A Transgenic Marker for Newly Born Granule Cells in Dentate Gyrus

Autores
Overstreet, Linda S.; Hentges, Shane T.; Bumaschny, Viviana Florencia; Silva Junqueira de Souza, Flavio; Smart, James L.; Santangelo, Andrea Mariana; Low, Malcolm J.; Westbrook, Gary L.; Rubinstein, Marcelo
Año de publicación
2004
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Neurogenesis in the dentate gyrus continues into adulthood, yet little is known about the function of newly born neurons or how they integrate into an existing network of mature neurons. We made transgenic mice that selectively and transiently express enhanced green fluorescent protein (EGFP) in newly born granule cells of the dentate gyrus under the transcriptional control of proopiomelanocortin (POMC) genomic sequences. Analysis of transgenic pedigrees with truncation or deletion mutations indicated that EGFP expression in the dentate gyrus required cryptic POMC promoter regions dispensable for arcuate hypothalamic or pituitary expression. Unlike arcuate neurons, dentate granule cells did not express the endogenous POMC gene. EGFP-positive neurons had immature properties, including short spineless dendrites and small action potentials. Colocalization with bromodeoxyuridine indicated that EGFP-labeled granule cells were ∼2 weeks postmitotic. EGFP-labeled cells expressed markers for immature granule cells but not the glial marker GFAP. The number of EGFP-labeled neurons declined with age and increased with exercise, paralleling neurogenesis. Our results indicate that POMC-EGFP marks immature granule cells and that adult-generated granule cells integrate quite slowly into the hippocampal circuitry.
Fil: Overstreet, Linda S.. Oregon Health and Science University; Estados Unidos
Fil: Hentges, Shane T.. Oregon Health and Science University; Estados Unidos
Fil: Bumaschny, Viviana Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular; Argentina
Fil: Silva Junqueira de Souza, Flavio. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular; Argentina
Fil: Smart, James L.. Oregon Health and Science University; Estados Unidos
Fil: Santangelo, Andrea Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular; Argentina
Fil: Low, Malcolm J.. Oregon Health and Science University; Estados Unidos
Fil: Westbrook, Gary L.. Oregon Health and Science University; Estados Unidos
Fil: Rubinstein, Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular; Argentina. Oregon Health and Science University; Estados Unidos. Centro de Estudios Cientificos; Chile
Materia
BRDU
DENTATE GRANULE CELL
GREEN FLUORESCENT PROTEIN
NEUROGENESIS
NEURONAL PROGENITOR
PROOPIOMELANOCORTIN
PSA-NCAM
TRANSGENIC MICE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/79742
Acceder
id CONICETDig_0fa0841886e3f05c06cff1ec37e3d372
oai_identifier_str oai:ri.conicet.gov.ar:11336/79742
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling A Transgenic Marker for Newly Born Granule Cells in Dentate GyrusOverstreet, Linda S.Hentges, Shane T.Bumaschny, Viviana FlorenciaSilva Junqueira de Souza, FlavioSmart, James L.Santangelo, Andrea MarianaLow, Malcolm J.Westbrook, Gary L.Rubinstein, MarceloBRDUDENTATE GRANULE CELLGREEN FLUORESCENT PROTEINNEUROGENESISNEURONAL PROGENITORPROOPIOMELANOCORTINPSA-NCAMTRANSGENIC MICEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Neurogenesis in the dentate gyrus continues into adulthood, yet little is known about the function of newly born neurons or how they integrate into an existing network of mature neurons. We made transgenic mice that selectively and transiently express enhanced green fluorescent protein (EGFP) in newly born granule cells of the dentate gyrus under the transcriptional control of proopiomelanocortin (POMC) genomic sequences. Analysis of transgenic pedigrees with truncation or deletion mutations indicated that EGFP expression in the