The 18S-25S ribosomal RNA unit of yerba mate (Ilex paraguariensis A. St.-Hil.)

Autores
Aguilera, Patricia Mabel; Grabiele, Mauro; Debat, Humberto Julio; Bubillo, Rosana Elizabeth; Marti, Dardo Andrea
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
We present the first report of the transcribed 18S-25S ribosomal DNA (rDNA) unit of the South American crop Ilex paraguariensis. This tree is consumed as an infusion named "mate". Next-generation sequencing data were assembled, reference-annotated and further characterized. The assembled consensus sequence of the complete transcribed rDNA unit is 6961 nt, with a coverage of ~18,646X and 99.3% of global sequence similarity. The transcribed 18S and 25S rDNA genes of yerba mate are the first in the genus to be characterized, combined with the 5.8S that is identical to the consensus sequence of Ilex. The ITS1 and the ITS2 display species-specific regions, useful to differentiate yerba mate from adulterant taxa of the manufactured product. A replication slippage process seems significant in the evolution of the internal transcribed spacer in Ilex. The 5-external transcribed spacer consists of SR1, which encloses a large inverted repeat/hairpin region, and SR2, which is a high homology region in the Asterids clade and embrace six distinctive inverted repeat/hairpin segments. The 3ETS presents a pyrimidine-rich block associated with transcription termination, mirror-like palindromes and repetitive segments, all networking at the secondary structure level. This information will be helpful in carrying out future studies in the molecular evolution characterization of this crop and related species of Ilex.
Fil: Aguilera, Patricia Mabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas | Universidad Nacional de Misiones. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas; Argentina. Universidad Nacional de Misiones. Instituto de Biotecnologia de Misiones; Argentina
Fil: Grabiele, Mauro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas | Universidad Nacional de Misiones. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas; Argentina. Universidad Nacional de Misiones. Instituto de Biotecnologia de Misiones; Argentina
Fil: Debat, Humberto Julio. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación En Ciencias Veterinarias y Agronomicas. Instituto de Fitopatología y Fisiología Vegetal; Argentina
Fil: Bubillo, Rosana Elizabeth. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Marti, Dardo Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas | Universidad Nacional de Misiones. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas; Argentina. Universidad Nacional de Misiones. Instituto de Biotecnologia de Misiones; Argentina
Materia
AQUIFOLIACEAE
ILEX
NEXT-GENERATION SEQUENCING
MOLECULAR CHARACTERIZATION
RDNA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/44395
Acceder
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network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling The 18S-25S ribosomal RNA unit of yerba mate (Ilex paraguariensis A. St.-Hil.)Aguilera, Patricia MabelGrabiele, MauroDebat, Humberto JulioBubillo, Rosana ElizabethMarti, Dardo AndreaAQUIFOLIACEAEILEXNEXT-GENERATION SEQUENCINGMOLECULAR CHARACTERIZATIONRDNAhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1We present the first report of the transcribed 18S-25S ribosomal DNA (rDNA) unit of the South American crop Ilex paraguariensis. This tree is consumed as an infusion named "mate". Next-generation sequencing data were assembled, reference-annotated and further characterized. The assembled consensus sequence of the complete transcribed rDNA unit is 6961 nt, with a coverage of ~18,646X and 99.3% of global sequence similarity. The transcribed 18S and 25S rDNA genes of yerba mate are the first in the genus to be characterized, combined with the 5.8S that is identical to the consensus sequence of Ilex. The ITS1 and the ITS2 display species-specific regions, useful to differentiate yerba mate from adulterant taxa of the manufactured product. A replication slippage process seems significant in the evolution of the internal transcribed spacer in Ilex. The 5-external transcribed spacer consists of SR1, which encloses a large inverted repeat/hairpin region, and SR2, which is a high homology region in the Asterids clade and embrace six distinctive inverted repeat/hairpin segments. The 3ETS presents a pyrimidine-rich block associated with transcription termination, mirror-like palindromes and repetitive segments, all networking at the secondary structure level. This information will be helpful in carrying out future studies in the molecular evolution characterization of this crop and related species of Ilex.Fil: Aguilera, Patricia Mabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas | Universidad Nacional de Misiones. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas; Argentina. Universidad Nacional de Misiones. Instituto de Biotecnologia de Misiones; ArgentinaFil: Grabiele, Mauro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas | Universidad Nacional de Misiones. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas; Argentina. Universidad Nacional de Misiones. Instituto de Biotecnologia de Misiones; ArgentinaFil: Debat, Humberto Julio. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación En Ciencias Veterinarias y Agronomicas. Instituto de Fitopatología y Fisiología Vegetal; ArgentinaFil: Bubillo, Rosana Elizabeth. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Marti, Dardo Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas | Universidad Nacional de Misiones. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas; Argentina. Universidad Nacional de Misiones. Instituto de Biotecnologia de Misiones; ArgentinaTaylor & Francis Ltd2016-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/44395Aguilera, Patricia Mabel; Grabiele, Mauro; Debat, Humberto Julio; Bubillo, Rosana Elizabeth; Marti, Dardo Andrea; The 18S-25S ribosomal RNA unit of yerba mate (Ilex paraguariensis A. St.-Hil.); Taylor & Francis Ltd; Plant Biosystems; 150; 6; 2-2016; 1-141126-3504CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.tandfonline.com/doi/abs/10.1080/11263504.2015.1018982info:eu-repo/semantics/altIdentifier/doi/10.1080/11263504.2015.1018982info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:20:33Zoai:ri.conicet.gov.ar:11336/44395instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:20:33.378CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv The 18S-25S ribosomal RNA unit of yerba mate (Ilex paraguariensis A. St.-Hil.)
title The 18S-25S ribosomal RNA unit of yerba mate (Ilex paraguariensis A. St.-Hil.)
spellingShingle The 18S-25S ribosomal RNA unit of yerba mate (Ilex paraguariensis A. St.-Hil.)
Aguilera, Patricia Mabel
AQUIFOLIACEAE
ILEX
NEXT-GENERATION SEQUENCING
MOLECULAR CHARACTERIZATION
RDNA
title_short The 18S-25S ribosomal RNA unit of yerba mate (Ilex paraguariensis A. St.-Hil.)
title_full The 18S-25S ribosomal RNA unit of yerba mate (Ilex paraguariensis A. St.-Hil.)
title_fullStr The 18S-25S ribosomal RNA unit of yerba mate (Ilex paraguariensis A. St.-Hil.)
title_full_unstemmed The 18S-25S ribosomal RNA unit of yerba mate (Ilex paraguariensis A. St.-Hil.)
title_sort The 18S-25S ribosomal RNA unit of yerba mate (Ilex paraguariensis A. St.-Hil.)
dc.creator.none.fl_str_mv Aguilera, Patricia Mabel
Grabiele, Mauro
Debat, Humberto Julio
Bubillo, Rosana Elizabeth
Marti, Dardo Andrea
author Aguilera, Patricia Mabel
author_facet Aguilera, Patricia Mabel
Grabiele, Mauro
Debat, Humberto Julio
Bubillo, Rosana Elizabeth
Marti, Dardo Andrea
author_role author
author2 Grabiele, Mauro
Debat, Humberto Julio
Bubillo, Rosana Elizabeth
Marti, Dardo Andrea
author2_role author
author
author
author
dc.subject.none.fl_str_mv AQUIFOLIACEAE
ILEX
NEXT-GENERATION SEQUENCING
MOLECULAR CHARACTERIZATION
RDNA
topic AQUIFOLIACEAE
ILEX
NEXT-GENERATION SEQUENCING
MOLECULAR CHARACTERIZATION
RDNA
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv We present the first report of the transcribed 18S-25S ribosomal DNA (rDNA) unit of the South American crop Ilex paraguariensis. This tree is consumed as an infusion named "mate". Next-generation sequencing data were assembled, reference-annotated and further characterized. The assembled consensus sequence of the complete transcribed rDNA unit is 6961 nt, with a coverage of ~18,646X and 99.3% of global sequence similarity. The transcribed 18S and 25S rDNA genes of yerba mate are the first in the genus to be characterized, combined with the 5.8S that is identical to the consensus sequence of Ilex. The ITS1 and the ITS2 display species-specific regions, useful to differentiate yerba mate from adulterant taxa of the manufactured product. A replication slippage process seems significant in the evolution of the internal transcribed spacer in Ilex. The 5-external transcribed spacer consists of SR1, which encloses a large inverted repeat/hairpin region, and SR2, which is a high homology region in the Asterids clade and embrace six distinctive inverted repeat/hairpin segments. The 3ETS presents a pyrimidine-rich block associated with transcription termination, mirror-like palindromes and repetitive segments, all networking at the secondary structure level. This information will be helpful in carrying out future studies in the molecular evolution characterization of this crop and related species of Ilex.
