An organic-solvent-tolerant esterase from thermophilic Bacillus licheniformis S-86

Autores
Torres, Sebastián; Martinez, Maria Alejandra; Pandey, Ashok; Castro, Guillermo Raul
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A thermophile, halotolerant and organic-solvent-tolerant esterase producer Bacillus sp. S-86 strain previously isolated was found to belong to Bacillus licheniformis species through morphological, biochemical, 16S rRNA gene sequence analyses and rDNA intergenic spacers amplification (ITS-PCR). The strain can grow at 55 °C in presence of C2–C7 alkanols (log P = −0.86 to 2.39), and NaCl concentrations up to 15% (w/v). This bacterium showed optimal growth and esterase production at 50 °C. Two different molecular weight esterase activities were detected in zymographic assays. PMSF inhibited type I esterase activity, showing no inhibitory effect on type II esterase activity. B. licheniformis S-86 was able to grow in presence of hydroxylic organic-solvents like propan-2-ol, butan-1-ol and 3-methylbutan-1-ol. At a sub-lethal concentration of these solvents (392 mmol l−1 propan-2-ol; 99 mmol l−1 butan-1-ol, 37 mmol l−1 3-methylbutan-1-ol), adequate to produce 50% cell growth inhibition at 50 °C, an increment between 1.9 and 2.3 times was observed in type I esterase production, and between 2.2 and 3.1 times in type II esterase production.
Fil: Torres, Sebastián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Martinez, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Pandey, Ashok. National Institute for Interdisciplinary Science and Technology; India
Fil: Castro, Guillermo Raul. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina. Tufts University; Estados Unidos
Materia
Esterases
B. Licheniformis
Stress
Solvent Tolerance
Non-Aqueous Biocatalysis
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/41655
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/41655
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling An organic-solvent-tolerant esterase from thermophilic Bacillus licheniformis S-86Torres, SebastiánMartinez, Maria AlejandraPandey, AshokCastro, Guillermo RaulEsterasesB. LicheniformisStressSolvent ToleranceNon-Aqueous Biocatalysishttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1A thermophile, halotolerant and organic-solvent-tolerant esterase producer Bacillus sp. S-86 strain previously isolated was found to belong to Bacillus licheniformis species through morphological, biochemical, 16S rRNA gene sequence analyses and rDNA intergenic spacers amplification (ITS-PCR). The strain can grow at 55 °C in presence of C2–C7 alkanols (log P = −0.86 to 2.39), and NaCl concentrations up to 15% (w/v). This bacterium showed optimal growth and esterase production at 50 °C. Two different molecular weight esterase activities were detected in zymographic assays. PMSF inhibited type I esterase activity, showing no inhibitory effect on type II esterase activity. B. licheniformis S-86 was able to grow in presence of hydroxylic organic-solvents like propan-2-ol, butan-1-ol and 3-methylbutan-1-ol. At a sub-lethal concentration of these solvents (392 mmol l−1 propan-2-ol; 99 mmol l−1 butan-1-ol, 37 mmol l−1 3-methylbutan-1-ol), adequate to produce 50% cell growth inhibition at 50 °C, an increment between 1.9 and 2.3 times was observed in type I esterase production, and between 2.2 and 3.1 times in type II esterase production.Fil: Torres, Sebastián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Martinez, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Pandey, Ashok. National Institute for Interdisciplinary Science and Technology; IndiaFil: Castro, Guillermo Raul. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina. Tufts University; Estados UnidosElsevier2009-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/41655Torres, Sebastián; Martinez, Maria Alejandra; Pandey, Ashok; Castro, Guillermo Raul; An organic-solvent-tolerant esterase from thermophilic Bacillus licheniformis S-86; Elsevier; Bioresource Technology; 100; 2; 1-2009; 896-9020960-8524CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.biortech.2008.07.009info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S096085240800607Xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-11-12T09:56:21Zoai:ri.conicet.gov.ar:11336/41655instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-11-12 09:56:21.273CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv An organic-solvent-tolerant esterase from thermophilic Bacillus licheniformis S-86
title An organic-solvent-tolerant esterase from thermophilic Bacillus licheniformis S-86
spellingShingle An organic-solvent-tolerant esterase from thermophilic Bacillus licheniformis S-86
Torres, Sebastián
