The effects of Phycocyanobilin on experimental arthritis involve the reduction in nociception and synovial neutrophil infiltration, inhibition of cytokine production, and modulatio...
- Autores
- Marín Prida, Javier; Rodríguez Ulloa, Arielis; Besada, Vladimir; Llopiz Arzuaga, Alexey; Batista, Nathália Vieira; Hernández González, Ignacio; Pavón Fuentes, Nancy; Marciano Vieira, Érica Leandro; Falcón Cama, Viviana; Acosta, Emilio F.; Martínez Donato, Gillian; Cervantes Llanos, Majel; Lingfeng, Dai; González, Luis J.; Fernández Massó, Julio Raúl; Guillén Nieto, Gerardo; Pentón Arias, Eduardo; Amaral, Flávio Almeida; Teixeira, Mauro Martins; Pentón Rol, Giselle
- Año de publicación
- 2023
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Introduction: The antinociceptive and pharmacological activities of C-Phycocyanin (C-PC) and Phycocyanobilin (PCB) in the context of inflammatory arthritis remain unexplored so far. In the present study, we aimed to assess the protective actions of these compounds in an experimental mice model that replicates key aspects of human rheumatoid arthritis.Methods: Antigen-induced arthritis (AIA) was established by intradermal injection of methylated bovine serum albumin in C57BL/6 mice, and one hour before the antigen challenge, either C-PC (2, 4, or 8 mg/kg) or PCB (0.1 or 1 mg/kg) were administered intraperitoneally. Proteome profiling was also conducted on glutamate-exposed SH-SY5Y neuronal cells to evaluate the PCB impact on this key signaling pathway associated with nociceptive neuronal sensitization.Results and discussion: C-PC and PCB notably ameliorated hypernociception, synovial neutrophil infiltration, myeloperoxidase activity, and the periarticular cytokine concentration of IFN-γ, TNF-α, IL-17A, and IL-4 dose-dependently in AIA mice. In addition, 1 mg/kg PCB downregulated the gene expression for T-bet, RORγ, and IFN-γ in the popliteal lymph nodes, accompanied by a significant reduction in the pathological arthritic index of AIA mice. Noteworthy, neuronal proteome analysis revealed that PCB modulated biological processes such as pain, inflammation, and glutamatergic transmission, all of which are involved in arthritic pathology.Conclusions: These findings demonstrate the remarkable efficacy of PCB in alleviating the nociception and inflammation in the AIA mice model and shed new light on mechanisms underlying the PCB modulation of the neuronal proteome. This research work opens a new avenue to explore the translational potential of PCB in developing a therapeutic strategy for inflammation and pain in rheumatoid arthritis.
Fil: Marín Prida, Javier. University of Havana; Cuba
Fil: Rodríguez Ulloa, Arielis. Center for Genetic Engineering and Biotechnology; Cuba
Fil: Besada, Vladimir. Center for Genetic Engineering and Biotechnology; Cuba
Fil: Llopiz Arzuaga, Alexey. Center for Genetic Engineering and Biotechnology; Cuba
Fil: Batista, Nathália Vieira. Universidade Federal de Minas Gerais; Brasil
Fil: Hernández González, Ignacio. No especifíca;
Fil: Pavón Fuentes, Nancy. No especifíca;
Fil: Marciano Vieira, Érica Leandro. Universidade Federal de Minas Gerais; Brasil
Fil: Falcón Cama, Viviana. Center for Genetic Engineering and Biotechnology; Cuba
Fil: Acosta, Emilio F.. Center for Genetic Engineering and Biotechnology; Cuba
Fil: Martínez Donato, Gillian. Center for Genetic Engineering and Biotechnology; Cuba
Fil: Cervantes Llanos, Majel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Lingfeng, Dai. No especifíca;
Fil: González, Luis J.. Center for Genetic Engineering and Biotechnology; Cuba
Fil: Fernández Massó, Julio Raúl. Center for Genetic Engineering and Biotechnology; Cuba
Fil: Guillén Nieto, Gerardo. Center for Genetic Engineering and Biotechnology; Cuba
Fil: Pentón Arias, Eduardo. Center for Genetic Engineering and Biotechnology; Cuba
Fil: Amaral, Flávio Almeida. Universidade Federal de Minas Gerais; Brasil
Fil: Teixeira, Mauro Martins. Universidade Federal de Minas Gerais; Brasil
Fil: Pentón Rol, Giselle. Center for Genetic Engineering and Biotechnology; Cuba - Materia
