Expression of vesicular glutamate transporters type 1 and 2 in sensory and autonomic neurons innervating the mouse colorectum

Autores
Brumovsky, Pablo Rodolfo; Robinson, David R.; La, Jun Ho; Seroogy, Kim B.; Lundgren, Kerstin H.; Albers, Kathryn M.; Kiyatkin, Michael E.; Seal, Rebecca P.; Edwards, Robert H.; Watanabe, Masahiko; Hökfelt, Tomas; Gebhart, G. F.
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Vesicular glutamate transporters (VGLUTs) have been extensively studied in various neuronal systems, but their expression in visceral sensory and autonomic neurons remains to be analyzed in detail. Here we studied VGLUTs type 1 and 2 (VGLUT(1) and VGLUT(2) , respectively) in neurons innervating the mouse colorectum. Lumbosacral and thoracolumbar dorsal root ganglion (DRG), lumbar sympathetic chain (LSC), and major pelvic ganglion (MPG) neurons innervating the colorectum of BALB/C mice were retrogradely traced with Fast Blue, dissected, and processed for immunohistochemistry. Tissue from additional naïve mice was included. Previously characterized antibodies against VGLUT(1) , VGLUT(2) , and calcitonin gene-related peptide (CGRP) were used. Riboprobe in situ hybridization, using probes against VGLUT(1) and VGLUT(2) , was also performed. Most colorectal DRG neurons expressed VGLUT(2) and often colocalized with CGRP. A smaller percentage of neurons expressed VGLUT(1) . VGLUT(2) -immunoreactive (IR) neurons in the MPG were rare. Abundant VGLUT(2) -IR nerves were detected in all layers of the colorectum; VGLUT(1) -IR nerves were sparse. A subpopulation of myenteric plexus neurons expressed VGLUT2 protein and mRNA, but VGLUT1 mRNA was undetectable. In conclusion, we show 1) that most colorectal DRG neurons express VGLUT(2) , and to a lesser extent, VGLUT(1) ; 2) abundance of VGLUT2-IR fibers innervating colorectum; and 3) a subpopulation of myenteric plexus neurons expressing VGLUT(2). Altogether, our data suggests a role for VGLUT(2) in colorectal glutamatergic neurotransmission, potentially influencing colorectal sensitivity and motility.
Fil: Brumovsky, Pablo Rodolfo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Univeristy of Pittsburgh. School of Medicine; Estados Unidos. Universidad Austral; Argentina
Fil: Robinson, David R.. Univeristy of Pittsburgh. School of Medicine; Estados Unidos. University of Pittsburgh at Johnstown; Estados Unidos. University of Pittsburgh; Estados Unidos
Fil: La, Jun Ho. Univeristy of Pittsburgh. School of Medicine; Estados Unidos
Fil: Seroogy, Kim B.. No especifíca;
Fil: Lundgren, Kerstin H.. No especifíca;
Fil: Albers, Kathryn M.. Univeristy of Pittsburgh. School of Medicine; Estados Unidos
Fil: Kiyatkin, Michael E.. Univeristy of Pittsburgh. School of Medicine; Estados Unidos
Fil: Seal, Rebecca P.. No especifíca;
Fil: Edwards, Robert H.. No especifíca;
Fil: Watanabe, Masahiko. No especifíca;
Fil: Hökfelt, Tomas. Karolinska Huddinge Hospital. Karolinska Institutet; Suecia
Fil: Gebhart, G. F.. Univeristy of Pittsburgh. School of Medicine; Estados Unidos
Materia
COLON
DRG
GLUTAMATE
NEUROPEPTIDES
PAIN
PELVIC NERVE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/193812
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/193812
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Expression of vesicular glutamate transporters type 1 and 2 in sensory and autonomic neurons innervating the mouse colorectumBrumovsky, Pablo RodolfoRobinson, David R.La, Jun HoSeroogy, Kim B.Lundgren, Kerstin H.Albers, Kathryn M.Kiyatkin, Michael E.Seal, Rebecca P.Edwards, Robert H.Watanabe, MasahikoHökfelt, TomasGebhart, G. F.COLONDRGGLUTAMATENEUROPEPTIDESPAINPELVIC NERVEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Vesicular glutamate transporters (VGLUTs) have been extensively studied in various neuronal systems, but their expression in visceral sensory and autonomic neurons remains to be analyzed in detail. Here we studied VGLUTs type 1 and 2 (VGLUT(1) and VGLUT(2) , respectively) in neurons innervating the mouse colorectum. Lumbosacral and thoracolumbar dorsal root ganglion (DRG), lumbar sympathetic chain (LSC), and major pelvic ganglion (MPG) neurons innervating the colorectum