Effect of monocrotaline on blood brain barrier permeability in rats

Autores
Coll, Carlos; Fernandez, Ma. Alejandra; Coll, Sebastián; Coll, Tamara Anahí; Malliardi, Pablo; Perazzo, Juan Carlos; Filinger, Ester Julia; Lemberg, Abraham
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
We studied  if monocrotaline (MCT) portal hypertensive model modiies blood-brain barrier (BBB) condition. Male Wistar rats were used: Group MCT i.p. with MCT (60 mg/Kg of body weight) and Group Sham (GS) with saline. Forty-four days after injection rats were sacrified. Trypan blue and Evans blue tests were performed to evaluate BBB integrity in both groups. In cerebrospinal fluid (CF), protein and glucose were determined. Alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alcaline phsphatase were determined. Alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alcaline phosphatase were measured in serum samples. Portal pressure rose after MCT injection. Trypan blue diffused into hippocampus, Evans blue increased concentrations in brain of Group MCT and CT showed an increase in protein and glucose content in Group MCT. Serum AST, ALT and AP activities were significantly increase in Group MCT rats. It is suggested that liver damage and vasoconstrictor substances could produce portal hypertension, associated to toxic effects on brain and modifying thereby the BBB permeability
Fil: Coll, Carlos. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Ciencias Biológicas; Argentina
Fil: Fernandez, Ma. Alejandra. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Ciencias Biológicas; Argentina
Fil: Coll, Sebastián. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Ciencias Biológicas; Argentina
Fil: Coll, Tamara Anahí. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Malliardi, Pablo . Hospital Thompson; Argentina
Fil: Perazzo, Juan Carlos . Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Ciencias Biológicas; Argentina
Fil: Filinger, Ester Julia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Lemberg, Abraham . Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Ciencias Biológicas; Argentina
Materia
Blood-Brain Barrier
Liver Damage
Monocrotaline
Portal Hypertension
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/13193
Acceder
id CONICETDig_094f9b0fa4e3a8875f0b5d7f0c7eb730
oai_identifier_str oai:ri.conicet.gov.ar:11336/13193
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Effect of monocrotaline on blood brain barrier permeability in ratsColl, CarlosFernandez, Ma. AlejandraColl, SebastiánColl, Tamara AnahíMalliardi, Pablo Perazzo, Juan Carlos Filinger, Ester JuliaLemberg, Abraham Blood-Brain BarrierLiver DamageMonocrotalinePortal Hypertensionhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3We studied  if monocrotaline (MCT) portal hypertensive model modiies blood-brain barrier (BBB) condition. Male Wistar rats were used: Group MCT i.p. with MCT (60 mg/Kg of body weight) and Group Sham (GS) with saline. Forty-four days after injection rats were sacrified. Trypan blue and Evans blue tests were performed to evaluate BBB integrity in both groups. In cerebrospinal fluid (CF), protein and glucose were determined. Alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alcaline phsphatase were determined. Alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alcaline phosphatase were measured in serum samples. Portal pressure rose after MCT injection. Trypan blue diffused into hippocampus, Evans blue increased concentrations in brain of Group MCT and CT showed an increase in protein and glucose content in Group MCT. Serum AST, ALT and AP activities were significantly increase in Group MCT rats. It is suggested that liver damage and vasoconstrictor substances could produce portal hypertension, associated to toxic effects on brain and modifying thereby the BBB permeabilityFil: Coll, Carlos. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Ciencias Biológicas; ArgentinaFil: Fernandez, Ma. Alejandra. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Ciencias Biológicas; ArgentinaFil: Coll, Sebastián. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Ciencias Biológicas; ArgentinaFil: Coll, Tamara Anahí. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaFil: Malliardi, Pablo . Hospital Thompson; ArgentinaFil: Perazzo, Juan Carlos . Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Ciencias Biológicas; ArgentinaFil: Filinger, Ester Julia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaFil: Lemberg, Abraham . Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Ciencias Biológicas; ArgentinaColegio Farmaceuticos Provincia de Buenos Aires2011-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/13193Coll, Carlos; Fernandez, Ma. Alejandra; Coll, Sebastián; Coll, Tamara Anahí; Malliardi, Pablo ; et al.; Effect of monocrotaline on blood brain barrier permeability in rats; Colegio Farmaceuticos Provincia de Buenos Aires; Latin American Journal of Pharmacy; 30; 2; 2-2011; 412-4160326-2383enginfo:eu-repo/semantics/altIdentifier/url/http://www.latamjpharm.org/previous_issue.php?vol=30&num=2info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-01-14T11:58:49Zoai:ri.conicet.gov.ar:11336/13193instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-01-14 11:58:50.14CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effect of monocrotaline on blood brain barrier permeability in rats
title Effect of monocrotaline on blood brain barrier permeability in rats
