Bacterial Biofilms Formed in Arsenic-containing Water: Bacterial Community Characterization

Autores
Rastelli, Silvia Elena; Rosales, Blanca Margarita; Viera, Marisa Rosana
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The present study approaches the analyses of structure and diversity of bacterial biofilms formed on different water distribution materials in the absence and in the presence of 5mg/L of As(V). Three commercial metals (cast iron, zinc and copper) and polypropylene were used as substrata for biofilm formation. Biofilms were observed using Scanning Electron Microscopy (SEM). Microbial ecology techniques (DNA amplification by the Polymerase Chain Reaction (PCR) and Denaturing Gradient Gel Electrophoresis (DGGE)) were applied to study the bacterial community attached to each substratum. The presence of arsenic-tolerant bacteria was investigated. The microscopic observations showed that biofilm development resulted higher on those coupons exposed to the arsenic-containing water. Besides, the presence of arsenic induced qualitative and quantitative changes in the planktonic bacterial community. The clustering analysis of the sessile community profiles showed that the nature of the substrata was a more important factor for the establishment of the community than the presence of arsenic in water. Culturable As-tolerant bacteria were obtained from most of the biofilms. The genetic diversity of the community adapted to the presence of the contaminant was less affected by the high concentration of As(V) in the culture, reflecting the presence of populations adapted to the contaminant.
Fil: Rastelli, Silvia Elena. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigaciones en Tecnología de Pinturas. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigaciones en Tecnología de Pinturas; Argentina
Fil: Rosales, Blanca Margarita. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigaciones en Tecnología de Pinturas. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigaciones en Tecnología de Pinturas; Argentina
Fil: Viera, Marisa Rosana. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigaciones en Tecnología de Pinturas. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigaciones en Tecnología de Pinturas; Argentina
Materia
Arsenic
Bacterial Communities
Biofilms
Dgge
Water
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/49297
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Bacterial Biofilms Formed in Arsenic-containing Water: Bacterial Community CharacterizationRastelli, Silvia ElenaRosales, Blanca MargaritaViera, Marisa RosanaArsenicBacterial CommunitiesBiofilmsDggeWaterhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The present study approaches the analyses of structure and diversity of bacterial biofilms formed on different water distribution materials in the absence and in the presence of 5mg/L of As(V). Three commercial metals (cast iron, zinc and copper) and polypropylene were used as substrata for biofilm formation. Biofilms were observed using Scanning Electron Microscopy (SEM). Microbial ecology techniques (DNA amplification by the Polymerase Chain Reaction (PCR) and Denaturing Gradient Gel Electrophoresis (DGGE)) were applied to study the bacterial community attached to each substratum. The presence of arsenic-tolerant bacteria was investigated. The microscopic observations showed that biofilm development resulted higher on those coupons exposed to the arsenic-containing water. Besides, the presence of arsenic induced qualitative and quantitative changes in the planktonic bacterial community. The clustering analysis of the sessile community profiles showed that the nature of the substrata was a more important factor for the establishment of the community than the presence of arsenic in water. Culturable As-tolerant bacteria were obtained from most of the biofilms. The genetic diversity of the community adapted to the presence of the contaminant was less affected by the high concentration of As(V) in the culture, reflecting the presence of populations adapted to the contaminant.Fil: Rastelli, Silvia Elena. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigaciones en Tecnología de Pinturas. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigaciones en Tecnología de Pinturas; ArgentinaFil: Rosales, Blanca Margarita. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigaciones en Tecnología de Pinturas. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigaciones en Tecnología de Pinturas; ArgentinaFil: Viera, Marisa Rosana. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigaciones en Tecnología de Pinturas. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigaciones en Tecnología de Pinturas; ArgentinaIWA Publishing2016-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/49297Rastelli, Silvia Elena; Rosales, Blanca Margarita; Viera, Marisa Rosana; Bacterial Biofilms Formed in Arsenic-containing Water: Bacterial Community Characterization; IWA Publishing; Journal Of Water Supply Research And Technology-aqua; 65; 1-2016; 1-110003-7214CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.2166/aqua.2015.137info:eu-repo/semantics/altIdentifier/url/http://aqua.iwaponline.com/content/65/1/1info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:57:14Zoai:ri.conicet.gov.ar:11336/49297instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:57:15.119CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Bacterial Biofilms Formed in Arsenic-containing Water: Bacterial Community Characterization
title Bacterial Biofilms Formed in Arsenic-containing Water: Bacterial Community Characterization
spellingShingle Bacterial Biofilms Formed in Arsenic-containing Water: Bacterial Community Characterization
