Galectin 3 induces a distinctive pattern of cytokine and chemokine production in rheumatoid synovial fibroblasts via selective signaling pathways

Autores
Filer, Andrew D.; Bik, Magdalena; Parsonage, Greg N.; Fitton, John; Trebilcock, Emily; Howlett, Katherine; Raza, Karin; Simomns, David L.; Thomas, Andrew M.; Salmon, Mike; Scheel Toellner, Dagmar; Lord, Janet M.; Rabinovich, Gabriel Adrián; Buckley, Christopher D.
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
High expression of galectin 3 at sites of joint destruction in rheumatoid arthritis (RA) suggests that galectin 3 plays a role in RA pathogenesis. Previous studies have demonstrated the effects of galectins on immune cells, such as lymphocytes and macrophages. This study was undertaken to investigate the hypothesis that galectin 3 induces proinflammatory effects in RA by modulating the pattern of cytokine and chemokine production in synovial fibroblasts. Matched samples of RA synovial and skin fibroblasts were pretreated with galectin 3 or tumor necrosis factor alpha (TNFalpha), and the levels of a panel of cytokines, chemokines, and matrix metalloproteinases (MMPs) were determined using enzyme-linked immunosorbent assays and multiplex assays. Specific inhibitors were used to dissect signaling pathways, which were confirmed by Western blotting and NF-kappaB activation assay.
Fil: Filer, Andrew D.. University of Birmingham; Reino Unido
Fil: Bik, Magdalena. University of Birmingham; Reino Unido
Fil: Parsonage, Greg N.. University of Birmingham; Reino Unido
Fil: Fitton, John. University of Birmingham; Reino Unido
Fil: Trebilcock, Emily. University of Birmingham; Reino Unido
Fil: Howlett, Katherine. University of Birmingham; Reino Unido
Fil: Raza, Karin. University of Birmingham; Reino Unido
Fil: Simomns, David L.. Cellzome UK; Reino Unido
Fil: Thomas, Andrew M.. NHS Foundation Trust; Reino Unido
Fil: Salmon, Mike. University of Birmingham; Reino Unido
Fil: Scheel Toellner, Dagmar. University of Birmingham; Reino Unido
Fil: Lord, Janet M.. University of Birmingham; Reino Unido
Fil: Rabinovich, Gabriel Adrián. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Buckley, Christopher D.. University of Birmingham; Reino Unido
Materia
Synovia
Rheumatoid Arthritis
Galectin-3
Cytokines
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/24480
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/24480
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Galectin 3 induces a distinctive pattern of cytokine and chemokine production in rheumatoid synovial fibroblasts via selective signaling pathwaysFiler, Andrew D.Bik, MagdalenaParsonage, Greg N.Fitton, JohnTrebilcock, EmilyHowlett, KatherineRaza, KarinSimomns, David L.Thomas, Andrew M.Salmon, MikeScheel Toellner, DagmarLord, Janet M.Rabinovich, Gabriel AdriánBuckley, Christopher D.SynoviaRheumatoid ArthritisGalectin-3Cytokineshttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3https://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3High expression of galectin 3 at sites of joint destruction in rheumatoid arthritis (RA) suggests that galectin 3 plays a role in RA pathogenesis. Previous studies have demonstrated the effects of galectins on immune cells, such as lymphocytes and macrophages. This study was undertaken to investigate the hypothesis that galectin 3 induces proinflammatory effects in RA by modulating the pattern of cytokine and chemokine production in synovial fibroblasts. Matched samples of RA synovial and skin fibroblasts were pretreated with galectin 3 or tumor necrosis factor alpha (TNFalpha), and the levels of a panel of cytokines, chemokines, and matrix metalloproteinases (MMPs) were determined using enzyme-linked immunosorbent assays and multiplex assays. Specific inhibitors were used to dissect signaling pathways, which