Substrate engagement of integrins α5 β1 and αv β3 is necessary, but not sufficient, for high directional persistence in migration on fibronectin

Autores
Missirlis, Dimitris; Haraszti, Tamás; Scheele, Catharina v. C.; Wiegand, Tina; Diaz, Carolina; Neubauer, Stefanie; Rechenmacher, Florian; Kessler, Horst; Spatz, Joachim P.
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The interplay between specific integrin-mediated matrix adhesion and directional persistence in cell migration is not well understood. Here, we characterized fibroblast adhesion and migration on the extracellular matrix glycoproteins fibronectin and vitronectin, focusing on the role of α5 β1 and αv β3 integrins. Fibroblasts manifested high directional persistence in migration on fibronectin-, but not vitronectin-coated substrates, in a ligand density-dependent manner. Fibronectin stimulated α5 β1-dependent organization of the actin cytoskeleton into oriented, ventral stress fibers, and assembly of dynamic, polarized protrusions, characterized as regions free of stress fibers and rich in nascent adhesions at their edge. Such protrusions correlated with persistent, local leading edge advancement, but were not sufficient, nor necessary for directional migration over longer times. Selective blocking of αv β3 or α5 β1 integrins using small molecule integrin antagonists reduced directional persistence on fibronectin, indicating integrin cooperativity in maintaining directionality. On the other hand, patterned substrates, designed to selectively engage either integrin, or their combination, were not sufficient to establish directional migration. Overall, our study demonstrates adhesive coating-dependent regulation of directional persistence in fibroblast migration and challenges the generality of the previously suggested role of β1 and β3 integrins in directional migration.
Fil: Missirlis, Dimitris. Max Planck Institute for Intelligent Systems; Alemania. University of Heidelberg; Alemania
Fil: Haraszti, Tamás. Max Planck Institute for Intelligent Systems; Alemania. University of Heidelberg; Alemania
Fil: Scheele, Catharina v. C.. Max Planck Institute for Intelligent Systems; Alemania. University of Heidelberg; Alemania
Fil: Wiegand, Tina. University of Heidelberg; Alemania. Max Planck Institute for Intelligent Systems; Alemania
Fil: Diaz, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina. Max Planck Institute for Intelligent Systems; Alemania. University of Heidelberg; Alemania
Fil: Neubauer, Stefanie. Technische Universitat Munchen; Alemania
Fil: Rechenmacher, Florian. Technische Universitat Munchen; Alemania
Fil: Kessler, Horst. Technische Universitat Munchen; Alemania
Fil: Spatz, Joachim P.. University of Heidelberg; Alemania. Max Planck Institute for Intelligent Systems; Alemania
Materia
FOCAL ADHESION
INTEGRIN SIGNALING
FIBRONECTIN
MIGRATION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/49069
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Substrate engagement of integrins α5 β1 and αv β3 is necessary, but not sufficient, for high directional persistence in migration on fibronectinMissirlis, DimitrisHaraszti, TamásScheele, Catharina v. C.Wiegand, TinaDiaz, CarolinaNeubauer, StefanieRechenmacher, FlorianKessler, HorstSpatz, Joachim P.FOCAL ADHESIONINTEGRIN SIGNALINGFIBRONECTINMIGRATIONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The interplay between specific integrin-mediated matrix adhesion and directional persistence in cell migration is not well understood. Here, we characterized fibroblast adhesion and migration on the extracellular matrix glycoproteins fibronectin and vitronectin, focusing on the role of α5 β1 and αv β3 integrins. Fibroblasts manifested high directional persistence in migration on fibronectin-, but not vitronectin-coated substrates, in a ligand density-dependent manner. Fibronectin stimulated α5 β1-dependent organization of the actin cytoskeleton into oriented, ventral stress fibers, and assembly of dynamic, polarized protrusions, characterized as regions free of stress fibers and rich in nascent adhesions at their edge. Such protrusions correlated with persistent, local leading edge advancement, but were not sufficient, nor necessary