Arabidopsis expressing a cyanobacterial nitric oxide synthase increased yield even under uv-b exposure

Autores
del Castello, Fiorella Paola; Latorre, Lucas Leonel; Nejamkin, Andres; Foresi, Noelia Pamela; Correa Aragunde, Maria Natalia
Año de publicación
2021
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
The enzyme nitric oxide synthase (NOS) catalyzes the biosynthesis of nitric oxide (NO) and citrulline from the substrate arginine. The NOS from the cyanobacteria Synechococcuss PCC 7335 (SyNOS) possesses the oxygenase and reductase domains like animal NOS, but it has an extra domain encoding a globin at the N terminus. In vitro assays demonstrated that the globin domain of SyNOS acts as a NO dioxygenase, oxidizing the NO to nitrate. As a result, SyNOS is able to produce NO from arginine and also to oxidize it to nitrate with a release rate 75:25 (nitrate:NO). Thus, our hypothesis is that SyNOS expression in plants may allow a greater remobilization of internal nitrogen (N), improving growth and yield. Furthermore, both nitrate and NO produced by SyNOS may positively affect the signaling of various growth processes and/or responses to stresses. Recently, we showed that heterologous expression of SyNOS in Arabidopsis improves N use efficiency, N-deficiency tolerance and yield. In this work, we evaluate the response of SyNOS-expressing Arabidopsis plants to UV-B exposure. Our results show that UV induces morphological changes in UV acclimated plants (exposed to UV-B 1.1 W.m-2, 2 h per day during 14 days) which include decreased rosette diameter, decreased inflorescence height, increased numbers of flowering stems and decreased numbers of secondary branches. These UV-induced morphological changes were observed in both transgenic SyNOS and Rdr-6 control plants. However, the transgenic lines presented increased shoot branching and seed production compared to Rdr-6 plants in both conditions (with and without UV-B exposure). Chronic UV-B radiation did not affect flavonoid pigment levels, cell damage or ROS production, indicating that UV-acclimated plants were not stressed. Additionally, the effect of prolonged UV-B exposure (irradiated with UV-B 0.5 W.m-2 during 6 days) was analyzed in seedlings grown in nutrient agar medium with high (+N, 9 mM NO3-) and low N (-N, 0.5 mM NO3-) conditions. UV-B irradiation as well as -N condition inhibited hypocotyl elongation in all lines. In -N condition without UV-B SyNOS lines elongated more the hypocotyl compared to control plants. Under UV-B exposure, increased hypocotyl elongation of transgenic lines was observed only in +N condition. Further investigation is necessary to understand the UV-B acclimation response of transgenic SyNOS lines. Acclimation to a state of stress combination has been shown to involve integrating responses to each of the individual stresses that simultaneously impact the plant (e.g., low N or UV-B stress), as well as the induction of a new type of response, sometimes involving thousands of transcripts, that is unique to the state of stress combination. In summary our results are encouraging towards obtaining crops with better yield under combined stressful conditions.
Fil: del Castello, Fiorella Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Latorre, Lucas Leonel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Nejamkin, Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Foresi, Noelia Pamela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Correa Aragunde, Maria Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Congreso Conjunto SAIB-SAIGE 2021: LVII Reunión Anual de la Sociedad Argentina de Investigaciones en Bioquímica y Biología Molecular (SAIB). XVI Congreso Anual de la Asociación Civil de Microbiología General (SAMIGE)
Argentina
Sociedad Argentina de Investigaciones en Bioquímica y Biología Molecular
Asociación Civil de Microbiología General
Materia
NITROC OXIDE SYNTHASE
ARABIDOPSIS
CYANOBACTERIA
UV-B STRESS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/217106

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network_name_str CONICET Digital (CONICET)