of BALB/C mice were retrogradely traced with Fast Blue, dissected, and processed for immunohistochemistry. Tissue from additional naïve mice was included. Previously characterized antibodies against VGLUT(1) , VGLUT(2) , and calcitonin gene-related peptide (CGRP) were used. Riboprobe in situ hybridization, using probes against VGLUT(1) and VGLUT(2) , was also performed. Most colorectal DRG neurons expressed VGLUT(2) and often colocalized with CGRP. A smaller percentage of neurons expressed VGLUT(1) . VGLUT(2) -immunoreactive (IR) neurons in the MPG were rare. Abundant VGLUT(2) -IR nerves were detected in all layers of the colorectum; VGLUT(1) -IR nerves were sparse. A subpopulation of myenteric plexus neurons expressed VGLUT2 protein and mRNA, but VGLUT1 mRNA was undetectable. In conclusion, we show 1) that most colorectal DRG neurons express VGLUT(2) , and to a lesser extent, VGLUT(1) ; 2) abundance of VGLUT2-IR fibers innervating colorectum; and 3) a subpopulation of myenteric plexus neurons expressing VGLUT(2). Altogether, our data suggests a role for VGLUT(2) in colorectal glutamatergic neurotransmission, potentially influencing colorectal sensitivity and motility.Fil: Brumovsky, Pablo Rodolfo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Univeristy of Pittsburgh. School of Medicine; Estados Unidos. Universidad Austral; ArgentinaFil: Robinson, David R.. Univeristy of Pittsburgh. School of Medicine; Estados Unidos. University of Pittsburgh at Johnstown; Estados Unidos. University of Pittsburgh; Estados UnidosFil: La, Jun Ho. Univeristy of Pittsburgh. School of Medicine; Estados UnidosFil: Seroogy, Kim B.. No especifíca;Fil: Lundgren, Kerstin H.. No especifíca;Fil: Albers, Kathryn M.. Univeristy of Pittsburgh. School of Medicine; Estados UnidosFil: Kiyatkin, Michael E.. Univeristy of Pittsburgh. School of Medicine; Estados UnidosFil: Seal, Rebecca P.. No especifíca;Fil: Edwards, Robert H.. No especifíca;Fil: Watanabe, Masahiko. No especifíca;Fil: Hökfelt, Tomas. Karolinska Huddinge Hospital. Karolinska Institutet; SueciaFil: Gebhart, G. F.. Univeristy of Pittsburgh. School of Medicine; Estados UnidosWiley-liss, div John Wiley & Sons Inc.2011-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/193812Brumovsky, Pablo Rodolfo; Robinson, David R.; La, Jun Ho; Seroogy, Kim B.; Lundgren, Kerstin H.; et al.; Expression of vesicular glutamate transporters type 1 and 2 in sensory and autonomic neurons innervating the mouse colorectum; Wiley-liss, div John Wiley & Sons Inc.; Journal Of Comparative Neurology; 519; 16; 11-2011; 3346-33660021-9967CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1002/cne.22730info:eu-repo/semantics/altIdentifier/doi/10.1002/cne.22730info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:37:13Zoai:ri.conicet.gov.ar:11336/193812instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:37:13.928CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Expression of vesicular glutamate transporters type 1 and 2 in sensory and autonomic neurons innervating the mouse colorectum
title Expression of vesicular glutamate transporters type 1 and 2 in sensory and autonomic neurons innervating the mouse colorectum
spellingShingle Expression of vesicular glutamate transporters type 1 and 2 in sensory and autonomic neurons innervating the mouse colorectum
Brumovsky, Pablo Rodolfo
COLON
DRG
GLUTAMATE
NEUROPEPTIDES
PAIN
PELVIC NERVE
title_short Expression of vesicular glutamate transporters type 1 and 2 in sensory and autonomic neurons innervating the mouse colorectum
title_full Expression of vesicular glutamate transporters type 1 and 2 in sensory and autonomic neurons innervating the mouse colorectum
title_fullStr Expression of vesicular glutamate transporters type 1 and 2 in sensory and autonomic neurons innervating the mouse colorectum
title_full_unstemmed Expression of vesicular glutamate transporters type 1 and 2 in sensory and autonomic neurons innervating the mouse colorectum
title_sort Expression of vesicular glutamate transporters type 1 and 2 in sensory and autonomic neurons innervating the mouse colorectum
dc.creator.none.fl_str_mv Brumovsky, Pablo Rodolfo
Robinson, David R.
