Exitianus obscurinervis (Hemiptera: Cicadellidae), a New Experimental Vector of Spiroplasma kunkelii

Autores
Carloni, Edgardo José; Virla, Eduardo Gabriel; Paradell, Susana Liria; Carpane, Pablo D.; Nome Docampo, Claudia; Laguna, Irma Graciela; Gimenez, Maria De La Paz
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
“Corn stunt” caused by the mollicute Spiroplasma kunkelii (Whitcomb) is potentially one of the most severe diseases affecting the corn (Zea mays L.) crop in the Americas, and the leafhopper Dalbulus maidis (DeLong & Wolcott) is considered its most important vector. However, other insects seen quite frequently in corn crops might well be its vectors in Argentina. To identify any leafhoppers species other than D. maidis that can transmit S. kunkelii, transmission assays were conducted, using individuals of Exitianus obscurinervis (Stål) collected in field and reared under controlled conditions. S. kunkelii was transmitted to corn plants by E. obscurinervis. The pathogen was transmitted to seven of the 11 plants, which showed characteristic corn stunt symptoms, and the presence of the pathogen was confirmed by DAS-ELISA. The presence of S. kunkelii in the E. obscurinervis individuals used in transmission experiments was confirmed by polymerase chain reaction and electron microscopy. The current study shows the existence of a new experimental vector of S. kunkelii, the leafhopper E. obscurinervis, which acquired spiroplasmas from infected plants and inoculated it to healthy plants.
Instituto de Fisiología y Recursos Genéticos Vegetales
Fil: Carloni, Edgardo José. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fil: Carloni, Edgardo José. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA); Argentina
Fil: Virla, E. Consejo Nacional de Investigaciones Científicas y Técnicas. Planta Piloto de Procesos Industriales Microbiológicos (PROIMI); Argentina.
Fil: Paradell, S. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. División Entomología; Argentina
Fil: Carpane, P. Monsanto Argentina (SAIC); Argentina
Fil: Nome Docampo, Claudia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Nome Docampo, Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
Fil: Laguna, Irma Graciela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Gimenez, Maria De La Paz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Gimenez, Maria De La Paz. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
Fuente
Journal of Economic Entomology 104 (6) : 1793-1799 (December 2011)
Materia
Dalbulus maidis
Spiroplasma
Vectores
Vectors
Hemiptera
Corn Leafhopper
Corn Stunt
Exitianus obscurinervis
Spiroplasma kunkelii
Nivel de accesibilidad
acceso restringido
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/23893
Acceder
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oai_identifier_str oai:localhost:20.500.12123/23893
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spelling Exitianus obscurinervis (Hemiptera: Cicadellidae), a New Experimental Vector of Spiroplasma kunkeliiCarloni, Edgardo JoséVirla, Eduardo GabrielParadell, Susana LiriaCarpane, Pablo D.Nome Docampo, ClaudiaLaguna, Irma GracielaGimenez, Maria De La PazDalbulus maidisSpiroplasmaVectoresVectorsHemipteraCorn LeafhopperCorn StuntExitianus obscurinervisSpiroplasma kunkelii“Corn stunt” caused by the mollicute Spiroplasma kunkelii (Whitcomb) is potentially one of the most severe diseases affecting the corn (Zea mays L.) crop in the Americas, and the leafhopper Dalbulus maidis (DeLong & Wolcott) is considered its most important vector. However, other insects seen quite frequently in corn crops might well be its vectors in Argentina. To identify any leafhoppers species other than D. maidis that can transmit S. kunkelii, transmission assays were conducted, using individuals of Exitianus obscurinervis (Stål) collected in field and reared under controlled conditions. S. kunkelii was transmitted to corn plants by E. obscurinervis. The pathogen was transmitted to seven of the 11 plants, which showed characteristic corn stunt symptoms, and the presence of the pathogen was confirmed by DAS-ELISA. The presence of S. kunkelii in the E. obscurinervis individuals used in transmission experiments was confirmed by polymerase chain reaction and electron microscopy. The current study shows the existence of a new experimental vector of S. kunkelii, the leafhopper E. obscurinervis, which acquired spiroplasmas from infected plants and inoculated it to healthy plants.Instituto de Fisiología y Recursos Genéticos VegetalesFil: Carloni, Edgardo José. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; ArgentinaFil: Carloni, Edgardo José. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA); ArgentinaFil: Virla, E. Consejo Nacional de Investigaciones Científicas y Técnicas. Planta Piloto de Procesos Industriales Microbiológicos (PROIMI); Argentina.Fil: Paradell, S. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. División Entomología; ArgentinaFil: Carpane, P. Monsanto Argentina (SAIC); ArgentinaFil: Nome Docampo, Claudia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Nome Docampo, Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); ArgentinaFil: Laguna, Irma Graciela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Gimenez, Maria De La Paz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Gimenez, Maria De La Paz. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); ArgentinaOxford University Press2025-09-23T10:44:38Z2025-09-23T10:44:38Z2011-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/23893https://academic.oup.com/jee/article-abstract/104/6/1793/21997510022-04931938-291Xhttps://doi.org/10.1603/EC11156Journal of Economic Entomology 104 (6) : 1793-1799 (December 2011)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-29T13:47:32Zoai:localhost:20.500.12123/23893instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:47:33.101INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Exitianus obscurinervis (Hemiptera: Cicadellidae), a New Experimental Vector of Spiroplasma kunkelii
title Exitianus obscurinervis (Hemiptera: Cicadellidae), a New Experimental Vector of Spiroplasma kunkelii
spellingShingle Exitianus obscurinervis (Hemiptera: Cicadellidae), a New Experimental Vector of Spiroplasma kunkelii
Carloni, Edgardo José
Dalbulus maidis
Spiroplasma
Vectores
Vectors
Hemiptera
Corn Leafhopper
Corn Stunt
Exitianus obscurinervis
Spiroplasma kunkelii
title_short Exitianus obscurinervis (Hemiptera: Cicadellidae), a New Experimental Vector of Spiroplasma kunkelii
title_full Exitianus obscurinervis (Hemiptera: Cicadellidae), a New Experimental Vector of Spiroplasma kunkelii
title_fullStr Exitianus obscurinervis (Hemiptera: Cicadellidae), a New Experimental Vector of Spiroplasma kunkelii
title_full_unstemmed Exitianus obscurinervis (Hemiptera: Cicadellidae), a New Experimental Vector of Spiroplasma kunkelii
title_sort Exitianus obscurinervis (Hemiptera: Cicadellidae), a New Experimental Vector of Spiroplasma kunkelii
dc.creator.none.fl_str_mv Carloni, Edgardo José
Virla, Eduardo Gabriel
Paradell, Susana Liria
Carpane, Pablo D.
