Global Distribution of Human-Associated Fecal Genetic Markers in Reference Samples from Six Continents

• Autores: Mayer, René E.; Reischer, Georg; Ixenmaier, Simone K.; Derx, Julia; Blaschke, Alfred Paul; Ebdon, James E.; Linke, Rita; Egle, Lukas; Ahmed, Warish; Blanch, Anicet R.; Byamukama, Denis; Savill, Marion; Mushi, Douglas; Cristobal, Hector Antonio; Edge, Thomas A.; Schade, Margit A.; Aslan, Asli; Brooks, Yolanda M.; Sommer, Regina; Masago, Yoshifumi; Sato, Maria I.; Taylor, Huw D.; Rose, Joan B.; Wuertz, Stefan; Shanks, Orin; Piringer, Harald; Mach, Robert L.; Savio, Domenico; Zessner, Matthias; Farnleitner, Andreas
• Año de publicación: 2018
• Idioma: inglés
• Tipo de recurso: artículo
• Materia: MICROBIAL SOURCE TRACKING; POLLUTION MICROBIOLOGY; FECAL POLLUTION; BACTEROIDETES; QUANTITATIVE PCR; GENETIC MARKERS; WATER QUALITY
• Institución: Consejo Nacional de Investigaciones Científicas y Técnicas

Numerous bacterial genetic markers are available for the molecular detection of human sources of fecal pollution in environmental waters. However, widespread application is hindered by a lack of knowledge regarding geographical stability, limiting implementation to a small number of well-characterized regions. This study investigates the geographic distribution of five human-associated genetic markers (HF183/BFDrev, HF183/BacR287, BacHum-UCD, BacH, and Lachno2) in municipal wastewaters (raw and treated) from 29 urban and rural wastewater treatment plants (750-4»400»000 population equivalents) from 13 countries spanning six continents. In addition, genetic markers were tested against 280 human and nonhuman fecal samples from domesticated, agricultural and wild animal sources. Findings revealed that all genetic markers are present in consistently high concentrations in raw (median log10 7.2-8.0 marker equivalents (ME) 100 mL-1) and biologically treated wastewater samples (median log10 4.6-6.0 ME 100 mL-1) regardless of location and population. The false positive rates of the various markers in nonhuman fecal samples ranged from 5% to 47%. Results suggest that several genetic markers have considerable potential for measuring human-associated contamination in polluted environmental waters. This will be helpful in water quality monitoring, pollution modeling and health risk assessment (as demonstrated by QMRAcatch) to guide target-oriented water safety management across the globe.
Fil: Mayer, René E.. Vienna University of Technology; Austria. Interuniversity Cooperation Centre for Water and Health; Austria
Fil: Reischer, Georg. Vienna University of Technology; Austria
Fil: Ixenmaier, Simone K.. Vienna University of Technology; Austria. Interuniversity Cooperation Centre for Water and Health; Austria
Fil: Derx, Julia. Vienna University of Technology; Austria
Fil: Blaschke, Alfred Paul. Vienna University of Technology; Austria
Fil: Ebdon, James E.. University of Brighton; Reino Unido
Fil: Linke, Rita. Vienna University of Technology; Austria. Interuniversity Cooperation Centre Water And Health; Austria
Fil: Egle, Lukas. Vienna University of Technology; Austria
Fil: Ahmed, Warish. Csiro Land And Water; Australia
Fil: Blanch, Anicet R.. Universidad de Barcelona; España
Fil: Byamukama, Denis. Makerere University; Uganda
Fil: Savill, Marion. Affordable Water Limited;
Fil: Mushi, Douglas. Sokoine University Of Agriculture; Tanzania
Fil: Cristobal, Hector Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Investigaciones para la Industria Química. Universidad Nacional de Salta. Facultad de Ingeniería. Instituto de Investigaciones para la Industria Química; Argentina
Fil: Edge, Thomas A.. Canada Centre for Inland Waters. Environment and Climate Change Canada; Canadá
Fil: Schade, Margit A.. Bavarian Environment Agency; Alemania
Fil: Aslan, Asli. Georgia Southern University; Estados Unidos
Fil: Brooks, Yolanda M.. Michigan State University; Estados Unidos
Fil: Sommer, Regina. Interuniversity Cooperation Centre Water And Health; Austria. Medizinische Universitat Wien; Austria
Fil: Masago, Yoshifumi. Tohoku University; Japón
Fil: Sato, Maria I.. Cia. Ambiental do Estado de Sao Paulo. Departamento de Análises Ambientais; Brasil
Fil: Taylor, Huw D.. University of Brighton; Reino Unido
Fil: Rose, Joan B.. Michigan State University; Estados Unidos
Fil: Wuertz, Stefan. Nanyang Technological University. Singapore Centre for Environmental Life Sciences Engineering and School of Civil and Environmental Engineering; Singapur
Fil: Shanks, Orin. U.S. Environmental Protection Agency; Estados Unidos
Fil: Piringer, Harald. Vrvis Research Center; Austria
Fil: Mach, Robert L.. Vienna University of Technology; Austria
Fil: Savio, Domenico. Karl Landsteiner University of Health Sciences; Austria
Fil: Zessner, Matthias. Vienna University of Technology; Austria
Fil: Farnleitner, Andreas. Vienna University of Technology; Austria. Interuniversity Cooperation Centre Water And Health; Austria. Karl Landsteiner University of Health Sciences; Austria