La, Jun Ho
Seroogy, Kim B.
Lundgren, Kerstin H.
Albers, Kathryn M.
Kiyatkin, Michael E.
Seal, Rebecca P.
Edwards, Robert H.
Watanabe, Masahiko
Hökfelt, Tomas
Gebhart, G. F.
author Brumovsky, Pablo Rodolfo
author_facet Brumovsky, Pablo Rodolfo
Robinson, David R.
La, Jun Ho
Seroogy, Kim B.
Lundgren, Kerstin H.
Albers, Kathryn M.
Kiyatkin, Michael E.
Seal, Rebecca P.
Edwards, Robert H.
Watanabe, Masahiko
Hökfelt, Tomas
Gebhart, G. F.
author_role author
author2 Robinson, David R.
La, Jun Ho
Seroogy, Kim B.
Lundgren, Kerstin H.
Albers, Kathryn M.
Kiyatkin, Michael E.
Seal, Rebecca P.
Edwards, Robert H.
Watanabe, Masahiko
Hökfelt, Tomas
Gebhart, G. F.
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv COLON
DRG
GLUTAMATE
NEUROPEPTIDES
PAIN
PELVIC NERVE
topic COLON
DRG
GLUTAMATE
NEUROPEPTIDES
PAIN
PELVIC NERVE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Vesicular glutamate transporters (VGLUTs) have been extensively studied in various neuronal systems, but their expression in visceral sensory and autonomic neurons remains to be analyzed in detail. Here we studied VGLUTs type 1 and 2 (VGLUT(1) and VGLUT(2) , respectively) in neurons innervating the mouse colorectum. Lumbosacral and thoracolumbar dorsal root ganglion (DRG), lumbar sympathetic chain (LSC), and major pelvic ganglion (MPG) neurons innervating the colorectum of BALB/C mice were retrogradely traced with Fast Blue, dissected, and processed for immunohistochemistry. Tissue from additional naïve mice was included. Previously characterized antibodies against VGLUT(1) , VGLUT(2) , and calcitonin gene-related peptide (CGRP) were used. Riboprobe in situ hybridization, using probes against VGLUT(1) and VGLUT(2) , was also performed. Most colorectal DRG neurons expressed VGLUT(2) and often colocalized with CGRP. A smaller percentage of neurons expressed VGLUT(1) . VGLUT(2) -immunoreactive (IR) neurons in the MPG were rare. Abundant VGLUT(2) -IR nerves were detected in all layers of the colorectum; VGLUT(1) -IR nerves were sparse. A subpopulation of myenteric plexus neurons expressed VGLUT2 protein and mRNA, but VGLUT1 mRNA was undetectable. In conclusion, we show 1) that most colorectal DRG neurons express VGLUT(2) , and to a lesser extent, VGLUT(1) ; 2) abundance of VGLUT2-IR fibers innervating colorectum; and 3) a subpopulation of myenteric plexus neurons expressing VGLUT(2). Altogether, our data suggests a role for VGLUT(2) in colorectal glutamatergic neurotransmission, potentially influencing colorectal sensitivity and motility.
