Pet and stray dogs as reservoirs of antimicrobial-resistant Escherichia coli
- Autores
- Marchetti, Maria Laura; Buldain, Daniel Cornelio; Gortari Castillo, Lihuel; Buchamer, Andrea Veronica; Chirino Trejo, Manuel; Mestorino, Olga Nora
- Año de publicación
- 2021
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The close contact between dogs and humans creates the best bridge for interspecies transmission of antimicrobial-resistant bacteria. The surveillance of its resistance including the detection of extended-spectrum beta-lactamases (ESBLs) in Escherichia coli as indicator bacteria is an important tool to control the use of antimicrobials. The aim of this research was to evaluate the E. coli resistance in strains by phenotypic methods, isolated from pet and stray dogs of La Plata city, Argentina. Faecal samples were collected using rectal swabs from 50 dogs with owners (home dogs = HD) and 50 homeless dogs (stray dogs = SD). They were cultured in 3 MacConkey agar plates, with and without antibiotics (ciprofloxacin and cefotaxime). 197 strains were isolated, of which only 95 strains were biochemically identified as E. coli, 46 strains were from HD, and 49 were from SD. Antimicrobial susceptibility was evaluated by the Kirby-Bauer disk diffusion method. The most prevalent resistance was for tetracycline, streptomycin, and ampicillin. In both groups, the level of resistance to 3rd generation cephalosporins was high, and there were multiresistant strains. There was a higher level of antimicrobial resistance in strains from SD compared to HD. There were 8% of strains suspected of being ESBLs among samples of HD and 36% of SD. One (2%) of the strains isolated from HD and 11 (22%) from SD were phenotypically confirmed as ESBL. Pets and stray dogs are a potential source of E. coli antibiotic resistance in Argentina; therefore, its surveillance must be guaranteed.
Fil: Marchetti, Maria Laura. Universidad Nacional de la Plata. Facultad de Cs.veterinarias. Laboratorio de Estudios Farmacologicos y Toxicologicos.; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Buldain, Daniel Cornelio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de la Plata. Facultad de Cs.veterinarias. Laboratorio de Estudios Farmacologicos y Toxicologicos.; Argentina
Fil: Gortari Castillo, Lihuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de la Plata. Facultad de Cs.veterinarias. Laboratorio de Estudios Farmacológicos y Toxicológicos; Argentina
Fil: Buchamer, Andrea Veronica. Universidad Nacional de la Plata. Facultad de Cs.veterinarias. Laboratorio de Estudios Farmacologicos y Toxicologicos.; Argentina
Fil: Chirino Trejo, Manuel. University of Saskatchewan; Canadá
Fil: Mestorino, Olga Nora. Universidad Nacional de la Plata. Facultad de Cs.veterinarias. Laboratorio de Estudios Farmacologicos y Toxicologicos.; Argentina - Materia
-
Resistance profile
Escherichia coli
ESBL
Pets - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by/2.5/ar/
- Repositorio
.jpg)
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/167190
Ver los metadatos del registro completo
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Pet and stray dogs as reservoirs of antimicrobial-resistant Escherichia coliMarchetti, Maria LauraBuldain, Daniel CornelioGortari Castillo, LihuelBuchamer, Andrea VeronicaChirino Trejo, ManuelMestorino, Olga NoraResistance profileEscherichia coliESBLPetshttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4The close contact between dogs and humans creates the best bridge for interspecies transmission of antimicrobial-resistant bacteria. The surveillance of its resistance including the detection of extended-spectrum beta-lactamases (ESBLs) in Escherichia coli as indicator bacteria is an important tool to control the use of antimicrobials. The aim of this research was to evaluate the E. coli resistance in strains by phenotypic methods, isolated from pet and stray dogs of La Plata city, Argentina. Faecal samples were collected using rectal swabs from 50 dogs with owners (home dogs = HD) and 50 homeless dogs (stray dogs = SD). They were cultured in 3 MacConkey agar plates, with and without antibiotics (ciprofloxacin and cefotaxime). 