A Single Administration of the GnRH Antagonist Acyline Inhibits Basal and GnRH-Stimulated Serum Testosterone Concentrations in Male Dogs

Autores
Garcia Romero, Guadalupe; Mattioli, Guillermo Alberto; Rosa, Diana Esther; Díaz, Jorge Daniel; Abeyá, María Mercedes; Gobello, María Cristina
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The objective of this study was to describe testosterone (T) response to GnRH challenge in antagonist-treated dogs over a 30-day period. Eight mongrel dogs were randomly assigned to either the GnRH antagonist acyline 330μg/kg sc (ACY; n=4) or a placebo group (PLA; n=4). The dogs were serially challenged with the GnRH agonist, buserelin 0.2μg/kg sc on days -1, 1, 3, 7, 10, 14, 21 and 30. On these days, blood samples for T determinations were collected before (-30min) and 60, 120 and 180min after the agonist injection. Basal (-30min) and post-GnRH agonist stimulation T values were compared by anova for repeated measures. Before treatments (day -1), there were no differences in basal T serum concentrations between groups (p>0.1). After treatments, basal T showed a significant interaction between treatment and day (p<0.05). Furthermore, when both groups were analysed independently, basal T varied in the ACY (p<0.01) but not in the PLA group (p>0.1). On day -1, before treatments, the stimulation tests had only a time effect (p=0.05) although on days 1 (p<0.01), 3 (p<0.01), 7 (p<0.01), 10 (p<0.01) and 14 (p<0.05), the response to the agonist differed between groups, becoming similar on days 21 (p>0.05) and 30 (p>0.05). It was concluded that, in dogs, a single administration of the GnRH antagonist prevented canine gonadal axis to physiologically respond to agonistic challenge during 14 days.
Fil: Garcia Romero, Guadalupe. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Ciencias Básicas. Laboratorio de Nutrición Mineral y Fisiología Reproductiva; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Mattioli, Guillermo Alberto. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Ciencias Básicas. Laboratorio de Nutrición Mineral y Fisiología Reproductiva; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Rosa, Diana Esther. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Ciencias Básicas. Laboratorio de Nutrición Mineral y Fisiología Reproductiva; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Díaz, Jorge Daniel. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Ciencias Básicas. Laboratorio de Nutrición Mineral y Fisiología Reproductiva; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Abeyá, María Mercedes. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Ciencias Básicas. Laboratorio de Nutrición Mineral y Fisiología Reproductiva; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gobello, María Cristina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Ciencias Básicas. Laboratorio de Nutrición Mineral y Fisiología Reproductiva; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
Gnrh
Acyline
Testoterone
Dog
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/75355
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/75355
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling A Single Administration of the GnRH Antagonist Acyline Inhibits Basal and GnRH-Stimulated Serum Testosterone Concentrations in Male DogsGarcia Romero, GuadalupeMattioli, Guillermo AlbertoRosa, Diana EstherDíaz, Jorge DanielAbeyá, María MercedesGobello, María CristinaGnrhAcylineTestoteroneDoghttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4The objective of this study was to describe testosterone (T) response to GnRH challenge in antagonist-treated dogs over a 30-day period. Eight mongrel dogs were randomly assigned to either the GnRH antagonist acyline 330μg/kg sc (ACY; n=4) or a placebo group (PLA; n=4). The dogs were serially challenged with the GnRH agonist, buserelin 0.2μg/kg sc on days -1, 1, 3, 7, 10, 14, 21 and 30. On these days, blood samples for T determinations were collected before (-30min) and 60, 120 and 180min after the agonist injection. Basal (-30min) and post-GnRH agonist stimulation T values were compared by anova for repeated measures. Before treatments (day -1), there were no differences in basal T serum concentrations between groups (p>0.1). After treatments, basal T showed a significant interaction between treatment and day (p<0.05). Furthermore, when both groups were analysed independently, basal T varied in the ACY (p<0.01) but not in the PLA group (p>0.1). On day -1, before treatments, the stimulation tests had only a time effect (p=0.05) although on days 1 (p<0.01), 3 (p<0.01), 7 (p<0.01), 10 (p<0.01) and 14 (p<0.05), the response to the agonist differed between groups, becoming similar on days 21 (p>0.05) and 30 (p>0.05). It was concluded that, in dogs, a single administration of the GnRH antagonist prevented canine gonadal axis to physiologically respond to agonistic challenge during 14 days.Fil: Garcia Romero, Guadalupe. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Ciencias Básicas. Laboratorio de Nutrición Mineral y Fisiología Reproductiva; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Mattioli, Guillermo Alberto. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Ciencias Básicas. Laboratorio de Nutrición Mineral y Fisiología Reproductiva; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Rosa, Diana Esther. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Ciencias Básicas. Laboratorio de Nutrición Mineral y Fisiología Reproductiva; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Díaz, Jorge Daniel. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Ciencias Básicas. Laboratorio de Nutrición Mineral y Fisiología Reproductiva; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Abeyá, María Mercedes. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Ciencias Básicas. Laboratorio de Nutrición Mineral y Fisiología Reproductiva; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Gobello, María Cristina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Ciencias Básicas. Laboratorio de Nutrición Mineral y Fisiología Reproductiva; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaWiley Blackwell Publishing, Inc2012-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/75355Garcia Romero, Guadalupe; Mattioli, Guillermo Alberto; Rosa, Diana Esther; Díaz, Jorge Daniel; Abeyá, María Mercedes; et al.; A Single Administration of the GnRH Antagonist Acyline Inhibits Basal and GnRH-Stimulated Serum Testosterone Concentrations in Male Dogs; Wiley Blackwell Publishing, Inc; Reproduction in Domestic Animals; 47; 3; 6-20120936-6768CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1111/j.1439-0531.2011.01898.xinfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1439-0531.2011.01898.xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-17T11:47:38Zoai:ri.conicet.gov.ar:11336/75355instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-17 11:47:38.78CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv A Single Administration of the GnRH Antagonist Acyline Inhibits Basal and GnRH-Stimulated Serum Testosterone Concentrations in Male Dogs
title A Single Administration of the GnRH Antagonist Acyline Inhibits Basal and GnRH-Stimulated Serum Testosterone Concentrations in Male Dogs
spellingShingle A Single Administration of the GnRH Antagonist Acyline Inhibits Basal and GnRH-Stimulated Serum Testosterone Concentrations in Male Dogs
Garcia Romero, Guadalupe
Gnrh
Acyline
Testoterone
Dog
title_short A Single Administration of the GnRH Antagonist Acyline Inhibits Basal and GnRH-Stimulated Serum Testosterone Concentrations in Male Dogs
title_full A Single Administration of the GnRH Antagonist Acyline Inhibits Basal and GnRH-Stimulated Serum Testosterone Concentrations in Male Dogs
title_fullStr A Single Administration of the GnRH Antagonist Acyline Inhibits Basal and GnRH-Stimulated Serum Testosterone Concentrations in Male Dogs
title_full_unstemmed A Single Administration of the GnRH Antagonist Acyline Inhibits Basal and GnRH-Stimulated Serum Testosterone Concentrations in Male Dogs
title_sort A Single Administration of the GnRH Antagonist Acyline Inhibits Basal and GnRH-Stimulated Serum Testosterone Concentrations in Male Dogs
dc.creator.none.fl_str_mv Garcia Romero, Guadalupe
Mattioli, Guillermo Alberto
Rosa, Diana Esther
Díaz, Jorge Daniel
Abeyá, María Mercedes
Gobello, María Cristina
author Garcia Romero, Guadalupe
author_facet Garcia Romero, Guadalupe
Mattioli, Guillermo Alberto
Rosa, Diana Esther
Díaz, Jorge Daniel
Abeyá, María Mercedes
Gobello, María Cristina
author_role author
author2 Mattioli, Guillermo Alberto
Rosa, Diana Esther
Díaz, Jorge Daniel
Abeyá, María Mercedes
Gobello, María Cristina
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Gnrh
Acyline
Testoterone
Dog
topic Gnrh
Acyline
Testoterone
Dog
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv The objective of this study was to describe testosterone (T) response to GnRH challenge in antagonist-treated dogs over a 30-day period. Eight mongrel dogs were randomly assigned to either the GnRH antagonist acyline 330μg/kg sc (ACY; n=4) or a placebo group (PLA; n=4). The dogs were serially challenged with the GnRH agonist, buserelin 0.2μg/kg sc on days -1, 1, 3, 7, 10, 14, 21 and 30. On these days, blood samples for T determinations were collected before (-30min) and 60, 120 and 180min after the agonist injection. Basal (-30min) and post-GnRH agonist stimulation T values were compared by anova for repeated measures. Before treatments (day -1), there were no differences in basal T serum concentrations between groups (p>0.1). After treatments, basal T showed a significant interaction between treatment and day (p<0.05). Furthermore, when both groups were analysed independently, basal T varied in the ACY (p<0.01) but not in the PLA group (p>0.1). On day -1, before treatments, the stimulation tests had only a time effect (p=0.05) although on days 1 (p<0.01), 3 (p<0.01), 7 (p<0.01), 10 (p<0.01) and 14 (p<0.05), the response to the agonist differed between groups, becoming similar on days 21 (p>0.05) and 30 (p>0.05). It was concluded that, in dogs, a single administration of the GnRH antagonist prevented canine gonadal axis to physiologically respond to agonistic challenge during 14 days.
