during refrigerated and frozen storageJuárez Tomás, María SilvinaOcaña, Virginia SoledadNader, Maria Elena FatimaFrozen And Refrigerated StorageVaginal Lactobacillihttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The viability of six different strains of probiotic vaginal Lactobacillus was examined in two different cryoprotective media, during refrigerated versus frozen storage, and using two traditional types of stock cultures for starting the biomass production. Freezing at -20°C and -70°C had much less adverse effect on viability than did storage at 7°C, and the reduction in viability was greater at -20°C than at -70°C. The strains showed variation in the extent of the viability losses during both types of storage. Milk-yeast extract (MYE) was shown to be the more suitable protective medium to maintain viability of the strains during the storage. The vaginal Lactobacillus strains are most stable in MYE at -70°C with only a small decrease of the viability observed under these conditions. The viable cell counts of Lactobacillus paracasei CRL 1251 and CRL 1289, L. crispatus CRL 1266 and L. salivarius CRL 1328 remained around 1×108CFU/mL after 24 months of storage at -70°C, or up to 18 months for L. acidophilus CRL 1259.Fil: Juárez Tomás, María Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Ocaña, Virginia Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Nader, Maria Elena Fatima. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaElsevier2004-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/56506Juárez Tomás, María Silvina; Ocaña, Virginia Soledad; Nader, Maria Elena Fatima; Viability of vaginal probiotic lactobacilli during refrigerated and frozen storage; Elsevier; Anaerobe; 10; 1; 2-2004; 1-51075-99641095-8274CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.anaerobe.2004.01.002info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-29T12:34:11Zoai:ri.conicet.gov.ar:11336/56506instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-29 12:34:11.689CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Viability of vaginal probiotic lactobacilli during refrigerated and frozen storage
title Viability of vaginal probiotic lactobacilli during refrigerated and frozen storage
spellingShingle Viability of vaginal probiotic lactobacilli during refrigerated and frozen storage
Juárez Tomás, María Silvina
Frozen And Refrigerated Storage
Vaginal Lactobacilli
title_short Viability of vaginal probiotic lactobacilli during refrigerated and frozen storage
title_full Viability of vaginal probiotic lactobacilli during refrigerated and frozen storage
title_fullStr Viability of vaginal probiotic lactobacilli during refrigerated and frozen storage
title_full_unstemmed Viability of vaginal probiotic lactobacilli during refrigerated and frozen storage
title_sort Viability of vaginal probiotic lactobacilli during refrigerated and frozen storage
dc.creator.none.fl_str_mv Juárez Tomás, María Silvina
Ocaña, Virginia Soledad
Nader, Maria Elena Fatima
author Juárez Tomás, María Silvina
author_facet Juárez Tomás, María Silvina
Ocaña, Virginia Soledad
Nader, Maria Elena Fatima
author_role author
author2 Ocaña, Virginia Soledad
Nader, Maria Elena Fatima
author2_role author
author
dc.subject.none.fl_str_mv Frozen And Refrigerated Storage
Vaginal Lactobacilli
topic Frozen And Refrigerated Storage
Vaginal Lactobacilli
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The viability of six different strains of probiotic vaginal Lactobacillus was examined in two different cryoprotective media, during refrigerated versus frozen storage, and using two traditional types of stock cultures for starting the biomass production. Freezing at -20°C and -70°C had much less adverse effect on viability than did storage at 7°C, and the reduction in viability was greater at -20°C than at -70°C. The strains showed variation in the extent of the viability losses during both types of storage. Milk-yeast extract (MYE) was shown to be the more suitable protective medium to maintain viability of the strains during the storage. The vaginal Lactobacillus strains are most stable in MYE at -70°C with only a small decrease of the viability observed under these conditions. The viable cell counts of Lactobacillus paracasei CRL 1251 and CRL 1289, L. crispatus CRL 1266 and L. salivarius CRL 1328 remained around 1×108CFU/mL after 24 months of storage at -70°C, or up to 18 months for L. acidophilus CRL 1259.
Fil: Juárez Tomás, María Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Ocaña, Virginia Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Nader, Maria Elena Fatima. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
description The viability of six different strains of probiotic vaginal Lactobacillus was examined in two different cryoprotective media, during refrigerated versus frozen storage, and using two traditional types of stock cultures for starting the biomass production. Freezing at -20°C and -70°C had much less adverse effect on viability than did storage at 7°C, and the reduction in viability was greater at -20°C than at -70°C. The strains showed variation in the extent of the viability losses during both types of storage. Milk-yeast extract (MYE) was shown to be the more suitable protective medium to maintain viability of the strains during the storage. The vaginal Lactobacillus strains are most stable in MYE at -70°C with only a small decrease of the viability observed under these conditions. The viable cell counts of Lactobacillus paracasei CRL 1251 and CRL 1289, L. crispatus CRL 1266 and L. salivarius CRL 1328 remained around 1×108CFU/mL after 24 months of storage at -70°C, or up to 18 months for L. acidophilus CRL 1259.
publishDate 2004
dc.date.none.fl_str_mv 2004-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/56506
Juárez Tomás, María Silvina; Ocaña, Virginia Soledad; Nader, Maria Elena Fatima; Viability of vaginal probiotic lactobacilli during refrigerated and frozen storage; Elsevier; Anaerobe; 10; 1; 2-2004; 1-5
1075-9964
1095-8274
CONICET Digital
CONICET
url http://hdl.handle.net/11336/56506
identifier_str_mv Juárez Tomás, María Silvina; Ocaña, Virginia Soledad; Nader, Maria Elena Fatima; Viability of vaginal probiotic lactobacilli during refrigerated and frozen storage; Elsevier; Anaerobe; 10; 1; 2-2004; 1-5
1075-9964
1095-8274
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.anaerobe.2004.01.002
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.10058

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