Sucrose synthase determines carbon allocation in developing wood and alters carbon flow at the whole tree level in aspen

Autores
Dominguez, Pia Guadalupe; Donev, Evgeniy; Derba Maceluch, Marta; Bünder, Anne; Hedenström, Mattias; Tomásková, Ivana; Mellerowicz, Ewa J.; Niittylä, Totte
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Despite the ecological and industrial importance of biomass accumulation in wood, the control of carbon (C) allocation to this tissue and to other tree tissues remain poorly understood. We studied sucrose synthase (SUS) to clarify its role in biomass formation and C metabolism at the whole tree level in hybrid aspen (Populus tremula × tremuloides). To this end, we analysed source leaves, phloem, developing wood, and roots of SUSRNAi trees using a combination of metabolite profiling, 13CO2 pulse labelling experiments, and long-term field experiments. The glasshouse grown SUSRNAi trees exhibited a mild stem phenotype together with a reduction in wood total C. The 13CO2 pulse labelling experiments showed an alteration in the C flow in all the analysed tissues, indicating that SUS affects C metabolism at the whole tree level. This was confirmed when the SUSRNAi trees were grown in the field over a 5-yr period; their stem height, diameter and biomass were substantially reduced. These results establish that SUS influences C allocation to developing wood, and that it affects C metabolism at the whole tree level.
Fil: Dominguez, Pia Guadalupe. Swedish University of Agricultural Sciences; Suecia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario; Argentina
Fil: Donev, Evgeniy. Swedish University of Agricultural Sciences; Suecia
Fil: Derba Maceluch, Marta. Swedish University of Agricultural Sciences; Suecia
Fil: Bünder, Anne. Swedish University of Agricultural Sciences; Suecia
Fil: Hedenström, Mattias. Universidad de Umea; Suecia
Fil: Tomásková, Ivana. Czech University Of Life Sciences; República Checa
Fil: Mellerowicz, Ewa J.. Swedish University of Agricultural Sciences; Suecia
Fil: Niittylä, Totte. Swedish University of Agricultural Sciences; Suecia
Materia
13C LABELLING
ASPEN
BIOMASS
CARBON ALLOCATION
POPULUS
SUCROSE SYNTHASE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/112656
Acceder
id CONICETDig_104d78cb29a094436f8fe68d883bec2e
oai_identifier_str oai:ri.conicet.gov.ar:11336/112656
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Sucrose synthase determines carbon allocation in developing wood and alters carbon flow at the whole tree level in aspenDominguez, Pia GuadalupeDonev, EvgeniyDerba Maceluch, MartaBünder, AnneHedenström, MattiasTomásková, IvanaMellerowicz, Ewa J.Niittylä, Totte13C LABELLINGASPENBIOMASSCARBON ALLOCATIONPOPULUSSUCROSE SYNTHASEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Despite the ecological and industrial importance of biomass accumulation in wood, the control of carbon (C) allocation to this tissue and to other tree tissues remain poorly understood. We studied sucrose synthase (SUS) to clarify its role in biomass formation and C metabolism at the whole tree level in hybrid aspen (Populus tremula × tremuloides). To this end, we analysed source leaves, phloem, developing wood, and roots of SUSRNAi trees using a combination of metabolite profiling, 13CO2 pulse labelling experiments, and long-term field experiments. The glasshouse grown SUSRNAi trees exhibited a mild stem phenotype together with a reduction in wood total C. The 13CO2 pulse labelling experiments showed an alteration in the C flow in all the analysed tissues, indicating that SUS affects C metabolism at the whole tree level. This was confirmed when the SUSRNAi trees were grown in the field over a 5-yr period; their stem height, diameter and biomass were substantially reduced. These results establish that SUS influences C allocation to developing wood, and that it affects C metabolism at the whole tree level.Fil: Dominguez, Pia Guadalupe. Swedish University of Agricultural Sciences; Suecia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario; ArgentinaFil: Donev, Evgeniy. Swedish University of Agricultural Sciences; SueciaFil: Derba Maceluch, Marta. Swedish University of Agricultural Sciences; SueciaFil: Bünder, Anne. Swedish University of Agricultural Sciences; SueciaFil: Hedenström, Mattias. Universidad de Umea; SueciaFil: Tomásková, Ivana. Czech University Of Life Sciences; República ChecaFil: Mellerowicz, Ewa J.. Swedish University of Agricultural Sciences; SueciaFil: Niittylä, Totte. Swedish University of Agricultural Sciences; SueciaWiley Blackwell Publishing, Inc2020-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/112656Dominguez, Pia Guadalupe; Donev, Evgeniy; Derba Maceluch, Marta; Bünder, Anne; Hedenström, Mattias; et al.; Sucrose synthase determines carbon allocation in developing wood and alters carbon flow at the whole tree level in aspen; Wiley Blackwell Publishing, Inc; New Phytologist; 6-2020; 1-130028-646XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/nph.16721info:eu-repo/semantics/altIdentifier/doi/10.1111/nph.16721info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:14:17Zoai:ri.conicet.gov.ar:11336/112656instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:14:17.413CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Sucrose synthase determines carbon allocation in developing wood and alters carbon flow at the whole tree level in aspen
title Sucrose synthase determines carbon allocation in developing wood and alters carbon flow at the whole tree level in aspen
spellingShingle Sucrose synthase determines carbon allocation in developing wood and alters carbon flow at the whole tree level in aspen
Dominguez, Pia Guadalupe
13C LABELLING
ASPEN
BIOMASS
CARBON ALLOCATION
POPULUS
SUCROSE SYNTHASE
title_short Sucrose synthase determines carbon allocation in developing wood and alters carbon flow at the whole tree level in aspen
title_full Sucrose synthase determines carbon allocation in developing wood and alters carbon flow at the whole tree level in aspen
title_fullStr Sucrose synthase determines carbon allocation in developing wood and alters carbon flow at the whole tree level in aspen
title_full_unstemmed Sucrose synthase determines carbon allocation in developing wood and alters carbon flow at the whole tree level in aspen
title_sort Sucrose synthase determines carbon allocation in developing wood and alters carbon flow at the whole tree level in aspen
dc.creator.none.fl_str_mv Dominguez, Pia Guadalupe
Donev, Evgeniy
Derba Maceluch, Marta
Bünder, Anne
Hedenström, Mattias
Tomásková, Ivana
Mellerowicz, Ewa J.
