Comparison of qPCR and Morphological Methods For Detection of Acarapis Woodi in Honey Bee Samples

Autores
Quintana, Silvina; Szawarski, Nicolás; Sarlo, Gabriel; Medici, Sandra; Rivero, Mariana; Eguaras, Martín Javier; Maggi, Matías
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión enviada
Descripción
The honey bee tracheal mite Acarapis woodi is an internal obligate parasite of adult honey bees (Apis mellifera). The small size of the mites and location within the bee’s trachea create a challenge for diagnostic identification. Detailed assessment of low-level mite infestation involves microscopic examination of the bee’s tracheae, but this traditional diagnostic method takes a long time. In recent years, new molecular diagnostic techniques have been developed to identify A. woodi with the use of PCRs. The purpose of this study was to compare the diagnostic sensitivity of microscopic examination and qPCR method for the detection of A. woodi in honey bee samples. Thirty-six samples were analyzed with both methods and qPCR was more sensitive than the microscopic method to detect A. woodi. This work demonstrates the practical application of molecular technology as a support tool for surveys and contingency management and to provide robust surveillance data on the presence or absence of A. woodi in honey bee colonies.
Materia
Biología Celular, Microbiología
Acarapis woodi
Apis mellifera
Internal control
Optical microscopy diagnosis
Real time PCR
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
CIC Digital (CICBA)
Institución
Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
OAI Identificador
oai:digital.cic.gba.gob.ar:11746/11362

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network_acronym_str CICBA
repository_id_str 9441
network_name_str CIC Digital (CICBA)
spelling Comparison of qPCR and Morphological Methods For Detection of Acarapis Woodi in Honey Bee SamplesQuintana, SilvinaSzawarski, NicolásSarlo, GabrielMedici, SandraRivero, MarianaEguaras, Martín JavierMaggi, MatíasBiología Celular, MicrobiologíaAcarapis woodiApis melliferaInternal controlOptical microscopy diagnosisReal time PCRThe honey bee tracheal mite Acarapis woodi is an internal obligate parasite of adult honey bees (Apis mellifera). The small size of the mites and location within the bee’s trachea create a challenge for diagnostic identification. Detailed assessment of low-level mite infestation involves microscopic examination of the bee’s tracheae, but this traditional diagnostic method takes a long time. In recent years, new molecular diagnostic techniques have been developed to identify A. woodi with the use of PCRs. The purpose of this study was to compare the diagnostic sensitivity of microscopic examination and qPCR method for the detection of A. woodi in honey bee samples. Thirty-six samples were analyzed with both methods and qPCR was more sensitive than the microscopic method to detect A. woodi. This work demonstrates the practical application of molecular technology as a support tool for surveys and contingency management and to provide robust surveillance data on the presence or absence of A. woodi in honey bee colonies.2019info:eu-repo/semantics/articleinfo:eu-repo/semantics/submittedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://digital.cic.gba.gob.ar/handle/11746/11362enginfo:eu-repo/semantics/altIdentifier/doi/10.2478/jas-2019-0011info:eu-repo/semantics/altIdentifier/issn/2299-4831info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/reponame:CIC Digital (CICBA)instname:Comisión de Investigaciones Científicas de la Provincia de Buenos Airesinstacron:CICBA2025-09-29T13:40:17Zoai:digital.cic.gba.gob.ar:11746/11362Institucionalhttp://digital.cic.gba.gob.arOrganismo científico-tecnológicoNo correspondehttp://digital.cic.gba.gob.ar/oai/snrdmarisa.degiusti@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:94412025-09-29 13:40:17.905CIC Digital (CICBA) - Comisión de Investigaciones Científicas de la Provincia de Buenos Airesfalse
dc.title.none.fl_str_mv Comparison of qPCR and Morphological Methods For Detection of Acarapis Woodi in Honey Bee Samples
title Comparison of qPCR and Morphological Methods For Detection of Acarapis Woodi in Honey Bee Samples
spellingShingle Comparison of qPCR and Morphological Methods For Detection of Acarapis Woodi in Honey Bee Samples
Quintana, Silvina
Biología Celular, Microbiología
Acarapis woodi
Apis mellifera
