Systematic analysis of decadactylar subungueal genetic traces in murder cases

Autores
Caputo, Mariela; Canonaco, Enzo; Corach, Daniel
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Foreign biological material under the fingernails of a murder victim and/or suspect can be valuable evidence in a criminal investigation. The aim of this study was to evaluate the prevalence of foreign DNA under each fingernail of victims of violent deaths, including fatal struggle or rape cases. Fingernail swabs or, alternatively clipping nails, were taken from all fingers of both right and left hands from 46 victims (13 females and 33 males) and treated separately. Semi-automated DNA extraction was performed and 21 markers were genotyped. We detected reliable profiles in all the studied samples (n = 460). The majority of results produced a single profile that matched the donor. Foreign DNA was detected in 15/46 victims, 61.5% positive detection in females vs 21.2% in males. A total of 38/390 DNA mixtures were detected in biological material under the fingernails being 19 from right hand (50%) and 19 from left hand (50%). The finger-like distribution was similar in right and left hands where the index, middle and ring fingers showed the 86.8% of the positive results; being these findings in line with the strength and shape of hand's fingers. The sample treatment optimization increased the possibilities of obtaining biological material from the perpetrator being a powerful tool in a murder investigation. Processing strategy including the penta-dactylar analysis of each hand for independent subungueal recovery of informative exogenous genetic material by fingernail clipping or swabbing and the use of optimized extraction and purification protocols might increase the rate of success in identifying criminal felons.
Fil: Caputo, Mariela. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Servicio de Huellas Digitales Genéticas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Canonaco, Enzo. Corte Suprema de Justicia de la Nación. Cuerpo Médico Forense; Argentina
Fil: Corach, Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Servicio de Huellas Digitales Genéticas; Argentina
Materia
Fingernail
Mixed Dna Profiles
Subungueal Genetic Material
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/38776
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Systematic analysis of decadactylar subungueal genetic traces in murder casesCaputo, MarielaCanonaco, EnzoCorach, DanielFingernailMixed Dna ProfilesSubungueal Genetic Materialhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Foreign biological material under the fingernails of a murder victim and/or suspect can be valuable evidence in a criminal investigation. The aim of this study was to evaluate the prevalence of foreign DNA under each fingernail of victims of violent deaths, including fatal struggle or rape cases. Fingernail swabs or, alternatively clipping nails, were taken from all fingers of both right and left hands from 46 victims (13 females and 33 males) and treated separately. Semi-automated DNA extraction was performed and 21 markers were genotyped. We detected reliable profiles in all the studied samples (n = 460). The majority of results produced a single profile that matched the donor. Foreign DNA was detected in 15/46 victims, 61.5% positive detection in females vs 21.2% in males. A total of 38/390 DNA mixtures were detected in biological material under the fingernails being 19 from right hand (50%) and 19 from left hand (50%). The finger-like distribution was similar in right and left hands where the index, middle and ring fingers showed the 86.8% of the positive results; being these findings in line with the strength and shape of hand's fingers. The sample treatment optimization increased the possibilities of obtaining biological material from the perpetrator being a powerful tool in a murder investigation. Processing strategy including the penta-dactylar analysis of each hand for independent subungueal recovery of informative exogenous genetic material by fingernail clipping or swabbing and the use of optimized extraction and purification protocols might increase the rate of success in identifying criminal felons.Fil: Caputo, Mariela. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Servicio de Huellas Digitales Genéticas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Canonaco, Enzo. Corte Suprema de Justicia de la Nación. Cuerpo Médico Forense; ArgentinaFil: Corach, Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Servicio de Huellas Digitales Genéticas; ArgentinaElsevier Ireland Ltd2015-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/38776Caputo, Mariela; Canonaco, Enzo; Corach, Daniel; Systematic analysis of decadactylar subungueal genetic traces in murder cases; Elsevier Ireland Ltd; Forensic Science International: Genetics Supplement Series; 5; 12-2015; e414-e4150379-07381875-175XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.fsigss.2015.09.164info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1875176815302158info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:46:34Zoai:ri.conicet.gov.ar:11336/38776instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:46:34.898CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Systematic analysis of decadactylar subungueal genetic traces in murder cases
title Systematic analysis of decadactylar subungueal genetic traces in murder cases
spellingShingle Systematic analysis of decadactylar subungueal genetic traces in murder cases
Caputo, Mariela
Fingernail
Mixed Dna Profiles
