β1-Adrenoceptor antibody-induced increase in soluble CD40 ligand release in chronic periodontitis patients: Role of prostaglandin E2
- Autores
- Sterin Borda, Leonor; Segovia, Marcela; Reina, Silvia Lorena; Borda, Enri Santiago
- Año de publicación
- 2012
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- In this paper, we demonstrate that circulating antibodies from chronic periodontitis patients reacting with atrial β1-adrenoceptors (β1-ARs) act as an inducer of soluble CD40 ligand (sCD40L) release and prostaglandin E2 (PGE2) generation. By enzyme-linked immunosorbent assay using β1 synthetic peptide (with an amino acid sequence identical to the second loop of human myocardial β1-ARs) as a coating antigen, we demonstrated reactivity against the second extracellular loop on human myocardial β1-ARs. This autoantibody present in the serum of chronic periodontitis patients was significantly correlated with the release of sCD40L and PGE2. The release of sCD40L was blunted by atenolol, SP600125 and β1 synthetic peptide, and PGE2 generation was inhibited by DuP 697 and slightly by FR122049. The effects of the antibody incubated with isolated rat atria upregulated sCD40L release with an increase of PGE2 production and c-Jun N-terminal kinase phosphorylation. These results indicate that in chronic periodontitis patients, there is a positive association between sCD40L release and PGE2 generation via the action of β1-AR antibodies.
Fil: Sterin Borda, Leonor. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Farmacología; Argentina
Fil: Segovia, Marcela. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Farmacología; Argentina
Fil: Reina, Silvia Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Farmacología; Argentina
Fil: Borda, Enri Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Farmacología; Argentina - Materia
-
CD 40 LINGAND
PROSTAGLANDIN
ADRENORECEPTOR ANTIBODY
JNK - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/216459
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β1-Adrenoceptor antibody-induced increase in soluble CD40 ligand release in chronic periodontitis patients: Role of prostaglandin E2Sterin Borda, LeonorSegovia, MarcelaReina, Silvia LorenaBorda, Enri SantiagoCD 40 LINGANDPROSTAGLANDINADRENORECEPTOR ANTIBODYJNKhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3In this paper, we demonstrate that circulating antibodies from chronic periodontitis patients reacting with atrial β1-adrenoceptors (β1-ARs) act as an inducer of soluble CD40 ligand (sCD40L) release and prostaglandin E2 (PGE2) generation. By enzyme-linked immunosorbent assay using β1 synthetic peptide (with an amino acid sequence identical to the second loop of human myocardial β1-ARs) as a coating antigen, we demonstrated reactivity against the second extracellular loop on human myocardial β1-ARs. This autoantibody present in the serum of chronic periodontitis patients was significantly correlated with the release of sCD40L and PGE2. The release of sCD40L was blunted by atenolol, SP600125 and β1 synthetic peptide, and PGE2 generation was inhibited by DuP 697 and slightly by FR122049. The effects of the antibody incubated with isolated rat atria upregulated sCD40L release with an increase of PGE2 production and c-Jun N-terminal kinase phosphorylation. These results indicate that in chronic periodontitis patients, there is a positive association between sCD40L release and PGE2 generation via the action of β1-AR antibodies.Fil: Sterin Borda, Leonor. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Farmacología; ArgentinaFil: Segovia, Marcela. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Farmacología; ArgentinaFil: Reina, Silvia Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Farmacología; ArgentinaFil: Borda, Enri Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Farmacología; ArgentinaWiley Blackwell Publishing, Inc2012-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/216459Sterin Borda, Leonor; Segovia, Marcela; Reina, Silvia Lorena; Borda, Enri Santiago; β1-Adrenoceptor antibody-induced increase in soluble CD40 ligand release in chronic periodontitis patients: Role of prostaglandin E2; Wiley Blackwell Publishing, Inc; Experimental Physiology; 97; 9; 4-2012; 1030-10390958-0670CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://physoc.onlinelibrary.wiley.com/doi/full/10.1113/expphysiol.2012.065748info:eu-repo/semantics/altIdentifier/doi/10.1113/expphysiol.2012.065748info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:32:28Zoai:ri.conicet.gov.ar:11336/216459instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:32:28.636CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
β1-Adrenoceptor antibody-induced increase in soluble CD40 ligand release in chronic periodontitis patients: Role of prostaglandin E2 |
title |
β1-Adrenoceptor antibody-induced increase in soluble CD40 ligand release in chronic periodontitis patients: Role of prostaglandin E2 |
spellingShingle |
β1-Adrenoceptor antibody-induced increase in soluble CD40 ligand release in chronic periodontitis patients: Role of prostaglandin E2 Sterin Borda, Leonor CD 40 LINGAND PROSTAGLANDIN ADRENORECEPTOR ANTIBODY JNK |
title_short |
