Immunological status of isolated lymphoid follicles after intestinal transplantation

Autores
Meier, Dominik; Docena, Guillermo H.; Ramisch, D.; Toscanini, U.; Berardi, G.; Gondolesi, Gabriel Eduardo; Rumbo, Martín
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Intestinal transplantation (ITx) faces the challenge of grafting a high immunogenic organ, which is certainly one of the major obstacles for intestinal allograft acceptance. The allograft has to guarantee the proper functioning of the mucosal immune machinery under immunosuppressive conditions. Recently, it has been elucidated that isolated lymphoid follicles (ILFs) are an indispensable part of mucosal immunity to maintain IgA synthesis and consequently to control commensal microflora. No data about these follicular structures in the setting of ITx are available so far. Therefore, we addressed the question whether constitution, integrity and function of allograft ILFs are disturbed by immunosuppressive regimen. We compared allograft ILFs from terminal ileum of transplant patients with ILFs from nontransplant patients via flow cytometry, quantitative real-time polymerase chain reaction and immunohistochemistry. We found that host leukocytes rapidly repopulate allograft ILFs and that maintenance immunosuppressive regimen, tacrolimus and corticosteroids, does not affect their cellular integrity and function. However, allograft ILFs revealed a higher maturation state than control samples and IgA positive plasma cells were increased in number in allograft mucosa. Our results open the path for a better understanding of allograft mucosal immunity. This study shows that cellular integrity and function of isolated lymphoid follicles in the small bowel of transplanted patients are not affected by the immunosuppressive regimen, and that these lymphoid structures serve to better understand the mucosal immunity of the intestinal allograft.
Fil: Meier, Dominik. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Favaloro; Argentina
Fil: Docena, Guillermo H.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigaciones del Sistema Inmune; Argentina
Fil: Ramisch, D.. Fundación Favaloro; Argentina
Fil: Toscanini, U.. Fundación Favaloro; Argentina
Fil: Berardi, G.. Fundación Favaloro; Argentina
Fil: Gondolesi, Gabriel Eduardo. Fundación Favaloro; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Rumbo, Martín. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigaciones del Sistema Inmune; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Materia
BASIC (LABORATORY) RESEARCH/SCIENCE
IMMUNOBIOLOGY
INTESTINAL BIOLOGY
INTESTINE/MULTIVISCERAL TRANSPLANTATION
MUCOSAL IMMUNITY
TRANSLATIONAL RESEARCH/SCIENCE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/100110
Acceder
id CONICETDig_0e26fadf155371324ed62dfb3e429b9f
oai_identifier_str oai:ri.conicet.gov.ar:11336/100110
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Immunological status of isolated lymphoid follicles after intestinal transplantationMeier, DominikDocena, Guillermo H.Ramisch, D.Toscanini, U.Berardi, G.Gondolesi, Gabriel EduardoRumbo, MartínBASIC (LABORATORY) RESEARCH/SCIENCEIMMUNOBIOLOGYINTESTINAL BIOLOGYINTESTINE/MULTIVISCERAL TRANSPLANTATIONMUCOSAL IMMUNITYTRANSLATIONAL RESEARCH/SCIENCEhttps://purl.org/becyt/ford/3.2https://purl.org/becyt/ford/3Intestinal transplantation (ITx) faces the challenge of grafting a high immunogenic organ, which is certainly one of the major obstacles for intestinal allograft acceptance. The allograft has to guarantee the proper functioning of the mucosal immune machinery under immunosuppressive conditions. Recently, it has been elucidated that isolated lymphoid follicles (ILFs) are an indispensable part of mucosal immunity to maintain IgA synthesis and consequently to control commensal microflora. No