spelling Arabidopsis expressing a cyanobacterial nitric oxide synthase increased yield even under uv-b exposuredel Castello, Fiorella PaolaLatorre, Lucas LeonelNejamkin, AndresForesi, Noelia PamelaCorrea Aragunde, Maria NataliaNITROC OXIDE SYNTHASEARABIDOPSISCYANOBACTERIAUV-B STRESShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The enzyme nitric oxide synthase (NOS) catalyzes the biosynthesis of nitric oxide (NO) and citrulline from the substrate arginine. The NOS from the cyanobacteria Synechococcuss PCC 7335 (SyNOS) possesses the oxygenase and reductase domains like animal NOS, but it has an extra domain encoding a globin at the N terminus. In vitro assays demonstrated that the globin domain of SyNOS acts as a NO dioxygenase, oxidizing the NO to nitrate. As a result, SyNOS is able to produce NO from arginine and also to oxidize it to nitrate with a release rate 75:25 (nitrate:NO). Thus, our hypothesis is that SyNOS expression in plants may allow a greater remobilization of internal nitrogen (N), improving growth and yield. Furthermore, both nitrate and NO produced by SyNOS may positively affect the signaling of various growth processes and/or responses to stresses. Recently, we showed that heterologous expression of SyNOS in Arabidopsis improves N use efficiency, N-deficiency tolerance and yield. In this work, we evaluate the response of SyNOS-expressing Arabidopsis plants to UV-B exposure. Our results show that UV induces morphological changes in UV acclimated plants (exposed to UV-B 1.1 W.m-2, 2 h per day during 14 days) which include decreased rosette diameter, decreased inflorescence height, increased numbers of flowering stems and decreased numbers of secondary branches. These UV-induced morphological changes were observed in both transgenic SyNOS and Rdr-6 control plants. However, the transgenic lines presented increased shoot branching and seed production compared to Rdr-6 plants in both conditions (with and without UV-B exposure). Chronic UV-B radiation did not affect flavonoid pigment levels, cell damage or ROS production, indicating that UV-acclimated plants were not stressed. Additionally, the effect of prolonged UV-B exposure (irradiated with UV-B 0.5 W.m-2 during 6 days) was analyzed in seedlings grown in nutrient agar medium with high (+N, 9 mM NO3-) and low N (-N, 0.5 mM NO3-) conditions. UV-B irradiation as well as -N condition inhibited hypocotyl elongation in all lines. In -N condition without UV-B SyNOS lines elongated more the hypocotyl compared to control plants. Under UV-B exposure, increased hypocotyl elongation of transgenic lines was observed only in +N condition. Further investigation is necessary to understand the UV-B acclimation response of transgenic SyNOS lines. Acclimation to a state of stress combination has been shown to involve integrating responses to each of the individual stresses that simultaneously impact the plant (e.g., low N or UV-B stress), as well as the induction of a new type of response, sometimes involving thousands of transcripts, that is unique to the state of stress combination. In summary our results are encouraging towards obtaining crops with better yield under combined stressful conditions.Fil: del Castello, Fiorella Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; ArgentinaFil: Latorre, Lucas Leonel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; ArgentinaFil: Nejamkin, Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; ArgentinaFil: Foresi, Noelia Pamela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; ArgentinaFil: Correa Aragunde, Maria Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; ArgentinaCongreso Conjunto SAIB-SAIGE 2021: LVII Reunión Anual de la Sociedad Argentina de Investigaciones en Bioquímica y Biología Molecular (SAIB). XVI Congreso Anual de la Asociación Civil de Microbiología General (SAMIGE)ArgentinaSociedad Argentina de Investigaciones en Bioquímica y Biología MolecularAsociación Civil de Microbiología GeneralTech Science Press2021info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/217106Arabidopsis expressing a cyanobacterial nitric oxide synthase increased yield even under uv-b exposure; Congreso Conjunto SAIB-SAIGE 2021: LVII Reunión Anual de la Sociedad Argentina de Investigaciones en Bioquímica y Biología Molecular (SAIB). XVI Congreso Anual de la Asociación Civil de Microbiología General (SAMIGE); Argentina; 2021; 177-1780327-95451667-5746CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.saib.org.ar/sites/default/files/TSP_BIOCELL_46213-SAIB-SAMIGE%202021.pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:31:18Zoai:ri.conicet.gov.ar:11336/217106instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:31:19.212CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Arabidopsis expressing a cyanobacterial nitric oxide synthase increased yield even under uv-b exposure