dentate gyrus required cryptic POMC promoter regions dispensable for arcuate hypothalamic or pituitary expression. Unlike arcuate neurons, dentate granule cells did not express the endogenous POMC gene. EGFP-positive neurons had immature properties, including short spineless dendrites and small action potentials. Colocalization with bromodeoxyuridine indicated that EGFP-labeled granule cells were ∼2 weeks postmitotic. EGFP-labeled cells expressed markers for immature granule cells but not the glial marker GFAP. The number of EGFP-labeled neurons declined with age and increased with exercise, paralleling neurogenesis. Our results indicate that POMC-EGFP marks immature granule cells and that adult-generated granule cells integrate quite slowly into the hippocampal circuitry.Fil: Overstreet, Linda S.. Oregon Health and Science University; Estados UnidosFil: Hentges, Shane T.. Oregon Health and Science University; Estados UnidosFil: Bumaschny, Viviana Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular; ArgentinaFil: Silva Junqueira de Souza, Flavio. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular; ArgentinaFil: Smart, James L.. Oregon Health and Science University; Estados UnidosFil: Santangelo, Andrea Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular; ArgentinaFil: Low, Malcolm J.. Oregon Health and Science University; Estados UnidosFil: Westbrook, Gary L.. Oregon Health and Science University; Estados UnidosFil: Rubinstein, Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular; Argentina. Oregon Health and Science University; Estados Unidos. Centro de Estudios Cientificos; ChileSociety for Neuroscience2004-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/79742Overstreet, Linda S.; Hentges, Shane T.; Bumaschny, Viviana Florencia; Silva Junqueira de Souza, Flavio; Smart, James L.; et al.; A Transgenic Marker for Newly Born Granule Cells in Dentate Gyrus; Society for Neuroscience; Journal of Neuroscience; 24; 13; 3-2004; 3251-32590270-6474CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pubmed/15056704info:eu-repo/semantics/altIdentifier/doi/10.1523/JNEUROSCI.5173-03.2004info:eu-repo/semantics/altIdentifier/url/https://www.jneurosci.org/content/24/13/3251info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:37:13Zoai:ri.conicet.gov.ar:11336/79742instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:37:13.983CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv A Transgenic Marker for Newly Born Granule Cells in Dentate Gyrus
title A Transgenic Marker for Newly Born Granule Cells in Dentate Gyrus
spellingShingle A Transgenic Marker for Newly Born Granule Cells in Dentate Gyrus
Overstreet, Linda S.
BRDU
DENTATE GRANULE CELL
GREEN FLUORESCENT PROTEIN
NEUROGENESIS
NEURONAL PROGENITOR
PROOPIOMELANOCORTIN
PSA-NCAM
TRANSGENIC MICE
title_short A Transgenic Marker for Newly Born Granule Cells in Dentate Gyrus
title_full A Transgenic Marker for Newly Born Granule Cells in Dentate Gyrus
title_fullStr A Transgenic Marker for Newly Born Granule Cells in Dentate Gyrus
title_full_unstemmed A Transgenic Marker for Newly Born Granule Cells in Dentate Gyrus
title_sort A Transgenic Marker for Newly Born Granule Cells in Dentate Gyrus
dc.creator.none.fl_str_mv Overstreet, Linda S.
Hentges, Shane T.
Bumaschny, Viviana Florencia
Silva Junqueira de Souza, Flavio
Smart, James L.
Santangelo, Andrea Mariana
Low, Malcolm J.
Westbrook, Gary L.
Rubinstein, Marcelo
author Overstreet, Linda S.
author_facet Overstreet, Linda S.
Hentges, Shane T.
Bumaschny, Viviana Florencia
Silva Junqueira de Souza, Flavio
Smart, James L.
Santangelo, Andrea Mariana
Low, Malcolm J.
Westbrook, Gary L.
Rubinstein, Marcelo
author_role author
author2 Hentges, Shane T.
Bumaschny, Viviana Florencia
Silva Junqueira de Souza, Flavio
Smart, James L.
Santangelo, Andrea Mariana
Low, Malcolm J.
Westbrook, Gary L.
