Is Acarapis woodi mite currently infesting Apis mellifera colonies in Argentina?

Autores
Szawarski, Nicolás; Quintana, Silvina; Levy, Eugenia; Lucia, Mariano; Abrahamovich, Alberto Horacio; Porrini, Martín Pablo; Brasesco, Maria Constanza; Negri, Pedro; Sarlo, Gabriel; Eguaras, Martin Javier; Maggi, Matías Daniel
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The honey bee tracheal mite Acarapis woodi (Rennie) (Acari: Tarsonemidae) is an internal obligate parasite of the respiratory system of adult honey bees (Apis mellifera), which may be one of the factors causing the loss of bee colonies and the decrease in the beekeeping industry. In Argentina, the last prevalence study of A. woodi was in 1998, and therefore there is no current data on the prevalence and impact of the mite in colonies of A. mellifera in this country. For this reason, the objective of this study was to determine the prevalence of A. woodi in honey bee colonies of apiaries belonging to different geographic regions of Argentina (Northwest, Chaco, Mesopotamic, Pampean and Patagonic) using two different methods of diagnosis. One method was traditional microscopy, which is based on direct visualization of A. woodi in the prothoracic tracheas of bees, and the other technique was molecular analysis using real-time PCR with specific primers to amplify CO1 gene of A. woodi. To perform the analysis of prevalence, bees were collected during winter, because during this season, populations of A. woodi are more abundant in bee colonies. The results were negative for both methods of diagnosis, with prevalence equal to 0%. Considering that in recent years it has been reported an increased mortality in bee colonies in Argentina, we note that acarapisosis is not currently impacting on the disappearance of A. mellifera colonies in Argentina.
Fil: Szawarski, Nicolás. Ministerio de Ciencia, Tecnología e Innovación Productiva. Agencia Nacional de Promoción Científica y Tecnológica. Fondo para la Investigación Científica y Tecnológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina
Fil: Quintana, Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Análisis Fares Taie. Laboratorio de Biología Molecular; Argentina
Fil: Levy, Eugenia. Universidad Nacional de Mar del Plata; Argentina
Fil: Lucia, Mariano. Universidad Nacional de Mar del Plata; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Abrahamovich, Alberto Horacio. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Mar del Plata; Argentina
Fil: Porrini, Martín Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Mar del Plata; Argentina
Fil: Brasesco, Maria Constanza. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Mar del Plata; Argentina
Fil: Negri, Pedro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Mar del Plata; Argentina
Fil: Sarlo, Gabriel. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Biología; Argentina
Fil: Eguaras, Martin Javier. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Mar del Plata; Argentina
Fil: Maggi, Matías Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Mar del Plata; Argentina
Materia
Acarapis Woodi
Apis Mellifera
Argentina
Dissection Method Classic
Real-Time Pcr
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/50212
Acceder
id CONICETDig_087e6d56c0e3795c45f71c373f7c48aa
oai_identifier_str oai:ri.conicet.gov.ar:11336/50212
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Is Acarapis woodi mite currently infesting Apis mellifera colonies in Argentina?¿Está actualmente Acarapis woodi infestando colonias de Apis mellifera en Argentina?Szawarski, NicolásQuintana, SilvinaLevy, EugeniaLucia, MarianoAbrahamovich, Alberto HoracioPorrini, Martín PabloBrasesco, Maria ConstanzaNegri, PedroSarlo, GabrielEguaras, Martin JavierMaggi, Matías DanielAcarapis WoodiApis MelliferaArgentinaDissection Method ClassicReal-Time Pcrhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The honey bee tracheal mite Acarapis woodi (Rennie) (Acari: Tarsonemidae) is an internal obligate parasite of the respiratory system of adult honey bees (Apis mellifera), which may be one of the factors causing the loss of bee colonies and the decrease in the beekeeping industry. In Argentina, the last prevalence study of A. woodi was in 1998, and therefore there is no current data on the prevalence and impact of the mite in colonies of A. mellifera in this country. For this reason, the objective of this study was to determine the prevalence of A. woodi in honey bee colonies of apiaries belonging to different geographic regions of Argentina (Northwest, Chaco, Mesopotamic, Pampean and Patagonic) using two different methods of diagnosis. One method was traditional microscopy, which is based on direct visualization of A. woodi in the prothoracic tracheas of bees, and the other technique was molecular analysis using real-time PCR with specific primers to amplify CO1 gene of A. woodi. To perform the analysis of prevalence, bees were collected during winter, because during this season, populations of A. woodi are more abundant in bee colonies. The results were negative for both methods of diagnosis, with prevalence equal to 0%. Considering that in recent years it has been reported an increased mortality in bee colonies in Argentina, we note that acarapisosis is not currently impacting on the disappearance of A. mellifera colonies in Argentina.Fil: Szawarski, Nicolás. Ministerio de Ciencia, Tecnología e Innovación Productiva. Agencia Nacional de Promoción Científica y Tecnológica. Fondo para la Investigación Científica y Tecnológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; ArgentinaFil: Quintana, Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Análisis Fares Taie. Laboratorio de Biología Molecular; ArgentinaFil: Levy, Eugenia. Universidad Nacional de Mar del Plata; ArgentinaFil: Lucia, Mariano. Universidad Nacional de Mar del Plata; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Abrahamovich, Alberto Horacio. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Mar del Plata; ArgentinaFil: Porrini, Martín Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Mar del Plata; ArgentinaFil: Brasesco, Maria Constanza. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Mar del Plata; ArgentinaFil: Negri, Pedro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Mar del Plata; ArgentinaFil: Sarlo, Gabriel. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Biología; ArgentinaFil: Eguaras, Martin Javier. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Mar del Plata; ArgentinaFil: Maggi, Matías Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Mar del Plata; ArgentinaInternational Bee Research Association2017-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/50212Szawarski, Nicolás; Quintana, Silvina; Levy, Eugenia; Lucia, Mariano; Abrahamovich, Alberto Horacio; et al.; Is Acarapis woodi mite currently infesting Apis mellifera colonies in Argentina? ; International Bee Research Association; Journal Of Apicultural Research; 56; 4; 8-2017; 387-3930021-8839CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1080/00218839.2017.1339519info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:36:25Zoai:ri.conicet.gov.ar:11336/50212instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:36:25.756CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Is Acarapis woodi mite currently infesting Apis mellifera colonies in Argentina?
¿Está actualmente Acarapis woodi infestando colonias de Apis mellifera en Argentina?
title Is Acarapis woodi mite currently infesting Apis mellifera colonies in Argentina?
spellingShingle Is Acarapis woodi mite currently infesting Apis mellifera colonies in Argentina?
Szawarski, Nicolás
Acarapis Woodi
Apis Mellifera
Argentina
Dissection Method Classic
Real-Time Pcr
title_short Is Acarapis woodi mite currently infesting Apis mellifera colonies in Argentina?
title_full Is Acarapis woodi mite currently infesting Apis mellifera colonies in Argentina?
title_fullStr Is Acarapis woodi mite currently infesting Apis mellifera colonies in Argentina?
title_full_unstemmed Is Acarapis woodi mite currently infesting Apis mellifera colonies in Argentina?
title_sort Is Acarapis woodi mite currently infesting Apis mellifera colonies in Argentina?
dc.creator.none.fl_str_mv Szawarski, Nicolás
Quintana, Silvina
Levy, Eugenia
Lucia, Mariano
Abrahamovich, Alberto Horacio
Porrini, Martín Pablo
Brasesco, Maria Constanza
Negri, Pedro
Sarlo, Gabriel
Eguaras, Martin Javier
Maggi, Matías Daniel
author Szawarski, Nicolás
author_facet Szawarski, Nicolás
Quintana, Silvina
Levy, Eugenia
Lucia, Mariano
Abrahamovich, Alberto Horacio
Porrini, Martín Pablo
Brasesco, Maria Constanza
Negri, Pedro
Sarlo, Gabriel
Eguaras, Martin Javier
Maggi, Matías Daniel
author_role author
author2 Quintana, Silvina
Levy, Eugenia
Lucia, Mariano
Abrahamovich, Alberto Horacio
Porrini, Martín Pablo
Brasesco, Maria Constanza
Negri, Pedro
Sarlo, Gabriel
Eguaras, Martin Javier
Maggi, Matías Daniel
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Acarapis Woodi
Apis Mellifera
Argentina
Dissection Method Classic
Real-Time Pcr
topic Acarapis Woodi
Apis Mellifera
Argentina
Dissection Method Classic
Real-Time Pcr
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The honey bee tracheal mite Acarapis woodi (Rennie) (Acari: Tarsonemidae) is an internal obligate parasite of the respiratory system of adult honey bees (Apis mellifera), which may be one of the factors causing the loss of bee colonies and the decrease in the beekeeping industry. In Argentina, the last prevalence study of A. woodi was in 1998, and therefore there is no current data on the prevalence and impact of the mite in colonies of A. mellifera in this country. For this reason, the objective of this study was to determine the prevalence of A. woodi in honey bee colonies of apiaries belonging to different geographic regions of Argentina (Northwest, Chaco, Mesopotamic, Pampean and Patagonic) using two different methods of diagnosis. One method was traditional microscopy, which is based on direct visualization of A. woodi in the prothoracic tracheas of bees, and the other technique was molecular analysis using real-time PCR with specific primers to amplify CO1 gene of A. woodi. To perform the analysis of prevalence, bees were collected during winter, because during this season, populations of A. woodi are more abundant in bee colonies. The results were negative for both methods of diagnosis, with prevalence equal to 0%. Considering that in recent years it has been reported an increased mortality in bee colonies in Argentina, we note that acarapisosis is not currently impacting on the disappearance of A. mellifera colonies in Argentina.