title Arabidopsis expressing a cyanobacterial nitric oxide synthase increased yield even under uv-b exposure
spellingShingle Arabidopsis expressing a cyanobacterial nitric oxide synthase increased yield even under uv-b exposure
del Castello, Fiorella Paola
NITROC OXIDE SYNTHASE
ARABIDOPSIS
CYANOBACTERIA
UV-B STRESS
title_short Arabidopsis expressing a cyanobacterial nitric oxide synthase increased yield even under uv-b exposure
title_full Arabidopsis expressing a cyanobacterial nitric oxide synthase increased yield even under uv-b exposure
title_fullStr Arabidopsis expressing a cyanobacterial nitric oxide synthase increased yield even under uv-b exposure
title_full_unstemmed Arabidopsis expressing a cyanobacterial nitric oxide synthase increased yield even under uv-b exposure
title_sort Arabidopsis expressing a cyanobacterial nitric oxide synthase increased yield even under uv-b exposure
dc.creator.none.fl_str_mv del Castello, Fiorella Paola
Latorre, Lucas Leonel
Nejamkin, Andres
Foresi, Noelia Pamela
Correa Aragunde, Maria Natalia
author del Castello, Fiorella Paola
author_facet del Castello, Fiorella Paola
Latorre, Lucas Leonel
Nejamkin, Andres
Foresi, Noelia Pamela
Correa Aragunde, Maria Natalia
author_role author
author2 Latorre, Lucas Leonel
Nejamkin, Andres
Foresi, Noelia Pamela
Correa Aragunde, Maria Natalia
author2_role author
author
author
author
dc.subject.none.fl_str_mv NITROC OXIDE SYNTHASE
ARABIDOPSIS
CYANOBACTERIA
UV-B STRESS
topic NITROC OXIDE SYNTHASE
ARABIDOPSIS
CYANOBACTERIA
UV-B STRESS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The enzyme nitric oxide synthase (NOS) catalyzes the biosynthesis of nitric oxide (NO) and citrulline from the substrate arginine. The NOS from the cyanobacteria Synechococcuss PCC 7335 (SyNOS) possesses the oxygenase and reductase domains like animal NOS, but it has an extra domain encoding a globin at the N terminus. In vitro assays demonstrated that the globin domain of SyNOS acts as a NO dioxygenase, oxidizing the NO to nitrate. As a result, SyNOS is able to produce NO from arginine and also to oxidize it to nitrate with a release rate 75:25 (nitrate:NO). Thus, our hypothesis is that SyNOS expression in plants may allow a greater remobilization of internal nitrogen (N), improving growth and yield. Furthermore, both nitrate and NO produced by SyNOS may positively affect the signaling of various growth processes and/or responses to stresses. Recently, we showed that heterologous expression of SyNOS in Arabidopsis improves N use efficiency, N-deficiency tolerance and yield. In this work, we evaluate the response of SyNOS-expressing Arabidopsis plants to UV-B exposure. Our results show that UV induces morphological changes in UV acclimated plants (exposed to UV-B 1.1 W.m-2, 2 h per day during 14 days) which include decreased rosette diameter, decreased inflorescence height, increased numbers of flowering stems and decreased numbers of secondary branches. These UV-induced morphological changes were observed in both transgenic SyNOS and Rdr-6 control plants. However, the transgenic lines presented increased shoot branching and seed production compared to Rdr-6 plants in both conditions (with and without UV-B exposure). Chronic UV-B radiation did not affect flavonoid pigment levels, cell damage or ROS production, indicating that UV-acclimated plants were not stressed. Additionally, the effect of prolonged UV-B exposure (irradiated with UV-B 0.5 W.m-2 during 6 days) was analyzed in seedlings grown in nutrient agar medium with high (+N, 9 mM NO3-) and low N (-N, 0.5 mM NO3-) conditions. UV-B irradiation as well as -N condition inhibited hypocotyl elongation in all lines. In -N condition without UV-B SyNOS lines elongated more the hypocotyl compared to control plants. Under UV-B exposure, increased hypocotyl elongation of transgenic lines was observed only in +N condition. Further investigation is necessary to understand the UV-B acclimation response of transgenic SyNOS lines. Acclimation to a state of stress combination has been shown to involve integrating responses to each of the individual stresses that simultaneously impact the plant (e.g., low N or UV-B stress), as well as the induction of a new type of response, sometimes involving thousands of transcripts, that is unique to the state of stress combination. In summary our results are encouraging towards obtaining crops with better yield under combined stressful conditions.
