Distribution across tissue layers of extrinsic nerves innervating the mouse colorectum – An in vitro anterograde tracing study
- Autores
- Brumovsky, Pablo Rodolfo; La, Jun Ho; Gebhart, Gerald F.
- Año de publicación
- 2014
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Background Anterograde in vitro tracing of the pelvic nerve (PN) and visualization in the horizontal plane in whole mount preparations has been fundamental in the analysis of distribution of peripheral nerves innervating the colorectum. Here, we performed a similar analysis, but in cryostat sections of the mouse colorectum, allowing for a more direct visualization of nerve distribution in all tissue layers. Methods Colorectum with attached PNs was dissected from adult male BalbC mice. Presence of active afferents was certified by single fiber recording of fine PN fibers. This was followed by ‘bulk’ (all fibers) anterograde tracing using biotinamide (BTA). Histo- and immunohistochemical techniques were used for visualization of BTA-positive nerves, and evaluation of co-localization with calcitonin gene-related peptide (CGRP), respectively. Tissue was analyzed using confocal microscopy on transverse or longitudinal colorectum sections. Key Results Abundant BTA-positive nerves spanning all layers of the mouse colorectum and contacting myenteric plexus neurons, distributing within the muscle layer, penetrating deeper into the organ and contacting blood vessels, submucosal plexus neurons or even penetrating the mucosa, were regularly detected. Several traced axons co-localized CGRP, supporting their afferent nature. Finally, anterograde tracing of the PN also exposed abundant BTA-positive nerves in the major pelvic ganglion. Conclusions & Inferences We present the patterns of innervation of extrinsic axons across layers in the mouse colorectum, including the labile mucosal layer. The proposed approach could also be useful in the analysis of associations between morphology and physiology of peripheral nerves targeting the different layers of the colorectum.
Fil: Brumovsky, Pablo Rodolfo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Austral; Argentina. University of Pittsburgh; Estados Unidos
Fil: La, Jun Ho. University of Pittsburgh; Estados Unidos
Fil: Gebhart, Gerald F.. University of Pittsburgh; Estados Unidos - Materia
-
ANTEROGRADE
BIOTINAMIDE
COLORECTUM
IN VITRO TRACING
PERIPHERAL NERVES
PRIMARY AFFERENTS - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/35936
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Distribution across tissue layers of extrinsic nerves innervating the mouse colorectum – An in vitro anterograde tracing studyBrumovsky, Pablo RodolfoLa, Jun HoGebhart, Gerald F.ANTEROGRADEBIOTINAMIDECOLORECTUMIN VITRO TRACINGPERIPHERAL NERVESPRIMARY AFFERENTShttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Background Anterograde in vitro tracing of the pelvic nerve (PN) and visualization in the horizontal plane in whole mount preparations has been fundamental in the analysis of distribution of peripheral nerves innervating the colorectum. Here, we performed a similar analysis, but in cryostat sections of the mouse colorectum, allowing for a more direct visualization of nerve distribution in all tissue layers. Methods Colorectum with attached PNs was dissected from adult male BalbC mice. Presence of active afferents was certified by single fiber recording of fine PN fibers. This was followed by ‘bulk’ (all fibers) anterograde tracing using biotinamide (BTA). Histo- and immunohistochemical techniques were used for visualization of BTA-positive nerves, and evaluation of co-localization with calcitonin gene-related peptide (CGRP), respectively. Tissue was analyzed using confocal microscopy on transverse or longitudinal colorectum sections. Key Results Abundant BTA-positive nerves spanning all layers of the mouse colorectum and contacting myenteric plexus neurons, distributing within the muscle layer, penetrating deeper into the organ and contacting blood vessels, submucosal plexus neurons or even penetrating the mucosa, were regularly detected. Several traced axons co-localized CGRP, supporting their afferent nature. Finally, anterograde tracing of the PN also exposed abundant BTA-positive nerves in the major pelvic ganglion. Conclusions & Inferences We present the patterns of innervation of extrinsic axons across layers in the mouse colorectum, including the labile mucosal layer. The proposed approach could also be useful in the analysis of associations between morphology and physiology of peripheral nerves targeting the different layers of the colorectum.Fil: Brumovsky, Pablo Rodolfo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Austral; Argentina. University of Pittsburgh; Estados UnidosFil: La, Jun Ho. University of Pittsburgh; Estados UnidosFil: Gebhart, Gerald F.. University of Pittsburgh; Estados UnidosWiley Blackwell Publishing, Inc2014-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/35936Brumovsky, Pablo Rodolfo; La, Jun Ho; Gebhart, Gerald F.; Distribution across tissue layers of extrinsic nerves innervating the mouse colorectum – An in vitro anterograde tracing study; Wiley Blackwell Publishing, Inc; Neurogastroenterology And Motility; 26; 10; 10-2014; 1494-15071350-1925CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1111/nmo.12419info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/nmo.12419/abstractinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:56:07Zoai:ri.conicet.gov.ar:11336/35936instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:56:07.548CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Distribution across tissue layers of extrinsic nerves innervating the mouse colorectum – An in vitro anterograde tracing study |
title |
Distribution across tissue layers of extrinsic nerves innervating the mouse colorectum – An in vitro anterograde tracing study |
spellingShingle |
Distribution across tissue layers of extrinsic nerves innervating the mouse colorectum – An in vitro anterograde tracing study Brumovsky, Pablo Rodolfo ANTEROGRADE BIOTINAMIDE COLORECTUM IN VITRO TRACING PERIPHERAL NERVES PRIMARY AFFERENTS |
title_short |
Distribution across tissue layers of extrinsic nerves innervating the mouse colorectum – An in vitro anterograde tracing study |