-
experimental arthritis
neuronal proteome
PHYCOCYANOBILIN
INFLAMMATION - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
.jpg)
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/256522
Ver los metadatos del registro completo
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The effects of Phycocyanobilin on experimental arthritis involve the reduction in nociception and synovial neutrophil infiltration, inhibition of cytokine production, and modulation of the neuronal proteomeMarín Prida, JavierRodríguez Ulloa, ArielisBesada, VladimirLlopiz Arzuaga, AlexeyBatista, Nathália VieiraHernández González, IgnacioPavón Fuentes, NancyMarciano Vieira, Érica LeandroFalcón Cama, VivianaAcosta, Emilio F.Martínez Donato, GillianCervantes Llanos, MajelLingfeng, DaiGonzález, Luis J.Fernández Massó, Julio RaúlGuillén Nieto, GerardoPentón Arias, EduardoAmaral, Flávio AlmeidaTeixeira, Mauro MartinsPentón Rol, Giselleexperimental arthritisneuronal proteomePHYCOCYANOBILININFLAMMATIONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Introduction: The antinociceptive and pharmacological activities of C-Phycocyanin (C-PC) and Phycocyanobilin (PCB) in the context of inflammatory arthritis remain unexplored so far. In the present study, we aimed to assess the protective actions of these compounds in an experimental mice model that replicates key aspects of human rheumatoid arthritis.Methods: Antigen-induced arthritis (AIA) was established by intradermal injection of methylated bovine serum albumin in C57BL/6 mice, and one hour before the antigen challenge, either C-PC (2, 4, or 8 mg/kg) or PCB (0.1 or 1 mg/kg) were administered intraperitoneally. Proteome profiling was also conducted on glutamate-exposed SH-SY5Y neuronal cells to evaluate the PCB impact on this key signaling pathway associated with nociceptive neuronal sensitization.Results and discussion: C-PC and PCB notably ameliorated hypernociception, synovial neutrophil infiltration, myeloperoxidase activity, and the periarticular cytokine concentration of IFN-γ, TNF-α, IL-17A, and IL-4 dose-dependently in AIA mice. In addition, 1 mg/kg PCB downregulated the gene expression for T-bet, RORγ, and IFN-γ in the popliteal lymph nodes, accompanied by a significant reduction in the pathological arthritic index of AIA mice. Noteworthy, neuronal proteome analysis revealed that PCB modulated biological processes such as pain, inflammation, and glutamatergic transmission, all of which are involved in arthritic pathology.Conclusions: These findings demonstrate the remarkable efficacy of PCB in alleviating the nociception and inflammation in the AIA mice model and shed new light on mechanisms underlying the PCB modulation of the neuronal proteome. This research work opens a new avenue to explore the translational potential of PCB in developing a therapeutic strategy for inflammation and pain in rheumatoid arthritis.Fil: Marín Prida, Javier. University of Havana; CubaFil: Rodríguez Ulloa, Arielis. Center for Genetic Engineering and Biotechnology; CubaFil: Besada, Vladimir. Center for Genetic Engineering and Biotechnology; CubaFil: Llopiz Arzuaga, Alexey. Center for Genetic Engineering and Biotechnology; CubaFil: Batista, Nathália Vieira. Universidade Federal de Minas Gerais; BrasilFil: Hernández González, Ignacio. No especifíca;Fil: Pavón Fuentes, Nancy. No especifíca;Fil: Marciano Vieira, Érica Leandro. Universidade Federal de Minas Gerais; BrasilFil: Falcón Cama, Viviana. Center for Genetic Engineering and Biotechnology; CubaFil: Acosta, Emilio F.. Center for Genetic Engineering and Biotechnology; CubaFil: Martínez Donato, Gillian. Center for Genetic Engineering and Biotechnology; CubaFil: Cervantes Llanos, Majel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; ArgentinaFil: Lingfeng, Dai. No especifíca;Fil: González, Luis J.. Center for Genetic Engineering and Biotechnology; CubaFil: Fernández Massó, Julio Raúl. Center for Genetic Engineering and Biotechnology; CubaFil: Guillén Nieto, Gerardo. Center for Genetic Engineering and Biotechnology; CubaFil: Pentón