Simulated gastrointestinal digestion of inclusion complexes based on ovalbumin nanoparticles and conjugated linoleic acid

Autores
Visentini, Flavia Fátima; Ferrado, Joana Belén; Perez, Adrián Alejandro; Santiago, Liliana Gabriela
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The objective of this work was to obtain and characterize conjugated linoleic acid (CLA) delivery systems based on ovalbumin nanoparticles (OVAn1 and OVAn2) and to study their behaviour under a standardised static in vitro digestion model. OVAn1 and OVAn2 were obtained by heat treatment (85 °C, 5 min, pH 11.35 and 7.5, respectively). OVAn1 and OVAn2 had hydrodynamic diameters of 24.63 ± 0.04 and 92.0 ± 0.2 nm, respectively, showing no significant differences in ζ potential values (p < 0.05) at pH 7.0. CLA nanocomplexes were examined in terms of size and ζ potential at pH 3.0 and 7.0, highlighting that binding of CLA caused an increase in size for OVA and both OVA nanoparticles. Morphological characterization was performed by confocal laser scanning microscopy (CSLM) finding that OVA and OVA nanoparticles had a circular shape. Also, the CLA encapsulation efficiency (EE) for OVA and OVA nanoparticles was studied, yielding EE values greater than 97% for all systems. Finally, systems were assayed for a standardized in vitro gastrointestinal digestion model considering gastric and intestinal steps. Macroscopic appearance, CSLM images and quantification of CLA retention by HPLC were evaluated after digestion. All the systems showed the formation of macroscopic aggregates both in gastric and intestinal phases, which generated a visible precipitate. In all systems, CSLM confirmed the presence of numerous undefined-form aggregates. Finally, high CLA retention (around 99%) was found for native protein and nanoparticles.
Fil: Visentini, Flavia Fátima. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Tecnología de los Alimentos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ferrado, Joana Belén. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Tecnología de los Alimentos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Perez, Adrián Alejandro. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Tecnología de los Alimentos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Santiago, Liliana Gabriela. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Tecnología de los Alimentos; Argentina
Materia
OVALBUMIN
NANOCOMPLEXES
CONJUGATED LINOLEIC ACID
IN VITRO GASTROINTESTINAL DIGESTION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/118696
Acceder
id CONICETDig_0e4395577d57d5442b9b333bf93789cf
oai_identifier_str oai:ri.conicet.gov.ar:11336/118696
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Simulated gastrointestinal digestion of inclusion complexes based on ovalbumin nanoparticles and conjugated linoleic acidVisentini, Flavia FátimaFerrado, Joana BelénPerez, Adrián AlejandroSantiago, Liliana GabrielaOVALBUMINNANOCOMPLEXESCONJUGATED LINOLEIC ACIDIN VITRO GASTROINTESTINAL DIGESTIONhttps://purl.org/becyt/ford/2.10https://purl.org/becyt/ford/2The objective of this work was to obtain and characterize conjugated linoleic acid (CLA) delivery systems based on ovalbumin nanoparticles (OVAn1 and OVAn2) and to study their behaviour under a standardised static in vitro digestion model. OVAn1 and OVAn2 were obtained by heat treatment (85 °C, 5 min, pH 11.35 and 7.5, respectively). OVAn1 and OVAn2 had hydrodynamic diameters of 24.63 ± 0.04 and 92.0 ± 0.2 nm, respectively, showing no significant differences in ζ potential values (p < 0.05) at pH 7.0. CLA nanocomplexes were examined in terms of size and ζ potential at pH 3.0 and 7.0, highlighting that binding of CLA caused an increase in size for OVA and both OVA nanoparticles. Morphological characterization was performed by confocal laser scanning microscopy (CSLM) finding that OVA and OVA nanoparticles had a circular shape. Also, the CLA encapsulation efficiency (EE) for OVA and OVA nanoparticles was studied, yielding EE values greater than 97% for all systems. Finally, systems were assayed for a standardized in vitro gastrointestinal digestion model considering gastric and intestinal steps. Macroscopic appearance, CSLM images and quantification of CLA retention by HPLC were evaluated after digestion. All the systems showed the formation of macroscopic aggregates both in gastric and intestinal phases, which generated a visible precipitate. In all systems, CSLM confirmed the presence of numerous undefined-form aggregates. Finally, high CLA retention (around 99%) was found for native protein and nanoparticles.Fil: Visentini, Flavia Fátima. