Bacterial community shifts in nonylphenol polyethoxylates-enriched activated sludge
- Autores
- Lozada, Mariana; Itria, Raúl Fabio; Figuerola, Eva Lucia Margarita; Babay, Paola Alejandra; Gettar, Raquel T.; De Tullio, Luis A.; Erijman, Leonardo
- Año de publicación
- 2004
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- A molecular approach was used to evaluate the effect of nonylphenol ethoxylate surfactants on the bacterial diversity in lab-scale activated sludge reactors. Separate bench-scale units were fed synthetic wastewater with and without addition of branched nonylphenol ethoxylates (NPnEO). The performance of the reactors, in terms of carbonaceous removal was largely unaffected by the presence of NP10EO in the feeding solution. However, addition of NP10EO exerted a pronounced shift in bacterial community composition. In situ hybridization analyzing larger phylogenetic groups of bacteria with ribosomal RNA-targeted oligonucleotide probes revealed the dominance of clusters composed of Betaproteobacteria, accounting for up to one-third of 4′,6-diamidino-2- phenylindol-dihydrochloride (DAPI)-stained cells in NP10EO amended reactors and only 5% of DAPI-stained cells in the controls. These shifts in populations of larger phylogenetic groups were confirmed by dot-blot analysis of rRNA. Members of gamma subclass of Proteobacteria were present in low numbers in all activated sludge samples examined, suggesting that only bacteria affiliated with the beta subclass of Proteobacteria may have a specific role in NP10EO degradation.
Fil: Lozada, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Itria, Raúl Fabio. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnologı́a Industrial; Argentina
Fil: Figuerola, Eva Lucia Margarita. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Babay, Paola Alejandra. Comisión Nacional de Energía Atómica; Argentina
Fil: Gettar, Raquel T.. Comisión Nacional de Energía Atómica; Argentina
Fil: De Tullio, Luis A.. Instituto Nacional de Tecnologı́a Industrial; Argentina
Fil: Erijman, Leonardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina - Materia
-
Activated Sludge
Betaproteobacteria
Community Structure
Fluorescence in Situ Hybridization
Nonionic Surfactant
Nonylphenol Ethoxylates - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/79774
Ver los metadatos del registro completo
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Bacterial community shifts in nonylphenol polyethoxylates-enriched activated sludgeLozada, MarianaItria, Raúl FabioFiguerola, Eva Lucia MargaritaBabay, Paola AlejandraGettar, Raquel T.De Tullio, Luis A.Erijman, LeonardoActivated SludgeBetaproteobacteriaCommunity StructureFluorescence in Situ HybridizationNonionic SurfactantNonylphenol Ethoxylateshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1A molecular approach was used to evaluate the effect of nonylphenol ethoxylate surfactants on the bacterial diversity in lab-scale activated sludge reactors. Separate bench-scale units were fed synthetic wastewater with and without addition of branched nonylphenol ethoxylates (NPnEO). The performance of the reactors, in terms of carbonaceous removal was largely unaffected by the presence of NP10EO in the feeding solution. However, addition of NP10EO exerted a pronounced shift in bacterial community composition. In situ hybridization analyzing larger phylogenetic groups of bacteria with ribosomal RNA-targeted oligonucleotide probes revealed the dominance of clusters composed of Betaproteobacteria, accounting for up to one-third of 4′,6-diamidino-2- phenylindol-dihydrochloride (DAPI)-stained cells in NP10EO amended reactors and only 5% of DAPI-stained cells in the controls. These shifts in populations of larger phylogenetic groups were confirmed by dot-blot analysis of rRNA. Members of gamma subclass of Proteobacteria were present in low numbers in all activated sludge samples examined, suggesting that only bacteria affiliated with the beta subclass of Proteobacteria may have a specific role in NP10EO degradation.Fil: Lozada, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Itria, Raúl Fabio. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnologı́a Industrial; ArgentinaFil: Figuerola, Eva Lucia Margarita. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Babay, Paola Alejandra. Comisión Nacional de Energía Atómica; ArgentinaFil: Gettar, Raquel T.. Comisión Nacional de Energía Atómica; ArgentinaFil: De Tullio, Luis A.. Instituto Nacional de Tecnologı́a Industrial; ArgentinaFil: Erijman, Leonardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaPergamon-Elsevier Science Ltd2004-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/79774Lozada, Mariana; Itria, Raúl Fabio; Figuerola, Eva Lucia Margarita; Babay, Paola Alejandra; Gettar, Raquel T.; et al.; Bacterial community shifts in nonylphenol polyethoxylates-enriched activated sludge; Pergamon-Elsevier Science Ltd; Water Research; 38; 8; 4-2004; 2077-20860043-1354CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.watres.2004.01.032info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pubmed/15087188info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0043135404000557?via%3Dihubinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:27:03Zoai:ri.conicet.gov.ar:11336/79774instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:27:04.134CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Bacterial community shifts in nonylphenol polyethoxylates-enriched activated sludge |
title |
Bacterial community shifts in nonylphenol polyethoxylates-enriched activated sludge |
spellingShingle |
Bacterial community shifts in nonylphenol polyethoxylates-enriched activated sludge Lozada, Mariana Activated Sludge Betaproteobacteria Community Structure Fluorescence in Situ Hybridization Nonionic Surfactant Nonylphenol Ethoxylates |
title_short |