Esterases
B. Licheniformis
Stress
Solvent Tolerance
Non-Aqueous Biocatalysis
title_short An organic-solvent-tolerant esterase from thermophilic Bacillus licheniformis S-86
title_full An organic-solvent-tolerant esterase from thermophilic Bacillus licheniformis S-86
title_fullStr An organic-solvent-tolerant esterase from thermophilic Bacillus licheniformis S-86
title_full_unstemmed An organic-solvent-tolerant esterase from thermophilic Bacillus licheniformis S-86
title_sort An organic-solvent-tolerant esterase from thermophilic Bacillus licheniformis S-86
dc.creator.none.fl_str_mv Torres, Sebastián
Martinez, Maria Alejandra
Pandey, Ashok
Castro, Guillermo Raul
author Torres, Sebastián
author_facet Torres, Sebastián
Martinez, Maria Alejandra
Pandey, Ashok
Castro, Guillermo Raul
author_role author
author2 Martinez, Maria Alejandra
Pandey, Ashok
Castro, Guillermo Raul
author2_role author
author
author
dc.subject.none.fl_str_mv Esterases
B. Licheniformis
Stress
Solvent Tolerance
Non-Aqueous Biocatalysis
topic Esterases
B. Licheniformis
Stress
Solvent Tolerance
Non-Aqueous Biocatalysis
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv A thermophile, halotolerant and organic-solvent-tolerant esterase producer Bacillus sp. S-86 strain previously isolated was found to belong to Bacillus licheniformis species through morphological, biochemical, 16S rRNA gene sequence analyses and rDNA intergenic spacers amplification (ITS-PCR). The strain can grow at 55 °C in presence of C2–C7 alkanols (log P = −0.86 to 2.39), and NaCl concentrations up to 15% (w/v). This bacterium showed optimal growth and esterase production at 50 °C. Two different molecular weight esterase activities were detected in zymographic assays. PMSF inhibited type I esterase activity, showing no inhibitory effect on type II esterase activity. B. licheniformis S-86 was able to grow in presence of hydroxylic organic-solvents like propan-2-ol, butan-1-ol and 3-methylbutan-1-ol. At a sub-lethal concentration of these solvents (392 mmol l−1 propan-2-ol; 99 mmol l−1 butan-1-ol, 37 mmol l−1 3-methylbutan-1-ol), adequate to produce 50% cell growth inhibition at 50 °C, an increment between 1.9 and 2.3 times was observed in type I esterase production, and between 2.2 and 3.1 times in type II esterase production.
Fil: Torres, Sebastián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Martinez, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Pandey, Ashok. National Institute for Interdisciplinary Science and Technology; India
Fil: Castro, Guillermo Raul. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina. Tufts University; Estados Unidos
description A thermophile, halotolerant and organic-solvent-tolerant esterase producer Bacillus sp. S-86 strain previously isolated was found to belong to Bacillus licheniformis species through morphological, biochemical, 16S rRNA gene sequence analyses and rDNA intergenic spacers amplification (ITS-PCR). The strain can grow at 55 °C in presence of C2–C7 alkanols (log P = −0.86 to 2.39), and NaCl concentrations up to 15% (w/v). This bacterium showed optimal growth and esterase production at 50 °C. Two different molecular weight esterase activities were detected in zymographic assays. PMSF inhibited type I esterase activity, showing no inhibitory effect on type II esterase activity. B. licheniformis S-86 was able to grow in presence of hydroxylic organic-solvents like propan-2-ol, butan-1-ol and 3-methylbutan-1-ol. At a sub-lethal concentration of these solvents (392 mmol l−1 propan-2-ol; 99 mmol l−1 butan-1-ol, 37 mmol l−1 3-methylbutan-1-ol), adequate to produce 50% cell growth inhibition at 50 °C, an increment between 1.9 and 2.3 times was observed in type I esterase production, and between 2.2 and 3.1 times in type II esterase production.
publishDate 2009
dc.date.none.fl_str_mv 2009-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/41655
Torres, Sebastián; Martinez, Maria Alejandra; Pandey, Ashok; Castro, Guillermo Raul; An organic-solvent-tolerant esterase from thermophilic Bacillus licheniformis S-86; Elsevier; Bioresource Technology; 100; 2; 1-2009; 896-902
0960-8524
CONICET Digital
CONICET
url http://hdl.handle.net/11336/41655
identifier_str_mv Torres, Sebastián; Martinez, Maria Alejandra; Pandey, Ashok; Castro, Guillermo Raul; An organic-solvent-tolerant esterase from thermophilic Bacillus licheniformis S-86; Elsevier; Bioresource Technology; 100; 2; 1-2009; 896-902
0960-8524
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.biortech.2008.07.009
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S096085240800607X
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.6313505

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