Performance assessment of qPCR assays targeting human- and ruminant-associated Bacteroidetes for microbial source tracking across sixteen countries on six continents

Autores: Reischer, Georg H.; Ebdon, James E.; Bauer, Johanna M.; Schuster, Nathalie; Ahmed, Warish; Åström, Johan; Blanch, Anicet R.; Blöschl, Günter; Byamukama, Denis; Coakley, Tricia; Ferguson, Christobel; Goshu, Goraw; Ko, GwangPyo; de Roda Husman, Ana Maria; Mushi, Douglas; Poma, Hugo Ramiro; Pradhan, Bandana; Rajal, Verónica Beatriz; Schade, Margit A.; Sommer, Regina; Taylor, Huw; Toth, Erika M.; Vrajmasu, Virgil; Wuertz, Stefan; Mach, Robert L.; Farnleitner, Andreas H.
Año de publicación: 2013
Idioma: inglés
Tipo de recurso: artículo
Estado: versión publicada
Descripción: Numerous quantitative PCR assays for microbial fecal source tracking (MST) have been developed and evaluated in recent years. Widespread application has been hindered by a lack of knowledge regarding the geographical stability and hence applicability of such methods beyond the regional level. This study assessed the performance of five previously reported quantitative PCR assays targeting human-, cattle- or ruminant-associated Bacteroidetes populations on 280 human and animal fecal samples from 16 countries across six continents. The tested cattle-associated markers were shown to be ruminant-associated. The quantitative distributions of marker concentrations in target and non target samples proved to be essential for the assessment of assay performance and were used to establish a new metric for quantitative source-specificity. In general, this study demonstrates that stable target populations required for marker-based MST occur around the globe. Ruminant-associated marker concentrations were strongly correlated with total intestinal Bacteroidetes populations and with each other, indicating that the detected ruminant-associated populations seem to be part of the intestinal core microbiome of ruminants worldwide. Consequently tested ruminant targeted assays appear to be suitable quantitative MST tools beyond the regional level while the targeted human-associated populations seem to be less prevalent and stable suggesting potential for improvements in human-targeted methods.
Fil: Reischer, Georg H.. Vienna University of Technology; Austria. InterUniversity Cooperation Centre Water & Health; Austria
Fil: Ebdon, James E.. University of Brighton. School of Environment and Technology. Environment & Public Health Research Unit ; Reino Unido
Fil: Bauer, Johanna M.. Vienna University of Technology; Austria
Fil: Schuster, Nathalie. Vienna University of Technology; Austria
Fil: Ahmed, Warish. CSIRO Land and Water; Australia
Fil: Åström, Johan. Chalmers University Of Technology; Suecia
Fil: Blanch, Anicet R.. Universidad de Barcelona. Departamento de Bioquimica; España
Fil: Blöschl, Günter. Vienna University of Technology; Austria
Fil: Byamukama, Denis. Makerere University. Department of Biochemistry; Uganda
Fil: Coakley, Tricia. University Of Kentucky; Estados Unidos
Fil: Ferguson, Christobel. ALS Water Sciences Group; Australia
Fil: Goshu, Goraw. Bahir Dar University. Blue Nile Water Institute. College of Agriculture and Environmental Sciences; Etiopía
Fil: Ko, GwangPyo. Seoul National University; Corea del Sur
Fil: de Roda Husman, Ana Maria. National Institute for Public Health and the Environment ; Países Bajos
Fil: Mushi, Douglas. Sokoine University; Tanzania
Fil: Poma, Hugo Ramiro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Salta. Instituto de Investigación para la Industria Química (i); Argentina
Fil: Pradhan, Bandana. Tribhuvan University; Nepal
Fil: Rajal, Verónica Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Salta. Instituto de Investigación para la Industria Química (i); Argentina
Fil: Schade, Margit A.. Bavarian Environment Agency; Alemania
Fil: Sommer, Regina. InterUniversity Cooperation Centre Water & Health; Austria. Medical University of Vienna. Institute for Hygiene and Applied Immunology, Water Hygiene; Austria
Fil: Taylor, Huw. University of Brighton. School of Environment and Technology. Environment & Public Health Research Unit ; Reino Unido