Bacterial community shifts in nonylphenol polyethoxylates-enriched activated sludge |
title_full |
Bacterial community shifts in nonylphenol polyethoxylates-enriched activated sludge |
title_fullStr |
Bacterial community shifts in nonylphenol polyethoxylates-enriched activated sludge |
title_full_unstemmed |
Bacterial community shifts in nonylphenol polyethoxylates-enriched activated sludge |
title_sort |
Bacterial community shifts in nonylphenol polyethoxylates-enriched activated sludge |
dc.creator.none.fl_str_mv |
Lozada, Mariana Itria, Raúl Fabio Figuerola, Eva Lucia Margarita Babay, Paola Alejandra Gettar, Raquel T. De Tullio, Luis A. Erijman, Leonardo |
author |
Lozada, Mariana |
author_facet |
Lozada, Mariana Itria, Raúl Fabio Figuerola, Eva Lucia Margarita Babay, Paola Alejandra Gettar, Raquel T. De Tullio, Luis A. Erijman, Leonardo |
author_role |
author |
author2 |
Itria, Raúl Fabio Figuerola, Eva Lucia Margarita Babay, Paola Alejandra Gettar, Raquel T. De Tullio, Luis A. Erijman, Leonardo |
author2_role |
author author author author author author |
dc.subject.none.fl_str_mv |
Activated Sludge Betaproteobacteria Community Structure Fluorescence in Situ Hybridization Nonionic Surfactant Nonylphenol Ethoxylates |
topic |
Activated Sludge Betaproteobacteria Community Structure Fluorescence in Situ Hybridization Nonionic Surfactant Nonylphenol Ethoxylates |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
A molecular approach was used to evaluate the effect of nonylphenol ethoxylate surfactants on the bacterial diversity in lab-scale activated sludge reactors. Separate bench-scale units were fed synthetic wastewater with and without addition of branched nonylphenol ethoxylates (NPnEO). The performance of the reactors, in terms of carbonaceous removal was largely unaffected by the presence of NP10EO in the feeding solution. However, addition of NP10EO exerted a pronounced shift in bacterial community composition. In situ hybridization analyzing larger phylogenetic groups of bacteria with ribosomal RNA-targeted oligonucleotide probes revealed the dominance of clusters composed of Betaproteobacteria, accounting for up to one-third of 4′,6-diamidino-2- phenylindol-dihydrochloride (DAPI)-stained cells in NP10EO amended reactors and only 5% of DAPI-stained cells in the controls. These shifts in populations of larger phylogenetic groups were confirmed by dot-blot analysis of rRNA. Members of gamma subclass of Proteobacteria were present in low numbers in all activated sludge samples examined, suggesting that only bacteria affiliated with the beta subclass of Proteobacteria may have a specific role in NP10EO degradation. Fil: Lozada, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina Fil: Itria, Raúl Fabio. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnologı́a Industrial; Argentina Fil: Figuerola, Eva Lucia Margarita. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina Fil: Babay, Paola Alejandra. Comisión Nacional de Energía Atómica; Argentina Fil: Gettar, Raquel T.. Comisión Nacional de Energía Atómica; Argentina Fil: De Tullio, Luis A.. Instituto Nacional de Tecnologı́a Industrial; Argentina Fil: Erijman, Leonardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina |
description |
A molecular approach was used to evaluate the effect of nonylphenol ethoxylate surfactants on the bacterial diversity in lab-scale activated sludge reactors. Separate bench-scale units were fed synthetic wastewater with and without addition of branched nonylphenol ethoxylates (NPnEO). The performance of the reactors, in terms of carbonaceous removal was largely unaffected by the presence of NP10EO in the feeding solution. However, addition of NP10EO exerted a pronounced shift in bacterial community composition. In situ hybridization analyzing larger phylogenetic groups of bacteria with ribosomal RNA-targeted oligonucleotide probes revealed the dominance of clusters composed of Betaproteobacteria, accounting for up to one-third of 4′,6-diamidino-2- phenylindol-dihydrochloride (DAPI)-stained cells in NP10EO amended reactors and only 5% of DAPI-stained cells in the controls. These shifts in populations of larger phylogenetic groups were confirmed by dot-blot analysis of rRNA. Members of gamma subclass of Proteobacteria were present in low numbers in all activated sludge samples examined, suggesting that only bacteria affiliated with the beta subclass of Proteobacteria may have a specific role in NP10EO degradation. |
publishDate |
2004 |
dc.date.none.fl_str_mv |
2004-04 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/79774 Lozada, Mariana; Itria, Raúl Fabio; Figuerola, Eva Lucia Margarita; Babay, Paola Alejandra; Gettar, Raquel T.; et al.; Bacterial community shifts in nonylphenol polyethoxylates-enriched activated sludge; Pergamon-Elsevier Science Ltd; Water Research; 38; 8; 4-2004; 2077-2086 0043-1354 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/79774 |
identifier_str_mv |
Lozada, Mariana; Itria, Raúl Fabio; Figuerola, Eva Lucia Margarita; Babay, Paola Alejandra; Gettar, Raquel T.; et al.; Bacterial community shifts in nonylphenol polyethoxylates-enriched activated sludge; Pergamon-Elsevier Science Ltd; Water Research; 38; 8; 4-2004; 2077-2086 0043-1354 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.watres.2004.01.032 info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pubmed/15087188 info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0043135404000557?via%3Dihub |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Pergamon-Elsevier Science Ltd |
publisher.none.fl_str_mv |
Pergamon-Elsevier Science Ltd |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) |
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CONICET Digital (CONICET) |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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13.070432 |