were confirmed by Western blotting and NF-kappaB activation assay.Fil: Filer, Andrew D.. University of Birmingham; Reino UnidoFil: Bik, Magdalena. University of Birmingham; Reino UnidoFil: Parsonage, Greg N.. University of Birmingham; Reino UnidoFil: Fitton, John. University of Birmingham; Reino UnidoFil: Trebilcock, Emily. University of Birmingham; Reino UnidoFil: Howlett, Katherine. University of Birmingham; Reino UnidoFil: Raza, Karin. University of Birmingham; Reino UnidoFil: Simomns, David L.. Cellzome UK; Reino UnidoFil: Thomas, Andrew M.. NHS Foundation Trust; Reino UnidoFil: Salmon, Mike. University of Birmingham; Reino UnidoFil: Scheel Toellner, Dagmar. University of Birmingham; Reino UnidoFil: Lord, Janet M.. University of Birmingham; Reino UnidoFil: Rabinovich, Gabriel Adrián. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Buckley, Christopher D.. University of Birmingham; Reino UnidoWiley2009-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/24480Filer, Andrew D.; Bik, Magdalena; Parsonage, Greg N.; Fitton, John; Trebilcock, Emily; et al.; Galectin 3 induces a distinctive pattern of cytokine and chemokine production in rheumatoid synovial fibroblasts via selective signaling pathways; Wiley; Arthritis And Rheumatism; 60; 6; 5-2009; 1604-16140004-35911529-0131CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/art.24574/abstractinfo:eu-repo/semantics/altIdentifier/doi/10.1002/art.24574info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3116228/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:51:16Zoai:ri.conicet.gov.ar:11336/24480instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:51:16.468CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Galectin 3 induces a distinctive pattern of cytokine and chemokine production in rheumatoid synovial fibroblasts via selective signaling pathways
title Galectin 3 induces a distinctive pattern of cytokine and chemokine production in rheumatoid synovial fibroblasts via selective signaling pathways
spellingShingle Galectin 3 induces a distinctive pattern of cytokine and chemokine production in rheumatoid synovial fibroblasts via selective signaling pathways
Filer, Andrew D.
Synovia
Rheumatoid Arthritis
Galectin-3
Cytokines
title_short Galectin 3 induces a distinctive pattern of cytokine and chemokine production in rheumatoid synovial fibroblasts via selective signaling pathways
title_full Galectin 3 induces a distinctive pattern of cytokine and chemokine production in rheumatoid synovial fibroblasts via selective signaling pathways
title_fullStr Galectin 3 induces a distinctive pattern of cytokine and chemokine production in rheumatoid synovial fibroblasts via selective signaling pathways
title_full_unstemmed Galectin 3 induces a distinctive pattern of cytokine and chemokine production in rheumatoid synovial fibroblasts via selective signaling pathways
title_sort Galectin 3 induces a distinctive pattern of cytokine and chemokine production in rheumatoid synovial fibroblasts via selective signaling pathways
dc.creator.none.fl_str_mv Filer, Andrew D.
Bik, Magdalena
Parsonage, Greg N.
Fitton, John
Trebilcock, Emily
Howlett, Katherine
Raza, Karin
Simomns, David L.
Thomas, Andrew M.
Salmon, Mike
Scheel Toellner, Dagmar
Lord, Janet M.
Rabinovich, Gabriel Adrián
Buckley, Christopher D.
author Filer, Andrew D.
author_facet Filer, Andrew D.
Bik, Magdalena
Parsonage, Greg N.
Fitton, John
Trebilcock, Emily
Howlett, Katherine
Raza, Karin
Simomns, David L.
Thomas, Andrew M.
Salmon, Mike
Scheel Toellner, Dagmar
Lord, Janet M.
Rabinovich, Gabriel Adrián
Buckley, Christopher D.
author_role author
author2 Bik, Magdalena
Parsonage, Greg N.
Fitton, John
Trebilcock, Emily
Howlett, Katherine
Raza, Karin
Simomns, David L.
Thomas, Andrew M.
Salmon, Mike
Scheel Toellner, Dagmar
Lord, Janet M.