Fil: Aguilera, Patricia Mabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas | Universidad Nacional de Misiones. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas; Argentina. Universidad Nacional de Misiones. Instituto de Biotecnologia de Misiones; Argentina
Fil: Grabiele, Mauro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas | Universidad Nacional de Misiones. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas; Argentina. Universidad Nacional de Misiones. Instituto de Biotecnologia de Misiones; Argentina
Fil: Debat, Humberto Julio. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación En Ciencias Veterinarias y Agronomicas. Instituto de Fitopatología y Fisiología Vegetal; Argentina
Fil: Bubillo, Rosana Elizabeth. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Marti, Dardo Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas | Universidad Nacional de Misiones. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas; Argentina. Universidad Nacional de Misiones. Instituto de Biotecnologia de Misiones; Argentina
description We present the first report of the transcribed 18S-25S ribosomal DNA (rDNA) unit of the South American crop Ilex paraguariensis. This tree is consumed as an infusion named "mate". Next-generation sequencing data were assembled, reference-annotated and further characterized. The assembled consensus sequence of the complete transcribed rDNA unit is 6961 nt, with a coverage of ~18,646X and 99.3% of global sequence similarity. The transcribed 18S and 25S rDNA genes of yerba mate are the first in the genus to be characterized, combined with the 5.8S that is identical to the consensus sequence of Ilex. The ITS1 and the ITS2 display species-specific regions, useful to differentiate yerba mate from adulterant taxa of the manufactured product. A replication slippage process seems significant in the evolution of the internal transcribed spacer in Ilex. The 5-external transcribed spacer consists of SR1, which encloses a large inverted repeat/hairpin region, and SR2, which is a high homology region in the Asterids clade and embrace six distinctive inverted repeat/hairpin segments. The 3ETS presents a pyrimidine-rich block associated with transcription termination, mirror-like palindromes and repetitive segments, all networking at the secondary structure level. This information will be helpful in carrying out future studies in the molecular evolution characterization of this crop and related species of Ilex.
publishDate 2016
dc.date.none.fl_str_mv 2016-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/44395
Aguilera, Patricia Mabel; Grabiele, Mauro; Debat, Humberto Julio; Bubillo, Rosana Elizabeth; Marti, Dardo Andrea; The 18S-25S ribosomal RNA unit of yerba mate (Ilex paraguariensis A. St.-Hil.); Taylor & Francis Ltd; Plant Biosystems; 150; 6; 2-2016; 1-14
1126-3504
CONICET Digital
CONICET
url http://hdl.handle.net/11336/44395
identifier_str_mv Aguilera, Patricia Mabel; Grabiele, Mauro; Debat, Humberto Julio; Bubillo, Rosana Elizabeth; Marti, Dardo Andrea; The 18S-25S ribosomal RNA unit of yerba mate (Ilex paraguariensis A. St.-Hil.); Taylor & Francis Ltd; Plant Biosystems; 150; 6; 2-2016; 1-14
1126-3504
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.tandfonline.com/doi/abs/10.1080/11263504.2015.1018982
info:eu-repo/semantics/altIdentifier/doi/10.1080/11263504.2015.1018982
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Taylor & Francis Ltd
publisher.none.fl_str_mv Taylor & Francis Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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