Bacterial indicators and antibiotic resistance of Escherichia coli in groundwater

Autores
Gambero, Maria Laura; Blarasin, Mónica Teresa; Bettera, Susana Gertrudis; Matteoda, Edel Mara
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The aim of this research was to investigate the groundwater quality in El Barreal basin (Córdoba, Argentina), through bacteriological analysis and antibiotic resistance of fecal bacteria indicators and their relationship with geochemical and land uses characteristics. Groundwater samples were collected in 36 wells and the following parameters were determined: major chemical components, heterotrophic plate counts, total and fecal coliforms, Escherichia coli and Pseudomonas aeruginosa. Antibiotic resistance of E. coli was analyzed using standard methods. The chemical and bacteriological analysis showed that more than 80 % of samples were unfit for human consumption. Bacteriological contamination was significant in 36 % of samples. The multivariate analysis between bacterial and geochemical variables explained local contamination conditions, evidenced by the arrival of bacteria and some typical indicators (NO3-, Cl- y HCO3-) to groundwater. No significant correlation between NO3- and bacterial counts was observed, which let us to interpret that part of NO3- contents can be supplied by inorganic fertilizers. Total and fecal coliforms were linked to a local increase of Cl- and HCO3- and a decrease of pH and dissolved oxygen, indicating the arrival and degradation of organic matter into groundwater. The resistance pattern of total E. coli isolates (n=12) showed that the highest percentages were observed for antibiotics of animal use (ampicillin, tetracycline and cephalothin). The analysis of results revealed the impact of land uses demonstrating that livestock activities are the main punctual contaminant sources in this sedimentary aquifer.
Fil: Gambero, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina
Fil: Blarasin, Mónica Teresa. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Geología; Argentina
Fil: Bettera, Susana Gertrudis. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina
Fil: Matteoda, Edel Mara. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Geología; Argentina
Materia
ANTIBIOTICS
BACTERIAL CONTAMINATION
HIDROGEOCHEMISTRY
LAND USES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/74464
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Bacterial indicators and antibiotic resistance of Escherichia coli in groundwaterGambero, Maria LauraBlarasin, Mónica TeresaBettera, Susana GertrudisMatteoda, Edel MaraANTIBIOTICSBACTERIAL CONTAMINATIONHIDROGEOCHEMISTRYLAND USEShttps://purl.org/becyt/ford/1.5https://purl.org/becyt/ford/1The aim of this research was to investigate the groundwater quality in El Barreal basin (Córdoba, Argentina), through bacteriological analysis and antibiotic resistance of fecal bacteria indicators and their relationship with geochemical and land uses characteristics. Groundwater samples were collected in 36 wells and the following parameters were determined: major chemical components, heterotrophic plate counts, total and fecal coliforms, Escherichia coli and Pseudomonas aeruginosa. Antibiotic resistance of E. coli was analyzed using standard methods. The chemical and bacteriological analysis showed that more than 80 % of samples were unfit for human consumption. Bacteriological contamination was significant in 36 % of samples. The multivariate analysis between bacterial and geochemical variables explained local contamination conditions, evidenced by the arrival of bacteria and some typical indicators (NO3-, Cl- y HCO3-) to groundwater. No significant correlation between NO3- and bacterial counts was observed, which let us to interpret that part of NO3- contents can be supplied by inorganic fertilizers. Total and fecal coliforms were linked to a local increase of Cl- and HCO3- and a decrease of pH and dissolved oxygen, indicating the arrival and degradation of organic matter into groundwater. The resistance pattern of total E. coli isolates (n=12) showed that the highest percentages were observed for antibiotics of animal use (ampicillin, tetracycline and cephalothin). The analysis of results revealed the impact of land uses demonstrating that livestock activities are the main punctual contaminant sources in this sedimentary aquifer.Fil: Gambero, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; ArgentinaFil: Blarasin, Mónica Teresa. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Geología; ArgentinaFil: Bettera, Susana Gertrudis. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; ArgentinaFil: Matteoda, Edel Mara. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Geología; ArgentinaChief & Team2017-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/74464Gambero, Maria Laura; Blarasin, Mónica Teresa; Bettera, Susana Gertrudis; Matteoda, Edel Mara; Bacterial indicators and antibiotic resistance of Escherichia coli in groundwater; Chief & Team; International Journal of Environmental & Agriculture Research; 3; 11; 11-2017; 17-222454-1850CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.25125/agriculture-journal-IJOEAR-OCT-2017-21info:eu-repo/semantics/altIdentifier/url/http://ijoear.com/Paper-November-2017/IJOEAR-OCT-2017-21.pdfinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:57:14Zoai:ri.conicet.gov.ar:11336/74464instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:57:14.508CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Bacterial indicators and antibiotic resistance of Escherichia coli in groundwater
title Bacterial indicators and antibiotic resistance of Escherichia coli in groundwater
spellingShingle Bacterial indicators and antibiotic resistance of Escherichia coli in groundwater
Gambero, Maria Laura
ANTIBIOTICS
BACTERIAL CONTAMINATION
HIDROGEOCHEMISTRY
LAND USES
title_short Bacterial indicators and antibiotic resistance of Escherichia coli in groundwater
