Effect of L. casei CRL 431 and its cellular wall on the thymus at different ages in a mice model

Autores
Balcells, Maria Florencia; Novotny Núñez, Ivanna; Perdigon, Gabriela del Valle; Maldonado Galdeano, María Carolina
Año de publicación
2018
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
The thymus is a primary lymphoid organ, where ontogeny and maturation of T lymphocytes occurs, however, it begins to regress with time.Aim: to study the effect of L. casei 431 and/or its cell wall in the thymus at different ages.BALB/c mice were divided into groups according to age: 21, 28, 45, 90 and 180 days(d), which were subdivided according to the supplement they received from the first day: Normal control (NC) who received the conventional balanced diet and water ad libitum, Lactobacillus casei (Lc) that received the conventional balanced diet and suspension of L. casei 431. Mice were sacrificed at the corresponding age. Initial and final weight of the animal, weight of the thymus was taken. The samples were: large intestine for microbiota analysis, serum (S), small intestinal fluid (IF) for cytokine analysis by ELISA (IFNγ, IL-6, IL-10, IL-12 p70, TNFα, IL-3) we also determined cytokines in the in vitro culture supernatant of thymus(T) stimulated with the bacterium complete (B) or its cell wall (W). T population in thymus, mesenteric lymph nodes (MLN) was analyzed by flow cytometry. Results: oral supplementation increased body weight in all groups. Body weight / thymus ratio decreased at 28, 45 and 90d in Lc. Supplementation showed decreased population of enterobacteria and increased the population of lactobacilli. We did not observe significant differences between NC and LC in the total anaerobic population.Cytokines in cell culture supernatant showed a decrease in IFNγ and TNFα, increased values of IL-10, IL-12 and IL-3, and we observed tendency to normalize IL-6 values of TB in Lc. The immune cells in Lc group in Timo, at 45d showed decrease in CD4+ populations, and significantly increased at 90d respect to NC, while we observed in NC CD4+/CD8+ population a significant increase at 90d (p <0.05) respect to LC (90d CD4+/CD8+: NC = 80.72 ± 0.32, LC = 0.45 ± 0.06).Oral supplementation and in vitro stimulation with complete bacteria and/or its cell wall produced effects in the thymus at different periods of time in mice.
Fil: Balcells, Maria Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Novotny Núñez, Ivanna. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Perdigon, Gabriela del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Maldonado Galdeano, María Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
LXIII Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXVI Reunión Anual de la Sociedad Argentina de Inmunología, Reunión Anual de la Sociedad Argentina de Fisiología
Mar del Plata
Argentina
Sociedad Argentina de Investigación Clínica
Sociedad Argentina de Inmunología
Sociedad Argentina de Fisiología
Materia
THYMUS
IMMUNOSENESCENCE
T LYMPHOCYTES
PROBIOTICS
CELL WALL
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/215766
Acceder
id CONICETDig_0d8389cfcf22dc68b2dd61627618adae
oai_identifier_str oai:ri.conicet.gov.ar:11336/215766
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Effect of L. casei CRL 431 and its cellular wall on the thymus at different ages in a mice modelBalcells, Maria FlorenciaNovotny Núñez, IvannaPerdigon, Gabriela del ValleMaldonado Galdeano, María CarolinaTHYMUSIMMUNOSENESCENCET LYMPHOCYTESPROBIOTICSCELL WALLhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The thymus is a primary lymphoid organ, where ontogeny and maturation of T lymphocytes occurs, however, it begins to regress with time.Aim: to study the effect of L. casei 431 and/or its cell wall in the thymus at different ages.BALB/c mice were divided into groups according to age: 21, 28, 45, 90 and 180 days(d), which were subdivided according to the supplement they received from the first day: Normal control (NC) who received the conventional balanced diet and water ad libitum, Lactobacillus casei (Lc) that received the conventional balanced diet and suspension of L. casei 431. Mice were sacrificed at the corresponding age. Initial and final weight of the animal, weight of the thymus was taken. The samples were: large intestine for microbiota analysis, serum (S), small intestinal fluid (IF) for cytokine analysis by ELISA (IFNγ, IL-6, IL-10, IL-12 p70, TNFα, IL-3) we also determined cytokines in the in vitro culture supernatant of thymus(T) stimulated with the bacterium complete (B) or its cell wall (W). T population in thymus, mesenteric lymph nodes (MLN) was analyzed by flow cytometry. Results: oral supplementation increased body weight in all groups. Body weight / thymus ratio decreased at 28, 45 and 90d in Lc. Supplementation showed decreased population of enterobacteria and increased the population of lactobacilli. We did not observe significant differences between NC and LC in the total anaerobic population.Cytokines in cell culture supernatant showed a decrease in IFNγ and TNFα, increased values of IL-10, IL-12 and IL-3, and we observed tendency to normalize IL-6 values of TB in Lc. The immune cells in Lc group in Timo, at 45d showed decrease in CD4+ populations, and significantly increased at 90d respect to NC, while we observed in NC CD4+/CD8+ population a significant increase at 90d (p <0.05) respect to LC (90d CD4+/CD8+: NC = 80.72 ± 0.32, LC = 0.45 ± 0.06).Oral supplementation and in vitro stimulation with complete bacteria and/or its cell wall produced effects in the thymus at different periods of time in mice.Fil: Balcells, Maria Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Novotny Núñez, Ivanna. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Perdigon, Gabriela del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Maldonado Galdeano, María Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaLXIII Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXVI Reunión Anual de la Sociedad Argentina de Inmunología, Reunión Anual de la Sociedad Argentina de FisiologíaMar del PlataArgentinaSociedad Argentina de Investigación ClínicaSociedad Argentina de InmunologíaSociedad Argentina de FisiologíaFundación Revista Medicina2018info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/215766Effect of L. casei CRL 431 and its cellular wall on the thymus at different ages in a mice model; LXIII Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXVI Reunión Anual de la Sociedad Argentina de Inmunología, Reunión Anual de la Sociedad Argentina de Fisiología; Mar del Plata; Argentina; 2018; 234-2340025-76801669-9106CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.medicinabuenosaires.com/revistas/vol78-18/s3/vol78supIII.pdfInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:43:53Zoai:ri.conicet.gov.ar:11336/215766instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:43:53.694CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effect of L. casei CRL 431 and its cellular wall on the thymus at different ages in a mice model
title Effect of L. casei CRL 431 and its cellular wall on the thymus at different ages in a mice model
spellingShingle Effect of L. casei CRL 431 and its cellular wall on the thymus at different ages in a mice model
Balcells, Maria Florencia
THYMUS
IMMUNOSENESCENCE
T LYMPHOCYTES
PROBIOTICS
CELL WALL
title_short Effect of L. casei CRL 431 and its cellular wall on the thymus at different ages in a mice model
title_full Effect of L. casei CRL 431 and its cellular wall on the thymus at different ages in a mice model
title_fullStr Effect of L. casei CRL 431 and its cellular wall on the thymus at different ages in a mice model
title_full_unstemmed Effect of L. casei CRL 431 and its cellular wall on the thymus at different ages in a mice model
title_sort Effect of L. casei CRL 431 and its cellular wall on the thymus at different ages in a mice model
dc.creator.none.fl_str_mv Balcells, Maria Florencia
Novotny Núñez, Ivanna
Perdigon, Gabriela del Valle
Maldonado Galdeano, María Carolina
author Balcells, Maria Florencia
author_facet Balcells, Maria Florencia
Novotny Núñez, Ivanna
Perdigon, Gabriela del Valle
Maldonado Galdeano, María Carolina
author_role author
author2 Novotny Núñez, Ivanna
Perdigon, Gabriela del Valle
Maldonado Galdeano, María Carolina
author2_role author
author
author
dc.subject.none.fl_str_mv THYMUS
IMMUNOSENESCENCE
T LYMPHOCYTES
PROBIOTICS
CELL WALL
topic THYMUS
IMMUNOSENESCENCE
T LYMPHOCYTES
PROBIOTICS
CELL WALL
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The thymus is a primary lymphoid organ, where ontogeny and maturation of T lymphocytes occurs, however, it begins to regress with time.Aim: to study the effect of L. casei 431 and/or its cell wall in the thymus at different ages.BALB/c mice were divided into groups according to age: 21, 28, 45, 90 and 180 days(d), which were subdivided according to the supplement they received from the first day: Normal control (NC) who received the conventional balanced diet and water ad libitum, Lactobacillus casei (Lc) that received the conventional balanced diet and suspension of L. casei 431. Mice were sacrificed at the corresponding age. Initial and final weight of the animal, weight of the thymus was taken. The samples were: large intestine for microbiota analysis, serum (S), small intestinal fluid (IF) for cytokine analysis by ELISA (IFNγ, IL-6, IL-10, IL-12 p70, TNFα, IL-3) we also determined cytokines in the in vitro culture supernatant of thymus(T) stimulated with the bacterium complete (B) or its cell wall (W). T population in thymus, mesenteric lymph nodes (MLN) was analyzed by flow cytometry. Results: oral supplementation increased body weight in all groups. Body weight / thymus ratio decreased at 28, 45 and 90d in Lc. Supplementation showed decreased population of enterobacteria and increased the population of lactobacilli. We did not observe significant differences between NC and LC in the total anaerobic population.Cytokines in cell culture supernatant showed a decrease in IFNγ and TNFα, increased values of IL-10, IL-12 and IL-3, and we observed tendency to normalize IL-6 values of TB in Lc. The immune cells in Lc group in Timo, at 45d showed decrease in CD4+ populations, and significantly increased at 90d respect to NC, while we observed in NC CD4+/CD8+ population a significant increase at 90d (p <0.05) respect to LC (90d CD4+/CD8+: NC = 80.72 ± 0.32, LC = 0.45 ± 0.06).Oral supplementation and in vitro stimulation with complete bacteria and/or its cell wall produced effects in the thymus at different periods of time in mice.