Fil: Garcia Romero, Guadalupe. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Ciencias Básicas. Laboratorio de Nutrición Mineral y Fisiología Reproductiva; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Mattioli, Guillermo Alberto. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Ciencias Básicas. Laboratorio de Nutrición Mineral y Fisiología Reproductiva; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Rosa, Diana Esther. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Ciencias Básicas. Laboratorio de Nutrición Mineral y Fisiología Reproductiva; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Díaz, Jorge Daniel. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Ciencias Básicas. Laboratorio de Nutrición Mineral y Fisiología Reproductiva; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Abeyá, María Mercedes. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Ciencias Básicas. Laboratorio de Nutrición Mineral y Fisiología Reproductiva; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gobello, María Cristina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Ciencias Básicas. Laboratorio de Nutrición Mineral y Fisiología Reproductiva; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description The objective of this study was to describe testosterone (T) response to GnRH challenge in antagonist-treated dogs over a 30-day period. Eight mongrel dogs were randomly assigned to either the GnRH antagonist acyline 330μg/kg sc (ACY; n=4) or a placebo group (PLA; n=4). The dogs were serially challenged with the GnRH agonist, buserelin 0.2μg/kg sc on days -1, 1, 3, 7, 10, 14, 21 and 30. On these days, blood samples for T determinations were collected before (-30min) and 60, 120 and 180min after the agonist injection. Basal (-30min) and post-GnRH agonist stimulation T values were compared by anova for repeated measures. Before treatments (day -1), there were no differences in basal T serum concentrations between groups (p>0.1). After treatments, basal T showed a significant interaction between treatment and day (p<0.05). Furthermore, when both groups were analysed independently, basal T varied in the ACY (p<0.01) but not in the PLA group (p>0.1). On day -1, before treatments, the stimulation tests had only a time effect (p=0.05) although on days 1 (p<0.01), 3 (p<0.01), 7 (p<0.01), 10 (p<0.01) and 14 (p<0.05), the response to the agonist differed between groups, becoming similar on days 21 (p>0.05) and 30 (p>0.05). It was concluded that, in dogs, a single administration of the GnRH antagonist prevented canine gonadal axis to physiologically respond to agonistic challenge during 14 days.
publishDate 2012
dc.date.none.fl_str_mv 2012-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/75355
Garcia Romero, Guadalupe; Mattioli, Guillermo Alberto; Rosa, Diana Esther; Díaz, Jorge Daniel; Abeyá, María Mercedes; et al.; A Single Administration of the GnRH Antagonist Acyline Inhibits Basal and GnRH-Stimulated Serum Testosterone Concentrations in Male Dogs; Wiley Blackwell Publishing, Inc; Reproduction in Domestic Animals; 47; 3; 6-2012
0936-6768
CONICET Digital
CONICET
url http://hdl.handle.net/11336/75355
identifier_str_mv Garcia Romero, Guadalupe; Mattioli, Guillermo Alberto; Rosa, Diana Esther; Díaz, Jorge Daniel; Abeyá, María Mercedes; et al.; A Single Administration of the GnRH Antagonist Acyline Inhibits Basal and GnRH-Stimulated Serum Testosterone Concentrations in Male Dogs; Wiley Blackwell Publishing, Inc; Reproduction in Domestic Animals; 47; 3; 6-2012
0936-6768
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1439-0531.2011.01898.x
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1439-0531.2011.01898.x
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.001348

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