Enhacement of a solid culture medium for chlamydospore production of Duddingtonia flagrans

Autores
Southwell, Manuela; Junco, Milagros; Fernández, Alicia Silvina; Bernat, Gisele Anahí; Zegbi, Sara; Guerrero, Ines; Sagüés, María Federica
Año de publicación
2025
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Duddingtonia flagrans is a microfungus used for the biological control of gastrointestinal nematodes in ruminants, which negatively impacts livestock production. This study aimed to evaluate chlamydospore production in different culture media and assess its impact on nematode predatory capacity. D. flagrans was cultured in enriched Glucose Sabouraud Agar (GSA-E) with the addition of meso-inositol (MI) and mannitol (M) at different concentrations: GSA-E+MI1.1%+M2%, GSA-E+MI1.1%+M5%, GSA-E+MI2%+M2%, GSA-E+MI2%M5% and GSA only (control). Twelve plates per concentration were incubated for 3, 4, and 5 weeks, respectively, at 27±0.5°C and 70±5% RH. Chlamydospores were quantified under optical microscope after mycelium homogenization. Nematophagous activity was assessed in faecal cultures with faeces from naturally parasitised sheep, incubated for 15 days at 24ºC. Infective larvae (L3) were recovered by baermannisation, quantified, and their reduction was calculated. Data were expressed as mean ±SEM. Normality was evaluated using the Shapiro-Wilk test. Group comparisons were performed using One-Way ANOVA and Dunnett’s tests or Kruskal-Wallis and Dunn’s tests. At 3, 4, and 5 weeks, respectively, the values were: GSA: 8.4×10⁶ (±1.1×10⁶), 8.2×10⁶ (±1.4×10⁶), 8.9×10⁶ (±1.2×10⁶); GSA-E+MI1.1%+M2%: 2.9×10⁷ (±4.8×106), 3.5×10⁷ (±3.4×106), 2.6×10⁷ (±4.3×106); GSA-E+MI1.1%+M5%: 3.2×10⁷ (±3.6×106), 8.7×10⁶ (±3.0×10⁶), 2.6×10⁷ (±6.6×106); GSA-E+MI2%+M2%: 8.9×10⁶ (±3.6×10⁶), 5.4×10⁶ (±1.6×10⁶), 1.7×10⁷ (±5.2×106); GSA-E+MI2%+M5%: 1.7×10⁷ (±5.9×106), 4.1×10⁷ (±8.6×106), 6.9×10⁷ (±1.3×10⁷). The GSA-E+MI2%+M5% medium optimised chlamydospore production at 5 weeks. The numbers of L3 recovered from MI- and M-enriched media were 74-99.7% (p<0.05) lower than in the control group. Supplementation with MI and M enhances chlamydospore production of D. flagrans without compromising its predatory capacity, thus optimising its production to use it in biological control against nematodes.
Fil: Southwell, Manuela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Junco, Milagros. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Fernández, Alicia Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Bernat, Gisele Anahí. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Zegbi, Sara. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Guerrero, Ines. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Sanidad Animal y Medicina Preventiva. Área Parasitología y Enfermedades Parasitarias; Argentina
Fil: Sagüés, María Federica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
30th Conference of the World Association for the Advancement of Veterinary Parasitology
Curitiba
Brasil
World Association for the Advancement of Veterinary Parasitology
Materia
OPTIMISED CULTURE MEDIA
MESO-INOSITOL
MANNITOL
CHLAMYDOSPORES
DUDDINGTONIA FLAGRANS
BIOLOGICAL CONTROL
GASTROINTESTINAL NEMATODES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/280025
Acceder
id CONICETDig_08305d488628f41ebeee53b01839e27a
oai_identifier_str oai:ri.conicet.gov.ar:11336/280025
