Comparison of qPCR and Morphological Methods For Detection of Acarapis Woodi in Honey Bee Samples
- Autores
- Quintana, Silvina; Szawarski, Nicolás; Sarlo, Gabriel; Medici, Sandra; Rivero, Mariana; Eguaras, Martín Javier; Maggi, Matías
- Año de publicación
- 2019
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión enviada
- Descripción
- The honey bee tracheal mite Acarapis woodi is an internal obligate parasite of adult honey bees (Apis mellifera). The small size of the mites and location within the bee’s trachea create a challenge for diagnostic identification. Detailed assessment of low-level mite infestation involves microscopic examination of the bee’s tracheae, but this traditional diagnostic method takes a long time. In recent years, new molecular diagnostic techniques have been developed to identify A. woodi with the use of PCRs. The purpose of this study was to compare the diagnostic sensitivity of microscopic examination and qPCR method for the detection of A. woodi in honey bee samples. Thirty-six samples were analyzed with both methods and qPCR was more sensitive than the microscopic method to detect A. woodi. This work demonstrates the practical application of molecular technology as a support tool for surveys and contingency management and to provide robust surveillance data on the presence or absence of A. woodi in honey bee colonies.
- Materia
-
Biología Celular, Microbiología
Acarapis woodi
Apis mellifera
Internal control
Optical microscopy diagnosis
Real time PCR - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by/4.0/
- Repositorio
- Institución
- Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
- OAI Identificador
- oai:digital.cic.gba.gob.ar:11746/11362
Ver los metadatos del registro completo
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Comparison of qPCR and Morphological Methods For Detection of Acarapis Woodi in Honey Bee SamplesQuintana, SilvinaSzawarski, NicolásSarlo, GabrielMedici, SandraRivero, MarianaEguaras, Martín JavierMaggi, MatíasBiología Celular, MicrobiologíaAcarapis woodiApis melliferaInternal controlOptical microscopy diagnosisReal time PCRThe honey bee tracheal mite Acarapis woodi is an internal obligate parasite of adult honey bees (Apis mellifera). The small size of the mites and location within the bee’s trachea create a challenge for diagnostic identification. Detailed assessment of low-level mite infestation involves microscopic examination of the bee’s tracheae, but this traditional diagnostic method takes a long time. In recent years, new molecular diagnostic techniques have been developed to identify A. woodi with the use of PCRs. The purpose of this study was to compare the diagnostic sensitivity of microscopic examination and qPCR method for the detection of A. woodi in honey bee samples. Thirty-six samples were analyzed with both methods and qPCR was more sensitive than the microscopic method to detect A. woodi. This work demonstrates the practical application of molecular technology as a support tool for surveys and contingency management and to provide robust surveillance data on the presence or absence of A. woodi in honey bee colonies.2019info:eu-repo/semantics/articleinfo:eu-repo/semantics/submittedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://digital.cic.gba.gob.ar/handle/11746/11362enginfo:eu-repo/semantics/altIdentifier/doi/10.2478/jas-2019-0011info:eu-repo/semantics/altIdentifier/issn/2299-4831info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/reponame:CIC Digital (CICBA)instname:Comisión de Investigaciones Científicas de la Provincia de Buenos Airesinstacron:CICBA2025-09-29T13:40:17Zoai:digital.cic.gba.gob.ar:11746/11362Institucionalhttp://digital.cic.gba.gob.arOrganismo científico-tecnológicoNo correspondehttp://digital.cic.gba.gob.ar/oai/snrdmarisa.degiusti@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:94412025-09-29 13:40:17.905CIC Digital (CICBA) - Comisión de Investigaciones Científicas de la Provincia de Buenos Airesfalse |
dc.title.none.fl_str_mv |
Comparison of qPCR and Morphological Methods For Detection of Acarapis Woodi in Honey Bee Samples |
title |
Comparison of qPCR and Morphological Methods For Detection of Acarapis Woodi in Honey Bee Samples |
spellingShingle |
Comparison of qPCR and Morphological Methods For Detection of Acarapis Woodi in Honey Bee Samples Quintana, Silvina Biología Celular, Microbiología Acarapis woodi Apis mellifera Internal control Optical microscopy diagnosis Real time PCR |
title_short |
Comparison of qPCR and Morphological Methods For Detection of Acarapis Woodi in Honey Bee Samples |
