Clinical parameters, postmortem analysis and estimation of lethal dose in victims of a massive intoxication with diethylene glycol
- Autores
- Ferrari, Luis Alberto; Giannuzzi, Leda
- Año de publicación
- 2005
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- This work analyzes a massive intoxication that occurred in 1992 in Argentina as a result of the use of propolis syrup as a popular upper respiratory infection medicinal agent. The intoxicating agent was diethylene glycol (DEG), which caused metabolic acidosis, anuria, renal failure and death in 15 out of the 29 studied victims. DEG poisoning cases were classified in three groups according to survival time: Group 1?patients that survived up to 3 days; Group 2?patients that survived between 4 and 5 days; Group 3?patients that survived between 6 and 21 days. Patients from Group 1 showed the highest values of anion gap, the lowest measures of base excess (BE) and more severe clinical manifestations. Correlation between pH and BE was r2 = 0.68, 0.99 and 0.55 for Groups 1, 2 and 3, respectively. A methanolic extraction was performed on the fatal victims? viscera and blood, with subsequent concentration and purification. The semi-crystalline fraction obtained retained DEG by means of codissolution and adsorption as demonstrated by thin lay chromatography/flame ionisation detection (TLC/FID). In 3 out of the 15 fatal cases (from Group 1), DEG was isolated from viscera and blood (femoral venous), between 48 and 72 h post ingestion. The concentration relation (DEG)viscera/(DEG)blood ranged from 1.45 to 1.55 with a coefficient correlation r2 = 0.96 (n = 3). In the other victims, DEG could not be detected. The reason for this could be the long survival period of the victims after their ingestion of the syrup. Additionally, putrefying mechanisms could have been operating. Samples of the propolis syrup of each victim were studied by means of nuclear magnetic resonance (NMR) and quantified by gas chromatography/flame ionisation detection (GC/FID). Results showed that syrup samples contained 65.0% (w/v) of diethylene glycol (DEG) and 32.0% (w/v) of propylene glycol (PG). A good correlation between the amount of DEG ingested and the anion gap (r2 = 0.63) for the 15 victims studied could be observed. The lethal dose for human beings estimated in this work ranged from 0.014 to 0.170 mg DEG/kg body weight. This is a lower lethal dose than reported in a separate incident in Haiti. These results may contribute to the understanding of DEG?s metabolic pathway and provides data from lethal doses in humans.
Fil: Ferrari, Luis Alberto. Universidad de Morón. Facultad de Ciencias Exactas, Químicas y Naturales; Argentina
Fil: Giannuzzi, Leda. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina - Materia
-
DIETHYLENE GLYCOL
POSTMORTEM TISSUE
CLINICAL PARAMETERS
PROPOLIS SYRUP - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/158796
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Clinical parameters, postmortem analysis and estimation of lethal dose in victims of a massive intoxication with diethylene glycolFerrari, Luis AlbertoGiannuzzi, LedaDIETHYLENE GLYCOLPOSTMORTEM TISSUECLINICAL PARAMETERSPROPOLIS SYRUPhttps://purl.org/becyt/ford/2.11https://purl.org/becyt/ford/2This work analyzes a massive intoxication that occurred in 1992 in Argentina as a result of the use of propolis syrup as a popular upper respiratory infection medicinal agent. The intoxicating agent was diethylene glycol (DEG), which caused metabolic acidosis, anuria, renal failure and death in 15 out of the 29 studied victims. DEG poisoning cases were classified in three groups according to survival time: Group 1?patients that survived up to 3 days; Group 2?patients that survived between 4 and 5 days; Group 3?patients that survived between 6 and 21 days. Patients from Group 1 showed the highest values of anion gap, the lowest measures of base excess (BE) and more severe clinical manifestations. Correlation between pH and BE was r2 = 0.68, 0.99 and 0.55 for Groups 1, 2 and 3, respectively. A methanolic extraction was performed on the fatal victims? viscera and blood, with subsequent concentration and purification. The semi-crystalline fraction obtained retained DEG by means of codissolution and adsorption as demonstrated by thin lay chromatography/flame ionisation detection (TLC/FID). In 3 out of the 15 fatal cases (from Group 1), DEG was isolated from viscera and blood (femoral venous), between 48 and 72 h post ingestion. The concentration relation (DEG)viscera/(DEG)blood ranged from 1.45 to 1.55 with a coefficient correlation r2 = 0.96 (n = 3). In the other victims, DEG could not be detected. The reason for this could be the long survival period