The future of CRISPR in Mycobacterium tuberculosis infection

Autores
Zein Eddine, Rima; Refrégier, Guislaine; Cervantes, Jorge; Yokobori, Noemí
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Clustered Regularly Interspaced Short Palindromic repeats (CRISPR)-Cas systems rapidly raised from a bacterial genetic curiosity to the most popular tool for genetic modifications which revolutionized the study of microbial physiology. Due to the highly conserved nature of the CRISPR locus in Mycobacterium tuberculosis, the etiological agent of one of the deadliest infectious diseases globally, initially, little attention was paid to its CRISPR locus, other than as a phylogenetic marker. Recent research shows that M. tuberculosis has a partially functional Type III CRISPR, which provides a defense mechanism against foreign genetic elements mediated by the ancillary RNAse Csm6. With the advent of CRISPR-Cas based gene edition technologies, our possibilities to explore the biology of M. tuberculosis and its interaction with the host immune system are boosted. CRISPR-based diagnostic methods can lower the detection threshold to femtomolar levels, which could contribute to the diagnosis of the still elusive paucibacillary and extrapulmonary tuberculosis cases. In addition, one-pot and point-of-care tests are under development, and future challenges are discussed. We present in this literature review the potential and actual impact of CRISPR-Cas research on human tuberculosis understanding and management. Altogether, the CRISPR-revolution will revitalize the fight against tuberculosis with more research and technological developments.
Fil: Zein Eddine, Rima. Laboratoire D'optique Et Biosciences Ecole Polytechnique; Francia
Fil: Refrégier, Guislaine. Universite Paris-saclay (universite Paris-saclay);
Fil: Cervantes, Jorge. University of Texas; Estados Unidos
Fil: Yokobori, Noemí. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas; Argentina
Materia
CRISPR-CAS
CRISPRI
DIAGNOSTIC METHODS
FUNCTIONAL GENOMICS
MTBC
MYCOBACTERIUM TUBERCULOSIS
SPOLIGOTYPING
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/219483
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/219483
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling The future of CRISPR in Mycobacterium tuberculosis infectionZein Eddine, RimaRefrégier, GuislaineCervantes, JorgeYokobori, NoemíCRISPR-CASCRISPRIDIAGNOSTIC METHODSFUNCTIONAL GENOMICSMTBCMYCOBACTERIUM TUBERCULOSISSPOLIGOTYPINGhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Clustered Regularly Interspaced Short Palindromic repeats (CRISPR)-Cas systems rapidly raised from a bacterial genetic curiosity to the most popular tool for genetic modifications which revolutionized the study of microbial physiology. Due to the highly conserved nature of the CRISPR locus in Mycobacterium tuberculosis, the etiological agent of one of the deadliest infectious diseases globally, initially, little attention was paid to its CRISPR locus, other than as a phylogenetic marker. Recent research shows that M. tuberculosis has a partially functional Type III CRISPR, which provides a defense mechanism against foreign genetic elements mediated by the ancillary RNAse Csm6. With the advent of CRISPR-Cas based gene edition technologies, our possibilities to explore the biology of M. tuberculosis and its interaction with the host immune system are boosted. CRISPR-based diagnostic methods can lower the detection threshold to femtomolar levels, which could contribute to the diagnosis of the still elusive paucibacillary and extrapulmonary tuberculosis cases. In addition, one-pot and point-of-care tests are under development, and future challenges are discussed. We present in this literature review the potential and actual impact of CRISPR-Cas research on human tuberculosis understanding and management. Altogether, the CRISPR-revolution will revitalize the fight against tuberculosis with more research and technological developments.Fil: Zein Eddine, Rima. Laboratoire D'optique Et Biosciences Ecole Polytechnique; FranciaFil: Refrégier, Guislaine. Universite Paris-saclay (universite Paris-saclay);Fil: Cervantes, Jorge. University of Texas; Estados UnidosFil: Yokobori, Noemí. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas; ArgentinaBioMed Central2023-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/219483Zein Eddine, Rima; Refrégier, Guislaine; Cervantes, Jorge; Yokobori, Noemí; The future of CRISPR in Mycobacterium tuberculosis infection; BioMed Central; Journal Of Biomedical Science; 30; 1; 5-2023; 1-121021-7770CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1186/s12929-023-00932-4info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:55:10Zoai:ri.conicet.gov.ar:11336/219483instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:55:10.491CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv The future of CRISPR in Mycobacterium tuberculosis infection
title The future of CRISPR in Mycobacterium tuberculosis infection
spellingShingle The future of CRISPR in Mycobacterium tuberculosis infection
Zein Eddine, Rima
CRISPR-CAS