Arias, Eduardo. Center for Genetic Engineering and Biotechnology; CubaFil: Amaral, Flávio Almeida. Universidade Federal de Minas Gerais; BrasilFil: Teixeira, Mauro Martins. Universidade Federal de Minas Gerais; BrasilFil: Pentón Rol, Giselle. Center for Genetic Engineering and Biotechnology; CubaFrontiers Media2023-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/256522Marín Prida, Javier; Rodríguez Ulloa, Arielis; Besada, Vladimir; Llopiz Arzuaga, Alexey; Batista, Nathália Vieira; et al.; The effects of Phycocyanobilin on experimental arthritis involve the reduction in nociception and synovial neutrophil infiltration, inhibition of cytokine production, and modulation of the neuronal proteome; Frontiers Media; Frontiers in Immunology; 14; 10-2023; 1-181664-3224CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fimmu.2023.1227268/fullinfo:eu-repo/semantics/altIdentifier/doi/10.3389/fimmu.2023.1227268info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:05:35Zoai:ri.conicet.gov.ar:11336/256522instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:05:35.886CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
| dc.title.none.fl_str_mv |
The effects of Phycocyanobilin on experimental arthritis involve the reduction in nociception and synovial neutrophil infiltration, inhibition of cytokine production, and modulation of the neuronal proteome |
| title |
The effects of Phycocyanobilin on experimental arthritis involve the reduction in nociception and synovial neutrophil infiltration, inhibition of cytokine production, and modulation of the neuronal proteome |
| spellingShingle |
The effects of Phycocyanobilin on experimental arthritis involve the reduction in nociception and synovial neutrophil infiltration, inhibition of cytokine production, and modulation of the neuronal proteome Marín Prida, Javier experimental arthritis neuronal proteome PHYCOCYANOBILIN INFLAMMATION |
| title_short |
The effects of Phycocyanobilin on experimental arthritis involve the reduction in nociception and synovial neutrophil infiltration, inhibition of cytokine production, and modulation of the neuronal proteome |
| title_full |
The effects of Phycocyanobilin on experimental arthritis involve the reduction in nociception and synovial neutrophil infiltration, inhibition of cytokine production, and modulation of the neuronal proteome |
| title_fullStr |
The effects of Phycocyanobilin on experimental arthritis involve the reduction in nociception and synovial neutrophil infiltration, inhibition of cytokine production, and modulation of the neuronal proteome |
| title_full_unstemmed |
The effects of Phycocyanobilin on experimental arthritis involve the reduction in nociception and synovial neutrophil infiltration, inhibition of cytokine production, and modulation of the neuronal proteome |
| title_sort |
The effects of Phycocyanobilin on experimental arthritis involve the reduction in nociception and synovial neutrophil infiltration, inhibition of cytokine production, and modulation of the neuronal proteome |
| dc.creator.none.fl_str_mv |
Marín Prida, Javier Rodríguez Ulloa, Arielis Besada, Vladimir Llopiz Arzuaga, Alexey Batista, Nathália Vieira Hernández González, Ignacio Pavón Fuentes, Nancy Marciano Vieira, Érica Leandro Falcón Cama, Viviana Acosta, Emilio F. Martínez Donato, Gillian Cervantes Llanos, Majel Lingfeng, Dai González, Luis J. Fernández Massó, Julio Raúl Guillén Nieto, Gerardo Pentón Arias, Eduardo Amaral, Flávio Almeida Teixeira, Mauro Martins Pentón Rol, Giselle |
| author |
Marín Prida, Javier |
| author_facet |
Marín Prida, Javier Rodríguez Ulloa, Arielis Besada, Vladimir Llopiz Arzuaga, Alexey Batista, Nathália Vieira Hernández González, Ignacio Pavón Fuentes, Nancy Marciano Vieira, Érica Leandro Falcón Cama, Viviana Acosta, Emilio F. Martínez Donato, Gillian Cervantes Llanos, Majel Lingfeng, Dai González, Luis J. Fernández Massó, Julio Raúl Guillén Nieto, Gerardo Pentón Arias, Eduardo Amaral, Flávio Almeida Teixeira, Mauro Martins Pentón Rol, Giselle |
| author_role |
author |
| author2 |
