Efficacy of oxyclozanide against adult Paramphistomum leydeni in naturally infected sheep

Autores
Sanabria, Rodrigo Eduardo Fabrizio; Moreno, Laura Susana; Alvarez, Luis Ignacio; Lanusse, Carlos Edmundo; Romero, Jorge
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The aim of the current study was to assess oxyclozanide (OCZ) efficacy against Paramphistomum leydeni in naturally infected adult sheep. OCZ concentrations in blood stream and gastrointestinal fluids collected from treated animals were also measured. Fifteen P. leydeni naturally infected sheep were randomly divided into two groups: untreated control (n = 5) and treated (n = 10). The treated group was orally drenched with OCZ (20 mg/kg, day 0). A second dose was administered 72 h later. Faecal samples were taken at days 0, +3 and +5. Five sheep from both groups were slaughtered at day +5. At necropsies, rumen, abomasum and small intestine were examined for adult and immature flukes. All recovered flukes were counted and the treatment efficacy was estimated. Additionally, serum and gastrointestinal fluid content (ruminal, abomasal and small intestine) samples, obtained from five treated animals at day +5, were analyzed by HPLC to measure OCZ concentrations. OCZ showed high efficacy (99%) against mature P. leydeni. The post-treatment egg reduction was also high after the first dose with values ranging from 98.4% (day +3) to 99.5% (day +5). The highest OCZ concentrations were measured in serum (20.7 ± 11.5 μg/mL) followed by the small intestinal fluid (6.00 ± 4.50 μg/mL). Very low OCZ concentrations (ranging between 0.05 and 0.02 μg/mL) were measured in ruminal and abomasal fluids. OCZ administered to sheep twice (20 mg/kg) by the oral route was highly efficacious against mature stages of P. leydeni in naturally infected sheep. Despite a high drug concentration at the intestinal fluid, OCZ efficacy against immature stages could not be assessed. OCZ efficacy and assessment of its concentration profiles in different tissues are considered a contribution to the scarce information available on this ruminant fluke.
Fil: Sanabria, Rodrigo Eduardo Fabrizio. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Clinica. Centro de Diagnostico e Investigaciones Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Moreno, Laura Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Alvarez, Luis Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Lanusse, Carlos Edmundo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Romero, Jorge. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Clinica. Centro de Diagnostico e Investigaciones Veterinarias; Argentina
Materia
Paramphistomum Leydeni
Oxyclozanide
Efficacy
Sheep
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/33284
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Efficacy of oxyclozanide against adult Paramphistomum leydeni in naturally infected sheepSanabria, Rodrigo Eduardo FabrizioMoreno, Laura SusanaAlvarez, Luis IgnacioLanusse, Carlos EdmundoRomero, JorgeParamphistomum LeydeniOxyclozanideEfficacySheephttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4The aim of the current study was to assess oxyclozanide (OCZ) efficacy against Paramphistomum leydeni in naturally infected adult sheep. OCZ concentrations in blood stream and gastrointestinal fluids collected from treated animals were also measured. Fifteen P. leydeni naturally infected sheep were randomly divided into two groups: untreated control (n = 5) and treated (n = 10). The treated group was orally drenched with OCZ (20 mg/kg, day 0). A second dose was administered 72 h later. Faecal samples were taken at days 0, +3 and +5. Five sheep from both groups were slaughtered at day +5. At necropsies, rumen, abomasum and small intestine were examined for adult and immature flukes. All recovered flukes were counted and the treatment efficacy was estimated. Additionally, serum and gastrointestinal fluid content (ruminal, abomasal and small intestine) samples, obtained from five treated animals at day +5, were analyzed by HPLC to measure OCZ concentrations. OCZ showed high efficacy (99%) against mature P. leydeni. The post-treatment egg reduction was also high after the first dose with values ranging from 98.4% (day +3) to 99.5% (day +5). The highest OCZ concentrations were measured in serum (20.7 ± 11.5 μg/mL) followed by the small intestinal fluid (6.00 ± 4.50 μg/mL). Very low OCZ concentrations (ranging between 0.05 and 0.02 μg/mL) were measured in ruminal and abomasal fluids. OCZ administered to sheep twice (20 mg/kg) by the oral route was highly efficacious against mature stages of P. leydeni in naturally infected sheep. Despite a high drug concentration at the intestinal fluid, OCZ efficacy against immature stages could not be assessed. OCZ efficacy and assessment of its concentration profiles in different tissues are considered a contribution to the scarce information available on this ruminant fluke.Fil: Sanabria, Rodrigo Eduardo Fabrizio. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Clinica. Centro de Diagnostico e Investigaciones Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Moreno, Laura Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; ArgentinaFil: Alvarez, Luis Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; ArgentinaFil: Lanusse, Carlos Edmundo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; ArgentinaFil: Romero, Jorge. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Clinica. Centro de Diagnostico e Investigaciones Veterinarias; ArgentinaElsevier Science2014-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/33284Moreno, Laura Susana; Lanusse, Carlos Edmundo; Romero, Jorge; Sanabria, Rodrigo Eduardo Fabrizio; Alvarez, Luis Ignacio; Efficacy of oxyclozanide against adult Paramphistomum leydeni in naturally infected sheep; Elsevier Science; Veterinary Parasitology; 206; 3-4; 10-2014; 277-2810304-4017CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.vetpar.2014.09.022info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S030440171400507Xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:02:14Zoai:ri.conicet.gov.ar:11336/33284instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:02:15.091CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Efficacy of oxyclozanide against adult Paramphistomum leydeni in naturally infected sheep
title Efficacy of oxyclozanide against adult Paramphistomum leydeni in naturally infected sheep
spellingShingle Efficacy of oxyclozanide against adult Paramphistomum leydeni in naturally infected sheep
Sanabria, Rodrigo Eduardo Fabrizio
Paramphistomum Leydeni
Oxyclozanide
Efficacy
Sheep
title_short Efficacy of oxyclozanide against adult Paramphistomum leydeni in naturally infected sheep
title_full Efficacy of oxyclozanide against adult Paramphistomum leydeni in naturally infected sheep
title_fullStr Efficacy of oxyclozanide against adult Paramphistomum leydeni in naturally infected sheep
title_full_unstemmed Efficacy of oxyclozanide against adult Paramphistomum leydeni in naturally infected sheep
title_sort Efficacy of oxyclozanide against adult Paramphistomum leydeni in naturally infected sheep
dc.creator.none.fl_str_mv Sanabria, Rodrigo Eduardo Fabrizio
Moreno, Laura Susana
Alvarez, Luis Ignacio
Lanusse, Carlos Edmundo
Romero, Jorge
author Sanabria, Rodrigo Eduardo Fabrizio
author_facet Sanabria, Rodrigo Eduardo Fabrizio
Moreno, Laura Susana
Alvarez, Luis Ignacio
Lanusse, Carlos Edmundo
Romero, Jorge
author_role author
author2 Moreno, Laura Susana
Alvarez, Luis Ignacio
Lanusse, Carlos Edmundo
Romero, Jorge
author2_role author
author
author
author
dc.subject.none.fl_str_mv Paramphistomum Leydeni
Oxyclozanide
Efficacy
Sheep
topic Paramphistomum Leydeni
Oxyclozanide
Efficacy
Sheep
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv The aim of the current study was to assess oxyclozanide (OCZ) efficacy against Paramphistomum leydeni in naturally infected adult sheep. OCZ concentrations in blood stream and gastrointestinal fluids collected from treated animals were also measured. Fifteen P. leydeni naturally infected sheep were randomly divided into two groups: untreated control (n = 5) and treated (n = 10). The treated group was orally drenched with OCZ (20 mg/kg, day 0). A second dose was administered 72 h later. Faecal samples were taken at days 0, +3 and +5. Five sheep from both groups were slaughtered at day +5. At necropsies, rumen, abomasum and small intestine were examined for adult and immature flukes. All recovered flukes were counted and the treatment efficacy was estimated. Additionally, serum and gastrointestinal fluid content (ruminal, abomasal and small intestine) samples, obtained from five treated animals at day +5, were analyzed by HPLC to measure OCZ concentrations. OCZ showed high efficacy (99%) against mature P. leydeni. The post-treatment egg reduction was also high after the first dose with values ranging from 98.4% (day +3) to 99.5% (day +5). The highest OCZ concentrations were measured in serum (20.7 ± 11.5 μg/mL) followed by the small intestinal fluid (6.00 ± 4.50 μg/mL). Very low OCZ concentrations (ranging between 0.05 and 0.02 μg/mL) were measured in ruminal and abomasal fluids. OCZ administered to sheep twice (20 mg/kg) by the oral route was highly efficacious against mature stages of P. leydeni in naturally infected sheep. Despite a high drug concentration at the intestinal fluid, OCZ efficacy against immature stages could not be assessed. OCZ efficacy and assessment of its concentration profiles in different tissues are considered a contribution to the scarce information available on this ruminant fluke.