CRISPRI
DIAGNOSTIC METHODS
FUNCTIONAL GENOMICS
MTBC
MYCOBACTERIUM TUBERCULOSIS
SPOLIGOTYPING
title_short The future of CRISPR in Mycobacterium tuberculosis infection
title_full The future of CRISPR in Mycobacterium tuberculosis infection
title_fullStr The future of CRISPR in Mycobacterium tuberculosis infection
title_full_unstemmed The future of CRISPR in Mycobacterium tuberculosis infection
title_sort The future of CRISPR in Mycobacterium tuberculosis infection
dc.creator.none.fl_str_mv Zein Eddine, Rima
Refrégier, Guislaine
Cervantes, Jorge
Yokobori, Noemí
author Zein Eddine, Rima
author_facet Zein Eddine, Rima
Refrégier, Guislaine
Cervantes, Jorge
Yokobori, Noemí
author_role author
author2 Refrégier, Guislaine
Cervantes, Jorge
Yokobori, Noemí
author2_role author
author
author
dc.subject.none.fl_str_mv CRISPR-CAS
CRISPRI
DIAGNOSTIC METHODS
FUNCTIONAL GENOMICS
MTBC
MYCOBACTERIUM TUBERCULOSIS
SPOLIGOTYPING
topic CRISPR-CAS
CRISPRI
DIAGNOSTIC METHODS
FUNCTIONAL GENOMICS
MTBC
MYCOBACTERIUM TUBERCULOSIS
SPOLIGOTYPING
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Clustered Regularly Interspaced Short Palindromic repeats (CRISPR)-Cas systems rapidly raised from a bacterial genetic curiosity to the most popular tool for genetic modifications which revolutionized the study of microbial physiology. Due to the highly conserved nature of the CRISPR locus in Mycobacterium tuberculosis, the etiological agent of one of the deadliest infectious diseases globally, initially, little attention was paid to its CRISPR locus, other than as a phylogenetic marker. Recent research shows that M. tuberculosis has a partially functional Type III CRISPR, which provides a defense mechanism against foreign genetic elements mediated by the ancillary RNAse Csm6. With the advent of CRISPR-Cas based gene edition technologies, our possibilities to explore the biology of M. tuberculosis and its interaction with the host immune system are boosted. CRISPR-based diagnostic methods can lower the detection threshold to femtomolar levels, which could contribute to the diagnosis of the still elusive paucibacillary and extrapulmonary tuberculosis cases. In addition, one-pot and point-of-care tests are under development, and future challenges are discussed. We present in this literature review the potential and actual impact of CRISPR-Cas research on human tuberculosis understanding and management. Altogether, the CRISPR-revolution will revitalize the fight against tuberculosis with more research and technological developments.
Fil: Zein Eddine, Rima. Laboratoire D'optique Et Biosciences Ecole Polytechnique; Francia
Fil: Refrégier, Guislaine. Universite Paris-saclay (universite Paris-saclay);
Fil: Cervantes, Jorge. University of Texas; Estados Unidos
Fil: Yokobori, Noemí. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas; Argentina
description Clustered Regularly Interspaced Short Palindromic repeats (CRISPR)-Cas systems rapidly raised from a bacterial genetic curiosity to the most popular tool for genetic modifications which revolutionized the study of microbial physiology. Due to the highly conserved nature of the CRISPR locus in Mycobacterium tuberculosis, the etiological agent of one of the deadliest infectious diseases globally, initially, little attention was paid to its CRISPR locus, other than as a phylogenetic marker. Recent research shows that M. tuberculosis has a partially functional Type III CRISPR, which provides a defense mechanism against foreign genetic elements mediated by the ancillary RNAse Csm6. With the advent of CRISPR-Cas based gene edition technologies, our possibilities to explore the biology of M. tuberculosis and its interaction with the host immune system are boosted. CRISPR-based diagnostic methods can lower the detection threshold to femtomolar levels, which could contribute to the diagnosis of the still elusive paucibacillary and extrapulmonary tuberculosis cases. In addition, one-pot and point-of-care tests are under development, and future challenges are discussed. We present in this literature review the potential and actual impact of CRISPR-Cas research on human tuberculosis understanding and management. Altogether, the CRISPR-revolution will revitalize the fight against tuberculosis with more research and technological developments.
publishDate 2023
dc.date.none.fl_str_mv 2023-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/219483
Zein Eddine, Rima; Refrégier, Guislaine; Cervantes, Jorge; Yokobori, Noemí; The future of CRISPR in Mycobacterium tuberculosis infection; BioMed Central; Journal Of Biomedical Science; 30; 1; 5-2023; 1-12
1021-7770
CONICET Digital
CONICET
url http://hdl.handle.net/11336/219483
identifier_str_mv Zein Eddine, Rima; Refrégier, Guislaine; Cervantes, Jorge; Yokobori, Noemí; The future of CRISPR in Mycobacterium tuberculosis infection; BioMed Central; Journal Of Biomedical Science; 30; 1; 5-2023; 1-12
1021-7770
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1186/s12929-023-00932-4
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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