for directional migration over longer times. Selective blocking of αv β3 or α5 β1 integrins using small molecule integrin antagonists reduced directional persistence on fibronectin, indicating integrin cooperativity in maintaining directionality. On the other hand, patterned substrates, designed to selectively engage either integrin, or their combination, were not sufficient to establish directional migration. Overall, our study demonstrates adhesive coating-dependent regulation of directional persistence in fibroblast migration and challenges the generality of the previously suggested role of β1 and β3 integrins in directional migration.Fil: Missirlis, Dimitris. Max Planck Institute for Intelligent Systems; Alemania. University of Heidelberg; AlemaniaFil: Haraszti, Tamás. Max Planck Institute for Intelligent Systems; Alemania. University of Heidelberg; AlemaniaFil: Scheele, Catharina v. C.. Max Planck Institute for Intelligent Systems; Alemania. University of Heidelberg; AlemaniaFil: Wiegand, Tina. University of Heidelberg; Alemania. Max Planck Institute for Intelligent Systems; AlemaniaFil: Diaz, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina. Max Planck Institute for Intelligent Systems; Alemania. University of Heidelberg; AlemaniaFil: Neubauer, Stefanie. Technische Universitat Munchen; AlemaniaFil: Rechenmacher, Florian. Technische Universitat Munchen; AlemaniaFil: Kessler, Horst. Technische Universitat Munchen; AlemaniaFil: Spatz, Joachim P.. University of Heidelberg; Alemania. Max Planck Institute for Intelligent Systems; AlemaniaNature Publishing Group2016-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/49069Missirlis, Dimitris; Haraszti, Tamás; Scheele, Catharina v. C.; Wiegand, Tina; Diaz, Carolina; et al.; Substrate engagement of integrins α5 β1 and αv β3 is necessary, but not sufficient, for high directional persistence in migration on fibronectin; Nature Publishing Group; Scientific Reports; 6; 3-2016; 1-18; 232582045-2322CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1038/srep23258info:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/srep23258info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:43:15Zoai:ri.conicet.gov.ar:11336/49069instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:43:15.869CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Substrate engagement of integrins α5 β1 and αv β3 is necessary, but not sufficient, for high directional persistence in migration on fibronectin
title Substrate engagement of integrins α5 β1 and αv β3 is necessary, but not sufficient, for high directional persistence in migration on fibronectin
spellingShingle Substrate engagement of integrins α5 β1 and αv β3 is necessary, but not sufficient, for high directional persistence in migration on fibronectin
Missirlis, Dimitris
FOCAL ADHESION
INTEGRIN SIGNALING
FIBRONECTIN
MIGRATION
title_short Substrate engagement of integrins α5 β1 and αv β3 is necessary, but not sufficient, for high directional persistence in migration on fibronectin
title_full Substrate engagement of integrins α5 β1 and αv β3 is necessary, but not sufficient, for high directional persistence in migration on fibronectin
title_fullStr Substrate engagement of integrins α5 β1 and αv β3 is necessary, but not sufficient, for high directional persistence in migration on fibronectin
title_full_unstemmed Substrate engagement of integrins α5 β1 and αv β3 is necessary, but not sufficient, for high directional persistence in migration on fibronectin
title_sort Substrate engagement of integrins α5 β1 and αv β3 is necessary, but not sufficient, for high directional persistence in migration on fibronectin
dc.creator.none.fl_str_mv Missirlis, Dimitris
Haraszti, Tamás
Scheele, Catharina v. C.
Wiegand, Tina
Diaz, Carolina
Neubauer, Stefanie
Rechenmacher, Florian
Kessler, Horst
Spatz, Joachim P.
author Missirlis, Dimitris
author_facet Missirlis, Dimitris
Haraszti, Tamás
Scheele, Catharina v. C.
Wiegand, Tina
Diaz, Carolina
Neubauer, Stefanie
Rechenmacher, Florian
Kessler, Horst
Spatz, Joachim P.
author_role author
author2 Haraszti, Tamás
Scheele, Catharina v. C.
Wiegand, Tina
Diaz, Carolina
Neubauer, Stefanie
Rechenmacher, Florian
Kessler, Horst
Spatz, Joachim P.