Internal control
Optical microscopy diagnosis
Real time PCR
title_short Comparison of qPCR and Morphological Methods For Detection of Acarapis Woodi in Honey Bee Samples
title_full Comparison of qPCR and Morphological Methods For Detection of Acarapis Woodi in Honey Bee Samples
title_fullStr Comparison of qPCR and Morphological Methods For Detection of Acarapis Woodi in Honey Bee Samples
title_full_unstemmed Comparison of qPCR and Morphological Methods For Detection of Acarapis Woodi in Honey Bee Samples
title_sort Comparison of qPCR and Morphological Methods For Detection of Acarapis Woodi in Honey Bee Samples
dc.creator.none.fl_str_mv Quintana, Silvina
Szawarski, Nicolás
Sarlo, Gabriel
Medici, Sandra
Rivero, Mariana
Eguaras, Martín Javier
Maggi, Matías
author Quintana, Silvina
author_facet Quintana, Silvina
Szawarski, Nicolás
Sarlo, Gabriel
Medici, Sandra
Rivero, Mariana
Eguaras, Martín Javier
Maggi, Matías
author_role author
author2 Szawarski, Nicolás
Sarlo, Gabriel
Medici, Sandra
Rivero, Mariana
Eguaras, Martín Javier
Maggi, Matías
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Biología Celular, Microbiología
Acarapis woodi
Apis mellifera
Internal control
Optical microscopy diagnosis
Real time PCR
topic Biología Celular, Microbiología
Acarapis woodi
Apis mellifera
Internal control
Optical microscopy diagnosis
Real time PCR
dc.description.none.fl_txt_mv The honey bee tracheal mite Acarapis woodi is an internal obligate parasite of adult honey bees (Apis mellifera). The small size of the mites and location within the bee’s trachea create a challenge for diagnostic identification. Detailed assessment of low-level mite infestation involves microscopic examination of the bee’s tracheae, but this traditional diagnostic method takes a long time. In recent years, new molecular diagnostic techniques have been developed to identify A. woodi with the use of PCRs. The purpose of this study was to compare the diagnostic sensitivity of microscopic examination and qPCR method for the detection of A. woodi in honey bee samples. Thirty-six samples were analyzed with both methods and qPCR was more sensitive than the microscopic method to detect A. woodi. This work demonstrates the practical application of molecular technology as a support tool for surveys and contingency management and to provide robust surveillance data on the presence or absence of A. woodi in honey bee colonies.
description The honey bee tracheal mite Acarapis woodi is an internal obligate parasite of adult honey bees (Apis mellifera). The small size of the mites and location within the bee’s trachea create a challenge for diagnostic identification. Detailed assessment of low-level mite infestation involves microscopic examination of the bee’s tracheae, but this traditional diagnostic method takes a long time. In recent years, new molecular diagnostic techniques have been developed to identify A. woodi with the use of PCRs. The purpose of this study was to compare the diagnostic sensitivity of microscopic examination and qPCR method for the detection of A. woodi in honey bee samples. Thirty-six samples were analyzed with both methods and qPCR was more sensitive than the microscopic method to detect A. woodi. This work demonstrates the practical application of molecular technology as a support tool for surveys and contingency management and to provide robust surveillance data on the presence or absence of A. woodi in honey bee colonies.
publishDate 2019
dc.date.none.fl_str_mv 2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/submittedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str submittedVersion
dc.identifier.none.fl_str_mv https://digital.cic.gba.gob.ar/handle/11746/11362
url https://digital.cic.gba.gob.ar/handle/11746/11362
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.2478/jas-2019-0011
info:eu-repo/semantics/altIdentifier/issn/2299-4831
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:CIC Digital (CICBA)
instname:Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
instacron:CICBA
reponame_str CIC Digital (CICBA)
collection CIC Digital (CICBA)
instname_str Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
instacron_str CICBA
institution CICBA
repository.name.fl_str_mv CIC Digital (CICBA) - Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
repository.mail.fl_str_mv marisa.degiusti@sedici.unlp.edu.ar
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