A Transgenic Marker for Newly Born Granule Cells in Dentate Gyrus
- Autores
- Overstreet, Linda S.; Hentges, Shane T.; Bumaschny, Viviana Florencia; Silva Junqueira de Souza, Flavio; Smart, James L.; Santangelo, Andrea Mariana; Low, Malcolm J.; Westbrook, Gary L.; Rubinstein, Marcelo
- Año de publicación
- 2004
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Neurogenesis in the dentate gyrus continues into adulthood, yet little is known about the function of newly born neurons or how they integrate into an existing network of mature neurons. We made transgenic mice that selectively and transiently express enhanced green fluorescent protein (EGFP) in newly born granule cells of the dentate gyrus under the transcriptional control of proopiomelanocortin (POMC) genomic sequences. Analysis of transgenic pedigrees with truncation or deletion mutations indicated that EGFP expression in the dentate gyrus required cryptic POMC promoter regions dispensable for arcuate hypothalamic or pituitary expression. Unlike arcuate neurons, dentate granule cells did not express the endogenous POMC gene. EGFP-positive neurons had immature properties, including short spineless dendrites and small action potentials. Colocalization with bromodeoxyuridine indicated that EGFP-labeled granule cells were ∼2 weeks postmitotic. EGFP-labeled cells expressed markers for immature granule cells but not the glial marker GFAP. The number of EGFP-labeled neurons declined with age and increased with exercise, paralleling neurogenesis. Our results indicate that POMC-EGFP marks immature granule cells and that adult-generated granule cells integrate quite slowly into the hippocampal circuitry.
Fil: Overstreet, Linda S.. Oregon Health and Science University; Estados Unidos
Fil: Hentges, Shane T.. Oregon Health and Science University; Estados Unidos
Fil: Bumaschny, Viviana Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular; Argentina
Fil: Silva Junqueira de Souza, Flavio. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular; Argentina
Fil: Smart, James L.. Oregon Health and Science University; Estados Unidos
Fil: Santangelo, Andrea Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular; Argentina
Fil: Low, Malcolm J.. Oregon Health and Science University; Estados Unidos
Fil: Westbrook, Gary L.. Oregon Health and Science University; Estados Unidos
Fil: Rubinstein, Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular; Argentina. Oregon Health and Science University; Estados Unidos. Centro de Estudios Cientificos; Chile - Materia
-
BRDU
DENTATE GRANULE CELL
GREEN FLUORESCENT PROTEIN
NEUROGENESIS
NEURONAL PROGENITOR
PROOPIOMELANOCORTIN
PSA-NCAM
TRANSGENIC MICE - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/79742
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oai:ri.conicet.gov.ar:11336/79742 |
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A Transgenic Marker for Newly Born Granule Cells in Dentate GyrusOverstreet, Linda S.Hentges, Shane T.Bumaschny, Viviana FlorenciaSilva Junqueira de Souza, FlavioSmart, James L.Santangelo, Andrea MarianaLow, Malcolm J.Westbrook, Gary L.Rubinstein, MarceloBRDUDENTATE GRANULE CELLGREEN FLUORESCENT PROTEINNEUROGENESISNEURONAL PROGENITORPROOPIOMELANOCORTINPSA-NCAMTRANSGENIC MICEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Neurogenesis in the dentate gyrus continues into adulthood, yet little is known about the function of newly born neurons or how they integrate into an existing network of mature neurons. We made transgenic mice that selectively and transiently express enhanced green fluorescent protein (EGFP) in newly born granule cells of the dentate gyrus under the transcriptional control of proopiomelanocortin (POMC) genomic sequences. Analysis of transgenic pedigrees with truncation or deletion mutations indicated that EGFP expression in the dentate gyrus required cryptic POMC promoter regions dispensable for arcuate hypothalamic or pituitary expression. Unlike arcuate neurons, dentate granule cells did not express the endogenous POMC gene. EGFP-positive neurons had immature properties, including short spineless dendrites and small action potentials. Colocalization with bromodeoxyuridine indicated that EGFP-labeled granule cells were ∼2 weeks postmitotic. EGFP-labeled cells expressed markers for immature granule cells but not the glial marker GFAP. The number of EGFP-labeled neurons declined with age and increased with exercise, paralleling neurogenesis. Our results indicate that POMC-EGFP marks immature granule cells and that adult-generated granule cells integrate quite slowly into the hippocampal circuitry.Fil: Overstreet, Linda S.. Oregon Health and Science University; Estados UnidosFil: Hentges, Shane T.. Oregon Health and Science University; Estados UnidosFil: Bumaschny, Viviana Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular; ArgentinaFil: Silva Junqueira de Souza, Flavio. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular; ArgentinaFil: Smart, James L.. Oregon Health and Science University; Estados UnidosFil: Santangelo, Andrea Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular; ArgentinaFil: Low, Malcolm J.. Oregon Health and Science University; Estados UnidosFil: Westbrook, Gary L.. Oregon Health and Science University; Estados UnidosFil: Rubinstein, Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular; Argentina. Oregon Health and Science University; Estados Unidos. Centro de Estudios Cientificos; ChileSociety for Neuroscience2004-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/79742Overstreet, Linda S.; Hentges, Shane T.; Bumaschny, Viviana Florencia; Silva Junqueira de Souza, Flavio; Smart, James L.; et al.; A Transgenic Marker for Newly Born Granule Cells in Dentate Gyrus; Society for Neuroscience; Journal of Neuroscience; 24; 13; 3-2004; 3251-32590270-6474CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pubmed/15056704info:eu-repo/semantics/altIdentifier/doi/10.1523/JNEUROSCI.5173-03.2004info:eu-repo/semantics/altIdentifier/url/https://www.jneurosci.org/content/24/13/3251info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:08:29Zoai:ri.conicet.gov.ar:11336/79742instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:08:29.735CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
A Transgenic Marker for Newly Born Granule Cells in Dentate Gyrus |
title |
A Transgenic Marker for Newly Born Granule Cells in Dentate Gyrus |
spellingShingle |
A Transgenic Marker for Newly Born Granule Cells in Dentate Gyrus Overstreet, Linda S. BRDU DENTATE GRANULE CELL GREEN FLUORESCENT PROTEIN NEUROGENESIS NEURONAL PROGENITOR PROOPIOMELANOCORTIN PSA-NCAM TRANSGENIC MICE |
title_short |
A Transgenic Marker for Newly Born Granule Cells in Dentate Gyrus |
title_full |
A Transgenic Marker for Newly Born Granule Cells in Dentate Gyrus |
title_fullStr |
A Transgenic Marker for Newly Born Granule Cells in Dentate Gyrus |
title_full_unstemmed |
A Transgenic Marker for Newly Born Granule Cells in Dentate Gyrus |
title_sort |
A Transgenic Marker for Newly Born Granule Cells in Dentate Gyrus |
dc.creator.none.fl_str_mv |
Overstreet, Linda S. Hentges, Shane T. Bumaschny, Viviana Florencia Silva Junqueira de Souza, Flavio Smart, James L. Santangelo, Andrea Mariana Low, Malcolm J. Westbrook, Gary L. Rubinstein, Marcelo |
author |
Overstreet, Linda S. |
author_facet |
Overstreet, Linda S. Hentges, Shane T. Bumaschny, Viviana Florencia Silva Junqueira de Souza, Flavio Smart, James L. Santangelo, Andrea Mariana Low, Malcolm J. Westbrook, Gary L. Rubinstein, Marcelo |
author_role |
author |
author2 |
Hentges, Shane T. Bumaschny, Viviana Florencia Silva Junqueira de Souza, Flavio Smart, James L. Santangelo, Andrea Mariana Low, Malcolm J. Westbrook, Gary L. Rubinstein, Marcelo |
author2_role |
author author author author author author author author |
dc.subject.none.fl_str_mv |
BRDU DENTATE GRANULE CELL GREEN FLUORESCENT PROTEIN NEUROGENESIS NEURONAL PROGENITOR PROOPIOMELANOCORTIN PSA-NCAM TRANSGENIC MICE |
topic |