Rabinovich, Gabriel Adrián
Buckley, Christopher D.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Synovia
Rheumatoid Arthritis
Galectin-3
Cytokines
topic Synovia
Rheumatoid Arthritis
Galectin-3
Cytokines
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv High expression of galectin 3 at sites of joint destruction in rheumatoid arthritis (RA) suggests that galectin 3 plays a role in RA pathogenesis. Previous studies have demonstrated the effects of galectins on immune cells, such as lymphocytes and macrophages. This study was undertaken to investigate the hypothesis that galectin 3 induces proinflammatory effects in RA by modulating the pattern of cytokine and chemokine production in synovial fibroblasts. Matched samples of RA synovial and skin fibroblasts were pretreated with galectin 3 or tumor necrosis factor alpha (TNFalpha), and the levels of a panel of cytokines, chemokines, and matrix metalloproteinases (MMPs) were determined using enzyme-linked immunosorbent assays and multiplex assays. Specific inhibitors were used to dissect signaling pathways, which were confirmed by Western blotting and NF-kappaB activation assay.
Fil: Filer, Andrew D.. University of Birmingham; Reino Unido
Fil: Bik, Magdalena. University of Birmingham; Reino Unido
Fil: Parsonage, Greg N.. University of Birmingham; Reino Unido
Fil: Fitton, John. University of Birmingham; Reino Unido
Fil: Trebilcock, Emily. University of Birmingham; Reino Unido
Fil: Howlett, Katherine. University of Birmingham; Reino Unido
Fil: Raza, Karin. University of Birmingham; Reino Unido
Fil: Simomns, David L.. Cellzome UK; Reino Unido
Fil: Thomas, Andrew M.. NHS Foundation Trust; Reino Unido
Fil: Salmon, Mike. University of Birmingham; Reino Unido
Fil: Scheel Toellner, Dagmar. University of Birmingham; Reino Unido
Fil: Lord, Janet M.. University of Birmingham; Reino Unido
Fil: Rabinovich, Gabriel Adrián. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Buckley, Christopher D.. University of Birmingham; Reino Unido
description High expression of galectin 3 at sites of joint destruction in rheumatoid arthritis (RA) suggests that galectin 3 plays a role in RA pathogenesis. Previous studies have demonstrated the effects of galectins on immune cells, such as lymphocytes and macrophages. This study was undertaken to investigate the hypothesis that galectin 3 induces proinflammatory effects in RA by modulating the pattern of cytokine and chemokine production in synovial fibroblasts. Matched samples of RA synovial and skin fibroblasts were pretreated with galectin 3 or tumor necrosis factor alpha (TNFalpha), and the levels of a panel of cytokines, chemokines, and matrix metalloproteinases (MMPs) were determined using enzyme-linked immunosorbent assays and multiplex assays. Specific inhibitors were used to dissect signaling pathways, which were confirmed by Western blotting and NF-kappaB activation assay.
publishDate 2009
dc.date.none.fl_str_mv 2009-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/24480
Filer, Andrew D.; Bik, Magdalena; Parsonage, Greg N.; Fitton, John; Trebilcock, Emily; et al.; Galectin 3 induces a distinctive pattern of cytokine and chemokine production in rheumatoid synovial fibroblasts via selective signaling pathways; Wiley; Arthritis And Rheumatism; 60; 6; 5-2009; 1604-1614
0004-3591
1529-0131
CONICET Digital
CONICET
url http://hdl.handle.net/11336/24480
identifier_str_mv Filer, Andrew D.; Bik, Magdalena; Parsonage, Greg N.; Fitton, John; Trebilcock, Emily; et al.; Galectin 3 induces a distinctive pattern of cytokine and chemokine production in rheumatoid synovial fibroblasts via selective signaling pathways; Wiley; Arthritis And Rheumatism; 60; 6; 5-2009; 1604-1614
0004-3591
1529-0131
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/art.24574/abstract
info:eu-repo/semantics/altIdentifier/doi/10.1002/art.24574
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3116228/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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