N-β-alanyldopamine metabolism, locomotor activity and sleep in Drosophila melanogaster ebony and tan mutants

Autores
Rossi, Fabiana Alejandra; Bochicchio, Pablo Alejandro; Quesada Allue, Luis Alberto; Pérez, Martín Mariano
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Drosophila melanogaster Meigen mutants for N-β-alanyldopamine (NBAD) metabolism have altered levels of NBAD, dopamine and other neurotransmitters. The ebony1 mutant strain has very low levels of NBAD and higher levels of dopamine, whereas the opposite situation is observed in the tan1 mutant. Dopamine is implicated in the control of movement, memory and arousal, as well as in the regulation of sleep and wakefulness in D. melanogaster. N-β-alanyldopamine, which is best known as a cuticle cross-linking agent, is also present in nervous tissue and has been proposed to promote locomotor activity in this fly. The daily locomotor activity and the sleep patterns of ebony1 and tan1 mutants are analyzed, and are compared with wild-type flies. The tan1 mutant shows reduced locomotor activity, whereas ebony1 shows higher levels of activity than wild-type flies, suggesting that NBAD does not promote locomotor activity. Both mutants spend less time asleep than wild-type flies during night-time; ebony shows more consolidated activity during night-time and increased sleep latency, whereas tan is unable to consolidate locomotor activity and sleep in either phase of the day. The daily level of NBAD-synthase activity is measured in vitro using wild-type and tan1 protein extracts, and the lowest NBAD synthesis is observed at the time of higher locomotor activity. The abnormalities in several parameters of the waking/sleep cycle indicate some dysfunction in the processes that regulates these behaviours in both mutants.
Fil: Rossi, Fabiana Alejandra. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Bochicchio, Pablo Alejandro. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Quesada Allue, Luis Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina
Fil: Pérez, Martín Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina
Materia
DOPAMINE
EBONY
LOCOMOTOR ACTIVITY
N-Β-ALANYLDOPAMINE
SLEEP
TAN
WILD-TYPE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/14230
Acceder
id CONICETDig_0cead0a3d14a4fcbe1c9a7b22f01b392
oai_identifier_str oai:ri.conicet.gov.ar:11336/14230
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling N-β-alanyldopamine metabolism, locomotor activity and sleep in Drosophila melanogaster ebony and tan mutantsRossi, Fabiana AlejandraBochicchio, Pablo AlejandroQuesada Allue, Luis AlbertoPérez, Martín MarianoDOPAMINEEBONYLOCOMOTOR ACTIVITYN-Β-ALANYLDOPAMINESLEEPTANWILD-TYPEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Drosophila melanogaster Meigen mutants for N-β-alanyldopamine (NBAD) metabolism have altered levels of NBAD, dopamine and other neurotransmitters. The ebony1 mutant strain has very low levels of NBAD and higher levels of dopamine, whereas the opposite situation is observed in the tan1 mutant. Dopamine is implicated in the control of movement, memory and arousal, as well as in the regulation of sleep and wakefulness in D. melanogaster. N-β-alanyldopamine, which is best known as a cuticle cross-linking agent, is also present in nervous tissue and has been proposed to promote locomotor activity in this fly. The daily locomotor activity and the sleep patterns of ebony1 and tan1 mutants are analyzed, and are compared with wild-type flies. The tan1 mutant shows reduced locomotor activity, whereas ebony1 shows higher levels of activity than wild-type flies, suggesting that NBAD does not promote locomotor activity. Both mutants spend less time asleep than wild-type flies during night-time; ebony shows more consolidated activity during night-time and increased sleep latency, whereas tan is unable to consolidate locomotor activity and sleep in either phase of the day. The daily level of NBAD-synthase activity is measured in vitro using wild-type and tan1 protein extracts, and the lowest NBAD synthesis is observed at the time of higher locomotor activity. The abnormalities in several parameters of the waking/sleep cycle indicate some dysfunction in the processes that regulates these behaviours in both mutants.Fil: Rossi, Fabiana Alejandra. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaFil: Bochicchio, Pablo Alejandro. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaFil: Quesada Allue, Luis Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; ArgentinaFil: Pérez, Martín Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; ArgentinaJohn Wiley & Sons Inc2015-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/14230Rossi, Fabiana Alejandra; Bochicchio, Pablo Alejandro; Quesada Allue, Luis Alberto; Pérez, Martín Mariano; N-β-alanyldopamine metabolism, locomotor activity and sleep in Drosophila melanogaster ebony and tan mutants; John Wiley & Sons Inc; Physiological Entomology (print); 40; 2; 6-2015; 166-1740307-6962enginfo:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/phen.12100/fullinfo:eu-repo/semantics/altIdentifier/doi/10.1111/phen.12100info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:54:09Zoai:ri.conicet.gov.ar:11336/14230instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:54:10.043CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv N-β-alanyldopamine metabolism, locomotor activity and sleep in Drosophila melanogaster ebony and tan mutants
title N-β-alanyldopamine metabolism, locomotor activity and sleep in Drosophila melanogaster ebony and tan mutants
spellingShingle N-β-alanyldopamine metabolism, locomotor activity and sleep in Drosophila melanogaster ebony and tan mutants
Rossi, Fabiana Alejandra
DOPAMINE
EBONY
LOCOMOTOR ACTIVITY
N-Β-ALANYLDOPAMINE