Rastelli, Silvia Elena
Arsenic
Bacterial Communities
Biofilms
Dgge
Water
title_short Bacterial Biofilms Formed in Arsenic-containing Water: Bacterial Community Characterization
title_full Bacterial Biofilms Formed in Arsenic-containing Water: Bacterial Community Characterization
title_fullStr Bacterial Biofilms Formed in Arsenic-containing Water: Bacterial Community Characterization
title_full_unstemmed Bacterial Biofilms Formed in Arsenic-containing Water: Bacterial Community Characterization
title_sort Bacterial Biofilms Formed in Arsenic-containing Water: Bacterial Community Characterization
dc.creator.none.fl_str_mv Rastelli, Silvia Elena
Rosales, Blanca Margarita
Viera, Marisa Rosana
author Rastelli, Silvia Elena
author_facet Rastelli, Silvia Elena
Rosales, Blanca Margarita
Viera, Marisa Rosana
author_role author
author2 Rosales, Blanca Margarita
Viera, Marisa Rosana
author2_role author
author
dc.subject.none.fl_str_mv Arsenic
Bacterial Communities
Biofilms
Dgge
Water
topic Arsenic
Bacterial Communities
Biofilms
Dgge
Water
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The present study approaches the analyses of structure and diversity of bacterial biofilms formed on different water distribution materials in the absence and in the presence of 5mg/L of As(V). Three commercial metals (cast iron, zinc and copper) and polypropylene were used as substrata for biofilm formation. Biofilms were observed using Scanning Electron Microscopy (SEM). Microbial ecology techniques (DNA amplification by the Polymerase Chain Reaction (PCR) and Denaturing Gradient Gel Electrophoresis (DGGE)) were applied to study the bacterial community attached to each substratum. The presence of arsenic-tolerant bacteria was investigated. The microscopic observations showed that biofilm development resulted higher on those coupons exposed to the arsenic-containing water. Besides, the presence of arsenic induced qualitative and quantitative changes in the planktonic bacterial community. The clustering analysis of the sessile community profiles showed that the nature of the substrata was a more important factor for the establishment of the community than the presence of arsenic in water. Culturable As-tolerant bacteria were obtained from most of the biofilms. The genetic diversity of the community adapted to the presence of the contaminant was less affected by the high concentration of As(V) in the culture, reflecting the presence of populations adapted to the contaminant.
Fil: Rastelli, Silvia Elena. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigaciones en Tecnología de Pinturas. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigaciones en Tecnología de Pinturas; Argentina
Fil: Rosales, Blanca Margarita. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigaciones en Tecnología de Pinturas. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigaciones en Tecnología de Pinturas; Argentina
Fil: Viera, Marisa Rosana. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigaciones en Tecnología de Pinturas. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigaciones en Tecnología de Pinturas; Argentina
description The present study approaches the analyses of structure and diversity of bacterial biofilms formed on different water distribution materials in the absence and in the presence of 5mg/L of As(V). Three commercial metals (cast iron, zinc and copper) and polypropylene were used as substrata for biofilm formation. Biofilms were observed using Scanning Electron Microscopy (SEM). Microbial ecology techniques (DNA amplification by the Polymerase Chain Reaction (PCR) and Denaturing Gradient Gel Electrophoresis (DGGE)) were applied to study the bacterial community attached to each substratum. The presence of arsenic-tolerant bacteria was investigated. The microscopic observations showed that biofilm development resulted higher on those coupons exposed to the arsenic-containing water. Besides, the presence of arsenic induced qualitative and quantitative changes in the planktonic bacterial community. The clustering analysis of the sessile community profiles showed that the nature of the substrata was a more important factor for the establishment of the community than the presence of arsenic in water. Culturable As-tolerant bacteria were obtained from most of the biofilms. The genetic diversity of the community adapted to the presence of the contaminant was less affected by the high concentration of As(V) in the culture, reflecting the presence of populations adapted to the contaminant.
publishDate 2016
dc.date.none.fl_str_mv 2016-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/49297
Rastelli, Silvia Elena; Rosales, Blanca Margarita; Viera, Marisa Rosana; Bacterial Biofilms Formed in Arsenic-containing Water: Bacterial Community Characterization; IWA Publishing; Journal Of Water Supply Research And Technology-aqua; 65; 1-2016; 1-11
0003-7214
CONICET Digital
CONICET
url http://hdl.handle.net/11336/49297
identifier_str_mv Rastelli, Silvia Elena; Rosales, Blanca Margarita; Viera, Marisa Rosana; Bacterial Biofilms Formed in Arsenic-containing Water: Bacterial Community Characterization; IWA Publishing; Journal Of Water Supply Research And Technology-aqua; 65; 1-2016; 1-11
0003-7214
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.2166/aqua.2015.137
info:eu-repo/semantics/altIdentifier/url/http://aqua.iwaponline.com/content/65/1/1
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv IWA Publishing
publisher.none.fl_str_mv IWA Publishing
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269451327635456
score 13.13397

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