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Enhacement of a solid culture medium for chlamydospore production of Duddingtonia flagransSouthwell, ManuelaJunco, MilagrosFernández, Alicia SilvinaBernat, Gisele AnahíZegbi, SaraGuerrero, InesSagüés, María FedericaOPTIMISED CULTURE MEDIAMESO-INOSITOLMANNITOLCHLAMYDOSPORESDUDDINGTONIA FLAGRANSBIOLOGICAL CONTROLGASTROINTESTINAL NEMATODEShttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Duddingtonia flagrans is a microfungus used for the biological control of gastrointestinal nematodes in ruminants, which negatively impacts livestock production. This study aimed to evaluate chlamydospore production in different culture media and assess its impact on nematode predatory capacity. D. flagrans was cultured in enriched Glucose Sabouraud Agar (GSA-E) with the addition of meso-inositol (MI) and mannitol (M) at different concentrations: GSA-E+MI1.1%+M2%, GSA-E+MI1.1%+M5%, GSA-E+MI2%+M2%, GSA-E+MI2%M5% and GSA only (control). Twelve plates per concentration were incubated for 3, 4, and 5 weeks, respectively, at 27±0.5°C and 70±5% RH. Chlamydospores were quantified under optical microscope after mycelium homogenization. Nematophagous activity was assessed in faecal cultures with faeces from naturally parasitised sheep, incubated for 15 days at 24ºC. Infective larvae (L3) were recovered by baermannisation, quantified, and their reduction was calculated. Data were expressed as mean ±SEM. Normality was evaluated using the Shapiro-Wilk test. Group comparisons were performed using One-Way ANOVA and Dunnett’s tests or Kruskal-Wallis and Dunn’s tests. At 3, 4, and 5 weeks, respectively, the values were: GSA: 8.4×10⁶ (±1.1×10⁶), 8.2×10⁶ (±1.4×10⁶), 8.9×10⁶ (±1.2×10⁶); GSA-E+MI1.1%+M2%: 2.9×10⁷ (±4.8×106), 3.5×10⁷ (±3.4×106), 2.6×10⁷ (±4.3×106); GSA-E+MI1.1%+M5%: 3.2×10⁷ (±3.6×106), 8.7×10⁶ (±3.0×10⁶), 2.6×10⁷ (±6.6×106); GSA-E+MI2%+M2%: 8.9×10⁶ (±3.6×10⁶), 5.4×10⁶ (±1.6×10⁶), 1.7×10⁷ (±5.2×106); GSA-E+MI2%+M5%: 1.7×10⁷ (±5.9×106), 4.1×10⁷ (±8.6×106), 6.9×10⁷ (±1.3×10⁷). The GSA-E+MI2%+M5% medium optimised chlamydospore production at 5 weeks. The numbers of L3 recovered from MI- and M-enriched media were 74-99.7% (p<0.05) lower than in the control group. Supplementation with MI and M enhances chlamydospore production of D. flagrans without compromising its predatory capacity, thus optimising its production to use it in biological control against nematodes.Fil: Southwell, Manuela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; ArgentinaFil: Junco, Milagros. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; ArgentinaFil: Fernández, Alicia Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; ArgentinaFil: Bernat, Gisele Anahí. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; ArgentinaFil: Zegbi, Sara. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; ArgentinaFil: Guerrero, Ines. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Sanidad Animal y Medicina Preventiva. Área Parasitología y Enfermedades Parasitarias; ArgentinaFil: Sagüés, María Federica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina30th Conference of the World Association for the Advancement of Veterinary ParasitologyCuritibaBrasilWorld Association for the Advancement of Veterinary ParasitologyWorld Association for the Advancement of Veterinary Parasitology2025info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectConferenciaBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/280025Enhacement of a solid culture medium for chlamydospore production of Duddingtonia flagrans; 30th Conference of the World Association for the Advancement of Veterinary Parasitology; Curitiba; Brasil; 2025; 199-200CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.waavp2025.comInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-02-06T12:03:10Zoai:ri.conicet.gov.ar:11336/280025instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-02-06 12:03:10.858CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Enhacement of a solid culture medium for chlamydospore production of Duddingtonia flagrans