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv FOCAL ADHESION
INTEGRIN SIGNALING
FIBRONECTIN
MIGRATION
topic FOCAL ADHESION
INTEGRIN SIGNALING
FIBRONECTIN
MIGRATION
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The interplay between specific integrin-mediated matrix adhesion and directional persistence in cell migration is not well understood. Here, we characterized fibroblast adhesion and migration on the extracellular matrix glycoproteins fibronectin and vitronectin, focusing on the role of α5 β1 and αv β3 integrins. Fibroblasts manifested high directional persistence in migration on fibronectin-, but not vitronectin-coated substrates, in a ligand density-dependent manner. Fibronectin stimulated α5 β1-dependent organization of the actin cytoskeleton into oriented, ventral stress fibers, and assembly of dynamic, polarized protrusions, characterized as regions free of stress fibers and rich in nascent adhesions at their edge. Such protrusions correlated with persistent, local leading edge advancement, but were not sufficient, nor necessary for directional migration over longer times. Selective blocking of αv β3 or α5 β1 integrins using small molecule integrin antagonists reduced directional persistence on fibronectin, indicating integrin cooperativity in maintaining directionality. On the other hand, patterned substrates, designed to selectively engage either integrin, or their combination, were not sufficient to establish directional migration. Overall, our study demonstrates adhesive coating-dependent regulation of directional persistence in fibroblast migration and challenges the generality of the previously suggested role of β1 and β3 integrins in directional migration.
Fil: Missirlis, Dimitris. Max Planck Institute for Intelligent Systems; Alemania. University of Heidelberg; Alemania
Fil: Haraszti, Tamás. Max Planck Institute for Intelligent Systems; Alemania. University of Heidelberg; Alemania
Fil: Scheele, Catharina v. C.. Max Planck Institute for Intelligent Systems; Alemania. University of Heidelberg; Alemania
Fil: Wiegand, Tina. University of Heidelberg; Alemania. Max Planck Institute for Intelligent Systems; Alemania
Fil: Diaz, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina. Max Planck Institute for Intelligent Systems; Alemania. University of Heidelberg; Alemania
Fil: Neubauer, Stefanie. Technische Universitat Munchen; Alemania
Fil: Rechenmacher, Florian. Technische Universitat Munchen; Alemania
Fil: Kessler, Horst. Technische Universitat Munchen; Alemania
Fil: Spatz, Joachim P.. University of Heidelberg; Alemania. Max Planck Institute for Intelligent Systems; Alemania
description The interplay between specific integrin-mediated matrix adhesion and directional persistence in cell migration is not well understood. Here, we characterized fibroblast adhesion and migration on the extracellular matrix glycoproteins fibronectin and vitronectin, focusing on the role of α5 β1 and αv β3 integrins. Fibroblasts manifested high directional persistence in migration on fibronectin-, but not vitronectin-coated substrates, in a ligand density-dependent manner. Fibronectin stimulated α5 β1-dependent organization of the actin cytoskeleton into oriented, ventral stress fibers, and assembly of dynamic, polarized protrusions, characterized as regions free of stress fibers and rich in nascent adhesions at their edge. Such protrusions correlated with persistent, local leading edge advancement, but were not sufficient, nor necessary for directional migration over longer times. Selective blocking of αv β3 or α5 β1 integrins using small molecule integrin antagonists reduced directional persistence on fibronectin, indicating integrin cooperativity in maintaining directionality. On the other hand, patterned substrates, designed to selectively engage either integrin, or their combination, were not sufficient to establish directional migration. Overall, our study demonstrates adhesive coating-dependent regulation of directional persistence in fibroblast migration and challenges the generality of the previously suggested role of β1 and β3 integrins in directional migration.
publishDate 2016
dc.date.none.fl_str_mv 2016-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/49069
Missirlis, Dimitris; Haraszti, Tamás; Scheele, Catharina v. C.; Wiegand, Tina; Diaz, Carolina; et al.; Substrate engagement of integrins α5 β1 and αv β3 is necessary, but not sufficient, for high directional persistence in migration on fibronectin; Nature Publishing Group; Scientific Reports; 6; 3-2016; 1-18; 23258
2045-2322
CONICET Digital
CONICET
url http://hdl.handle.net/11336/49069
identifier_str_mv Missirlis, Dimitris; Haraszti, Tamás; Scheele, Catharina v. C.; Wiegand, Tina; Diaz, Carolina; et al.; Substrate engagement of integrins α5 β1 and αv β3 is necessary, but not sufficient, for high directional persistence in migration on fibronectin; Nature Publishing Group; Scientific Reports; 6; 3-2016; 1-18; 23258
2045-2322
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1038/srep23258
info:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/srep23258
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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