Fil: Sanabria, Rodrigo Eduardo Fabrizio. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Clinica. Centro de Diagnostico e Investigaciones Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Moreno, Laura Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Alvarez, Luis Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Lanusse, Carlos Edmundo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Romero, Jorge. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Clinica. Centro de Diagnostico e Investigaciones Veterinarias; Argentina
description The aim of the current study was to assess oxyclozanide (OCZ) efficacy against Paramphistomum leydeni in naturally infected adult sheep. OCZ concentrations in blood stream and gastrointestinal fluids collected from treated animals were also measured. Fifteen P. leydeni naturally infected sheep were randomly divided into two groups: untreated control (n = 5) and treated (n = 10). The treated group was orally drenched with OCZ (20 mg/kg, day 0). A second dose was administered 72 h later. Faecal samples were taken at days 0, +3 and +5. Five sheep from both groups were slaughtered at day +5. At necropsies, rumen, abomasum and small intestine were examined for adult and immature flukes. All recovered flukes were counted and the treatment efficacy was estimated. Additionally, serum and gastrointestinal fluid content (ruminal, abomasal and small intestine) samples, obtained from five treated animals at day +5, were analyzed by HPLC to measure OCZ concentrations. OCZ showed high efficacy (99%) against mature P. leydeni. The post-treatment egg reduction was also high after the first dose with values ranging from 98.4% (day +3) to 99.5% (day +5). The highest OCZ concentrations were measured in serum (20.7 ± 11.5 μg/mL) followed by the small intestinal fluid (6.00 ± 4.50 μg/mL). Very low OCZ concentrations (ranging between 0.05 and 0.02 μg/mL) were measured in ruminal and abomasal fluids. OCZ administered to sheep twice (20 mg/kg) by the oral route was highly efficacious against mature stages of P. leydeni in naturally infected sheep. Despite a high drug concentration at the intestinal fluid, OCZ efficacy against immature stages could not be assessed. OCZ efficacy and assessment of its concentration profiles in different tissues are considered a contribution to the scarce information available on this ruminant fluke.
publishDate 2014
dc.date.none.fl_str_mv 2014-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/33284
Moreno, Laura Susana; Lanusse, Carlos Edmundo; Romero, Jorge; Sanabria, Rodrigo Eduardo Fabrizio; Alvarez, Luis Ignacio; Efficacy of oxyclozanide against adult Paramphistomum leydeni in naturally infected sheep; Elsevier Science; Veterinary Parasitology; 206; 3-4; 10-2014; 277-281
0304-4017
CONICET Digital
CONICET
url http://hdl.handle.net/11336/33284
identifier_str_mv Moreno, Laura Susana; Lanusse, Carlos Edmundo; Romero, Jorge; Sanabria, Rodrigo Eduardo Fabrizio; Alvarez, Luis Ignacio; Efficacy of oxyclozanide against adult Paramphistomum leydeni in naturally infected sheep; Elsevier Science; Veterinary Parasitology; 206; 3-4; 10-2014; 277-281
0304-4017
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.vetpar.2014.09.022
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S030440171400507X
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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