197 strains were isolated, of which only 95 strains were biochemically identified as E. coli, 46 strains were from HD, and 49 were from SD. Antimicrobial susceptibility was evaluated by the Kirby-Bauer disk diffusion method. The most prevalent resistance was for tetracycline, streptomycin, and ampicillin. In both groups, the level of resistance to 3rd generation cephalosporins was high, and there were multiresistant strains. There was a higher level of antimicrobial resistance in strains from SD compared to HD. There were 8% of strains suspected of being ESBLs among samples of HD and 36% of SD. One (2%) of the strains isolated from HD and 11 (22%) from SD were phenotypically confirmed as ESBL. Pets and stray dogs are a potential source of E. coli antibiotic resistance in Argentina; therefore, its surveillance must be guaranteed.Fil: Marchetti, Maria Laura. Universidad Nacional de la Plata. Facultad de Cs.veterinarias. Laboratorio de Estudios Farmacologicos y Toxicologicos.; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: Buldain, Daniel Cornelio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de la Plata. Facultad de Cs.veterinarias. Laboratorio de Estudios Farmacologicos y Toxicologicos.; ArgentinaFil: Gortari Castillo, Lihuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de la Plata. Facultad de Cs.veterinarias. Laboratorio de Estudios Farmacológicos y Toxicológicos; ArgentinaFil: Buchamer, Andrea Veronica. Universidad Nacional de la Plata. Facultad de Cs.veterinarias. Laboratorio de Estudios Farmacologicos y Toxicologicos.; ArgentinaFil: Chirino Trejo, Manuel. University of Saskatchewan; CanadáFil: Mestorino, Olga Nora. Universidad Nacional de la Plata. Facultad de Cs.veterinarias. Laboratorio de Estudios Farmacologicos y Toxicologicos.; ArgentinaHindawi Publishing Corporation2021-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/167190Marchetti, Maria Laura; Buldain, Daniel Cornelio; Gortari Castillo, Lihuel; Buchamer, Andrea Veronica; Chirino Trejo, Manuel; et al.; Pet and stray dogs as reservoirs of antimicrobial-resistant Escherichia coli; Hindawi Publishing Corporation; International Journal of Microbiology; 2021; 1-2021; 1-81687-918X1687-9198CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.hindawi.com/journals/ijmicro/2021/6664557/info:eu-repo/semantics/altIdentifier/doi/10.1155/2021/6664557info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:59:24Zoai:ri.conicet.gov.ar:11336/167190instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:59:24.638CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
| dc.title.none.fl_str_mv |
Pet and stray dogs as reservoirs of antimicrobial-resistant Escherichia coli |
| title |
Pet and stray dogs as reservoirs of antimicrobial-resistant Escherichia coli |
| spellingShingle |
Pet and stray dogs as reservoirs of antimicrobial-resistant Escherichia coli Marchetti, Maria Laura Resistance profile Escherichia coli ESBL Pets |
| title_short |
Pet and stray dogs as reservoirs of antimicrobial-resistant Escherichia coli |
| title_full |
Pet and stray dogs as reservoirs of antimicrobial-resistant Escherichia coli |
| title_fullStr |
Pet and stray dogs as reservoirs of antimicrobial-resistant Escherichia coli |
| title_full_unstemmed |
Pet and stray dogs as reservoirs of antimicrobial-resistant Escherichia coli |
| title_sort |
Pet and stray dogs as reservoirs of antimicrobial-resistant Escherichia coli |
| dc.creator.none.fl_str_mv |
Marchetti, Maria Laura Buldain, Daniel Cornelio Gortari Castillo, Lihuel Buchamer, Andrea Veronica Chirino Trejo, Manuel Mestorino, Olga Nora |
| author |
Marchetti, Maria Laura |
| author_facet |
Marchetti, Maria Laura Buldain, Daniel Cornelio Gortari Castillo, Lihuel Buchamer, Andrea Veronica Chirino Trejo, Manuel Mestorino, Olga Nora |
| author_role |
author |
| author2 |
Buldain, Daniel Cornelio Gortari Castillo, Lihuel Buchamer, Andrea Veronica Chirino Trejo, Manuel Mestorino, Olga Nora |
| author2_role |
author author author author author |
| dc.subject.none.fl_str_mv |
Resistance profile Escherichia coli ESBL Pets |
| topic |
Resistance profile Escherichia coli ESBL Pets |
| purl_subject.fl_str_mv |
https://purl.org/becyt/ford/4.3 https://purl.org/becyt/ford/4 |
| dc.description.none.fl_txt_mv |
The close contact between dogs and humans creates the best bridge for interspecies transmission of antimicrobial-resistant bacteria. The surveillance of its resistance including the detection of extended-spectrum beta-lactamases (ESBLs) in Escherichia coli as indicator bacteria is an important tool to control the use of antimicrobials. The aim of this research was to evaluate the E. coli resistance in strains by phenotypic methods, isolated from pet and stray dogs of La Plata city, Argentina. Faecal samples were collected using rectal swabs from 50 dogs with owners (home dogs = HD) and 50 homeless dogs (stray dogs = SD). They were cultured in 3 MacConkey agar plates, with and without antibiotics (ciprofloxacin and cefotaxime). 