Bacterial community shifts in nonylphenol polyethoxylates-enriched activated sludge

Autores
Lozada, Mariana; Itria, Raúl Fabio; Figuerola, Eva Lucia Margarita; Babay, Paola Alejandra; Gettar, Raquel T.; De Tullio, Luis A.; Erijman, Leonardo
Año de publicación
2004
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A molecular approach was used to evaluate the effect of nonylphenol ethoxylate surfactants on the bacterial diversity in lab-scale activated sludge reactors. Separate bench-scale units were fed synthetic wastewater with and without addition of branched nonylphenol ethoxylates (NPnEO). The performance of the reactors, in terms of carbonaceous removal was largely unaffected by the presence of NP10EO in the feeding solution. However, addition of NP10EO exerted a pronounced shift in bacterial community composition. In situ hybridization analyzing larger phylogenetic groups of bacteria with ribosomal RNA-targeted oligonucleotide probes revealed the dominance of clusters composed of Betaproteobacteria, accounting for up to one-third of 4′,6-diamidino-2- phenylindol-dihydrochloride (DAPI)-stained cells in NP10EO amended reactors and only 5% of DAPI-stained cells in the controls. These shifts in populations of larger phylogenetic groups were confirmed by dot-blot analysis of rRNA. Members of gamma subclass of Proteobacteria were present in low numbers in all activated sludge samples examined, suggesting that only bacteria affiliated with the beta subclass of Proteobacteria may have a specific role in NP10EO degradation.
Fil: Lozada, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Itria, Raúl Fabio. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnologı́a Industrial; Argentina
Fil: Figuerola, Eva Lucia Margarita. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Babay, Paola Alejandra. Comisión Nacional de Energía Atómica; Argentina
Fil: Gettar, Raquel T.. Comisión Nacional de Energía Atómica; Argentina
Fil: De Tullio, Luis A.. Instituto Nacional de Tecnologı́a Industrial; Argentina
Fil: Erijman, Leonardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Materia
Activated Sludge
Betaproteobacteria
Community Structure
Fluorescence in Situ Hybridization
Nonionic Surfactant
Nonylphenol Ethoxylates
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/79774
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Bacterial community shifts in nonylphenol polyethoxylates-enriched activated sludgeLozada, MarianaItria, Raúl FabioFiguerola, Eva Lucia MargaritaBabay, Paola AlejandraGettar, Raquel T.De Tullio, Luis A.Erijman, LeonardoActivated SludgeBetaproteobacteriaCommunity StructureFluorescence in Situ HybridizationNonionic SurfactantNonylphenol Ethoxylateshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1A molecular approach was used to evaluate the effect of nonylphenol ethoxylate surfactants on the bacterial diversity in lab-scale activated sludge reactors. Separate bench-scale units were fed synthetic wastewater with and without addition of branched nonylphenol ethoxylates (NPnEO). The performance of the reactors, in terms of carbonaceous removal was largely unaffected by the presence of NP10EO in the feeding solution. However, addition of NP10EO exerted a pronounced shift in bacterial community composition. In situ hybridization analyzing larger phylogenetic groups of bacteria with ribosomal RNA-targeted oligonucleotide probes revealed the dominance of clusters composed of Betaproteobacteria, accounting for up to one-third of 4′,6-diamidino-2- phenylindol-dihydrochloride (DAPI)-stained cells in NP10EO amended reactors and only 5% of DAPI-stained cells in the controls. These shifts in populations of larger phylogenetic groups were confirmed by dot-blot analysis of rRNA. Members of gamma subclass of Proteobacteria were present in low numbers in all activated sludge samples examined, suggesting that only bacteria affiliated with the beta subclass of Proteobacteria may have a specific role in NP10EO degradation.Fil: Lozada, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Itria, Raúl Fabio. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnologı́a Industrial; ArgentinaFil: Figuerola, Eva Lucia Margarita. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Babay, Paola Alejandra. Comisión Nacional de Energía Atómica; ArgentinaFil: Gettar, Raquel T.. Comisión Nacional de Energía Atómica; ArgentinaFil: De Tullio, Luis A.. Instituto Nacional de Tecnologı́a Industrial; ArgentinaFil: Erijman, Leonardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaPergamon-Elsevier Science Ltd2004-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/79774Lozada, Mariana; Itria, Raúl Fabio; Figuerola, Eva Lucia Margarita; Babay, Paola Alejandra; Gettar, Raquel T.; et al.; Bacterial community shifts in nonylphenol polyethoxylates-enriched activated sludge; Pergamon-Elsevier Science Ltd; Water Research; 38; 8; 4-2004; 2077-20860043-1354CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.watres.2004.01.032info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pubmed/15087188info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0043135404000557?via%3Dihubinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:24:04Zoai:ri.conicet.gov.ar:11336/79774instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:24:05.047CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Bacterial community shifts in nonylphenol polyethoxylates-enriched activated sludge
title Bacterial community shifts in nonylphenol polyethoxylates-enriched activated sludge
spellingShingle Bacterial community shifts in nonylphenol polyethoxylates-enriched activated sludge
Lozada, Mariana
Activated Sludge
Betaproteobacteria
Community Structure
Fluorescence in Situ Hybridization
Nonionic Surfactant
Nonylphenol Ethoxylates
title_short Bacterial community shifts in nonylphenol polyethoxylates-enriched activated sludge
title_full Bacterial community shifts in nonylphenol polyethoxylates-enriched activated sludge
title_fullStr Bacterial community shifts in nonylphenol polyethoxylates-enriched activated sludge
title_full_unstemmed Bacterial community shifts in nonylphenol polyethoxylates-enriched activated sludge
title_sort Bacterial community shifts in nonylphenol polyethoxylates-enriched activated sludge
dc.creator.none.fl_str_mv Lozada, Mariana
Itria, Raúl Fabio
Figuerola, Eva Lucia Margarita
Babay, Paola Alejandra
Gettar, Raquel T.