Fil: Toth, Erika M.. Eötvös Lorand University. Department of Microbiology. Biological Institute; Hungría
Fil: Vrajmasu, Virgil. Veterinary State Laboratory; Rumania
Fil: Wuertz, Stefan. University Of California At Davis; Estados Unidos
Fil: Mach, Robert L.. Vienna University of Technology; Austria
Fil: Farnleitner, Andreas H.. Vienna University of Technology; Austria. InterUniversity Cooperation Centre Water & Health; Austria
Materia: Microbial Source Tracking
Pollution Microbiology
Fecal Pollution
Bacteroidetes
Quantitative Real-Time Pcr
Genetic Markers
Water Quality
Nivel de accesibilidad: acceso abierto
Condiciones de uso: https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
Institución: Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador: oai:ri.conicet.gov.ar:11336/4720

Acceder

id	CONICETDig_0d4ad74feb4017c88752219e555f27c1
oai_identifier_str	oai:ri.conicet.gov.ar:11336/4720
network_acronym_str	CONICETDig
repository_id_str	3498
network_name_str	CONICET Digital (CONICET)
spelling	Performance assessment of qPCR assays targeting human- and ruminant-associated Bacteroidetes for microbial source tracking across sixteen countries on six continentsReischer, Georg H.Ebdon, James E.Bauer, Johanna M.Schuster, NathalieAhmed, WarishÅström, JohanBlanch, Anicet R.Blöschl, GünterByamukama, DenisCoakley, TriciaFerguson, ChristobelGoshu, GorawKo, GwangPyode Roda Husman, Ana MariaMushi, DouglasPoma, Hugo RamiroPradhan, BandanaRajal, Verónica BeatrizSchade, Margit A.Sommer, ReginaTaylor, HuwToth, Erika M.Vrajmasu, VirgilWuertz, StefanMach, Robert L.Farnleitner, Andreas H.Microbial Source TrackingPollution MicrobiologyFecal PollutionBacteroidetesQuantitative Real-Time PcrGenetic MarkersWater Qualityhttps://purl.org/becyt/ford/2.8https://purl.org/becyt/ford/2Numerous quantitative PCR assays for microbial fecal source tracking (MST) have been developed and evaluated in recent years. Widespread application has been hindered by a lack of knowledge regarding the geographical stability and hence applicability of such methods beyond the regional level. This study assessed the performance of five previously reported quantitative PCR assays targeting human-, cattle- or ruminant-associated Bacteroidetes populations on 280 human and animal fecal samples from 16 countries across six continents. The tested cattle-associated markers were shown to be ruminant-associated. The quantitative distributions of marker concentrations in target and non target samples proved to be essential for the assessment of assay performance and were used to establish a new metric for quantitative source-specificity. In general, this study demonstrates that stable target populations required for marker-based MST occur around the globe. Ruminant-associated marker concentrations were strongly correlated with total intestinal Bacteroidetes populations and with each other, indicating that the detected ruminant-associated populations seem to be part of the intestinal core microbiome of ruminants worldwide. Consequently tested ruminant targeted assays appear to be suitable quantitative MST tools beyond the regional level while the targeted human-associated populations seem to be less prevalent and stable suggesting potential for improvements in human-targeted methods.Fil: Reischer, Georg H.. Vienna University of Technology; Austria. InterUniversity Cooperation Centre Water & Health; AustriaFil: Ebdon, James E.. University of Brighton. School of Environment and Technology. Environment & Public Health Research Unit ; Reino UnidoFil: Bauer, Johanna M.. Vienna University of Technology; AustriaFil: Schuster, Nathalie. Vienna University of Technology; AustriaFil: Ahmed, Warish. CSIRO Land and Water; AustraliaFil: Åström, Johan. Chalmers University Of Technology; SueciaFil: Blanch, Anicet R.. Universidad de Barcelona. Departamento de Bioquimica; EspañaFil: Blöschl, Günter. Vienna University of Technology; AustriaFil: Byamukama, Denis. Makerere University. Department of Biochemistry; UgandaFil: Coakley, Tricia. University Of Kentucky; Estados UnidosFil: Ferguson, Christobel. ALS Water Sciences Group; AustraliaFil: Goshu, Goraw. Bahir Dar University. Blue Nile Water Institute. College of Agriculture and Environmental Sciences; EtiopíaFil: Ko, GwangPyo. Seoul