Effect of a probiotic fermented milk on the thymus in BALB/c mice under non-severe protein-energy-malnutrition

Autores
Novotny Núñez, Ivanna; Maldonado Galdeano, María Carolina; Carmuega, Esteban; Weill, Ricardo; de Moreno, Maria Alejandra; Perdigón, Gabriela del Valle
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Protein–energy malnutrition (PEM) causes a significant impairment of the immune system, the thymus being one of the most affected organs. It has been demonstrated that the administration of probiotic fermented milk (PFM) recovered the intestinal barrier, histological alterations and mucosal and systemic immune functions in a non-severe malnutrition model using BALB/c mice. The aim of the present study was to evaluate, in the same model of malnutrition, the effect of a PFM added to a re-nutrition diet on the recovery of the thymus, analysing histological and functional alterations caused by malnutrition. Mice were undernourished and divided into three groups according to the dietary supplement received during re-nutrition: milk, PFM or its bacterial-free supernatant (BFS). They were compared with well-nourished and malnourished mice. PFM was the most effective re-nutrition supplement to improve the histology of the thymus, decreasing cellular apoptosis in this organ and recovering the percentage of CD4þ/CD82 single-positive thymocytes. Immature doublepositive thymocytes were increased in the malnourished control (MC). The production of different cytokines in the thymus was increased in mice given PFM, compared with the mice that received other dietary supplements and MC. Mice given the BFS presented an improvement in the thymus similar to those that received milk. We demonstrated the importance of the whole PFM supplementation on the histological and functional recovery of the thymus in a non-severe PEM model.
Fil: Novotny Núñez, Ivanna. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Centro de Referencia para Lactobacilos (i); Argentina
Fil: Maldonado Galdeano, María Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Centro de Referencia para Lactobacilos (i); Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología; Argentina
Fil: Carmuega, Esteban. Centro de Estudios en Nutrición Infantil; Argentina
Fil: Weill, Ricardo. No especifíca;
Fil: de Moreno, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Centro de Referencia para Lactobacilos (i); Argentina
Fil: Perdigón, Gabriela del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Centro de Referencia para Lactobacilos (i); Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología; Argentina
Materia
Fermented Milk
Probiotics
Thymus
Non-Severe-Protein Energy Malnutrition
Mouse Model
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/2201
Acceder
id CONICETDig_2c2b0c1fc2624ff471acb684996426bd
oai_identifier_str oai:ri.conicet.gov.ar:11336/2201
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Effect of a probiotic fermented milk on the thymus in BALB/c mice under non-severe protein-energy-malnutritionNovotny Núñez, IvannaMaldonado Galdeano, María CarolinaCarmuega, EstebanWeill, Ricardode Moreno, Maria AlejandraPerdigón, Gabriela del ValleFermented MilkProbioticsThymusNon-Severe-Protein Energy MalnutritionMouse Modelhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Protein–energy malnutrition (PEM) causes a significant impairment of the immune system, the thymus being one of the most affected organs. It has been demonstrated that the administration of probiotic fermented milk (PFM) recovered the intestinal barrier, histological alterations and mucosal and systemic immune functions in a non-severe malnutrition model using BALB/c mice. The aim of the present study was to evaluate, in the same model of malnutrition, the effect of a PFM added to a re-nutrition diet on the recovery of the thymus, analysing histological and functional alterations caused by malnutrition. Mice were undernourished and divided into three groups according to the dietary supplement received during re-nutrition: milk, PFM or its bacterial-free supernatant (BFS). They were compared with well-nourished and malnourished mice. PFM was the most effective re-nutrition supplement to improve the histology of the thymus, decreasing cellular apoptosis in this organ and recovering the percentage of CD4þ/CD82 single-positive thymocytes. Immature doublepositive thymocytes were increased in the malnourished control (MC). The production of different cytokines in the thymus was increased in mice given PFM, compared with the mice that received other dietary supplements and MC. Mice given the BFS presented an improvement in the thymus similar to those that received milk. We demonstrated the importance of the whole PFM supplementation on the histological and functional recovery of the thymus in a non-severe PEM model.Fil: Novotny Núñez, Ivanna. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Centro de Referencia para Lactobacilos (i); ArgentinaFil: Maldonado Galdeano, María Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Centro de Referencia para Lactobacilos (i); Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología; ArgentinaFil: Carmuega, Esteban. Centro de Estudios en Nutrición Infantil; ArgentinaFil: Weill, Ricardo. No especifíca;Fil: de Moreno, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Centro de Referencia para Lactobacilos (i); ArgentinaFil: Perdigón, Gabriela del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Centro de Referencia para Lactobacilos (i); Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología; ArgentinaCambridge University Press2013-08-28info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/octet-streamapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/2201Novotny Núñez, Ivanna; Maldonado Galdeano, María Carolina; Carmuega, Esteban; Weill, Ricardo; de Moreno, Maria Alejandra; et al.; Effect of a probiotic fermented milk on the thymus in BALB/c mice under non-severe protein-energy-malnutrition; Cambridge University Press; British Journal of Nutrition; 110; 3; 28-8-2013; 500-5081475-2662enginfo:eu-repo/semantics/altIdentifier/url/http://journals.cambridge.org/action/displayAbstract?fromPage=online&aid=8957495&fileId=S0007114512005302info:eu-repo/semantics/altIdentifier/doi/10.1017/S0007114512005302info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:52:49Zoai:ri.conicet.gov.ar:11336/2201instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:52:49.835CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effect of a probiotic fermented milk on the thymus in BALB/c mice under non-severe protein-energy-malnutrition
title Effect of a probiotic fermented milk on the thymus in BALB/c mice under non-severe protein-energy-malnutrition
spellingShingle Effect of a probiotic fermented milk on the thymus in BALB/c mice under non-severe protein-energy-malnutrition
Novotny Núñez, Ivanna
Fermented Milk
Probiotics
Thymus
Non-Severe-Protein Energy Malnutrition
Mouse Model
title_short Effect of a probiotic fermented milk on the thymus in BALB/c mice under non-severe protein-energy-malnutrition
title_full Effect of a probiotic fermented milk on the thymus in BALB/c mice under non-severe protein-energy-malnutrition
title_fullStr Effect of a probiotic fermented milk on the thymus in BALB/c mice under non-severe protein-energy-malnutrition
title_full_unstemmed Effect of a probiotic fermented milk on the thymus in BALB/c mice under non-severe protein-energy-malnutrition
title_sort Effect of a probiotic fermented milk on the thymus in BALB/c mice under non-severe protein-energy-malnutrition
dc.creator.none.fl_str_mv Novotny Núñez, Ivanna
Maldonado Galdeano, María Carolina
Carmuega, Esteban
Weill, Ricardo
de Moreno, Maria Alejandra
Perdigón, Gabriela del Valle
author Novotny Núñez, Ivanna
author_facet Novotny Núñez, Ivanna
Maldonado Galdeano, María Carolina
Carmuega, Esteban
Weill, Ricardo
de Moreno, Maria Alejandra
Perdigón, Gabriela del Valle
author_role author
author2 Maldonado Galdeano, María Carolina
Carmuega, Esteban
Weill, Ricardo
de Moreno, Maria Alejandra
Perdigón, Gabriela del Valle
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Fermented Milk
Probiotics
Thymus
Non-Severe-Protein Energy Malnutrition
Mouse Model
topic Fermented Milk
Probiotics
Thymus
Non-Severe-Protein Energy Malnutrition
Mouse Model
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Protein–energy malnutrition (PEM) causes a significant impairment of the immune system, the thymus being one of the most affected organs. It has been demonstrated that the administration of probiotic fermented milk (PFM) recovered the intestinal barrier, histological alterations and mucosal and systemic immune functions in a non-severe malnutrition model using BALB/c mice. The aim of the present study was to evaluate, in the same model of malnutrition, the effect of a PFM added to a re-nutrition diet on the recovery of the thymus, analysing histological and functional alterations caused by malnutrition. Mice were undernourished and divided into three groups according to the dietary supplement received during re-nutrition: milk, PFM or its bacterial-free supernatant (BFS). They were compared with well-nourished and malnourished mice. PFM was the most effective re-nutrition supplement to improve the histology of the thymus, decreasing cellular apoptosis in this organ and recovering the percentage of CD4þ/CD82 single-positive thymocytes. Immature doublepositive thymocytes were increased in the malnourished control (MC). The production of different cytokines in the thymus was increased in mice given PFM, compared with the mice that received other dietary supplements and MC. Mice given the BFS presented an improvement in the thymus similar to those that received milk. We demonstrated the importance of the whole PFM supplementation on the histological and functional recovery of the thymus in a non-severe PEM model.
