Genetic diversity of Escherichia coli isolates from surface water and groundwater in a rural environment

Autores
Gambero, Maria Laura; Blarasin, Mónica Teresa; Bettera, Susana Gertrudis; Giuliano Albo, María Jesica
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The genetic characteristics among Escherichia coli strains can be grouped by origin of isolation. Then, it is possible to use the genotypes as a tool to determine the source of water contamination. The aim of this study was to define water aptitude for human consumption in a rural basin and to assess the diversity of E. coli water populations. Thus, it was possible to identify the main sources of fecal contamination and to explore linkages with the hydrogeological environment and land uses. The bacteriological analysis showed that more than 50% of samples were unfit for human consumption. DNA fingerprinting analysis by BOX-PCR indicated low genotypic diversity of E. coli isolates taken from surface water and groundwater. The results suggested the presence of a dominant source of fecal contamination. The relationship between low genotypic diversity and land use would prove that water contamination comes from livestock. The genetic diversity of E. coli isolated from surface water was less than that identified in groundwater because of the different hydraulic features of both environments. Furthermore, each one of the two big strain groups identified in this basin is located in different sub-basins, showing that hydrological dynamics exerts selective pressure on bacteria DNA.
Fil: Gambero, Maria Laura. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Blarasin, Mónica Teresa. Universidad Nacional de Rio Cuarto. Facultad de Cs.exactas Fisicoquimicas y Naturales. Departamento de Geologia. Cat.de Hidrogeologia; Argentina
Fil: Bettera, Susana Gertrudis. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina
Fil: Giuliano Albo, María Jesica. Universidad Nacional de Rio Cuarto. Facultad de Cs.exactas Fisicoquimicas y Naturales. Departamento de Geologia. Cat.de Hidrogeologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
E. COLI
GROUNDWATER
CONTAMINATION
RURAL ENVIRONMENT
BOC PCR
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/155451
Acceder
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Genetic diversity of Escherichia coli isolates from surface water and groundwater in a rural environmentGambero, Maria LauraBlarasin, Mónica TeresaBettera, Susana GertrudisGiuliano Albo, María JesicaE. COLIGROUNDWATERCONTAMINATIONRURAL ENVIRONMENTBOC PCRhttps://purl.org/becyt/ford/1.5https://purl.org/becyt/ford/1The genetic characteristics among Escherichia coli strains can be grouped by origin of isolation. Then, it is possible to use the genotypes as a tool to determine the source of water contamination. The aim of this study was to define water aptitude for human consumption in a rural basin and to assess the diversity of E. coli water populations. Thus, it was possible to identify the main sources of fecal contamination and to explore linkages with the hydrogeological environment and land uses. The bacteriological analysis showed that more than 50% of samples were unfit for human consumption. DNA fingerprinting analysis by BOX-PCR indicated low genotypic diversity of E. coli isolates taken from surface water and groundwater. The results suggested the presence of a dominant source of fecal contamination. The relationship between low genotypic diversity and land use would prove that water contamination comes from livestock. The genetic diversity of E. coli isolated from surface water was less than that identified in groundwater because of the different hydraulic features of both environments. Furthermore, each one of the two big strain groups identified in this basin is located in different sub-basins, showing that hydrological dynamics exerts selective pressure on bacteria DNA.Fil: Gambero, Maria Laura. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Blarasin, Mónica Teresa. Universidad Nacional de Rio Cuarto. Facultad de Cs.exactas Fisicoquimicas y Naturales. Departamento de Geologia. Cat.de Hidrogeologia; ArgentinaFil: Bettera, Susana Gertrudis. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; ArgentinaFil: Giuliano Albo, María Jesica. Universidad Nacional de Rio Cuarto. Facultad de Cs.exactas Fisicoquimicas y Naturales. Departamento de Geologia. Cat.de Hidrogeologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaIWA Publishing2017-08-18info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/155451Gambero, Maria Laura; Blarasin, Mónica Teresa; Bettera, Susana Gertrudis; Giuliano Albo, María Jesica; Genetic diversity of Escherichia coli isolates from surface water and groundwater in a rural environment; IWA Publishing; Journal of Water and Health; 15; 5; 18-8-2017; 757-7651477-89201996-7829CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://jwh.iwaponline.com/lookup/doi/10.2166/wh.2017.281info:eu-repo/semantics/altIdentifier/doi/10.2166/wh.2017.281info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:03:19Zoai:ri.conicet.gov.ar:11336/155451instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:03:19.847CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Genetic diversity of Escherichia coli isolates from surface water and groundwater in a rural environment
title Genetic diversity of Escherichia coli isolates from surface water and groundwater in a rural environment
spellingShingle Genetic diversity of Escherichia coli isolates from surface water and groundwater in a rural environment
Gambero, Maria Laura
E. COLI
GROUNDWATER
