Distribution across tissue layers of extrinsic nerves innervating the mouse colorectum – An in vitro anterograde tracing study

Autores
Brumovsky, Pablo Rodolfo; La, Jun Ho; Gebhart, Gerald F.
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background Anterograde in vitro tracing of the pelvic nerve (PN) and visualization in the horizontal plane in whole mount preparations has been fundamental in the analysis of distribution of peripheral nerves innervating the colorectum. Here, we performed a similar analysis, but in cryostat sections of the mouse colorectum, allowing for a more direct visualization of nerve distribution in all tissue layers. Methods Colorectum with attached PNs was dissected from adult male BalbC mice. Presence of active afferents was certified by single fiber recording of fine PN fibers. This was followed by ‘bulk’ (all fibers) anterograde tracing using biotinamide (BTA). Histo- and immunohistochemical techniques were used for visualization of BTA-positive nerves, and evaluation of co-localization with calcitonin gene-related peptide (CGRP), respectively. Tissue was analyzed using confocal microscopy on transverse or longitudinal colorectum sections. Key Results Abundant BTA-positive nerves spanning all layers of the mouse colorectum and contacting myenteric plexus neurons, distributing within the muscle layer, penetrating deeper into the organ and contacting blood vessels, submucosal plexus neurons or even penetrating the mucosa, were regularly detected. Several traced axons co-localized CGRP, supporting their afferent nature. Finally, anterograde tracing of the PN also exposed abundant BTA-positive nerves in the major pelvic ganglion. Conclusions & Inferences We present the patterns of innervation of extrinsic axons across layers in the mouse colorectum, including the labile mucosal layer. The proposed approach could also be useful in the analysis of associations between morphology and physiology of peripheral nerves targeting the different layers of the colorectum.
Fil: Brumovsky, Pablo Rodolfo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Austral; Argentina. University of Pittsburgh; Estados Unidos
Fil: La, Jun Ho. University of Pittsburgh; Estados Unidos
Fil: Gebhart, Gerald F.. University of Pittsburgh; Estados Unidos
Materia
ANTEROGRADE
BIOTINAMIDE
COLORECTUM
IN VITRO TRACING
PERIPHERAL NERVES
PRIMARY AFFERENTS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/35936

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network_name_str CONICET Digital (CONICET)
spelling Distribution across tissue layers of extrinsic nerves innervating the mouse colorectum – An in vitro anterograde tracing studyBrumovsky, Pablo RodolfoLa, Jun HoGebhart, Gerald F.ANTEROGRADEBIOTINAMIDECOLORECTUMIN VITRO TRACINGPERIPHERAL NERVESPRIMARY AFFERENTShttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Background Anterograde in vitro tracing of the pelvic nerve (PN) and visualization in the horizontal plane in whole mount preparations has been fundamental in the analysis of distribution of peripheral nerves innervating the colorectum. Here, we performed a similar analysis, but in cryostat sections of the mouse colorectum, allowing for a more direct visualization of nerve distribution in all tissue layers. Methods Colorectum with attached PNs was dissected from adult male BalbC mice. Presence of active afferents was certified by single fiber recording of fine PN fibers. This was followed by ‘bulk’ (all fibers) anterograde tracing using biotinamide (BTA). Histo- and immunohistochemical techniques were used for visualization of BTA-positive nerves, and evaluation of co-localization with calcitonin gene-related peptide (CGRP), respectively. Tissue was analyzed using confocal microscopy on transverse or longitudinal colorectum sections. Key Results Abundant BTA-positive nerves spanning all layers of the mouse colorectum and contacting myenteric plexus neurons, distributing within the muscle layer, penetrating deeper into the organ and contacting blood vessels, submucosal plexus neurons or even penetrating the mucosa, were regularly detected. Several traced axons co-localized CGRP, supporting their afferent nature. Finally, anterograde tracing of the PN also exposed abundant BTA-positive nerves in the major pelvic ganglion. Conclusions & Inferences We present the patterns of innervation of extrinsic axons across layers in the mouse colorectum, including the labile mucosal layer. The proposed approach could also be useful in the analysis of associations between morphology and physiology of peripheral nerves targeting the different layers of the colorectum.Fil: Brumovsky, Pablo Rodolfo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Austral; Argentina. University of Pittsburgh; Estados UnidosFil: La, Jun Ho. University of Pittsburgh; Estados UnidosFil: Gebhart, Gerald F.. University of Pittsburgh; Estados UnidosWiley Blackwell Publishing, Inc2014-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/35936Brumovsky, Pablo Rodolfo; La, Jun Ho; Gebhart, Gerald F.; Distribution across tissue layers of extrinsic nerves innervating the mouse colorectum – An in vitro anterograde tracing study; Wiley Blackwell Publishing, Inc; Neurogastroenterology And Motility; 26; 10; 10-2014; 1494-15071350-1925CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1111/nmo.12419info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/nmo.12419/abstractinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:56:07Zoai:ri.conicet.gov.ar:11336/35936instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:56:07.548CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Distribution across tissue layers of extrinsic nerves innervating the mouse colorectum – An in vitro anterograde tracing study
title Distribution across tissue layers of extrinsic nerves innervating the mouse colorectum – An in vitro anterograde tracing study
spellingShingle Distribution across tissue layers of extrinsic nerves innervating the mouse colorectum – An in vitro anterograde tracing study
Brumovsky, Pablo Rodolfo
ANTEROGRADE
BIOTINAMIDE
COLORECTUM
IN VITRO TRACING
PERIPHERAL NERVES
PRIMARY AFFERENTS
title_short Distribution across tissue layers of extrinsic nerves innervating the mouse colorectum – An in vitro anterograde tracing study
title_full Distribution across tissue layers of extrinsic nerves innervating the mouse colorectum – An in vitro anterograde tracing study
title_fullStr Distribution across tissue layers of extrinsic nerves innervating the mouse colorectum – An in vitro anterograde tracing study
title_full_unstemmed Distribution across tissue layers of extrinsic nerves innervating the mouse colorectum – An in vitro anterograde tracing study
title_sort Distribution across tissue layers of extrinsic nerves innervating the mouse colorectum – An in vitro anterograde tracing study
dc.creator.none.fl_str_mv Brumovsky, Pablo Rodolfo
La, Jun Ho
Gebhart, Gerald F.
