Antimicotic effect of the essential oil of Aloysia triphylla against Candida species obtained from human pathologies

Autores
Oliva, Maria de Las Mercedes; Carezzano, Maria Evangelina; Gallucci, Mauro Nicolas; Demo, Mirta Susana
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The research of alternative substances to treat infections caused by Candida species is a need. Aromatic plants have the ability to produce secondary metabolites, such as essential oils (EO). The antimicrobial properties of Aloysia triphylla (L`Her.) Britton (cedrón) EO has been previously described. The aims of this work were to determine the antimicrobial activity and the effect on the cell structure of the EO of A. triphylla against Candida sp isolated from human illnesses. The EO was obtained by hydrodistilation of A. triphylla leaves. The minimum inhibitory concentration (MIC) was performed with microdilution method and the minimum fungicidal concentration (MFC) was determined. A. triphylla EO´s showed antifungal activity against all yeast: C.albicans, C.dubliniensis, C.glabrata, C.krusei, C.guillermondii, C.parapsilosis and C tropicalis which were resistant to fluconazol (150 mg/ml). The range of MIC values was from: 35 to 140 µg/ml and the MFC: 1842 to 2300µg/ml. The time of killing at the MFC against C. albicans (3 x 105 UFC/ml) was 140 min. The dates of OD620 and OD260 suggest lysis and loss of absorbing material, respectively. The HROM shows distortion in morphology and shape of the cell, with large vacuoles in the cytoplasm. These studies clearly show that A. triphylla EO is a promising alternative for the treatment of candidiasis.
Fil: Oliva, Maria de Las Mercedes. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Biotecnología Ambiental y Salud - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Biotecnología Ambiental y Salud; Argentina
Fil: Carezzano, Maria Evangelina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Biotecnología Ambiental y Salud - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Biotecnología Ambiental y Salud; Argentina
Fil: Gallucci, Mauro Nicolas. Universidad Nacional de Santiago del Estero. Instituto de Bionanotecnología del Noa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Bionanotecnología del Noa; Argentina
Fil: Demo, Mirta Susana. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina
Materia
Candidiasis
Aloysia triphylla
Essential oil
Antimicotic activity
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/190629
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/190629
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Antimicotic effect of the essential oil of Aloysia triphylla against Candida species obtained from human pathologiesOliva, Maria de Las MercedesCarezzano, Maria EvangelinaGallucci, Mauro NicolasDemo, Mirta SusanaCandidiasisAloysia triphyllaEssential oilAntimicotic activityhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The research of alternative substances to treat infections caused by Candida species is a need. Aromatic plants have the ability to produce secondary metabolites, such as essential oils (EO). The antimicrobial properties of Aloysia triphylla (L`Her.) Britton (cedrón) EO has been previously described. The aims of this work were to determine the antimicrobial activity and the effect on the cell structure of the EO of A. triphylla against Candida sp isolated from human illnesses. The EO was obtained by hydrodistilation of A. triphylla leaves. The minimum inhibitory concentration (MIC) was performed with microdilution method and the minimum fungicidal concentration (MFC) was determined. A. triphylla EO´s showed antifungal activity against all yeast: C.albicans, C.dubliniensis, C.glabrata, C.krusei, C.guillermondii, C.parapsilosis and C tropicalis which were resistant to fluconazol (150 mg/ml). The range of MIC values was from: 35 to 140 µg/ml and the MFC: 1842 to 2300µg/ml. The time of killing at the MFC against C. albicans (3 x 105 UFC/ml) was 140 min. The dates of OD620 and OD260 suggest lysis and loss of absorbing material, respectively. The HROM shows distortion in morphology and shape of the cell, with large vacuoles in the cytoplasm. These studies clearly show that A. triphylla EO is a promising alternative for the treatment of candidiasis.Fil: Oliva, Maria de Las Mercedes. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Biotecnología Ambiental y Salud - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Biotecnología Ambiental y Salud; ArgentinaFil: Carezzano, Maria Evangelina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Biotecnología Ambiental y Salud - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Biotecnología Ambiental y Salud; ArgentinaFil: Gallucci, Mauro Nicolas. Universidad Nacional de Santiago del Estero. Instituto de Bionanotecnología del Noa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Bionanotecnología del Noa; ArgentinaFil: Demo, Mirta Susana. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; ArgentinaNatural Products2011-07-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/190629Oliva, Maria de Las Mercedes; Carezzano, Maria Evangelina; Gallucci, Mauro Nicolas; Demo, Mirta Susana; Antimicotic effect of the essential oil of Aloysia triphylla against Candida species obtained from human pathologies; Natural Products; Natural Product Communications; 6; 7; 1-7-2011; 1039-10431934-578X1555-9475CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://journals.sagepub.com/doi/10.1177/1934578X1100600729info:eu-repo/semantics/altIdentifier/doi/10.1177/1934578X1100600729info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:29:54Zoai:ri.conicet.gov.ar:11336/190629instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:29:54.721CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Antimicotic effect of the essential oil of Aloysia triphylla against Candida species obtained from human pathologies
title Antimicotic effect of the essential oil of Aloysia triphylla against Candida species obtained from human pathologies