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Tecnología de los Alimentos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Ferrado, Joana Belén. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Tecnología de los Alimentos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Perez, Adrián Alejandro. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Tecnología de los Alimentos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Santiago, Liliana Gabriela. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Tecnología de los Alimentos; ArgentinaRoyal Society of Chemistry2019-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/118696Visentini, Flavia Fátima; Ferrado, Joana Belén; Perez, Adrián Alejandro; Santiago, Liliana Gabriela; Simulated gastrointestinal digestion of inclusion complexes based on ovalbumin nanoparticles and conjugated linoleic acid; Royal Society of Chemistry ; Food & Function; 10; 5; 5-2019; 2630-26412042-650XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://xlink.rsc.org/?DOI=C8FO02416Binfo:eu-repo/semantics/altIdentifier/doi/10.1039/c8fo02416binfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:09:36Zoai:ri.conicet.gov.ar:11336/118696instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:09:36.345CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Simulated gastrointestinal digestion of inclusion complexes based on ovalbumin nanoparticles and conjugated linoleic acid
title Simulated gastrointestinal digestion of inclusion complexes based on ovalbumin nanoparticles and conjugated linoleic acid
spellingShingle Simulated gastrointestinal digestion of inclusion complexes based on ovalbumin nanoparticles and conjugated linoleic acid
Visentini, Flavia Fátima
OVALBUMIN
NANOCOMPLEXES
CONJUGATED LINOLEIC ACID
IN VITRO GASTROINTESTINAL DIGESTION
title_short Simulated gastrointestinal digestion of inclusion complexes based on ovalbumin nanoparticles and conjugated linoleic acid
title_full Simulated gastrointestinal digestion of inclusion complexes based on ovalbumin nanoparticles and conjugated linoleic acid
title_fullStr Simulated gastrointestinal digestion of inclusion complexes based on ovalbumin nanoparticles and conjugated linoleic acid
title_full_unstemmed Simulated gastrointestinal digestion of inclusion complexes based on ovalbumin nanoparticles and conjugated linoleic acid
title_sort Simulated gastrointestinal digestion of inclusion complexes based on ovalbumin nanoparticles and conjugated linoleic acid
dc.creator.none.fl_str_mv Visentini, Flavia Fátima
Ferrado, Joana Belén
Perez, Adrián Alejandro
Santiago, Liliana Gabriela
author Visentini, Flavia Fátima
author_facet Visentini, Flavia Fátima
Ferrado, Joana Belén
Perez, Adrián Alejandro
Santiago, Liliana Gabriela
author_role author
author2 Ferrado, Joana Belén
Perez, Adrián Alejandro
Santiago, Liliana Gabriela
author2_role author
author
author
dc.subject.none.fl_str_mv OVALBUMIN
NANOCOMPLEXES
CONJUGATED LINOLEIC ACID
IN VITRO GASTROINTESTINAL DIGESTION
topic OVALBUMIN
NANOCOMPLEXES
CONJUGATED LINOLEIC ACID
IN VITRO GASTROINTESTINAL DIGESTION
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.10
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv The objective of this work was to obtain and characterize conjugated linoleic acid (CLA) delivery systems based on ovalbumin nanoparticles (OVAn1 and OVAn2) and to study their behaviour under a standardised static in vitro digestion model. OVAn1 and OVAn2 were obtained by heat treatment (85 °C, 5 min, pH 11.35 and 7.5, respectively). OVAn1 and OVAn2 had hydrodynamic diameters of 24.63 ± 0.04 and 92.0 ± 0.2 nm, respectively, showing no significant differences in ζ potential values (p < 0.05) at pH 7.0. CLA nanocomplexes were examined in terms of size and ζ potential at pH 3.0 and 7.0, highlighting that binding of CLA caused an increase in size for OVA and both OVA nanoparticles. Morphological characterization was performed by confocal laser scanning microscopy (CSLM) finding that OVA and OVA nanoparticles had a circular shape. Also, the CLA encapsulation efficiency (EE) for OVA and OVA nanoparticles was studied, yielding EE values greater than 97% for all systems. Finally, systems were assayed for a standardized in vitro gastrointestinal digestion model considering gastric and intestinal steps. Macroscopic appearance, CSLM images and quantification of CLA retention by HPLC were evaluated after digestion. All the systems showed the formation of macroscopic aggregates both in gastric and intestinal phases, which generated a visible precipitate. In all systems, CSLM confirmed the presence of numerous undefined-form aggregates. Finally, high CLA retention (around 99%) was found for native protein and nanoparticles.