National University; Corea del SurFil: de Roda Husman, Ana Maria. National Institute for Public Health and the Environment ; Países BajosFil: Mushi, Douglas. Sokoine University; TanzaniaFil: Poma, Hugo Ramiro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Salta. Instituto de Investigación para la Industria Química (i); ArgentinaFil: Pradhan, Bandana. Tribhuvan University; NepalFil: Rajal, Verónica Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Salta. Instituto de Investigación para la Industria Química (i); ArgentinaFil: Schade, Margit A.. Bavarian Environment Agency; AlemaniaFil: Sommer, Regina. InterUniversity Cooperation Centre Water & Health; Austria. Medical University of Vienna. Institute for Hygiene and Applied Immunology, Water Hygiene; AustriaFil: Taylor, Huw. University of Brighton. School of Environment and Technology. Environment & Public Health Research Unit ; Reino UnidoFil: Toth, Erika M.. Eötvös Lorand University. Department of Microbiology. Biological Institute; HungríaFil: Vrajmasu, Virgil. Veterinary State Laboratory; RumaniaFil: Wuertz, Stefan. University Of California At Davis; Estados UnidosFil: Mach, Robert L.. Vienna University of Technology; AustriaFil: Farnleitner, Andreas H.. Vienna University of Technology; Austria. InterUniversity Cooperation Centre Water & Health; AustriaAmerican Chemical Society2013-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/4720Reischer, Georg H.; Ebdon, James E.; Bauer, Johanna M.; Schuster, Nathalie; Ahmed, Warish; et al.; Performance assessment of qPCR assays targeting human- and ruminant-associated Bacteroidetes for microbial source tracking across sixteen countries on six continents; American Chemical Society; Environmental Science & Technology; 47; 15; 6-2013; 8548-85560013-936Xenginfo:eu-repo/semantics/altIdentifier/url/http://pubs.acs.org/doi/abs/10.1021/es304367tinfo:eu-repo/semantics/altIdentifier/doi/10.1021%2Fes304367tinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:34:00Zoai:ri.conicet.gov.ar:11336/4720instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:34:00.599CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv	Performance assessment of qPCR assays targeting human- and ruminant-associated Bacteroidetes for microbial source tracking across sixteen countries on six continents
title	Performance assessment of qPCR assays targeting human- and ruminant-associated Bacteroidetes for microbial source tracking across sixteen countries on six continents
spellingShingle	Performance assessment of qPCR assays targeting human- and ruminant-associated Bacteroidetes for microbial source tracking across sixteen countries on six continents Reischer, Georg H. Microbial Source Tracking Pollution Microbiology Fecal Pollution Bacteroidetes Quantitative Real-Time Pcr Genetic Markers Water Quality
title_short	Performance assessment of qPCR assays targeting human- and ruminant-associated Bacteroidetes for microbial source tracking across sixteen countries on six continents
title_full	Performance assessment of qPCR assays targeting human- and ruminant-associated Bacteroidetes for microbial source tracking across sixteen countries on six continents
title_fullStr	Performance assessment of qPCR assays targeting human- and ruminant-associated Bacteroidetes for microbial source tracking across sixteen countries on six continents
title_full_unstemmed	Performance assessment of qPCR assays targeting human- and ruminant-associated Bacteroidetes for microbial source tracking across sixteen countries on six continents
title_sort	Performance assessment of qPCR assays targeting human- and ruminant-associated Bacteroidetes for microbial source tracking across sixteen countries on six continents
dc.creator.none.fl_str_mv	Reischer, Georg H. Ebdon, James E. Bauer, Johanna M. Schuster, Nathalie Ahmed, Warish Åström, Johan Blanch, Anicet R. Blöschl, Günter Byamukama, Denis Coakley, Tricia Ferguson, Christobel Goshu, Goraw Ko, GwangPyo de Roda Husman, Ana Maria Mushi, Douglas Poma, Hugo Ramiro Pradhan, Bandana Rajal, Verónica Beatriz Schade, Margit A. Sommer, Regina Taylor, Huw Toth, Erika M. Vrajmasu, Virgil Wuertz, Stefan Mach, Robert L. Farnleitner, Andreas H.
author	Reischer, Georg H.