Rodríguez Ulloa, Arielis Besada, Vladimir Llopiz Arzuaga, Alexey Batista, Nathália Vieira Hernández González, Ignacio Pavón Fuentes, Nancy Marciano Vieira, Érica Leandro Falcón Cama, Viviana Acosta, Emilio F. Martínez Donato, Gillian Cervantes Llanos, Majel Lingfeng, Dai González, Luis J. Fernández Massó, Julio Raúl Guillén Nieto, Gerardo Pentón Arias, Eduardo Amaral, Flávio Almeida Teixeira, Mauro Martins Pentón Rol, Giselle |
| author2_role |
author author author author author author author author author author author author author author author author author author author |
| dc.subject.none.fl_str_mv |
experimental arthritis neuronal proteome PHYCOCYANOBILIN INFLAMMATION |
| topic |
experimental arthritis neuronal proteome PHYCOCYANOBILIN INFLAMMATION |
| purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
| dc.description.none.fl_txt_mv |
Introduction: The antinociceptive and pharmacological activities of C-Phycocyanin (C-PC) and Phycocyanobilin (PCB) in the context of inflammatory arthritis remain unexplored so far. In the present study, we aimed to assess the protective actions of these compounds in an experimental mice model that replicates key aspects of human rheumatoid arthritis.Methods: Antigen-induced arthritis (AIA) was established by intradermal injection of methylated bovine serum albumin in C57BL/6 mice, and one hour before the antigen challenge, either C-PC (2, 4, or 8 mg/kg) or PCB (0.1 or 1 mg/kg) were administered intraperitoneally. Proteome profiling was also conducted on glutamate-exposed SH-SY5Y neuronal cells to evaluate the PCB impact on this key signaling pathway associated with nociceptive neuronal sensitization.Results and discussion: C-PC and PCB notably ameliorated hypernociception, synovial neutrophil infiltration, myeloperoxidase activity, and the periarticular cytokine concentration of IFN-γ, TNF-α, IL-17A, and IL-4 dose-dependently in AIA mice. In addition, 1 mg/kg PCB downregulated the gene expression for T-bet, RORγ, and IFN-γ in the popliteal lymph nodes, accompanied by a significant reduction in the pathological arthritic index of AIA mice. Noteworthy, neuronal proteome analysis revealed that PCB modulated biological processes such as pain, inflammation, and glutamatergic transmission, all of which are involved in arthritic pathology.Conclusions: These findings demonstrate the remarkable efficacy of PCB in alleviating the nociception and inflammation in the AIA mice model and shed new light on mechanisms underlying the PCB modulation of the neuronal proteome. This research work opens a new avenue to explore the translational potential of PCB in developing a therapeutic strategy for inflammation and pain in rheumatoid arthritis. Fil: Marín Prida, Javier. University of Havana; Cuba Fil: Rodríguez Ulloa, Arielis. Center for Genetic Engineering and Biotechnology; Cuba Fil: Besada, Vladimir. Center for Genetic Engineering and Biotechnology; Cuba Fil: Llopiz Arzuaga, Alexey. Center for Genetic Engineering and Biotechnology; Cuba Fil: Batista, Nathália Vieira. Universidade Federal de Minas Gerais; Brasil Fil: Hernández González, Ignacio. No especifíca; Fil: Pavón Fuentes, Nancy. No especifíca; Fil: Marciano Vieira, Érica Leandro. Universidade Federal de Minas Gerais; Brasil Fil: Falcón Cama, Viviana. Center for Genetic Engineering and Biotechnology; Cuba Fil: Acosta, Emilio F.. Center for Genetic Engineering and Biotechnology; Cuba Fil: Martínez Donato, Gillian. Center for Genetic Engineering and Biotechnology; Cuba Fil: Cervantes Llanos, Majel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina Fil: Lingfeng, Dai. No especifíca; Fil: González, Luis J.. Center for Genetic Engineering and Biotechnology; Cuba Fil: Fernández Massó, Julio Raúl. Center for Genetic Engineering and Biotechnology; Cuba Fil: Guillén Nieto, Gerardo. Center for Genetic Engineering and Biotechnology; Cuba Fil: Pentón Arias, Eduardo. Center for Genetic Engineering and Biotechnology; Cuba Fil: Amaral, Flávio Almeida. Universidade Federal de Minas Gerais; Brasil Fil: Teixeira, Mauro Martins. Universidade Federal de Minas Gerais; Brasil Fil: Pentón Rol, Giselle. Center for Genetic Engineering and Biotechnology; Cuba |