title_full Bacterial indicators and antibiotic resistance of Escherichia coli in groundwater
title_fullStr Bacterial indicators and antibiotic resistance of Escherichia coli in groundwater
title_full_unstemmed Bacterial indicators and antibiotic resistance of Escherichia coli in groundwater
title_sort Bacterial indicators and antibiotic resistance of Escherichia coli in groundwater
dc.creator.none.fl_str_mv Gambero, Maria Laura
Blarasin, Mónica Teresa
Bettera, Susana Gertrudis
Matteoda, Edel Mara
author Gambero, Maria Laura
author_facet Gambero, Maria Laura
Blarasin, Mónica Teresa
Bettera, Susana Gertrudis
Matteoda, Edel Mara
author_role author
author2 Blarasin, Mónica Teresa
Bettera, Susana Gertrudis
Matteoda, Edel Mara
author2_role author
author
author
dc.subject.none.fl_str_mv ANTIBIOTICS
BACTERIAL CONTAMINATION
HIDROGEOCHEMISTRY
LAND USES
topic ANTIBIOTICS
BACTERIAL CONTAMINATION
HIDROGEOCHEMISTRY
LAND USES
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.5
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The aim of this research was to investigate the groundwater quality in El Barreal basin (Córdoba, Argentina), through bacteriological analysis and antibiotic resistance of fecal bacteria indicators and their relationship with geochemical and land uses characteristics. Groundwater samples were collected in 36 wells and the following parameters were determined: major chemical components, heterotrophic plate counts, total and fecal coliforms, Escherichia coli and Pseudomonas aeruginosa. Antibiotic resistance of E. coli was analyzed using standard methods. The chemical and bacteriological analysis showed that more than 80 % of samples were unfit for human consumption. Bacteriological contamination was significant in 36 % of samples. The multivariate analysis between bacterial and geochemical variables explained local contamination conditions, evidenced by the arrival of bacteria and some typical indicators (NO3-, Cl- y HCO3-) to groundwater. No significant correlation between NO3- and bacterial counts was observed, which let us to interpret that part of NO3- contents can be supplied by inorganic fertilizers. Total and fecal coliforms were linked to a local increase of Cl- and HCO3- and a decrease of pH and dissolved oxygen, indicating the arrival and degradation of organic matter into groundwater. The resistance pattern of total E. coli isolates (n=12) showed that the highest percentages were observed for antibiotics of animal use (ampicillin, tetracycline and cephalothin). The analysis of results revealed the impact of land uses demonstrating that livestock activities are the main punctual contaminant sources in this sedimentary aquifer.
Fil: Gambero, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina
Fil: Blarasin, Mónica Teresa. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Geología; Argentina
Fil: Bettera, Susana Gertrudis. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina
Fil: Matteoda, Edel Mara. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Geología; Argentina
description The aim of this research was to investigate the groundwater quality in El Barreal basin (Córdoba, Argentina), through bacteriological analysis and antibiotic resistance of fecal bacteria indicators and their relationship with geochemical and land uses characteristics. Groundwater samples were collected in 36 wells and the following parameters were determined: major chemical components, heterotrophic plate counts, total and fecal coliforms, Escherichia coli and Pseudomonas aeruginosa. Antibiotic resistance of E. coli was analyzed using standard methods. The chemical and bacteriological analysis showed that more than 80 % of samples were unfit for human consumption. Bacteriological contamination was significant in 36 % of samples. The multivariate analysis between bacterial and geochemical variables explained local contamination conditions, evidenced by the arrival of bacteria and some typical indicators (NO3-, Cl- y HCO3-) to groundwater. No significant correlation between NO3- and bacterial counts was observed, which let us to interpret that part of NO3- contents can be supplied by inorganic fertilizers. Total and fecal coliforms were linked to a local increase of Cl- and HCO3- and a decrease of pH and dissolved oxygen, indicating the arrival and degradation of organic matter into groundwater. The resistance pattern of total E. coli isolates (n=12) showed that the highest percentages were observed for antibiotics of animal use (ampicillin, tetracycline and cephalothin). The analysis of results revealed the impact of land uses demonstrating that livestock activities are the main punctual contaminant sources in this sedimentary aquifer.
publishDate 2017
dc.date.none.fl_str_mv 2017-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/74464
Gambero, Maria Laura; Blarasin, Mónica Teresa; Bettera, Susana Gertrudis; Matteoda, Edel Mara; Bacterial indicators and antibiotic resistance of Escherichia coli in groundwater; Chief & Team; International Journal of Environmental & Agriculture Research; 3; 11; 11-2017; 17-22
2454-1850
CONICET Digital
CONICET
url http://hdl.handle.net/11336/74464
identifier_str_mv Gambero, Maria Laura; Blarasin, Mónica Teresa; Bettera, Susana Gertrudis; Matteoda, Edel Mara; Bacterial indicators and antibiotic resistance of Escherichia coli in groundwater; Chief & Team; International Journal of Environmental & Agriculture Research; 3; 11; 11-2017; 17-22
2454-1850
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.25125/agriculture-journal-IJOEAR-OCT-2017-21
info:eu-repo/semantics/altIdentifier/url/http://ijoear.com/Paper-November-2017/IJOEAR-OCT-2017-21.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Chief & Team
publisher.none.fl_str_mv Chief & Team
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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