title Enhacement of a solid culture medium for chlamydospore production of Duddingtonia flagrans
spellingShingle Enhacement of a solid culture medium for chlamydospore production of Duddingtonia flagrans
Southwell, Manuela
OPTIMISED CULTURE MEDIA
MESO-INOSITOL
MANNITOL
CHLAMYDOSPORES
DUDDINGTONIA FLAGRANS
BIOLOGICAL CONTROL
GASTROINTESTINAL NEMATODES
title_short Enhacement of a solid culture medium for chlamydospore production of Duddingtonia flagrans
title_full Enhacement of a solid culture medium for chlamydospore production of Duddingtonia flagrans
title_fullStr Enhacement of a solid culture medium for chlamydospore production of Duddingtonia flagrans
title_full_unstemmed Enhacement of a solid culture medium for chlamydospore production of Duddingtonia flagrans
title_sort Enhacement of a solid culture medium for chlamydospore production of Duddingtonia flagrans
dc.creator.none.fl_str_mv Southwell, Manuela
Junco, Milagros
Fernández, Alicia Silvina
Bernat, Gisele Anahí
Zegbi, Sara
Guerrero, Ines
Sagüés, María Federica
author Southwell, Manuela
author_facet Southwell, Manuela
Junco, Milagros
Fernández, Alicia Silvina
Bernat, Gisele Anahí
Zegbi, Sara
Guerrero, Ines
Sagüés, María Federica
author_role author
author2 Junco, Milagros
Fernández, Alicia Silvina
Bernat, Gisele Anahí
Zegbi, Sara
Guerrero, Ines
Sagüés, María Federica
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv OPTIMISED CULTURE MEDIA
MESO-INOSITOL
MANNITOL
CHLAMYDOSPORES
DUDDINGTONIA FLAGRANS
BIOLOGICAL CONTROL
GASTROINTESTINAL NEMATODES
topic OPTIMISED CULTURE MEDIA
MESO-INOSITOL
MANNITOL
CHLAMYDOSPORES
DUDDINGTONIA FLAGRANS
BIOLOGICAL CONTROL
GASTROINTESTINAL NEMATODES
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Duddingtonia flagrans is a microfungus used for the biological control of gastrointestinal nematodes in ruminants, which negatively impacts livestock production. This study aimed to evaluate chlamydospore production in different culture media and assess its impact on nematode predatory capacity. D. flagrans was cultured in enriched Glucose Sabouraud Agar (GSA-E) with the addition of meso-inositol (MI) and mannitol (M) at different concentrations: GSA-E+MI1.1%+M2%, GSA-E+MI1.1%+M5%, GSA-E+MI2%+M2%, GSA-E+MI2%M5% and GSA only (control). Twelve plates per concentration were incubated for 3, 4, and 5 weeks, respectively, at 27±0.5°C and 70±5% RH. Chlamydospores were quantified under optical microscope after mycelium homogenization. Nematophagous activity was assessed in faecal cultures with faeces from naturally parasitised sheep, incubated for 15 days at 24ºC. Infective larvae (L3) were recovered by baermannisation, quantified, and their reduction was calculated. Data were expressed as mean ±SEM. Normality was evaluated using the Shapiro-Wilk test. Group comparisons were performed using One-Way ANOVA and Dunnett’s tests or Kruskal-Wallis and Dunn’s tests. At 3, 4, and 5 weeks, respectively, the values were: GSA: 8.4×10⁶ (±1.1×10⁶), 8.2×10⁶ (±1.4×10⁶), 8.9×10⁶ (±1.2×10⁶); GSA-E+MI1.1%+M2%: 2.9×10⁷ (±4.8×106), 3.5×10⁷ (±3.4×106), 2.6×10⁷ (±4.3×106); GSA-E+MI1.1%+M5%: 3.2×10⁷ (±3.6×106), 8.7×10⁶ (±3.0×10⁶), 2.6×10⁷ (±6.6×106); GSA-E+MI2%+M2%: 8.9×10⁶ (±3.6×10⁶), 5.4×10⁶ (±1.6×10⁶), 1.7×10⁷ (±5.2×106); GSA-E+MI2%+M5%: 1.7×10⁷ (±5.9×106), 4.1×10⁷ (±8.6×106), 6.9×10⁷ (±1.3×10⁷). The GSA-E+MI2%+M5% medium optimised chlamydospore production at 5 weeks. The numbers of L3 recovered from MI- and M-enriched media were 74-99.7% (p<0.05) lower than in the control group. Supplementation with MI and M enhances chlamydospore production of D. flagrans without compromising its predatory capacity, thus optimising its production to use it in biological control against nematodes.