Fil: Szawarski, Nicolás. Ministerio de Ciencia, Tecnología e Innovación Productiva. Agencia Nacional de Promoción Científica y Tecnológica. Fondo para la Investigación Científica y Tecnológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina
Fil: Quintana, Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Análisis Fares Taie. Laboratorio de Biología Molecular; Argentina
Fil: Levy, Eugenia. Universidad Nacional de Mar del Plata; Argentina
Fil: Lucia, Mariano. Universidad Nacional de Mar del Plata; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Abrahamovich, Alberto Horacio. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Mar del Plata; Argentina
Fil: Porrini, Martín Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Mar del Plata; Argentina
Fil: Brasesco, Maria Constanza. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Mar del Plata; Argentina
Fil: Negri, Pedro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Mar del Plata; Argentina
Fil: Sarlo, Gabriel. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Biología; Argentina
Fil: Eguaras, Martin Javier. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Mar del Plata; Argentina
Fil: Maggi, Matías Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Mar del Plata; Argentina
description The honey bee tracheal mite Acarapis woodi (Rennie) (Acari: Tarsonemidae) is an internal obligate parasite of the respiratory system of adult honey bees (Apis mellifera), which may be one of the factors causing the loss of bee colonies and the decrease in the beekeeping industry. In Argentina, the last prevalence study of A. woodi was in 1998, and therefore there is no current data on the prevalence and impact of the mite in colonies of A. mellifera in this country. For this reason, the objective of this study was to determine the prevalence of A. woodi in honey bee colonies of apiaries belonging to different geographic regions of Argentina (Northwest, Chaco, Mesopotamic, Pampean and Patagonic) using two different methods of diagnosis. One method was traditional microscopy, which is based on direct visualization of A. woodi in the prothoracic tracheas of bees, and the other technique was molecular analysis using real-time PCR with specific primers to amplify CO1 gene of A. woodi. To perform the analysis of prevalence, bees were collected during winter, because during this season, populations of A. woodi are more abundant in bee colonies. The results were negative for both methods of diagnosis, with prevalence equal to 0%. Considering that in recent years it has been reported an increased mortality in bee colonies in Argentina, we note that acarapisosis is not currently impacting on the disappearance of A. mellifera colonies in Argentina.
publishDate 2017
dc.date.none.fl_str_mv 2017-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/50212
Szawarski, Nicolás; Quintana, Silvina; Levy, Eugenia; Lucia, Mariano; Abrahamovich, Alberto Horacio; et al.; Is Acarapis woodi mite currently infesting Apis mellifera colonies in Argentina? ; International Bee Research Association; Journal Of Apicultural Research; 56; 4; 8-2017; 387-393
0021-8839
CONICET Digital
CONICET
url http://hdl.handle.net/11336/50212
identifier_str_mv Szawarski, Nicolás; Quintana, Silvina; Levy, Eugenia; Lucia, Mariano; Abrahamovich, Alberto Horacio; et al.; Is Acarapis woodi mite currently infesting Apis mellifera colonies in Argentina? ; International Bee Research Association; Journal Of Apicultural Research; 56; 4; 8-2017; 387-393
0021-8839
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1080/00218839.2017.1339519
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv International Bee Research Association
publisher.none.fl_str_mv International Bee Research Association
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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