of the victims after their ingestion of the syrup. Additionally, putrefying mechanisms could have been operating. Samples of the propolis syrup of each victim were studied by means of nuclear magnetic resonance (NMR) and quantified by gas chromatography/flame ionisation detection (GC/FID). Results showed that syrup samples contained 65.0% (w/v) of diethylene glycol (DEG) and 32.0% (w/v) of propylene glycol (PG). A good correlation between the amount of DEG ingested and the anion gap (r2 = 0.63) for the 15 victims studied could be observed. The lethal dose for human beings estimated in this work ranged from 0.014 to 0.170 mg DEG/kg body weight. This is a lower lethal dose than reported in a separate incident in Haiti. These results may contribute to the understanding of DEG?s metabolic pathway and provides data from lethal doses in humans.Fil: Ferrari, Luis Alberto. Universidad de Morón. Facultad de Ciencias Exactas, Químicas y Naturales; ArgentinaFil: Giannuzzi, Leda. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; ArgentinaElsevier Ireland2005-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/158796Ferrari, Luis Alberto; Giannuzzi, Leda; Clinical parameters, postmortem analysis and estimation of lethal dose in victims of a massive intoxication with diethylene glycol; Elsevier Ireland; Forensic Science International; 153; 1; 10-2005; 45-510379-07381872-6283CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0379073805002070info:eu-repo/semantics/altIdentifier/doi/10.1016/j.forsciint.2005.04.038info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:45:00Zoai:ri.conicet.gov.ar:11336/158796instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:45:00.987CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Clinical parameters, postmortem analysis and estimation of lethal dose in victims of a massive intoxication with diethylene glycol |
title |
Clinical parameters, postmortem analysis and estimation of lethal dose in victims of a massive intoxication with diethylene glycol |
spellingShingle |
Clinical parameters, postmortem analysis and estimation of lethal dose in victims of a massive intoxication with diethylene glycol Ferrari, Luis Alberto DIETHYLENE GLYCOL POSTMORTEM TISSUE CLINICAL PARAMETERS PROPOLIS SYRUP |
title_short |
Clinical parameters, postmortem analysis and estimation of lethal dose in victims of a massive intoxication with diethylene glycol |
title_full |
Clinical parameters, postmortem analysis and estimation of lethal dose in victims of a massive intoxication with diethylene glycol |
title_fullStr |
Clinical parameters, postmortem analysis and estimation of lethal dose in victims of a massive intoxication with diethylene glycol |
title_full_unstemmed |
Clinical parameters, postmortem analysis and estimation of lethal dose in victims of a massive intoxication with diethylene glycol |
title_sort |
Clinical parameters, postmortem analysis and estimation of lethal dose in victims of a massive intoxication with diethylene glycol |
dc.creator.none.fl_str_mv |
Ferrari, Luis Alberto Giannuzzi, Leda |
author |
Ferrari, Luis Alberto |
author_facet |
Ferrari, Luis Alberto Giannuzzi, Leda |
author_role |
author |
author2 |
Giannuzzi, Leda |
author2_role |
author |
dc.subject.none.fl_str_mv |
DIETHYLENE GLYCOL POSTMORTEM TISSUE CLINICAL PARAMETERS PROPOLIS SYRUP |
topic |
DIETHYLENE GLYCOL POSTMORTEM TISSUE CLINICAL PARAMETERS PROPOLIS SYRUP |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/2.11 https://purl.org/becyt/ford/2 |
dc.description.none.fl_txt_mv |
This work analyzes a massive intoxication that occurred in 1992 in Argentina as a result of the use of propolis syrup as a popular upper respiratory infection medicinal agent. The intoxicating agent was diethylene glycol (DEG), which caused metabolic acidosis, anuria, renal failure and death in 15 out of the 29 studied victims. DEG poisoning cases were classified in three groups according to survival time: Group 1?patients that survived up to 3 days; Group 2?patients that survived between 4 and 5 days; Group 3?patients that survived between 6 and 21 days. Patients from Group 1 showed the highest values of anion gap, the lowest measures of base excess (BE) and more severe clinical manifestations. Correlation between pH and BE was r2 = 0.68, 0.99 and 0.55 for Groups 1, 2 and 3, respectively. A methanolic extraction was performed on the fatal victims? viscera and blood, with subsequent concentration and purification. The semi-crystalline fraction obtained retained DEG by means of codissolution and adsorption as demonstrated by thin lay chromatography/flame ionisation detection (TLC/FID). In 3 out of the 15 fatal cases (from Group 1), DEG was isolated from viscera and blood (femoral venous), between 48 and 72 h post ingestion. The concentration relation (DEG)viscera/(DEG)blood ranged from 1.45 to 1.55 with a coefficient correlation r2 = 0.96 (n = 3). In the other victims, DEG could not be detected. The reason for this could be the long survival period of the victims after their ingestion of the syrup. Additionally, putrefying mechanisms could have been operating. Samples of the propolis syrup of each victim were studied by means of nuclear magnetic resonance (NMR) and quantified by gas chromatography/flame ionisation detection (GC/FID). Results showed that syrup samples contained 65.0% (w/v) of diethylene glycol (DEG) and 32.0% (w/v) of propylene glycol (PG). A good correlation between the amount of DEG ingested and the anion gap (r2 = 0.63) for the 15 victims studied could be observed. The lethal dose for human beings estimated in this work ranged from 0.014 to 0.170 mg DEG/kg body weight. This is a lower lethal dose than reported in a separate incident in Haiti. These results may contribute to the understanding of DEG?s metabolic pathway and provides data from lethal doses in humans. Fil: Ferrari, Luis Alberto. Universidad de Morón. Facultad de Ciencias Exactas, Químicas y Naturales; Argentina Fil: Giannuzzi, Leda. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina |
description |
This work analyzes a massive intoxication that occurred in 1992 in Argentina as a result of the use of propolis syrup as a popular upper respiratory infection medicinal agent. The intoxicating agent was diethylene glycol (DEG), which caused metabolic acidosis, anuria, renal failure and death in 15 out of the 29 studied victims. DEG poisoning cases were classified in three groups according to survival time: Group 1?patients that survived up to 3 days; Group 2?patients that survived between 4 and 5 days; Group 3?patients that survived between 6 and 21 days. Patients from Group 1 showed the highest values of anion gap, the lowest measures of base excess (BE) and more severe clinical manifestations. Correlation between pH and BE was r2 = 0.68, 0.99 and 0.55 for Groups 1, 2 and 3, respectively. A methanolic extraction was performed on the fatal victims? viscera and blood, with subsequent concentration and purification. The semi-crystalline fraction obtained retained DEG by means of codissolution and adsorption as demonstrated by thin lay chromatography/flame ionisation detection (TLC/FID). In 3 out of the 15 fatal cases (from Group 1), DEG was isolated from viscera and blood (femoral venous), between 48 and 72 h post ingestion. The concentration relation (DEG)viscera/(DEG)blood ranged from 1.45 to 1.55 with a coefficient correlation r2 = 0.96 (n = 3). In the other victims, DEG could not be detected. The reason for this could be the long survival period of the victims after their ingestion of the syrup. Additionally, putrefying mechanisms could have been operating. Samples of the propolis syrup of each victim were studied by means of nuclear magnetic resonance (NMR) and quantified by gas chromatography/flame ionisation detection (GC/FID). Results showed that syrup samples contained 65.0% (w/v) of diethylene glycol (DEG) and 32.0% (w/v) of propylene glycol (PG). A good correlation between the amount of DEG ingested and the anion gap (r2 = 0.63) for the 15 victims studied could be observed. The lethal dose for human beings estimated in this work ranged from 0.014 to 0.170 mg DEG/kg body weight. This is a lower lethal dose than reported in a separate incident in Haiti. These results may contribute to the understanding of DEG?s metabolic pathway and provides data from lethal doses in humans. |
publishDate |
2005 |
dc.date.none.fl_str_mv |
2005-10 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/158796 Ferrari, Luis Alberto; Giannuzzi, Leda; Clinical parameters, postmortem analysis and estimation of lethal dose in victims of a massive intoxication with diethylene glycol; Elsevier Ireland; Forensic Science International; 153; 1; 10-2005; 45-51 0379-0738 1872-6283 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/158796 |
identifier_str_mv |
Ferrari, Luis Alberto; Giannuzzi, Leda; Clinical parameters, postmortem analysis and estimation of lethal dose in victims of a massive intoxication with diethylene glycol; Elsevier Ireland; Forensic Science International; 153; 1; 10-2005; 45-51 0379-0738 1872-6283 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0379073805002070 info:eu-repo/semantics/altIdentifier/doi/10.1016/j.forsciint.2005.04.038 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier Ireland |
publisher.none.fl_str_mv |
Elsevier Ireland |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844614489001951232 |
score |
13.070432 |