Fil: Southwell, Manuela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Junco, Milagros. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Fernández, Alicia Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Bernat, Gisele Anahí. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Zegbi, Sara. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Guerrero, Ines. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Sanidad Animal y Medicina Preventiva. Área Parasitología y Enfermedades Parasitarias; Argentina
Fil: Sagüés, María Federica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
30th Conference of the World Association for the Advancement of Veterinary Parasitology
Curitiba
Brasil
World Association for the Advancement of Veterinary Parasitology
description Duddingtonia flagrans is a microfungus used for the biological control of gastrointestinal nematodes in ruminants, which negatively impacts livestock production. This study aimed to evaluate chlamydospore production in different culture media and assess its impact on nematode predatory capacity. D. flagrans was cultured in enriched Glucose Sabouraud Agar (GSA-E) with the addition of meso-inositol (MI) and mannitol (M) at different concentrations: GSA-E+MI1.1%+M2%, GSA-E+MI1.1%+M5%, GSA-E+MI2%+M2%, GSA-E+MI2%M5% and GSA only (control). Twelve plates per concentration were incubated for 3, 4, and 5 weeks, respectively, at 27±0.5°C and 70±5% RH. Chlamydospores were quantified under optical microscope after mycelium homogenization. Nematophagous activity was assessed in faecal cultures with faeces from naturally parasitised sheep, incubated for 15 days at 24ºC. Infective larvae (L3) were recovered by baermannisation, quantified, and their reduction was calculated. Data were expressed as mean ±SEM. Normality was evaluated using the Shapiro-Wilk test. Group comparisons were performed using One-Way ANOVA and Dunnett’s tests or Kruskal-Wallis and Dunn’s tests. At 3, 4, and 5 weeks, respectively, the values were: GSA: 8.4×10⁶ (±1.1×10⁶), 8.2×10⁶ (±1.4×10⁶), 8.9×10⁶ (±1.2×10⁶); GSA-E+MI1.1%+M2%: 2.9×10⁷ (±4.8×106), 3.5×10⁷ (±3.4×106), 2.6×10⁷ (±4.3×106); GSA-E+MI1.1%+M5%: 3.2×10⁷ (±3.6×106), 8.7×10⁶ (±3.0×10⁶), 2.6×10⁷ (±6.6×106); GSA-E+MI2%+M2%: 8.9×10⁶ (±3.6×10⁶), 5.4×10⁶ (±1.6×10⁶), 1.7×10⁷ (±5.2×106); GSA-E+MI2%+M5%: 1.7×10⁷ (±5.9×106), 4.1×10⁷ (±8.6×106), 6.9×10⁷ (±1.3×10⁷). The GSA-E+MI2%+M5% medium optimised chlamydospore production at 5 weeks. The numbers of L3 recovered from MI- and M-enriched media were 74-99.7% (p<0.05) lower than in the control group. Supplementation with MI and M enhances chlamydospore production of D. flagrans without compromising its predatory capacity, thus optimising its production to use it in biological control against nematodes.
publishDate 2025
dc.date.none.fl_str_mv 2025
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Conferencia
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/280025
Enhacement of a solid culture medium for chlamydospore production of Duddingtonia flagrans; 30th Conference of the World Association for the Advancement of Veterinary Parasitology; Curitiba; Brasil; 2025; 199-200
CONICET Digital
CONICET
url http://hdl.handle.net/11336/280025
identifier_str_mv Enhacement of a solid culture medium for chlamydospore production of Duddingtonia flagrans; 30th Conference of the World Association for the Advancement of Veterinary Parasitology; Curitiba; Brasil; 2025; 199-200
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.waavp2025.com
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv World Association for the Advancement of Veterinary Parasitology
publisher.none.fl_str_mv World Association for the Advancement of Veterinary Parasitology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1856402749558620160
score 13.106097

Publicaciones similares