Fil: Brumovsky, Pablo Rodolfo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Univeristy of Pittsburgh. School of Medicine; Estados Unidos. Universidad Austral; Argentina
Fil: Robinson, David R.. Univeristy of Pittsburgh. School of Medicine; Estados Unidos. University of Pittsburgh at Johnstown; Estados Unidos. University of Pittsburgh; Estados Unidos
Fil: La, Jun Ho. Univeristy of Pittsburgh. School of Medicine; Estados Unidos
Fil: Seroogy, Kim B.. No especifíca;
Fil: Lundgren, Kerstin H.. No especifíca;
Fil: Albers, Kathryn M.. Univeristy of Pittsburgh. School of Medicine; Estados Unidos
Fil: Kiyatkin, Michael E.. Univeristy of Pittsburgh. School of Medicine; Estados Unidos
Fil: Seal, Rebecca P.. No especifíca;
Fil: Edwards, Robert H.. No especifíca;
Fil: Watanabe, Masahiko. No especifíca;
Fil: Hökfelt, Tomas. Karolinska Huddinge Hospital. Karolinska Institutet; Suecia
Fil: Gebhart, G. F.. Univeristy of Pittsburgh. School of Medicine; Estados Unidos
description Vesicular glutamate transporters (VGLUTs) have been extensively studied in various neuronal systems, but their expression in visceral sensory and autonomic neurons remains to be analyzed in detail. Here we studied VGLUTs type 1 and 2 (VGLUT(1) and VGLUT(2) , respectively) in neurons innervating the mouse colorectum. Lumbosacral and thoracolumbar dorsal root ganglion (DRG), lumbar sympathetic chain (LSC), and major pelvic ganglion (MPG) neurons innervating the colorectum of BALB/C mice were retrogradely traced with Fast Blue, dissected, and processed for immunohistochemistry. Tissue from additional naïve mice was included. Previously characterized antibodies against VGLUT(1) , VGLUT(2) , and calcitonin gene-related peptide (CGRP) were used. Riboprobe in situ hybridization, using probes against VGLUT(1) and VGLUT(2) , was also performed. Most colorectal DRG neurons expressed VGLUT(2) and often colocalized with CGRP. A smaller percentage of neurons expressed VGLUT(1) . VGLUT(2) -immunoreactive (IR) neurons in the MPG were rare. Abundant VGLUT(2) -IR nerves were detected in all layers of the colorectum; VGLUT(1) -IR nerves were sparse. A subpopulation of myenteric plexus neurons expressed VGLUT2 protein and mRNA, but VGLUT1 mRNA was undetectable. In conclusion, we show 1) that most colorectal DRG neurons express VGLUT(2) , and to a lesser extent, VGLUT(1) ; 2) abundance of VGLUT2-IR fibers innervating colorectum; and 3) a subpopulation of myenteric plexus neurons expressing VGLUT(2). Altogether, our data suggests a role for VGLUT(2) in colorectal glutamatergic neurotransmission, potentially influencing colorectal sensitivity and motility.
publishDate 2011
dc.date.none.fl_str_mv 2011-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/193812
Brumovsky, Pablo Rodolfo; Robinson, David R.; La, Jun Ho; Seroogy, Kim B.; Lundgren, Kerstin H.; et al.; Expression of vesicular glutamate transporters type 1 and 2 in sensory and autonomic neurons innervating the mouse colorectum; Wiley-liss, div John Wiley & Sons Inc.; Journal Of Comparative Neurology; 519; 16; 11-2011; 3346-3366
0021-9967
CONICET Digital
CONICET
url http://hdl.handle.net/11336/193812
identifier_str_mv Brumovsky, Pablo Rodolfo; Robinson, David R.; La, Jun Ho; Seroogy, Kim B.; Lundgren, Kerstin H.; et al.; Expression of vesicular glutamate transporters type 1 and 2 in sensory and autonomic neurons innervating the mouse colorectum; Wiley-liss, div John Wiley & Sons Inc.; Journal Of Comparative Neurology; 519; 16; 11-2011; 3346-3366
0021-9967
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1002/cne.22730
info:eu-repo/semantics/altIdentifier/doi/10.1002/cne.22730
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley-liss, div John Wiley & Sons Inc.
publisher.none.fl_str_mv Wiley-liss, div John Wiley & Sons Inc.
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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