title_full |
Comparison of qPCR and Morphological Methods For Detection of Acarapis Woodi in Honey Bee Samples |
title_fullStr |
Comparison of qPCR and Morphological Methods For Detection of Acarapis Woodi in Honey Bee Samples |
title_full_unstemmed |
Comparison of qPCR and Morphological Methods For Detection of Acarapis Woodi in Honey Bee Samples |
title_sort |
Comparison of qPCR and Morphological Methods For Detection of Acarapis Woodi in Honey Bee Samples |
dc.creator.none.fl_str_mv |
Quintana, Silvina Szawarski, Nicolás Sarlo, Gabriel Medici, Sandra Rivero, Mariana Eguaras, Martín Javier Maggi, Matías |
author |
Quintana, Silvina |
author_facet |
Quintana, Silvina Szawarski, Nicolás Sarlo, Gabriel Medici, Sandra Rivero, Mariana Eguaras, Martín Javier Maggi, Matías |
author_role |
author |
author2 |
Szawarski, Nicolás Sarlo, Gabriel Medici, Sandra Rivero, Mariana Eguaras, Martín Javier Maggi, Matías |
author2_role |
author author author author author author |
dc.subject.none.fl_str_mv |
Biología Celular, Microbiología Acarapis woodi Apis mellifera Internal control Optical microscopy diagnosis Real time PCR |
topic |
Biología Celular, Microbiología Acarapis woodi Apis mellifera Internal control Optical microscopy diagnosis Real time PCR |
dc.description.none.fl_txt_mv |
The honey bee tracheal mite Acarapis woodi is an internal obligate parasite of adult honey bees (Apis mellifera). The small size of the mites and location within the bee’s trachea create a challenge for diagnostic identification. Detailed assessment of low-level mite infestation involves microscopic examination of the bee’s tracheae, but this traditional diagnostic method takes a long time. In recent years, new molecular diagnostic techniques have been developed to identify A. woodi with the use of PCRs. The purpose of this study was to compare the diagnostic sensitivity of microscopic examination and qPCR method for the detection of A. woodi in honey bee samples. Thirty-six samples were analyzed with both methods and qPCR was more sensitive than the microscopic method to detect A. woodi. This work demonstrates the practical application of molecular technology as a support tool for surveys and contingency management and to provide robust surveillance data on the presence or absence of A. woodi in honey bee colonies. |
description |
The honey bee tracheal mite Acarapis woodi is an internal obligate parasite of adult honey bees (Apis mellifera). The small size of the mites and location within the bee’s trachea create a challenge for diagnostic identification. Detailed assessment of low-level mite infestation involves microscopic examination of the bee’s tracheae, but this traditional diagnostic method takes a long time. In recent years, new molecular diagnostic techniques have been developed to identify A. woodi with the use of PCRs. The purpose of this study was to compare the diagnostic sensitivity of microscopic examination and qPCR method for the detection of A. woodi in honey bee samples. Thirty-six samples were analyzed with both methods and qPCR was more sensitive than the microscopic method to detect A. woodi. This work demonstrates the practical application of molecular technology as a support tool for surveys and contingency management and to provide robust surveillance data on the presence or absence of A. woodi in honey bee colonies. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/submittedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
submittedVersion |
dc.identifier.none.fl_str_mv |
https://digital.cic.gba.gob.ar/handle/11746/11362 |
url |
https://digital.cic.gba.gob.ar/handle/11746/11362 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.2478/jas-2019-0011 info:eu-repo/semantics/altIdentifier/issn/2299-4831 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/4.0/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by/4.0/ |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:CIC Digital (CICBA) instname:Comisión de Investigaciones Científicas de la Provincia de Buenos Aires instacron:CICBA |
reponame_str |
CIC Digital (CICBA) |
collection |
CIC Digital (CICBA) |
instname_str |
Comisión de Investigaciones Científicas de la Provincia de Buenos Aires |
instacron_str |
CICBA |
institution |
CICBA |
repository.name.fl_str_mv |
CIC Digital (CICBA) - Comisión de Investigaciones Científicas de la Provincia de Buenos Aires |
repository.mail.fl_str_mv |
marisa.degiusti@sedici.unlp.edu.ar |
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score |
13.070432 |