Subungueal Genetic Material
title_short Systematic analysis of decadactylar subungueal genetic traces in murder cases
title_full Systematic analysis of decadactylar subungueal genetic traces in murder cases
title_fullStr Systematic analysis of decadactylar subungueal genetic traces in murder cases
title_full_unstemmed Systematic analysis of decadactylar subungueal genetic traces in murder cases
title_sort Systematic analysis of decadactylar subungueal genetic traces in murder cases
dc.creator.none.fl_str_mv Caputo, Mariela
Canonaco, Enzo
Corach, Daniel
author Caputo, Mariela
author_facet Caputo, Mariela
Canonaco, Enzo
Corach, Daniel
author_role author
author2 Canonaco, Enzo
Corach, Daniel
author2_role author
author
dc.subject.none.fl_str_mv Fingernail
Mixed Dna Profiles
Subungueal Genetic Material
topic Fingernail
Mixed Dna Profiles
Subungueal Genetic Material
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Foreign biological material under the fingernails of a murder victim and/or suspect can be valuable evidence in a criminal investigation. The aim of this study was to evaluate the prevalence of foreign DNA under each fingernail of victims of violent deaths, including fatal struggle or rape cases. Fingernail swabs or, alternatively clipping nails, were taken from all fingers of both right and left hands from 46 victims (13 females and 33 males) and treated separately. Semi-automated DNA extraction was performed and 21 markers were genotyped. We detected reliable profiles in all the studied samples (n = 460). The majority of results produced a single profile that matched the donor. Foreign DNA was detected in 15/46 victims, 61.5% positive detection in females vs 21.2% in males. A total of 38/390 DNA mixtures were detected in biological material under the fingernails being 19 from right hand (50%) and 19 from left hand (50%). The finger-like distribution was similar in right and left hands where the index, middle and ring fingers showed the 86.8% of the positive results; being these findings in line with the strength and shape of hand's fingers. The sample treatment optimization increased the possibilities of obtaining biological material from the perpetrator being a powerful tool in a murder investigation. Processing strategy including the penta-dactylar analysis of each hand for independent subungueal recovery of informative exogenous genetic material by fingernail clipping or swabbing and the use of optimized extraction and purification protocols might increase the rate of success in identifying criminal felons.
Fil: Caputo, Mariela. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Servicio de Huellas Digitales Genéticas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Canonaco, Enzo. Corte Suprema de Justicia de la Nación. Cuerpo Médico Forense; Argentina
Fil: Corach, Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Servicio de Huellas Digitales Genéticas; Argentina
description Foreign biological material under the fingernails of a murder victim and/or suspect can be valuable evidence in a criminal investigation. The aim of this study was to evaluate the prevalence of foreign DNA under each fingernail of victims of violent deaths, including fatal struggle or rape cases. Fingernail swabs or, alternatively clipping nails, were taken from all fingers of both right and left hands from 46 victims (13 females and 33 males) and treated separately. Semi-automated DNA extraction was performed and 21 markers were genotyped. We detected reliable profiles in all the studied samples (n = 460). The majority of results produced a single profile that matched the donor. Foreign DNA was detected in 15/46 victims, 61.5% positive detection in females vs 21.2% in males. A total of 38/390 DNA mixtures were detected in biological material under the fingernails being 19 from right hand (50%) and 19 from left hand (50%). The finger-like distribution was similar in right and left hands where the index, middle and ring fingers showed the 86.8% of the positive results; being these findings in line with the strength and shape of hand's fingers. The sample treatment optimization increased the possibilities of obtaining biological material from the perpetrator being a powerful tool in a murder investigation. Processing strategy including the penta-dactylar analysis of each hand for independent subungueal recovery of informative exogenous genetic material by fingernail clipping or swabbing and the use of optimized extraction and purification protocols might increase the rate of success in identifying criminal felons.
publishDate 2015
dc.date.none.fl_str_mv 2015-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/38776
Caputo, Mariela; Canonaco, Enzo; Corach, Daniel; Systematic analysis of decadactylar subungueal genetic traces in murder cases; Elsevier Ireland Ltd; Forensic Science International: Genetics Supplement Series; 5; 12-2015; e414-e415
0379-0738
1875-175X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/38776
identifier_str_mv Caputo, Mariela; Canonaco, Enzo; Corach, Daniel; Systematic analysis of decadactylar subungueal genetic traces in murder cases; Elsevier Ireland Ltd; Forensic Science International: Genetics Supplement Series; 5; 12-2015; e414-e415
0379-0738
1875-175X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.fsigss.2015.09.164
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1875176815302158
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Ireland Ltd
publisher.none.fl_str_mv Elsevier Ireland Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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