CONTAMINATION
RURAL ENVIRONMENT
BOC PCR
title_short Genetic diversity of Escherichia coli isolates from surface water and groundwater in a rural environment
title_full Genetic diversity of Escherichia coli isolates from surface water and groundwater in a rural environment
title_fullStr Genetic diversity of Escherichia coli isolates from surface water and groundwater in a rural environment
title_full_unstemmed Genetic diversity of Escherichia coli isolates from surface water and groundwater in a rural environment
title_sort Genetic diversity of Escherichia coli isolates from surface water and groundwater in a rural environment
dc.creator.none.fl_str_mv Gambero, Maria Laura
Blarasin, Mónica Teresa
Bettera, Susana Gertrudis
Giuliano Albo, María Jesica
author Gambero, Maria Laura
author_facet Gambero, Maria Laura
Blarasin, Mónica Teresa
Bettera, Susana Gertrudis
Giuliano Albo, María Jesica
author_role author
author2 Blarasin, Mónica Teresa
Bettera, Susana Gertrudis
Giuliano Albo, María Jesica
author2_role author
author
author
dc.subject.none.fl_str_mv E. COLI
GROUNDWATER
CONTAMINATION
RURAL ENVIRONMENT
BOC PCR
topic E. COLI
GROUNDWATER
CONTAMINATION
RURAL ENVIRONMENT
BOC PCR
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.5
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The genetic characteristics among Escherichia coli strains can be grouped by origin of isolation. Then, it is possible to use the genotypes as a tool to determine the source of water contamination. The aim of this study was to define water aptitude for human consumption in a rural basin and to assess the diversity of E. coli water populations. Thus, it was possible to identify the main sources of fecal contamination and to explore linkages with the hydrogeological environment and land uses. The bacteriological analysis showed that more than 50% of samples were unfit for human consumption. DNA fingerprinting analysis by BOX-PCR indicated low genotypic diversity of E. coli isolates taken from surface water and groundwater. The results suggested the presence of a dominant source of fecal contamination. The relationship between low genotypic diversity and land use would prove that water contamination comes from livestock. The genetic diversity of E. coli isolated from surface water was less than that identified in groundwater because of the different hydraulic features of both environments. Furthermore, each one of the two big strain groups identified in this basin is located in different sub-basins, showing that hydrological dynamics exerts selective pressure on bacteria DNA.
Fil: Gambero, Maria Laura. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Blarasin, Mónica Teresa. Universidad Nacional de Rio Cuarto. Facultad de Cs.exactas Fisicoquimicas y Naturales. Departamento de Geologia. Cat.de Hidrogeologia; Argentina
Fil: Bettera, Susana Gertrudis. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina
Fil: Giuliano Albo, María Jesica. Universidad Nacional de Rio Cuarto. Facultad de Cs.exactas Fisicoquimicas y Naturales. Departamento de Geologia. Cat.de Hidrogeologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description The genetic characteristics among Escherichia coli strains can be grouped by origin of isolation. Then, it is possible to use the genotypes as a tool to determine the source of water contamination. The aim of this study was to define water aptitude for human consumption in a rural basin and to assess the diversity of E. coli water populations. Thus, it was possible to identify the main sources of fecal contamination and to explore linkages with the hydrogeological environment and land uses. The bacteriological analysis showed that more than 50% of samples were unfit for human consumption. DNA fingerprinting analysis by BOX-PCR indicated low genotypic diversity of E. coli isolates taken from surface water and groundwater. The results suggested the presence of a dominant source of fecal contamination. The relationship between low genotypic diversity and land use would prove that water contamination comes from livestock. The genetic diversity of E. coli isolated from surface water was less than that identified in groundwater because of the different hydraulic features of both environments. Furthermore, each one of the two big strain groups identified in this basin is located in different sub-basins, showing that hydrological dynamics exerts selective pressure on bacteria DNA.
publishDate 2017
dc.date.none.fl_str_mv 2017-08-18
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/155451
Gambero, Maria Laura; Blarasin, Mónica Teresa; Bettera, Susana Gertrudis; Giuliano Albo, María Jesica; Genetic diversity of Escherichia coli isolates from surface water and groundwater in a rural environment; IWA Publishing; Journal of Water and Health; 15; 5; 18-8-2017; 757-765
1477-8920
1996-7829
CONICET Digital
CONICET
url http://hdl.handle.net/11336/155451
identifier_str_mv Gambero, Maria Laura; Blarasin, Mónica Teresa; Bettera, Susana Gertrudis; Giuliano Albo, María Jesica; Genetic diversity of Escherichia coli isolates from surface water and groundwater in a rural environment; IWA Publishing; Journal of Water and Health; 15; 5; 18-8-2017; 757-765
1477-8920
1996-7829
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://jwh.iwaponline.com/lookup/doi/10.2166/wh.2017.281
info:eu-repo/semantics/altIdentifier/doi/10.2166/wh.2017.281
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv IWA Publishing
publisher.none.fl_str_mv IWA Publishing
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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