title_full |
Distribution across tissue layers of extrinsic nerves innervating the mouse colorectum – An in vitro anterograde tracing study |
title_fullStr |
Distribution across tissue layers of extrinsic nerves innervating the mouse colorectum – An in vitro anterograde tracing study |
title_full_unstemmed |
Distribution across tissue layers of extrinsic nerves innervating the mouse colorectum – An in vitro anterograde tracing study |
title_sort |
Distribution across tissue layers of extrinsic nerves innervating the mouse colorectum – An in vitro anterograde tracing study |
dc.creator.none.fl_str_mv |
Brumovsky, Pablo Rodolfo La, Jun Ho Gebhart, Gerald F. |
author |
Brumovsky, Pablo Rodolfo |
author_facet |
Brumovsky, Pablo Rodolfo La, Jun Ho Gebhart, Gerald F. |
author_role |
author |
author2 |
La, Jun Ho Gebhart, Gerald F. |
author2_role |
author author |
dc.subject.none.fl_str_mv |
ANTEROGRADE BIOTINAMIDE COLORECTUM IN VITRO TRACING PERIPHERAL NERVES PRIMARY AFFERENTS |
topic |
ANTEROGRADE BIOTINAMIDE COLORECTUM IN VITRO TRACING PERIPHERAL NERVES PRIMARY AFFERENTS |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/3.1 https://purl.org/becyt/ford/3 |
dc.description.none.fl_txt_mv |
Background Anterograde in vitro tracing of the pelvic nerve (PN) and visualization in the horizontal plane in whole mount preparations has been fundamental in the analysis of distribution of peripheral nerves innervating the colorectum. Here, we performed a similar analysis, but in cryostat sections of the mouse colorectum, allowing for a more direct visualization of nerve distribution in all tissue layers. Methods Colorectum with attached PNs was dissected from adult male BalbC mice. Presence of active afferents was certified by single fiber recording of fine PN fibers. This was followed by ‘bulk’ (all fibers) anterograde tracing using biotinamide (BTA). Histo- and immunohistochemical techniques were used for visualization of BTA-positive nerves, and evaluation of co-localization with calcitonin gene-related peptide (CGRP), respectively. Tissue was analyzed using confocal microscopy on transverse or longitudinal colorectum sections. Key Results Abundant BTA-positive nerves spanning all layers of the mouse colorectum and contacting myenteric plexus neurons, distributing within the muscle layer, penetrating deeper into the organ and contacting blood vessels, submucosal plexus neurons or even penetrating the mucosa, were regularly detected. Several traced axons co-localized CGRP, supporting their afferent nature. Finally, anterograde tracing of the PN also exposed abundant BTA-positive nerves in the major pelvic ganglion. Conclusions & Inferences We present the patterns of innervation of extrinsic axons across layers in the mouse colorectum, including the labile mucosal layer. The proposed approach could also be useful in the analysis of associations between morphology and physiology of peripheral nerves targeting the different layers of the colorectum. Fil: Brumovsky, Pablo Rodolfo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Austral; Argentina. University of Pittsburgh; Estados Unidos Fil: La, Jun Ho. University of Pittsburgh; Estados Unidos Fil: Gebhart, Gerald F.. University of Pittsburgh; Estados Unidos |
description |
Background Anterograde in vitro tracing of the pelvic nerve (PN) and visualization in the horizontal plane in whole mount preparations has been fundamental in the analysis of distribution of peripheral nerves innervating the colorectum. Here, we performed a similar analysis, but in cryostat sections of the mouse colorectum, allowing for a more direct visualization of nerve distribution in all tissue layers. Methods Colorectum with attached PNs was dissected from adult male BalbC mice. Presence of active afferents was certified by single fiber recording of fine PN fibers. This was followed by ‘bulk’ (all fibers) anterograde tracing using biotinamide (BTA). Histo- and immunohistochemical techniques were used for visualization of BTA-positive nerves, and evaluation of co-localization with calcitonin gene-related peptide (CGRP), respectively. Tissue was analyzed using confocal microscopy on transverse or longitudinal colorectum sections. Key Results Abundant BTA-positive nerves spanning all layers of the mouse colorectum and contacting myenteric plexus neurons, distributing within the muscle layer, penetrating deeper into the organ and contacting blood vessels, submucosal plexus neurons or even penetrating the mucosa, were regularly detected. Several traced axons co-localized CGRP, supporting their afferent nature. Finally, anterograde tracing of the PN also exposed abundant BTA-positive nerves in the major pelvic ganglion. Conclusions & Inferences We present the patterns of innervation of extrinsic axons across layers in the mouse colorectum, including the labile mucosal layer. The proposed approach could also be useful in the analysis of associations between morphology and physiology of peripheral nerves targeting the different layers of the colorectum. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-10 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/35936 Brumovsky, Pablo Rodolfo; La, Jun Ho; Gebhart, Gerald F.; Distribution across tissue layers of extrinsic nerves innervating the mouse colorectum – An in vitro anterograde tracing study; Wiley Blackwell Publishing, Inc; Neurogastroenterology And Motility; 26; 10; 10-2014; 1494-1507 1350-1925 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/35936 |
identifier_str_mv |
Brumovsky, Pablo Rodolfo; La, Jun Ho; Gebhart, Gerald F.; Distribution across tissue layers of extrinsic nerves innervating the mouse colorectum – An in vitro anterograde tracing study; Wiley Blackwell Publishing, Inc; Neurogastroenterology And Motility; 26; 10; 10-2014; 1494-1507 1350-1925 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1111/nmo.12419 info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/nmo.12419/abstract |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Wiley Blackwell Publishing, Inc |
publisher.none.fl_str_mv |
Wiley Blackwell Publishing, Inc |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844613688218091520 |
score |
13.070432 |