Nome Docampo, Claudia
Laguna, Irma Graciela
Gimenez, Maria De La Paz
author Carloni, Edgardo José
author_facet Carloni, Edgardo José
Virla, Eduardo Gabriel
Paradell, Susana Liria
Carpane, Pablo D.
Nome Docampo, Claudia
Laguna, Irma Graciela
Gimenez, Maria De La Paz
author_role author
author2 Virla, Eduardo Gabriel
Paradell, Susana Liria
Carpane, Pablo D.
Nome Docampo, Claudia
Laguna, Irma Graciela
Gimenez, Maria De La Paz
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Dalbulus maidis
Spiroplasma
Vectores
Vectors
Hemiptera
Corn Leafhopper
Corn Stunt
Exitianus obscurinervis
Spiroplasma kunkelii
topic Dalbulus maidis
Spiroplasma
Vectores
Vectors
Hemiptera
Corn Leafhopper
Corn Stunt
Exitianus obscurinervis
Spiroplasma kunkelii
dc.description.none.fl_txt_mv “Corn stunt” caused by the mollicute Spiroplasma kunkelii (Whitcomb) is potentially one of the most severe diseases affecting the corn (Zea mays L.) crop in the Americas, and the leafhopper Dalbulus maidis (DeLong & Wolcott) is considered its most important vector. However, other insects seen quite frequently in corn crops might well be its vectors in Argentina. To identify any leafhoppers species other than D. maidis that can transmit S. kunkelii, transmission assays were conducted, using individuals of Exitianus obscurinervis (Stål) collected in field and reared under controlled conditions. S. kunkelii was transmitted to corn plants by E. obscurinervis. The pathogen was transmitted to seven of the 11 plants, which showed characteristic corn stunt symptoms, and the presence of the pathogen was confirmed by DAS-ELISA. The presence of S. kunkelii in the E. obscurinervis individuals used in transmission experiments was confirmed by polymerase chain reaction and electron microscopy. The current study shows the existence of a new experimental vector of S. kunkelii, the leafhopper E. obscurinervis, which acquired spiroplasmas from infected plants and inoculated it to healthy plants.
Instituto de Fisiología y Recursos Genéticos Vegetales
Fil: Carloni, Edgardo José. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fil: Carloni, Edgardo José. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA); Argentina
Fil: Virla, E. Consejo Nacional de Investigaciones Científicas y Técnicas. Planta Piloto de Procesos Industriales Microbiológicos (PROIMI); Argentina.
Fil: Paradell, S. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. División Entomología; Argentina
Fil: Carpane, P. Monsanto Argentina (SAIC); Argentina
Fil: Nome Docampo, Claudia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Nome Docampo, Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
Fil: Laguna, Irma Graciela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Gimenez, Maria De La Paz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Gimenez, Maria De La Paz. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
description “Corn stunt” caused by the mollicute Spiroplasma kunkelii (Whitcomb) is potentially one of the most severe diseases affecting the corn (Zea mays L.) crop in the Americas, and the leafhopper Dalbulus maidis (DeLong & Wolcott) is considered its most important vector. However, other insects seen quite frequently in corn crops might well be its vectors in Argentina. To identify any leafhoppers species other than D. maidis that can transmit S. kunkelii, transmission assays were conducted, using individuals of Exitianus obscurinervis (Stål) collected in field and reared under controlled conditions. S. kunkelii was transmitted to corn plants by E. obscurinervis. The pathogen was transmitted to seven of the 11 plants, which showed characteristic corn stunt symptoms, and the presence of the pathogen was confirmed by DAS-ELISA. The presence of S. kunkelii in the E. obscurinervis individuals used in transmission experiments was confirmed by polymerase chain reaction and electron microscopy. The current study shows the existence of a new experimental vector of S. kunkelii, the leafhopper E. obscurinervis, which acquired spiroplasmas from infected plants and inoculated it to healthy plants.
publishDate 2011
dc.date.none.fl_str_mv 2011-12-01
2025-09-23T10:44:38Z
2025-09-23T10:44:38Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/23893
https://academic.oup.com/jee/article-abstract/104/6/1793/2199751
0022-0493
1938-291X
https://doi.org/10.1603/EC11156
url http://hdl.handle.net/20.500.12123/23893
https://academic.oup.com/jee/article-abstract/104/6/1793/2199751
https://doi.org/10.1603/EC11156
identifier_str_mv 0022-0493
1938-291X
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv restrictedAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv Journal of Economic Entomology 104 (6) : 1793-1799 (December 2011)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 13.069144

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