spellingShingle Effect of monocrotaline on blood brain barrier permeability in rats
Coll, Carlos
Blood-Brain Barrier
Liver Damage
Monocrotaline
Portal Hypertension
title_short Effect of monocrotaline on blood brain barrier permeability in rats
title_full Effect of monocrotaline on blood brain barrier permeability in rats
title_fullStr Effect of monocrotaline on blood brain barrier permeability in rats
title_full_unstemmed Effect of monocrotaline on blood brain barrier permeability in rats
title_sort Effect of monocrotaline on blood brain barrier permeability in rats
dc.creator.none.fl_str_mv Coll, Carlos
Fernandez, Ma. Alejandra
Coll, Sebastián
Coll, Tamara Anahí
Malliardi, Pablo
Perazzo, Juan Carlos
Filinger, Ester Julia
Lemberg, Abraham
author Coll, Carlos
author_facet Coll, Carlos
Fernandez, Ma. Alejandra
Coll, Sebastián
Coll, Tamara Anahí
Malliardi, Pablo
Perazzo, Juan Carlos
Filinger, Ester Julia
Lemberg, Abraham
author_role author
author2 Fernandez, Ma. Alejandra
Coll, Sebastián
Coll, Tamara Anahí
Malliardi, Pablo
Perazzo, Juan Carlos
Filinger, Ester Julia
Lemberg, Abraham
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Blood-Brain Barrier
Liver Damage
Monocrotaline
Portal Hypertension
topic Blood-Brain Barrier
Liver Damage
Monocrotaline
Portal Hypertension
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv We studied  if monocrotaline (MCT) portal hypertensive model modiies blood-brain barrier (BBB) condition. Male Wistar rats were used: Group MCT i.p. with MCT (60 mg/Kg of body weight) and Group Sham (GS) with saline. Forty-four days after injection rats were sacrified. Trypan blue and Evans blue tests were performed to evaluate BBB integrity in both groups. In cerebrospinal fluid (CF), protein and glucose were determined. Alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alcaline phsphatase were determined. Alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alcaline phosphatase were measured in serum samples. Portal pressure rose after MCT injection. Trypan blue diffused into hippocampus, Evans blue increased concentrations in brain of Group MCT and CT showed an increase in protein and glucose content in Group MCT. Serum AST, ALT and AP activities were significantly increase in Group MCT rats. It is suggested that liver damage and vasoconstrictor substances could produce portal hypertension, associated to toxic effects on brain and modifying thereby the BBB permeability
Fil: Coll, Carlos. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Ciencias Biológicas; Argentina
Fil: Fernandez, Ma. Alejandra. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Ciencias Biológicas; Argentina
Fil: Coll, Sebastián. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Ciencias Biológicas; Argentina
Fil: Coll, Tamara Anahí. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Malliardi, Pablo . Hospital Thompson; Argentina
Fil: Perazzo, Juan Carlos . Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Ciencias Biológicas; Argentina
Fil: Filinger, Ester Julia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Lemberg, Abraham . Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Ciencias Biológicas; Argentina
description We studied  if monocrotaline (MCT) portal hypertensive model modiies blood-brain barrier (BBB) condition. Male Wistar rats were used: Group MCT i.p. with MCT (60 mg/Kg of body weight) and Group Sham (GS) with saline. Forty-four days after injection rats were sacrified. Trypan blue and Evans blue tests were performed to evaluate BBB integrity in both groups. In cerebrospinal fluid (CF), protein and glucose were determined. Alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alcaline phsphatase were determined. Alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alcaline phosphatase were measured in serum samples. Portal pressure rose after MCT injection. Trypan blue diffused into hippocampus, Evans blue increased concentrations in brain of Group MCT and CT showed an increase in protein and glucose content in Group MCT. Serum AST, ALT and AP activities were significantly increase in Group MCT rats. It is suggested that liver damage and vasoconstrictor substances could produce portal hypertension, associated to toxic effects on brain and modifying thereby the BBB permeability
publishDate 2011
dc.date.none.fl_str_mv 2011-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/13193
Coll, Carlos; Fernandez, Ma. Alejandra; Coll, Sebastián; Coll, Tamara Anahí; Malliardi, Pablo ; et al.; Effect of monocrotaline on blood brain barrier permeability in rats; Colegio Farmaceuticos Provincia de Buenos Aires; Latin American Journal of Pharmacy; 30; 2; 2-2011; 412-416
0326-2383
url http://hdl.handle.net/11336/13193
identifier_str_mv Coll, Carlos; Fernandez, Ma. Alejandra; Coll, Sebastián; Coll, Tamara Anahí; Malliardi, Pablo ; et al.; Effect of monocrotaline on blood brain barrier permeability in rats; Colegio Farmaceuticos Provincia de Buenos Aires; Latin American Journal of Pharmacy; 30; 2; 2-2011; 412-416
0326-2383
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.latamjpharm.org/previous_issue.php?vol=30&num=2
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Colegio Farmaceuticos Provincia de Buenos Aires
publisher.none.fl_str_mv Colegio Farmaceuticos Provincia de Buenos Aires
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1854321218589556736
score 13.071171

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