author_facet	Reischer, Georg H. Ebdon, James E. Bauer, Johanna M. Schuster, Nathalie Ahmed, Warish Åström, Johan Blanch, Anicet R. Blöschl, Günter Byamukama, Denis Coakley, Tricia Ferguson, Christobel Goshu, Goraw Ko, GwangPyo de Roda Husman, Ana Maria Mushi, Douglas Poma, Hugo Ramiro Pradhan, Bandana Rajal, Verónica Beatriz Schade, Margit A. Sommer, Regina Taylor, Huw Toth, Erika M. Vrajmasu, Virgil Wuertz, Stefan Mach, Robert L. Farnleitner, Andreas H.
author_role	author
author2	Ebdon, James E. Bauer, Johanna M. Schuster, Nathalie Ahmed, Warish Åström, Johan Blanch, Anicet R. Blöschl, Günter Byamukama, Denis Coakley, Tricia Ferguson, Christobel Goshu, Goraw Ko, GwangPyo de Roda Husman, Ana Maria Mushi, Douglas Poma, Hugo Ramiro Pradhan, Bandana Rajal, Verónica Beatriz Schade, Margit A. Sommer, Regina Taylor, Huw Toth, Erika M. Vrajmasu, Virgil Wuertz, Stefan Mach, Robert L. Farnleitner, Andreas H.
author2_role	author author author author author author author author author author author author author author author author author author author author author author author author author
dc.subject.none.fl_str_mv	Microbial Source Tracking Pollution Microbiology Fecal Pollution Bacteroidetes Quantitative Real-Time Pcr Genetic Markers Water Quality
topic	Microbial Source Tracking Pollution Microbiology Fecal Pollution Bacteroidetes Quantitative Real-Time Pcr Genetic Markers Water Quality
purl_subject.fl_str_mv	https://purl.org/becyt/ford/2.8 https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv	Numerous quantitative PCR assays for microbial fecal source tracking (MST) have been developed and evaluated in recent years. Widespread application has been hindered by a lack of knowledge regarding the geographical stability and hence applicability of such methods beyond the regional level. This study assessed the performance of five previously reported quantitative PCR assays targeting human-, cattle- or ruminant-associated Bacteroidetes populations on 280 human and animal fecal samples from 16 countries across six continents. The tested cattle-associated markers were shown to be ruminant-associated. The quantitative distributions of marker concentrations in target and non target samples proved to be essential for the assessment of assay performance and were used to establish a new metric for quantitative source-specificity. In general, this study demonstrates that stable target populations required for marker-based MST occur around the globe. Ruminant-associated marker concentrations were strongly correlated with total intestinal Bacteroidetes populations and with each other, indicating that the detected ruminant-associated populations seem to be part of the intestinal core microbiome of ruminants worldwide. Consequently tested ruminant targeted assays appear to be suitable quantitative MST tools beyond the regional level while the targeted human-associated populations seem to be less prevalent and stable suggesting potential for improvements in human-targeted methods. Fil: Reischer, Georg H.. Vienna University of Technology; Austria. InterUniversity Cooperation Centre Water & Health; Austria Fil: Ebdon, James E.. University of Brighton. School of Environment and Technology. Environment & Public Health Research Unit ; Reino Unido Fil: Bauer, Johanna M.. Vienna University of Technology; Austria Fil: Schuster, Nathalie. Vienna University of Technology; Austria Fil: Ahmed, Warish. CSIRO Land and Water; Australia Fil: Åström, Johan. Chalmers University Of Technology; Suecia Fil: Blanch, Anicet R.. Universidad de Barcelona. Departamento de Bioquimica; España Fil: Blöschl, Günter. Vienna University of Technology; Austria Fil: Byamukama, Denis. Makerere University. Department of Biochemistry; Uganda Fil: Coakley, Tricia. University Of Kentucky; Estados Unidos Fil: Ferguson, Christobel. ALS Water Sciences Group; Australia Fil: Goshu, Goraw. Bahir Dar University. Blue Nile Water Institute. College of Agriculture and Environmental Sciences; Etiopía Fil: Ko, GwangPyo. Seoul National University; Corea del Sur Fil: de Roda Husman, Ana Maria. National Institute for Public Health and the Environment ; Países Bajos Fil: Mushi, Douglas. Sokoine University; Tanzania Fil: Poma, Hugo Ramiro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Salta. Instituto de Investigación para la Industria Química (i); Argentina Fil: Pradhan, Bandana. Tribhuvan University; Nepal Fil: Rajal, Verónica Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Salta. Instituto