SLEEP
TAN
WILD-TYPE
title_short N-β-alanyldopamine metabolism, locomotor activity and sleep in Drosophila melanogaster ebony and tan mutants
title_full N-β-alanyldopamine metabolism, locomotor activity and sleep in Drosophila melanogaster ebony and tan mutants
title_fullStr N-β-alanyldopamine metabolism, locomotor activity and sleep in Drosophila melanogaster ebony and tan mutants
title_full_unstemmed N-β-alanyldopamine metabolism, locomotor activity and sleep in Drosophila melanogaster ebony and tan mutants
title_sort N-β-alanyldopamine metabolism, locomotor activity and sleep in Drosophila melanogaster ebony and tan mutants
dc.creator.none.fl_str_mv Rossi, Fabiana Alejandra
Bochicchio, Pablo Alejandro
Quesada Allue, Luis Alberto
Pérez, Martín Mariano
author Rossi, Fabiana Alejandra
author_facet Rossi, Fabiana Alejandra
Bochicchio, Pablo Alejandro
Quesada Allue, Luis Alberto
Pérez, Martín Mariano
author_role author
author2 Bochicchio, Pablo Alejandro
Quesada Allue, Luis Alberto
Pérez, Martín Mariano
author2_role author
author
author
dc.subject.none.fl_str_mv DOPAMINE
EBONY
LOCOMOTOR ACTIVITY
N-Β-ALANYLDOPAMINE
SLEEP
TAN
WILD-TYPE
topic DOPAMINE
EBONY
LOCOMOTOR ACTIVITY
N-Β-ALANYLDOPAMINE
SLEEP
TAN
WILD-TYPE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Drosophila melanogaster Meigen mutants for N-β-alanyldopamine (NBAD) metabolism have altered levels of NBAD, dopamine and other neurotransmitters. The ebony1 mutant strain has very low levels of NBAD and higher levels of dopamine, whereas the opposite situation is observed in the tan1 mutant. Dopamine is implicated in the control of movement, memory and arousal, as well as in the regulation of sleep and wakefulness in D. melanogaster. N-β-alanyldopamine, which is best known as a cuticle cross-linking agent, is also present in nervous tissue and has been proposed to promote locomotor activity in this fly. The daily locomotor activity and the sleep patterns of ebony1 and tan1 mutants are analyzed, and are compared with wild-type flies. The tan1 mutant shows reduced locomotor activity, whereas ebony1 shows higher levels of activity than wild-type flies, suggesting that NBAD does not promote locomotor activity. Both mutants spend less time asleep than wild-type flies during night-time; ebony shows more consolidated activity during night-time and increased sleep latency, whereas tan is unable to consolidate locomotor activity and sleep in either phase of the day. The daily level of NBAD-synthase activity is measured in vitro using wild-type and tan1 protein extracts, and the lowest NBAD synthesis is observed at the time of higher locomotor activity. The abnormalities in several parameters of the waking/sleep cycle indicate some dysfunction in the processes that regulates these behaviours in both mutants.
Fil: Rossi, Fabiana Alejandra. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Bochicchio, Pablo Alejandro. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Quesada Allue, Luis Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina
Fil: Pérez, Martín Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina
description Drosophila melanogaster Meigen mutants for N-β-alanyldopamine (NBAD) metabolism have altered levels of NBAD, dopamine and other neurotransmitters. The ebony1 mutant strain has very low levels of NBAD and higher levels of dopamine, whereas the opposite situation is observed in the tan1 mutant. Dopamine is implicated in the control of movement, memory and arousal, as well as in the regulation of sleep and wakefulness in D. melanogaster. N-β-alanyldopamine, which is best known as a cuticle cross-linking agent, is also present in nervous tissue and has been proposed to promote locomotor activity in this fly. The daily locomotor activity and the sleep patterns of ebony1 and tan1 mutants are analyzed, and are compared with wild-type flies. The tan1 mutant shows reduced locomotor activity, whereas ebony1 shows higher levels of activity than wild-type flies, suggesting that NBAD does not promote locomotor activity. Both mutants spend less time asleep than wild-type flies during night-time; ebony shows more consolidated activity during night-time and increased sleep latency, whereas tan is unable to consolidate locomotor activity and sleep in either phase of the day. The daily level of NBAD-synthase activity is measured in vitro using wild-type and tan1 protein extracts, and the lowest NBAD synthesis is observed at the time of higher locomotor activity. The abnormalities in several parameters of the waking/sleep cycle indicate some dysfunction in the processes that regulates these behaviours in both mutants.
publishDate 2015
dc.date.none.fl_str_mv 2015-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/14230
Rossi, Fabiana Alejandra; Bochicchio, Pablo Alejandro; Quesada Allue, Luis Alberto; Pérez, Martín Mariano; N-β-alanyldopamine metabolism, locomotor activity and sleep in Drosophila melanogaster ebony and tan mutants; John Wiley & Sons Inc; Physiological Entomology (print); 40; 2; 6-2015; 166-174
0307-6962
url http://hdl.handle.net/11336/14230
identifier_str_mv Rossi, Fabiana Alejandra; Bochicchio, Pablo Alejandro; Quesada Allue, Luis Alberto; Pérez, Martín Mariano; N-β-alanyldopamine metabolism, locomotor activity and sleep in Drosophila melanogaster ebony and tan mutants; John Wiley & Sons Inc; Physiological Entomology (print); 40; 2; 6-2015; 166-174
0307-6962
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/phen.12100/full
info:eu-repo/semantics/altIdentifier/doi/10.1111/phen.12100
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv John Wiley & Sons Inc
publisher.none.fl_str_mv John Wiley & Sons Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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