Rubinstein, Marcelo
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv BRDU
DENTATE GRANULE CELL
GREEN FLUORESCENT PROTEIN
NEUROGENESIS
NEURONAL PROGENITOR
PROOPIOMELANOCORTIN
PSA-NCAM
TRANSGENIC MICE
topic BRDU
DENTATE GRANULE CELL
GREEN FLUORESCENT PROTEIN
NEUROGENESIS
NEURONAL PROGENITOR
PROOPIOMELANOCORTIN
PSA-NCAM
TRANSGENIC MICE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Neurogenesis in the dentate gyrus continues into adulthood, yet little is known about the function of newly born neurons or how they integrate into an existing network of mature neurons. We made transgenic mice that selectively and transiently express enhanced green fluorescent protein (EGFP) in newly born granule cells of the dentate gyrus under the transcriptional control of proopiomelanocortin (POMC) genomic sequences. Analysis of transgenic pedigrees with truncation or deletion mutations indicated that EGFP expression in the dentate gyrus required cryptic POMC promoter regions dispensable for arcuate hypothalamic or pituitary expression. Unlike arcuate neurons, dentate granule cells did not express the endogenous POMC gene. EGFP-positive neurons had immature properties, including short spineless dendrites and small action potentials. Colocalization with bromodeoxyuridine indicated that EGFP-labeled granule cells were ∼2 weeks postmitotic. EGFP-labeled cells expressed markers for immature granule cells but not the glial marker GFAP. The number of EGFP-labeled neurons declined with age and increased with exercise, paralleling neurogenesis. Our results indicate that POMC-EGFP marks immature granule cells and that adult-generated granule cells integrate quite slowly into the hippocampal circuitry.
Fil: Overstreet, Linda S.. Oregon Health and Science University; Estados Unidos
Fil: Hentges, Shane T.. Oregon Health and Science University; Estados Unidos
Fil: Bumaschny, Viviana Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular; Argentina
Fil: Silva Junqueira de Souza, Flavio. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular; Argentina
Fil: Smart, James L.. Oregon Health and Science University; Estados Unidos
Fil: Santangelo, Andrea Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular; Argentina
Fil: Low, Malcolm J.. Oregon Health and Science University; Estados Unidos
Fil: Westbrook, Gary L.. Oregon Health and Science University; Estados Unidos
Fil: Rubinstein, Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular; Argentina. Oregon Health and Science University; Estados Unidos. Centro de Estudios Cientificos; Chile
description Neurogenesis in the dentate gyrus continues into adulthood, yet little is known about the function of newly born neurons or how they integrate into an existing network of mature neurons. We made transgenic mice that selectively and transiently express enhanced green fluorescent protein (EGFP) in newly born granule cells of the dentate gyrus under the transcriptional control of proopiomelanocortin (POMC) genomic sequences. Analysis of transgenic pedigrees with truncation or deletion mutations indicated that EGFP expression in the dentate gyrus required cryptic POMC promoter regions dispensable for arcuate hypothalamic or pituitary expression. Unlike arcuate neurons, dentate granule cells did not express the endogenous POMC gene. EGFP-positive neurons had immature properties, including short spineless dendrites and small action potentials. Colocalization with bromodeoxyuridine indicated that EGFP-labeled granule cells were ∼2 weeks postmitotic. EGFP-labeled cells expressed markers for immature granule cells but not the glial marker GFAP. The number of EGFP-labeled neurons declined with age and increased with exercise, paralleling neurogenesis. Our results indicate that POMC-EGFP marks immature granule cells and that adult-generated granule cells integrate quite slowly into the hippocampal circuitry.
publishDate 2004
dc.date.none.fl_str_mv 2004-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/79742
Overstreet, Linda S.; Hentges, Shane T.; Bumaschny, Viviana Florencia; Silva Junqueira de Souza, Flavio; Smart, James L.; et al.; A Transgenic Marker for Newly Born Granule Cells in Dentate Gyrus; Society for Neuroscience; Journal of Neuroscience; 24; 13; 3-2004; 3251-3259
0270-6474
CONICET Digital
CONICET
url http://hdl.handle.net/11336/79742
identifier_str_mv Overstreet, Linda S.; Hentges, Shane T.; Bumaschny, Viviana Florencia; Silva Junqueira de Souza, Flavio; Smart, James L.; et al.; A Transgenic Marker for Newly Born Granule Cells in Dentate Gyrus; Society for Neuroscience; Journal of Neuroscience; 24; 13; 3-2004; 3251-3259
0270-6474
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pubmed/15056704
info:eu-repo/semantics/altIdentifier/doi/10.1523/JNEUROSCI.5173-03.2004
info:eu-repo/semantics/altIdentifier/url/https://www.jneurosci.org/content/24/13/3251
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Society for Neuroscience
publisher.none.fl_str_mv Society for Neuroscience
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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