Niittylä, Totte
author Dominguez, Pia Guadalupe
author_facet Dominguez, Pia Guadalupe
Donev, Evgeniy
Derba Maceluch, Marta
Bünder, Anne
Hedenström, Mattias
Tomásková, Ivana
Mellerowicz, Ewa J.
Niittylä, Totte
author_role author
author2 Donev, Evgeniy
Derba Maceluch, Marta
Bünder, Anne
Hedenström, Mattias
Tomásková, Ivana
Mellerowicz, Ewa J.
Niittylä, Totte
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv 13C LABELLING
ASPEN
BIOMASS
CARBON ALLOCATION
POPULUS
SUCROSE SYNTHASE
topic 13C LABELLING
ASPEN
BIOMASS
CARBON ALLOCATION
POPULUS
SUCROSE SYNTHASE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Despite the ecological and industrial importance of biomass accumulation in wood, the control of carbon (C) allocation to this tissue and to other tree tissues remain poorly understood. We studied sucrose synthase (SUS) to clarify its role in biomass formation and C metabolism at the whole tree level in hybrid aspen (Populus tremula × tremuloides). To this end, we analysed source leaves, phloem, developing wood, and roots of SUSRNAi trees using a combination of metabolite profiling, 13CO2 pulse labelling experiments, and long-term field experiments. The glasshouse grown SUSRNAi trees exhibited a mild stem phenotype together with a reduction in wood total C. The 13CO2 pulse labelling experiments showed an alteration in the C flow in all the analysed tissues, indicating that SUS affects C metabolism at the whole tree level. This was confirmed when the SUSRNAi trees were grown in the field over a 5-yr period; their stem height, diameter and biomass were substantially reduced. These results establish that SUS influences C allocation to developing wood, and that it affects C metabolism at the whole tree level.
Fil: Dominguez, Pia Guadalupe. Swedish University of Agricultural Sciences; Suecia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario; Argentina
Fil: Donev, Evgeniy. Swedish University of Agricultural Sciences; Suecia
Fil: Derba Maceluch, Marta. Swedish University of Agricultural Sciences; Suecia
Fil: Bünder, Anne. Swedish University of Agricultural Sciences; Suecia
Fil: Hedenström, Mattias. Universidad de Umea; Suecia
Fil: Tomásková, Ivana. Czech University Of Life Sciences; República Checa
Fil: Mellerowicz, Ewa J.. Swedish University of Agricultural Sciences; Suecia
Fil: Niittylä, Totte. Swedish University of Agricultural Sciences; Suecia
description Despite the ecological and industrial importance of biomass accumulation in wood, the control of carbon (C) allocation to this tissue and to other tree tissues remain poorly understood. We studied sucrose synthase (SUS) to clarify its role in biomass formation and C metabolism at the whole tree level in hybrid aspen (Populus tremula × tremuloides). To this end, we analysed source leaves, phloem, developing wood, and roots of SUSRNAi trees using a combination of metabolite profiling, 13CO2 pulse labelling experiments, and long-term field experiments. The glasshouse grown SUSRNAi trees exhibited a mild stem phenotype together with a reduction in wood total C. The 13CO2 pulse labelling experiments showed an alteration in the C flow in all the analysed tissues, indicating that SUS affects C metabolism at the whole tree level. This was confirmed when the SUSRNAi trees were grown in the field over a 5-yr period; their stem height, diameter and biomass were substantially reduced. These results establish that SUS influences C allocation to developing wood, and that it affects C metabolism at the whole tree level.
publishDate 2020
dc.date.none.fl_str_mv 2020-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/112656
Dominguez, Pia Guadalupe; Donev, Evgeniy; Derba Maceluch, Marta; Bünder, Anne; Hedenström, Mattias; et al.; Sucrose synthase determines carbon allocation in developing wood and alters carbon flow at the whole tree level in aspen; Wiley Blackwell Publishing, Inc; New Phytologist; 6-2020; 1-13
0028-646X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/112656
identifier_str_mv Dominguez, Pia Guadalupe; Donev, Evgeniy; Derba Maceluch, Marta; Bünder, Anne; Hedenström, Mattias; et al.; Sucrose synthase determines carbon allocation in developing wood and alters carbon flow at the whole tree level in aspen; Wiley Blackwell Publishing, Inc; New Phytologist; 6-2020; 1-13
0028-646X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/nph.16721
info:eu-repo/semantics/altIdentifier/doi/10.1111/nph.16721
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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