Fil: Novotny Núñez, Ivanna. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Centro de Referencia para Lactobacilos (i); Argentina
Fil: Maldonado Galdeano, María Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Centro de Referencia para Lactobacilos (i); Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología; Argentina
Fil: Carmuega, Esteban. Centro de Estudios en Nutrición Infantil; Argentina
Fil: Weill, Ricardo. No especifíca;
Fil: de Moreno, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Centro de Referencia para Lactobacilos (i); Argentina
Fil: Perdigón, Gabriela del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Centro de Referencia para Lactobacilos (i); Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología; Argentina
description Protein–energy malnutrition (PEM) causes a significant impairment of the immune system, the thymus being one of the most affected organs. It has been demonstrated that the administration of probiotic fermented milk (PFM) recovered the intestinal barrier, histological alterations and mucosal and systemic immune functions in a non-severe malnutrition model using BALB/c mice. The aim of the present study was to evaluate, in the same model of malnutrition, the effect of a PFM added to a re-nutrition diet on the recovery of the thymus, analysing histological and functional alterations caused by malnutrition. Mice were undernourished and divided into three groups according to the dietary supplement received during re-nutrition: milk, PFM or its bacterial-free supernatant (BFS). They were compared with well-nourished and malnourished mice. PFM was the most effective re-nutrition supplement to improve the histology of the thymus, decreasing cellular apoptosis in this organ and recovering the percentage of CD4þ/CD82 single-positive thymocytes. Immature doublepositive thymocytes were increased in the malnourished control (MC). The production of different cytokines in the thymus was increased in mice given PFM, compared with the mice that received other dietary supplements and MC. Mice given the BFS presented an improvement in the thymus similar to those that received milk. We demonstrated the importance of the whole PFM supplementation on the histological and functional recovery of the thymus in a non-severe PEM model.
publishDate 2013
dc.date.none.fl_str_mv 2013-08-28
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/2201
Novotny Núñez, Ivanna; Maldonado Galdeano, María Carolina; Carmuega, Esteban; Weill, Ricardo; de Moreno, Maria Alejandra; et al.; Effect of a probiotic fermented milk on the thymus in BALB/c mice under non-severe protein-energy-malnutrition; Cambridge University Press; British Journal of Nutrition; 110; 3; 28-8-2013; 500-508
1475-2662
url http://hdl.handle.net/11336/2201
identifier_str_mv Novotny Núñez, Ivanna; Maldonado Galdeano, María Carolina; Carmuega, Esteban; Weill, Ricardo; de Moreno, Maria Alejandra; et al.; Effect of a probiotic fermented milk on the thymus in BALB/c mice under non-severe protein-energy-malnutrition; Cambridge University Press; British Journal of Nutrition; 110; 3; 28-8-2013; 500-508
1475-2662
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://journals.cambridge.org/action/displayAbstract?fromPage=online&aid=8957495&fileId=S0007114512005302
info:eu-repo/semantics/altIdentifier/doi/10.1017/S0007114512005302
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/octet-stream
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Cambridge University Press
publisher.none.fl_str_mv Cambridge University Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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