BRDU DENTATE GRANULE CELL GREEN FLUORESCENT PROTEIN NEUROGENESIS NEURONAL PROGENITOR PROOPIOMELANOCORTIN PSA-NCAM TRANSGENIC MICE |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Neurogenesis in the dentate gyrus continues into adulthood, yet little is known about the function of newly born neurons or how they integrate into an existing network of mature neurons. We made transgenic mice that selectively and transiently express enhanced green fluorescent protein (EGFP) in newly born granule cells of the dentate gyrus under the transcriptional control of proopiomelanocortin (POMC) genomic sequences. Analysis of transgenic pedigrees with truncation or deletion mutations indicated that EGFP expression in the dentate gyrus required cryptic POMC promoter regions dispensable for arcuate hypothalamic or pituitary expression. Unlike arcuate neurons, dentate granule cells did not express the endogenous POMC gene. EGFP-positive neurons had immature properties, including short spineless dendrites and small action potentials. Colocalization with bromodeoxyuridine indicated that EGFP-labeled granule cells were ∼2 weeks postmitotic. EGFP-labeled cells expressed markers for immature granule cells but not the glial marker GFAP. The number of EGFP-labeled neurons declined with age and increased with exercise, paralleling neurogenesis. Our results indicate that POMC-EGFP marks immature granule cells and that adult-generated granule cells integrate quite slowly into the hippocampal circuitry. Fil: Overstreet, Linda S.. Oregon Health and Science University; Estados Unidos Fil: Hentges, Shane T.. Oregon Health and Science University; Estados Unidos Fil: Bumaschny, Viviana Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular; Argentina Fil: Silva Junqueira de Souza, Flavio. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular; Argentina Fil: Smart, James L.. Oregon Health and Science University; Estados Unidos Fil: Santangelo, Andrea Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular; Argentina Fil: Low, Malcolm J.. Oregon Health and Science University; Estados Unidos Fil: Westbrook, Gary L.. Oregon Health and Science University; Estados Unidos Fil: Rubinstein, Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular; Argentina. Oregon Health and Science University; Estados Unidos. Centro de Estudios Cientificos; Chile |
description |
Neurogenesis in the dentate gyrus continues into adulthood, yet little is known about the function of newly born neurons or how they integrate into an existing network of mature neurons. We made transgenic mice that selectively and transiently express enhanced green fluorescent protein (EGFP) in newly born granule cells of the dentate gyrus under the transcriptional control of proopiomelanocortin (POMC) genomic sequences. Analysis of transgenic pedigrees with truncation or deletion mutations indicated that EGFP expression in the dentate gyrus required cryptic POMC promoter regions dispensable for arcuate hypothalamic or pituitary expression. Unlike arcuate neurons, dentate granule cells did not express the endogenous POMC gene. EGFP-positive neurons had immature properties, including short spineless dendrites and small action potentials. Colocalization with bromodeoxyuridine indicated that EGFP-labeled granule cells were ∼2 weeks postmitotic. EGFP-labeled cells expressed markers for immature granule cells but not the glial marker GFAP. The number of EGFP-labeled neurons declined with age and increased with exercise, paralleling neurogenesis. Our results indicate that POMC-EGFP marks immature granule cells and that adult-generated granule cells integrate quite slowly into the hippocampal circuitry. |
publishDate |
2004 |
dc.date.none.fl_str_mv |
2004-03 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/79742 Overstreet, Linda S.; Hentges, Shane T.; Bumaschny, Viviana Florencia; Silva Junqueira de Souza, Flavio; Smart, James L.; et al.; A Transgenic Marker for Newly Born Granule Cells in Dentate Gyrus; Society for Neuroscience; Journal of Neuroscience; 24; 13; 3-2004; 3251-3259 0270-6474 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/79742 |
identifier_str_mv |
Overstreet, Linda S.; Hentges, Shane T.; Bumaschny, Viviana Florencia; Silva Junqueira de Souza, Flavio; Smart, James L.; et al.; A Transgenic Marker for Newly Born Granule Cells in Dentate Gyrus; Society for Neuroscience; Journal of Neuroscience; 24; 13; 3-2004; 3251-3259 0270-6474 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pubmed/15056704 info:eu-repo/semantics/altIdentifier/doi/10.1523/JNEUROSCI.5173-03.2004 info:eu-repo/semantics/altIdentifier/url/https://www.jneurosci.org/content/24/13/3251 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Society for Neuroscience |
publisher.none.fl_str_mv |
Society for Neuroscience |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1842270047150538752 |
score |
13.13397 |