data about these follicular structures in the setting of ITx are available so far. Therefore, we addressed the question whether constitution, integrity and function of allograft ILFs are disturbed by immunosuppressive regimen. We compared allograft ILFs from terminal ileum of transplant patients with ILFs from nontransplant patients via flow cytometry, quantitative real-time polymerase chain reaction and immunohistochemistry. We found that host leukocytes rapidly repopulate allograft ILFs and that maintenance immunosuppressive regimen, tacrolimus and corticosteroids, does not affect their cellular integrity and function. However, allograft ILFs revealed a higher maturation state than control samples and IgA positive plasma cells were increased in number in allograft mucosa. Our results open the path for a better understanding of allograft mucosal immunity. This study shows that cellular integrity and function of isolated lymphoid follicles in the small bowel of transplanted patients are not affected by the immunosuppressive regimen, and that these lymphoid structures serve to better understand the mucosal immunity of the intestinal allograft.Fil: Meier, Dominik. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Favaloro; ArgentinaFil: Docena, Guillermo H.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigaciones del Sistema Inmune; ArgentinaFil: Ramisch, D.. Fundación Favaloro; ArgentinaFil: Toscanini, U.. Fundación Favaloro; ArgentinaFil: Berardi, G.. Fundación Favaloro; ArgentinaFil: Gondolesi, Gabriel Eduardo. Fundación Favaloro; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Rumbo, Martín. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigaciones del Sistema Inmune; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaWiley Blackwell Publishing, Inc2014-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/100110Meier, Dominik; Docena, Guillermo H.; Ramisch, D.; Toscanini, U.; Berardi, G.; et al.; Immunological status of isolated lymphoid follicles after intestinal transplantation; Wiley Blackwell Publishing, Inc; American Journal of Transplantation; 14; 9; 9-2014; 2148-21581600-6135CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/full/10.1111/ajt.12797info:eu-repo/semantics/altIdentifier/doi/10.1111/ajt.12797info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:02:22Zoai:ri.conicet.gov.ar:11336/100110instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:02:22.565CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Immunological status of isolated lymphoid follicles after intestinal transplantation
title Immunological status of isolated lymphoid follicles after intestinal transplantation
spellingShingle Immunological status of isolated lymphoid follicles after intestinal transplantation
Meier, Dominik
BASIC (LABORATORY) RESEARCH/SCIENCE
IMMUNOBIOLOGY
INTESTINAL BIOLOGY
INTESTINE/MULTIVISCERAL TRANSPLANTATION
MUCOSAL IMMUNITY
TRANSLATIONAL RESEARCH/SCIENCE
title_short Immunological status of isolated lymphoid follicles after intestinal transplantation
title_full Immunological status of isolated lymphoid follicles after intestinal transplantation
title_fullStr Immunological status of isolated lymphoid follicles after intestinal transplantation
title_full_unstemmed Immunological status of isolated lymphoid follicles after intestinal transplantation
title_sort Immunological status of isolated lymphoid follicles after intestinal transplantation
dc.creator.none.fl_str_mv Meier, Dominik
Docena, Guillermo H.
Ramisch, D.
Toscanini, U.
Berardi, G.
Gondolesi, Gabriel Eduardo
Rumbo, Martín
author Meier, Dominik
author_facet Meier, Dominik
Docena, Guillermo H.
Ramisch, D.
Toscanini, U.
Berardi, G.
Gondolesi, Gabriel Eduardo
Rumbo, Martín
author_role author
author2 Docena, Guillermo H.
Ramisch, D.
Toscanini, U.
Berardi, G.