author Brumovsky, Pablo Rodolfo
author_facet Brumovsky, Pablo Rodolfo
La, Jun Ho
Gebhart, Gerald F.
author_role author
author2 La, Jun Ho
Gebhart, Gerald F.
author2_role author
author
dc.subject.none.fl_str_mv ANTEROGRADE
BIOTINAMIDE
COLORECTUM
IN VITRO TRACING
PERIPHERAL NERVES
PRIMARY AFFERENTS
topic ANTEROGRADE
BIOTINAMIDE
COLORECTUM
IN VITRO TRACING
PERIPHERAL NERVES
PRIMARY AFFERENTS
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Background Anterograde in vitro tracing of the pelvic nerve (PN) and visualization in the horizontal plane in whole mount preparations has been fundamental in the analysis of distribution of peripheral nerves innervating the colorectum. Here, we performed a similar analysis, but in cryostat sections of the mouse colorectum, allowing for a more direct visualization of nerve distribution in all tissue layers. Methods Colorectum with attached PNs was dissected from adult male BalbC mice. Presence of active afferents was certified by single fiber recording of fine PN fibers. This was followed by ‘bulk’ (all fibers) anterograde tracing using biotinamide (BTA). Histo- and immunohistochemical techniques were used for visualization of BTA-positive nerves, and evaluation of co-localization with calcitonin gene-related peptide (CGRP), respectively. Tissue was analyzed using confocal microscopy on transverse or longitudinal colorectum sections. Key Results Abundant BTA-positive nerves spanning all layers of the mouse colorectum and contacting myenteric plexus neurons, distributing within the muscle layer, penetrating deeper into the organ and contacting blood vessels, submucosal plexus neurons or even penetrating the mucosa, were regularly detected. Several traced axons co-localized CGRP, supporting their afferent nature. Finally, anterograde tracing of the PN also exposed abundant BTA-positive nerves in the major pelvic ganglion. Conclusions & Inferences We present the patterns of innervation of extrinsic axons across layers in the mouse colorectum, including the labile mucosal layer. The proposed approach could also be useful in the analysis of associations between morphology and physiology of peripheral nerves targeting the different layers of the colorectum.
Fil: Brumovsky, Pablo Rodolfo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Austral; Argentina. University of Pittsburgh; Estados Unidos
Fil: La, Jun Ho. University of Pittsburgh; Estados Unidos
Fil: Gebhart, Gerald F.. University of Pittsburgh; Estados Unidos
description Background Anterograde in vitro tracing of the pelvic nerve (PN) and visualization in the horizontal plane in whole mount preparations has been fundamental in the analysis of distribution of peripheral nerves innervating the colorectum. Here, we performed a similar analysis, but in cryostat sections of the mouse colorectum, allowing for a more direct visualization of nerve distribution in all tissue layers. Methods Colorectum with attached PNs was dissected from adult male BalbC mice. Presence of active afferents was certified by single fiber recording of fine PN fibers. This was followed by ‘bulk’ (all fibers) anterograde tracing using biotinamide (BTA). Histo- and immunohistochemical techniques were used for visualization of BTA-positive nerves, and evaluation of co-localization with calcitonin gene-related peptide (CGRP), respectively. Tissue was analyzed using confocal microscopy on transverse or longitudinal colorectum sections. Key Results Abundant BTA-positive nerves spanning all layers of the mouse colorectum and contacting myenteric plexus neurons, distributing within the muscle layer, penetrating deeper into the organ and contacting blood vessels, submucosal plexus neurons or even penetrating the mucosa, were regularly detected. Several traced axons co-localized CGRP, supporting their afferent nature. Finally, anterograde tracing of the PN also exposed abundant BTA-positive nerves in the major pelvic ganglion. Conclusions & Inferences We present the patterns of innervation of extrinsic axons across layers in the mouse colorectum, including the labile mucosal layer. The proposed approach could also be useful in the analysis of associations between morphology and physiology of peripheral nerves targeting the different layers of the colorectum.
publishDate 2014
dc.date.none.fl_str_mv 2014-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/35936
Brumovsky, Pablo Rodolfo; La, Jun Ho; Gebhart, Gerald F.; Distribution across tissue layers of extrinsic nerves innervating the mouse colorectum – An in vitro anterograde tracing study; Wiley Blackwell Publishing, Inc; Neurogastroenterology And Motility; 26; 10; 10-2014; 1494-1507
1350-1925
CONICET Digital
CONICET
url http://hdl.handle.net/11336/35936
identifier_str_mv Brumovsky, Pablo Rodolfo; La, Jun Ho; Gebhart, Gerald F.; Distribution across tissue layers of extrinsic nerves innervating the mouse colorectum – An in vitro anterograde tracing study; Wiley Blackwell Publishing, Inc; Neurogastroenterology And Motility; 26; 10; 10-2014; 1494-1507
1350-1925
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1111/nmo.12419
info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/nmo.12419/abstract
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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