Fil: Visentini, Flavia Fátima. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Tecnología de los Alimentos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ferrado, Joana Belén. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Tecnología de los Alimentos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Perez, Adrián Alejandro. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Tecnología de los Alimentos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Santiago, Liliana Gabriela. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Tecnología de los Alimentos; Argentina
description The objective of this work was to obtain and characterize conjugated linoleic acid (CLA) delivery systems based on ovalbumin nanoparticles (OVAn1 and OVAn2) and to study their behaviour under a standardised static in vitro digestion model. OVAn1 and OVAn2 were obtained by heat treatment (85 °C, 5 min, pH 11.35 and 7.5, respectively). OVAn1 and OVAn2 had hydrodynamic diameters of 24.63 ± 0.04 and 92.0 ± 0.2 nm, respectively, showing no significant differences in ζ potential values (p < 0.05) at pH 7.0. CLA nanocomplexes were examined in terms of size and ζ potential at pH 3.0 and 7.0, highlighting that binding of CLA caused an increase in size for OVA and both OVA nanoparticles. Morphological characterization was performed by confocal laser scanning microscopy (CSLM) finding that OVA and OVA nanoparticles had a circular shape. Also, the CLA encapsulation efficiency (EE) for OVA and OVA nanoparticles was studied, yielding EE values greater than 97% for all systems. Finally, systems were assayed for a standardized in vitro gastrointestinal digestion model considering gastric and intestinal steps. Macroscopic appearance, CSLM images and quantification of CLA retention by HPLC were evaluated after digestion. All the systems showed the formation of macroscopic aggregates both in gastric and intestinal phases, which generated a visible precipitate. In all systems, CSLM confirmed the presence of numerous undefined-form aggregates. Finally, high CLA retention (around 99%) was found for native protein and nanoparticles.
publishDate 2019
dc.date.none.fl_str_mv 2019-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/118696
Visentini, Flavia Fátima; Ferrado, Joana Belén; Perez, Adrián Alejandro; Santiago, Liliana Gabriela; Simulated gastrointestinal digestion of inclusion complexes based on ovalbumin nanoparticles and conjugated linoleic acid; Royal Society of Chemistry ; Food & Function; 10; 5; 5-2019; 2630-2641
2042-650X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/118696
identifier_str_mv Visentini, Flavia Fátima; Ferrado, Joana Belén; Perez, Adrián Alejandro; Santiago, Liliana Gabriela; Simulated gastrointestinal digestion of inclusion complexes based on ovalbumin nanoparticles and conjugated linoleic acid; Royal Society of Chemistry ; Food & Function; 10; 5; 5-2019; 2630-2641
2042-650X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://xlink.rsc.org/?DOI=C8FO02416B
info:eu-repo/semantics/altIdentifier/doi/10.1039/c8fo02416b
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Royal Society of Chemistry
publisher.none.fl_str_mv Royal Society of Chemistry
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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