Gondolesi, Gabriel Eduardo
Rumbo, Martín
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv BASIC (LABORATORY) RESEARCH/SCIENCE
IMMUNOBIOLOGY
INTESTINAL BIOLOGY
INTESTINE/MULTIVISCERAL TRANSPLANTATION
MUCOSAL IMMUNITY
TRANSLATIONAL RESEARCH/SCIENCE
topic BASIC (LABORATORY) RESEARCH/SCIENCE
IMMUNOBIOLOGY
INTESTINAL BIOLOGY
INTESTINE/MULTIVISCERAL TRANSPLANTATION
MUCOSAL IMMUNITY
TRANSLATIONAL RESEARCH/SCIENCE
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.2
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Intestinal transplantation (ITx) faces the challenge of grafting a high immunogenic organ, which is certainly one of the major obstacles for intestinal allograft acceptance. The allograft has to guarantee the proper functioning of the mucosal immune machinery under immunosuppressive conditions. Recently, it has been elucidated that isolated lymphoid follicles (ILFs) are an indispensable part of mucosal immunity to maintain IgA synthesis and consequently to control commensal microflora. No data about these follicular structures in the setting of ITx are available so far. Therefore, we addressed the question whether constitution, integrity and function of allograft ILFs are disturbed by immunosuppressive regimen. We compared allograft ILFs from terminal ileum of transplant patients with ILFs from nontransplant patients via flow cytometry, quantitative real-time polymerase chain reaction and immunohistochemistry. We found that host leukocytes rapidly repopulate allograft ILFs and that maintenance immunosuppressive regimen, tacrolimus and corticosteroids, does not affect their cellular integrity and function. However, allograft ILFs revealed a higher maturation state than control samples and IgA positive plasma cells were increased in number in allograft mucosa. Our results open the path for a better understanding of allograft mucosal immunity. This study shows that cellular integrity and function of isolated lymphoid follicles in the small bowel of transplanted patients are not affected by the immunosuppressive regimen, and that these lymphoid structures serve to better understand the mucosal immunity of the intestinal allograft.
Fil: Meier, Dominik. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Favaloro; Argentina
Fil: Docena, Guillermo H.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigaciones del Sistema Inmune; Argentina
Fil: Ramisch, D.. Fundación Favaloro; Argentina
Fil: Toscanini, U.. Fundación Favaloro; Argentina
Fil: Berardi, G.. Fundación Favaloro; Argentina
Fil: Gondolesi, Gabriel Eduardo. Fundación Favaloro; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Rumbo, Martín. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigaciones del Sistema Inmune; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
description Intestinal transplantation (ITx) faces the challenge of grafting a high immunogenic organ, which is certainly one of the major obstacles for intestinal allograft acceptance. The allograft has to guarantee the proper functioning of the mucosal immune machinery under immunosuppressive conditions. Recently, it has been elucidated that isolated lymphoid follicles (ILFs) are an indispensable part of mucosal immunity to maintain IgA synthesis and consequently to control commensal microflora. No data about these follicular structures in the setting of ITx are available so far. Therefore, we addressed the question whether constitution, integrity and function of allograft ILFs are disturbed by immunosuppressive regimen. We compared allograft ILFs from terminal ileum of transplant patients with ILFs from nontransplant patients via flow cytometry, quantitative real-time polymerase chain reaction and immunohistochemistry. We found that host leukocytes rapidly repopulate allograft ILFs and that maintenance immunosuppressive regimen, tacrolimus and corticosteroids, does not affect their cellular integrity and function. However, allograft ILFs revealed a higher maturation state than control samples and IgA positive plasma cells were increased in number in allograft mucosa. Our results open the path for a better understanding of allograft mucosal immunity. This study shows that cellular integrity and function of isolated lymphoid follicles in the small bowel of transplanted patients are not affected by the immunosuppressive regimen, and that these lymphoid structures serve to better understand the mucosal immunity of the intestinal allograft.
publishDate 2014
dc.date.none.fl_str_mv 2014-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/100110
Meier, Dominik; Docena, Guillermo H.; Ramisch, D.; Toscanini, U.; Berardi, G.; et al.; Immunological status of isolated lymphoid follicles after intestinal transplantation; Wiley Blackwell Publishing, Inc; American Journal of Transplantation; 14; 9; 9-2014; 2148-2158
1600-6135
CONICET Digital
CONICET
url http://hdl.handle.net/11336/100110
identifier_str_mv Meier, Dominik; Docena, Guillermo H.; Ramisch, D.; Toscanini, U.; Berardi, G.; et al.; Immunological status of isolated lymphoid follicles after intestinal transplantation; Wiley Blackwell Publishing, Inc; American Journal of Transplantation; 14; 9; 9-2014; 2148-2158
1600-6135
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/full/10.1111/ajt.12797
info:eu-repo/semantics/altIdentifier/doi/10.1111/ajt.12797
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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