| description |
Introduction: The antinociceptive and pharmacological activities of C-Phycocyanin (C-PC) and Phycocyanobilin (PCB) in the context of inflammatory arthritis remain unexplored so far. In the present study, we aimed to assess the protective actions of these compounds in an experimental mice model that replicates key aspects of human rheumatoid arthritis.Methods: Antigen-induced arthritis (AIA) was established by intradermal injection of methylated bovine serum albumin in C57BL/6 mice, and one hour before the antigen challenge, either C-PC (2, 4, or 8 mg/kg) or PCB (0.1 or 1 mg/kg) were administered intraperitoneally. Proteome profiling was also conducted on glutamate-exposed SH-SY5Y neuronal cells to evaluate the PCB impact on this key signaling pathway associated with nociceptive neuronal sensitization.Results and discussion: C-PC and PCB notably ameliorated hypernociception, synovial neutrophil infiltration, myeloperoxidase activity, and the periarticular cytokine concentration of IFN-γ, TNF-α, IL-17A, and IL-4 dose-dependently in AIA mice. In addition, 1 mg/kg PCB downregulated the gene expression for T-bet, RORγ, and IFN-γ in the popliteal lymph nodes, accompanied by a significant reduction in the pathological arthritic index of AIA mice. Noteworthy, neuronal proteome analysis revealed that PCB modulated biological processes such as pain, inflammation, and glutamatergic transmission, all of which are involved in arthritic pathology.Conclusions: These findings demonstrate the remarkable efficacy of PCB in alleviating the nociception and inflammation in the AIA mice model and shed new light on mechanisms underlying the PCB modulation of the neuronal proteome. This research work opens a new avenue to explore the translational potential of PCB in developing a therapeutic strategy for inflammation and pain in rheumatoid arthritis. |
| publishDate |
2023 |
| dc.date.none.fl_str_mv |
2023-10 |
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info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
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article |
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publishedVersion |
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http://hdl.handle.net/11336/256522 Marín Prida, Javier; Rodríguez Ulloa, Arielis; Besada, Vladimir; Llopiz Arzuaga, Alexey; Batista, Nathália Vieira; et al.; The effects of Phycocyanobilin on experimental arthritis involve the reduction in nociception and synovial neutrophil infiltration, inhibition of cytokine production, and modulation of the neuronal proteome; Frontiers Media; Frontiers in Immunology; 14; 10-2023; 1-18 1664-3224 CONICET Digital CONICET |
| url |
http://hdl.handle.net/11336/256522 |
| identifier_str_mv |
Marín Prida, Javier; Rodríguez Ulloa, Arielis; Besada, Vladimir; Llopiz Arzuaga, Alexey; Batista, Nathália Vieira; et al.; The effects of Phycocyanobilin on experimental arthritis involve the reduction in nociception and synovial neutrophil infiltration, inhibition of cytokine production, and modulation of the neuronal proteome; Frontiers Media; Frontiers in Immunology; 14; 10-2023; 1-18 1664-3224 CONICET Digital CONICET |
| dc.language.none.fl_str_mv |
eng |
| language |
eng |
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info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fimmu.2023.1227268/full info:eu-repo/semantics/altIdentifier/doi/10.3389/fimmu.2023.1227268 |
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info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
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openAccess |
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Frontiers Media |
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Frontiers Media |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
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dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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