spellingShingle Antimicotic effect of the essential oil of Aloysia triphylla against Candida species obtained from human pathologies
Oliva, Maria de Las Mercedes
Candidiasis
Aloysia triphylla
Essential oil
Antimicotic activity
title_short Antimicotic effect of the essential oil of Aloysia triphylla against Candida species obtained from human pathologies
title_full Antimicotic effect of the essential oil of Aloysia triphylla against Candida species obtained from human pathologies
title_fullStr Antimicotic effect of the essential oil of Aloysia triphylla against Candida species obtained from human pathologies
title_full_unstemmed Antimicotic effect of the essential oil of Aloysia triphylla against Candida species obtained from human pathologies
title_sort Antimicotic effect of the essential oil of Aloysia triphylla against Candida species obtained from human pathologies
dc.creator.none.fl_str_mv Oliva, Maria de Las Mercedes
Carezzano, Maria Evangelina
Gallucci, Mauro Nicolas
Demo, Mirta Susana
author Oliva, Maria de Las Mercedes
author_facet Oliva, Maria de Las Mercedes
Carezzano, Maria Evangelina
Gallucci, Mauro Nicolas
Demo, Mirta Susana
author_role author
author2 Carezzano, Maria Evangelina
Gallucci, Mauro Nicolas
Demo, Mirta Susana
author2_role author
author
author
dc.subject.none.fl_str_mv Candidiasis
Aloysia triphylla
Essential oil
Antimicotic activity
topic Candidiasis
Aloysia triphylla
Essential oil
Antimicotic activity
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The research of alternative substances to treat infections caused by Candida species is a need. Aromatic plants have the ability to produce secondary metabolites, such as essential oils (EO). The antimicrobial properties of Aloysia triphylla (L`Her.) Britton (cedrón) EO has been previously described. The aims of this work were to determine the antimicrobial activity and the effect on the cell structure of the EO of A. triphylla against Candida sp isolated from human illnesses. The EO was obtained by hydrodistilation of A. triphylla leaves. The minimum inhibitory concentration (MIC) was performed with microdilution method and the minimum fungicidal concentration (MFC) was determined. A. triphylla EO´s showed antifungal activity against all yeast: C.albicans, C.dubliniensis, C.glabrata, C.krusei, C.guillermondii, C.parapsilosis and C tropicalis which were resistant to fluconazol (150 mg/ml). The range of MIC values was from: 35 to 140 µg/ml and the MFC: 1842 to 2300µg/ml. The time of killing at the MFC against C. albicans (3 x 105 UFC/ml) was 140 min. The dates of OD620 and OD260 suggest lysis and loss of absorbing material, respectively. The HROM shows distortion in morphology and shape of the cell, with large vacuoles in the cytoplasm. These studies clearly show that A. triphylla EO is a promising alternative for the treatment of candidiasis.
Fil: Oliva, Maria de Las Mercedes. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Biotecnología Ambiental y Salud - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Biotecnología Ambiental y Salud; Argentina
Fil: Carezzano, Maria Evangelina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Biotecnología Ambiental y Salud - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Biotecnología Ambiental y Salud; Argentina
Fil: Gallucci, Mauro Nicolas. Universidad Nacional de Santiago del Estero. Instituto de Bionanotecnología del Noa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Bionanotecnología del Noa; Argentina
Fil: Demo, Mirta Susana. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina
description The research of alternative substances to treat infections caused by Candida species is a need. Aromatic plants have the ability to produce secondary metabolites, such as essential oils (EO). The antimicrobial properties of Aloysia triphylla (L`Her.) Britton (cedrón) EO has been previously described. The aims of this work were to determine the antimicrobial activity and the effect on the cell structure of the EO of A. triphylla against Candida sp isolated from human illnesses. The EO was obtained by hydrodistilation of A. triphylla leaves. The minimum inhibitory concentration (MIC) was performed with microdilution method and the minimum fungicidal concentration (MFC) was determined. A. triphylla EO´s showed antifungal activity against all yeast: C.albicans, C.dubliniensis, C.glabrata, C.krusei, C.guillermondii, C.parapsilosis and C tropicalis which were resistant to fluconazol (150 mg/ml). The range of MIC values was from: 35 to 140 µg/ml and the MFC: 1842 to 2300µg/ml. The time of killing at the MFC against C. albicans (3 x 105 UFC/ml) was 140 min. The dates of OD620 and OD260 suggest lysis and loss of absorbing material, respectively. The HROM shows distortion in morphology and shape of the cell, with large vacuoles in the cytoplasm. These studies clearly show that A. triphylla EO is a promising alternative for the treatment of candidiasis.
publishDate 2011
dc.date.none.fl_str_mv 2011-07-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/190629
Oliva, Maria de Las Mercedes; Carezzano, Maria Evangelina; Gallucci, Mauro Nicolas; Demo, Mirta Susana; Antimicotic effect of the essential oil of Aloysia triphylla against Candida species obtained from human pathologies; Natural Products; Natural Product Communications; 6; 7; 1-7-2011; 1039-1043
1934-578X
1555-9475
CONICET Digital
CONICET
url http://hdl.handle.net/11336/190629
identifier_str_mv Oliva, Maria de Las Mercedes; Carezzano, Maria Evangelina; Gallucci, Mauro Nicolas; Demo, Mirta Susana; Antimicotic effect of the essential oil of Aloysia triphylla against Candida species obtained from human pathologies; Natural Products; Natural Product Communications; 6; 7; 1-7-2011; 1039-1043
1934-578X
1555-9475
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://journals.sagepub.com/doi/10.1177/1934578X1100600729
info:eu-repo/semantics/altIdentifier/doi/10.1177/1934578X1100600729
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Natural Products
publisher.none.fl_str_mv Natural Products
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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