De Tullio, Luis A.
Erijman, Leonardo
author Lozada, Mariana
author_facet Lozada, Mariana
Itria, Raúl Fabio
Figuerola, Eva Lucia Margarita
Babay, Paola Alejandra
Gettar, Raquel T.
De Tullio, Luis A.
Erijman, Leonardo
author_role author
author2 Itria, Raúl Fabio
Figuerola, Eva Lucia Margarita
Babay, Paola Alejandra
Gettar, Raquel T.
De Tullio, Luis A.
Erijman, Leonardo
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Activated Sludge
Betaproteobacteria
Community Structure
Fluorescence in Situ Hybridization
Nonionic Surfactant
Nonylphenol Ethoxylates
topic Activated Sludge
Betaproteobacteria
Community Structure
Fluorescence in Situ Hybridization
Nonionic Surfactant
Nonylphenol Ethoxylates
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv A molecular approach was used to evaluate the effect of nonylphenol ethoxylate surfactants on the bacterial diversity in lab-scale activated sludge reactors. Separate bench-scale units were fed synthetic wastewater with and without addition of branched nonylphenol ethoxylates (NPnEO). The performance of the reactors, in terms of carbonaceous removal was largely unaffected by the presence of NP10EO in the feeding solution. However, addition of NP10EO exerted a pronounced shift in bacterial community composition. In situ hybridization analyzing larger phylogenetic groups of bacteria with ribosomal RNA-targeted oligonucleotide probes revealed the dominance of clusters composed of Betaproteobacteria, accounting for up to one-third of 4′,6-diamidino-2- phenylindol-dihydrochloride (DAPI)-stained cells in NP10EO amended reactors and only 5% of DAPI-stained cells in the controls. These shifts in populations of larger phylogenetic groups were confirmed by dot-blot analysis of rRNA. Members of gamma subclass of Proteobacteria were present in low numbers in all activated sludge samples examined, suggesting that only bacteria affiliated with the beta subclass of Proteobacteria may have a specific role in NP10EO degradation.
Fil: Lozada, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Itria, Raúl Fabio. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnologı́a Industrial; Argentina
Fil: Figuerola, Eva Lucia Margarita. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Babay, Paola Alejandra. Comisión Nacional de Energía Atómica; Argentina
Fil: Gettar, Raquel T.. Comisión Nacional de Energía Atómica; Argentina
Fil: De Tullio, Luis A.. Instituto Nacional de Tecnologı́a Industrial; Argentina
Fil: Erijman, Leonardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
description A molecular approach was used to evaluate the effect of nonylphenol ethoxylate surfactants on the bacterial diversity in lab-scale activated sludge reactors. Separate bench-scale units were fed synthetic wastewater with and without addition of branched nonylphenol ethoxylates (NPnEO). The performance of the reactors, in terms of carbonaceous removal was largely unaffected by the presence of NP10EO in the feeding solution. However, addition of NP10EO exerted a pronounced shift in bacterial community composition. In situ hybridization analyzing larger phylogenetic groups of bacteria with ribosomal RNA-targeted oligonucleotide probes revealed the dominance of clusters composed of Betaproteobacteria, accounting for up to one-third of 4′,6-diamidino-2- phenylindol-dihydrochloride (DAPI)-stained cells in NP10EO amended reactors and only 5% of DAPI-stained cells in the controls. These shifts in populations of larger phylogenetic groups were confirmed by dot-blot analysis of rRNA. Members of gamma subclass of Proteobacteria were present in low numbers in all activated sludge samples examined, suggesting that only bacteria affiliated with the beta subclass of Proteobacteria may have a specific role in NP10EO degradation.
publishDate 2004
dc.date.none.fl_str_mv 2004-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/79774
Lozada, Mariana; Itria, Raúl Fabio; Figuerola, Eva Lucia Margarita; Babay, Paola Alejandra; Gettar, Raquel T.; et al.; Bacterial community shifts in nonylphenol polyethoxylates-enriched activated sludge; Pergamon-Elsevier Science Ltd; Water Research; 38; 8; 4-2004; 2077-2086
0043-1354
CONICET Digital
CONICET
url http://hdl.handle.net/11336/79774
identifier_str_mv Lozada, Mariana; Itria, Raúl Fabio; Figuerola, Eva Lucia Margarita; Babay, Paola Alejandra; Gettar, Raquel T.; et al.; Bacterial community shifts in nonylphenol polyethoxylates-enriched activated sludge; Pergamon-Elsevier Science Ltd; Water Research; 38; 8; 4-2004; 2077-2086
0043-1354
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.watres.2004.01.032
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pubmed/15087188
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0043135404000557?via%3Dihub
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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