β1-Adrenoceptor antibody-induced increase in soluble CD40 ligand release in chronic periodontitis patients: Role of prostaglandin E2 |
title_full |
β1-Adrenoceptor antibody-induced increase in soluble CD40 ligand release in chronic periodontitis patients: Role of prostaglandin E2 |
title_fullStr |
β1-Adrenoceptor antibody-induced increase in soluble CD40 ligand release in chronic periodontitis patients: Role of prostaglandin E2 |
title_full_unstemmed |
β1-Adrenoceptor antibody-induced increase in soluble CD40 ligand release in chronic periodontitis patients: Role of prostaglandin E2 |
title_sort |
β1-Adrenoceptor antibody-induced increase in soluble CD40 ligand release in chronic periodontitis patients: Role of prostaglandin E2 |
dc.creator.none.fl_str_mv |
Sterin Borda, Leonor Segovia, Marcela Reina, Silvia Lorena Borda, Enri Santiago |
author |
Sterin Borda, Leonor |
author_facet |
Sterin Borda, Leonor Segovia, Marcela Reina, Silvia Lorena Borda, Enri Santiago |
author_role |
author |
author2 |
Segovia, Marcela Reina, Silvia Lorena Borda, Enri Santiago |
author2_role |
author author author |
dc.subject.none.fl_str_mv |
CD 40 LINGAND PROSTAGLANDIN ADRENORECEPTOR ANTIBODY JNK |
topic |
CD 40 LINGAND PROSTAGLANDIN ADRENORECEPTOR ANTIBODY JNK |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/3.3 https://purl.org/becyt/ford/3 |
dc.description.none.fl_txt_mv |
In this paper, we demonstrate that circulating antibodies from chronic periodontitis patients reacting with atrial β1-adrenoceptors (β1-ARs) act as an inducer of soluble CD40 ligand (sCD40L) release and prostaglandin E2 (PGE2) generation. By enzyme-linked immunosorbent assay using β1 synthetic peptide (with an amino acid sequence identical to the second loop of human myocardial β1-ARs) as a coating antigen, we demonstrated reactivity against the second extracellular loop on human myocardial β1-ARs. This autoantibody present in the serum of chronic periodontitis patients was significantly correlated with the release of sCD40L and PGE2. The release of sCD40L was blunted by atenolol, SP600125 and β1 synthetic peptide, and PGE2 generation was inhibited by DuP 697 and slightly by FR122049. The effects of the antibody incubated with isolated rat atria upregulated sCD40L release with an increase of PGE2 production and c-Jun N-terminal kinase phosphorylation. These results indicate that in chronic periodontitis patients, there is a positive association between sCD40L release and PGE2 generation via the action of β1-AR antibodies. Fil: Sterin Borda, Leonor. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Farmacología; Argentina Fil: Segovia, Marcela. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Farmacología; Argentina Fil: Reina, Silvia Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Farmacología; Argentina Fil: Borda, Enri Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Farmacología; Argentina |
description |
In this paper, we demonstrate that circulating antibodies from chronic periodontitis patients reacting with atrial β1-adrenoceptors (β1-ARs) act as an inducer of soluble CD40 ligand (sCD40L) release and prostaglandin E2 (PGE2) generation. By enzyme-linked immunosorbent assay using β1 synthetic peptide (with an amino acid sequence identical to the second loop of human myocardial β1-ARs) as a coating antigen, we demonstrated reactivity against the second extracellular loop on human myocardial β1-ARs. This autoantibody present in the serum of chronic periodontitis patients was significantly correlated with the release of sCD40L and PGE2. The release of sCD40L was blunted by atenolol, SP600125 and β1 synthetic peptide, and PGE2 generation was inhibited by DuP 697 and slightly by FR122049. The effects of the antibody incubated with isolated rat atria upregulated sCD40L release with an increase of PGE2 production and c-Jun N-terminal kinase phosphorylation. These results indicate that in chronic periodontitis patients, there is a positive association between sCD40L release and PGE2 generation via the action of β1-AR antibodies. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-04 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/216459 Sterin Borda, Leonor; Segovia, Marcela; Reina, Silvia Lorena; Borda, Enri Santiago; β1-Adrenoceptor antibody-induced increase in soluble CD40 ligand release in chronic periodontitis patients: Role of prostaglandin E2; Wiley Blackwell Publishing, Inc; Experimental Physiology; 97; 9; 4-2012; 1030-1039 0958-0670 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/216459 |
identifier_str_mv |
Sterin Borda, Leonor; Segovia, Marcela; Reina, Silvia Lorena; Borda, Enri Santiago; β1-Adrenoceptor antibody-induced increase in soluble CD40 ligand release in chronic periodontitis patients: Role of prostaglandin E2; Wiley Blackwell Publishing, Inc; Experimental Physiology; 97; 9; 4-2012; 1030-1039 0958-0670 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://physoc.onlinelibrary.wiley.com/doi/full/10.1113/expphysiol.2012.065748 info:eu-repo/semantics/altIdentifier/doi/10.1113/expphysiol.2012.065748 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Wiley Blackwell Publishing, Inc |
publisher.none.fl_str_mv |
Wiley Blackwell Publishing, Inc |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844612990185242624 |
score |
13.070432 |