Fil: del Castello, Fiorella Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Latorre, Lucas Leonel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Nejamkin, Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Foresi, Noelia Pamela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Correa Aragunde, Maria Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Congreso Conjunto SAIB-SAIGE 2021: LVII Reunión Anual de la Sociedad Argentina de Investigaciones en Bioquímica y Biología Molecular (SAIB). XVI Congreso Anual de la Asociación Civil de Microbiología General (SAMIGE)
Argentina
Sociedad Argentina de Investigaciones en Bioquímica y Biología Molecular
Asociación Civil de Microbiología General
description The enzyme nitric oxide synthase (NOS) catalyzes the biosynthesis of nitric oxide (NO) and citrulline from the substrate arginine. The NOS from the cyanobacteria Synechococcuss PCC 7335 (SyNOS) possesses the oxygenase and reductase domains like animal NOS, but it has an extra domain encoding a globin at the N terminus. In vitro assays demonstrated that the globin domain of SyNOS acts as a NO dioxygenase, oxidizing the NO to nitrate. As a result, SyNOS is able to produce NO from arginine and also to oxidize it to nitrate with a release rate 75:25 (nitrate:NO). Thus, our hypothesis is that SyNOS expression in plants may allow a greater remobilization of internal nitrogen (N), improving growth and yield. Furthermore, both nitrate and NO produced by SyNOS may positively affect the signaling of various growth processes and/or responses to stresses. Recently, we showed that heterologous expression of SyNOS in Arabidopsis improves N use efficiency, N-deficiency tolerance and yield. In this work, we evaluate the response of SyNOS-expressing Arabidopsis plants to UV-B exposure. Our results show that UV induces morphological changes in UV acclimated plants (exposed to UV-B 1.1 W.m-2, 2 h per day during 14 days) which include decreased rosette diameter, decreased inflorescence height, increased numbers of flowering stems and decreased numbers of secondary branches. These UV-induced morphological changes were observed in both transgenic SyNOS and Rdr-6 control plants. However, the transgenic lines presented increased shoot branching and seed production compared to Rdr-6 plants in both conditions (with and without UV-B exposure). Chronic UV-B radiation did not affect flavonoid pigment levels, cell damage or ROS production, indicating that UV-acclimated plants were not stressed. Additionally, the effect of prolonged UV-B exposure (irradiated with UV-B 0.5 W.m-2 during 6 days) was analyzed in seedlings grown in nutrient agar medium with high (+N, 9 mM NO3-) and low N (-N, 0.5 mM NO3-) conditions. UV-B irradiation as well as -N condition inhibited hypocotyl elongation in all lines. In -N condition without UV-B SyNOS lines elongated more the hypocotyl compared to control plants. Under UV-B exposure, increased hypocotyl elongation of transgenic lines was observed only in +N condition. Further investigation is necessary to understand the UV-B acclimation response of transgenic SyNOS lines. Acclimation to a state of stress combination has been shown to involve integrating responses to each of the individual stresses that simultaneously impact the plant (e.g., low N or UV-B stress), as well as the induction of a new type of response, sometimes involving thousands of transcripts, that is unique to the state of stress combination. In summary our results are encouraging towards obtaining crops with better yield under combined stressful conditions.
publishDate 2021
dc.date.none.fl_str_mv 2021
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dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/217106
Arabidopsis expressing a cyanobacterial nitric oxide synthase increased yield even under uv-b exposure; Congreso Conjunto SAIB-SAIGE 2021: LVII Reunión Anual de la Sociedad Argentina de Investigaciones en Bioquímica y Biología Molecular (SAIB). XVI Congreso Anual de la Asociación Civil de Microbiología General (SAMIGE); Argentina; 2021; 177-178
0327-9545
1667-5746
CONICET Digital
CONICET
url http://hdl.handle.net/11336/217106
identifier_str_mv Arabidopsis expressing a cyanobacterial nitric oxide synthase increased yield even under uv-b exposure; Congreso Conjunto SAIB-SAIGE 2021: LVII Reunión Anual de la Sociedad Argentina de Investigaciones en Bioquímica y Biología Molecular (SAIB). XVI Congreso Anual de la Asociación Civil de Microbiología General (SAMIGE); Argentina; 2021; 177-178
0327-9545
1667-5746
CONICET Digital
CONICET
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