de Investigación para la Industria Química (i); Argentina Fil: Schade, Margit A.. Bavarian Environment Agency; Alemania Fil: Sommer, Regina. InterUniversity Cooperation Centre Water & Health; Austria. Medical University of Vienna. Institute for Hygiene and Applied Immunology, Water Hygiene; Austria Fil: Taylor, Huw. University of Brighton. School of Environment and Technology. Environment & Public Health Research Unit ; Reino Unido Fil: Toth, Erika M.. Eötvös Lorand University. Department of Microbiology. Biological Institute; Hungría Fil: Vrajmasu, Virgil. Veterinary State Laboratory; Rumania Fil: Wuertz, Stefan. University Of California At Davis; Estados Unidos Fil: Mach, Robert L.. Vienna University of Technology; Austria Fil: Farnleitner, Andreas H.. Vienna University of Technology; Austria. InterUniversity Cooperation Centre Water & Health; Austria
description	Numerous quantitative PCR assays for microbial fecal source tracking (MST) have been developed and evaluated in recent years. Widespread application has been hindered by a lack of knowledge regarding the geographical stability and hence applicability of such methods beyond the regional level. This study assessed the performance of five previously reported quantitative PCR assays targeting human-, cattle- or ruminant-associated Bacteroidetes populations on 280 human and animal fecal samples from 16 countries across six continents. The tested cattle-associated markers were shown to be ruminant-associated. The quantitative distributions of marker concentrations in target and non target samples proved to be essential for the assessment of assay performance and were used to establish a new metric for quantitative source-specificity. In general, this study demonstrates that stable target populations required for marker-based MST occur around the globe. Ruminant-associated marker concentrations were strongly correlated with total intestinal Bacteroidetes populations and with each other, indicating that the detected ruminant-associated populations seem to be part of the intestinal core microbiome of ruminants worldwide. Consequently tested ruminant targeted assays appear to be suitable quantitative MST tools beyond the regional level while the targeted human-associated populations seem to be less prevalent and stable suggesting potential for improvements in human-targeted methods.
publishDate	2013
dc.date.none.fl_str_mv	2013-06
dc.type.none.fl_str_mv	info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo
format	article
status_str	publishedVersion
dc.identifier.none.fl_str_mv	http://hdl.handle.net/11336/4720 Reischer, Georg H.; Ebdon, James E.; Bauer, Johanna M.; Schuster, Nathalie; Ahmed, Warish; et al.; Performance assessment of qPCR assays targeting human- and ruminant-associated Bacteroidetes for microbial source tracking across sixteen countries on six continents; American Chemical Society; Environmental Science & Technology; 47; 15; 6-2013; 8548-8556 0013-936X
url	http://hdl.handle.net/11336/4720
identifier_str_mv	Reischer, Georg H.; Ebdon, James E.; Bauer, Johanna M.; Schuster, Nathalie; Ahmed, Warish; et al.; Performance assessment of qPCR assays targeting human- and ruminant-associated Bacteroidetes for microbial source tracking across sixteen countries on six continents; American Chemical Society; Environmental Science & Technology; 47; 15; 6-2013; 8548-8556 0013-936X
dc.language.none.fl_str_mv	eng
language	eng
dc.relation.none.fl_str_mv	info:eu-repo/semantics/altIdentifier/url/http://pubs.acs.org/doi/abs/10.1021/es304367t info:eu-repo/semantics/altIdentifier/doi/10.1021%2Fes304367t
dc.rights.none.fl_str_mv	info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv	openAccess
rights_invalid_str_mv	https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv	application/pdf application/pdf
dc.publisher.none.fl_str_mv	American Chemical Society
publisher.none.fl_str_mv	American Chemical Society
dc.source.none.fl_str_mv	reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str	CONICET Digital (CONICET)
collection	CONICET Digital (CONICET)
instname_str	Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv	CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv	dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_	1844613049559810048
score	13.070432

Performance assessment of qPCR assays targeting human- and ruminant-associated Bacteroidetes for microbial source tracking across sixteen countries on six continents

Publicaciones similares