Fil: Balcells, Maria Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Novotny Núñez, Ivanna. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Perdigon, Gabriela del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Maldonado Galdeano, María Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
LXIII Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXVI Reunión Anual de la Sociedad Argentina de Inmunología, Reunión Anual de la Sociedad Argentina de Fisiología
Mar del Plata
Argentina
Sociedad Argentina de Investigación Clínica
Sociedad Argentina de Inmunología
Sociedad Argentina de Fisiología
description The thymus is a primary lymphoid organ, where ontogeny and maturation of T lymphocytes occurs, however, it begins to regress with time.Aim: to study the effect of L. casei 431 and/or its cell wall in the thymus at different ages.BALB/c mice were divided into groups according to age: 21, 28, 45, 90 and 180 days(d), which were subdivided according to the supplement they received from the first day: Normal control (NC) who received the conventional balanced diet and water ad libitum, Lactobacillus casei (Lc) that received the conventional balanced diet and suspension of L. casei 431. Mice were sacrificed at the corresponding age. Initial and final weight of the animal, weight of the thymus was taken. The samples were: large intestine for microbiota analysis, serum (S), small intestinal fluid (IF) for cytokine analysis by ELISA (IFNγ, IL-6, IL-10, IL-12 p70, TNFα, IL-3) we also determined cytokines in the in vitro culture supernatant of thymus(T) stimulated with the bacterium complete (B) or its cell wall (W). T population in thymus, mesenteric lymph nodes (MLN) was analyzed by flow cytometry. Results: oral supplementation increased body weight in all groups. Body weight / thymus ratio decreased at 28, 45 and 90d in Lc. Supplementation showed decreased population of enterobacteria and increased the population of lactobacilli. We did not observe significant differences between NC and LC in the total anaerobic population.Cytokines in cell culture supernatant showed a decrease in IFNγ and TNFα, increased values of IL-10, IL-12 and IL-3, and we observed tendency to normalize IL-6 values of TB in Lc. The immune cells in Lc group in Timo, at 45d showed decrease in CD4+ populations, and significantly increased at 90d respect to NC, while we observed in NC CD4+/CD8+ population a significant increase at 90d (p <0.05) respect to LC (90d CD4+/CD8+: NC = 80.72 ± 0.32, LC = 0.45 ± 0.06).Oral supplementation and in vitro stimulation with complete bacteria and/or its cell wall produced effects in the thymus at different periods of time in mice.
publishDate 2018
dc.date.none.fl_str_mv 2018
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/215766
Effect of L. casei CRL 431 and its cellular wall on the thymus at different ages in a mice model; LXIII Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXVI Reunión Anual de la Sociedad Argentina de Inmunología, Reunión Anual de la Sociedad Argentina de Fisiología; Mar del Plata; Argentina; 2018; 234-234
0025-7680
1669-9106
CONICET Digital
CONICET
url http://hdl.handle.net/11336/215766
identifier_str_mv Effect of L. casei CRL 431 and its cellular wall on the thymus at different ages in a mice model; LXIII Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXVI Reunión Anual de la Sociedad Argentina de Inmunología, Reunión Anual de la Sociedad Argentina de Fisiología; Mar del Plata; Argentina; 2018; 234-234
0025-7680
1669-9106
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.medicinabuenosaires.com/revistas/vol78-18/s3/vol78supIII.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Fundación Revista Medicina
publisher.none.fl_str_mv Fundación Revista Medicina
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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