197 strains were isolated, of which only 95 strains were biochemically identified as E. coli, 46 strains were from HD, and 49 were from SD. Antimicrobial susceptibility was evaluated by the Kirby-Bauer disk diffusion method. The most prevalent resistance was for tetracycline, streptomycin, and ampicillin. In both groups, the level of resistance to 3rd generation cephalosporins was high, and there were multiresistant strains. There was a higher level of antimicrobial resistance in strains from SD compared to HD. There were 8% of strains suspected of being ESBLs among samples of HD and 36% of SD. One (2%) of the strains isolated from HD and 11 (22%) from SD were phenotypically confirmed as ESBL. Pets and stray dogs are a potential source of E. coli antibiotic resistance in Argentina; therefore, its surveillance must be guaranteed. Fil: Marchetti, Maria Laura. Universidad Nacional de la Plata. Facultad de Cs.veterinarias. Laboratorio de Estudios Farmacologicos y Toxicologicos.; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina Fil: Buldain, Daniel Cornelio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de la Plata. Facultad de Cs.veterinarias. Laboratorio de Estudios Farmacologicos y Toxicologicos.; Argentina Fil: Gortari Castillo, Lihuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de la Plata. Facultad de Cs.veterinarias. Laboratorio de Estudios Farmacológicos y Toxicológicos; Argentina Fil: Buchamer, Andrea Veronica. Universidad Nacional de la Plata. Facultad de Cs.veterinarias. Laboratorio de Estudios Farmacologicos y Toxicologicos.; Argentina Fil: Chirino Trejo, Manuel. University of Saskatchewan; Canadá Fil: Mestorino, Olga Nora. Universidad Nacional de la Plata. Facultad de Cs.veterinarias. Laboratorio de Estudios Farmacologicos y Toxicologicos.; Argentina |
| description |
The close contact between dogs and humans creates the best bridge for interspecies transmission of antimicrobial-resistant bacteria. The surveillance of its resistance including the detection of extended-spectrum beta-lactamases (ESBLs) in Escherichia coli as indicator bacteria is an important tool to control the use of antimicrobials. The aim of this research was to evaluate the E. coli resistance in strains by phenotypic methods, isolated from pet and stray dogs of La Plata city, Argentina. Faecal samples were collected using rectal swabs from 50 dogs with owners (home dogs = HD) and 50 homeless dogs (stray dogs = SD). They were cultured in 3 MacConkey agar plates, with and without antibiotics (ciprofloxacin and cefotaxime). 197 strains were isolated, of which only 95 strains were biochemically identified as E. coli, 46 strains were from HD, and 49 were from SD. Antimicrobial susceptibility was evaluated by the Kirby-Bauer disk diffusion method. The most prevalent resistance was for tetracycline, streptomycin, and ampicillin. In both groups, the level of resistance to 3rd generation cephalosporins was high, and there were multiresistant strains. There was a higher level of antimicrobial resistance in strains from SD compared to HD. There were 8% of strains suspected of being ESBLs among samples of HD and 36% of SD. One (2%) of the strains isolated from HD and 11 (22%) from SD were phenotypically confirmed as ESBL. Pets and stray dogs are a potential source of E. coli antibiotic resistance in Argentina; therefore, its surveillance must be guaranteed. |
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2021 |
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2021-01 |
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http://hdl.handle.net/11336/167190 Marchetti, Maria Laura; Buldain, Daniel Cornelio; Gortari Castillo, Lihuel; Buchamer, Andrea Veronica; Chirino Trejo, Manuel; et al.; Pet and stray dogs as reservoirs of antimicrobial-resistant Escherichia coli; Hindawi Publishing Corporation; International Journal of Microbiology; 2021; 1-2021; 1-8 1687-918X 1687-9198 CONICET Digital CONICET |
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http://hdl.handle.net/11336/167190 |
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Marchetti, Maria Laura; Buldain, Daniel Cornelio; Gortari Castillo, Lihuel; Buchamer, Andrea Veronica; Chirino Trejo, Manuel; et al.; Pet and stray dogs as reservoirs of antimicrobial-resistant Escherichia coli; Hindawi Publishing Corporation; International Journal of Microbiology; 2021; 1-2021; 1-8 1687-918X 1687-9198 CONICET Digital CONICET |
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eng |
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