Standardized Flow Cytometry Assay for Identification of Human Monocytic Heterogeneity and LRP1 Expression in Monocyte Subpopulations: Decreased Expression of This Receptor in Noncl...

Autores
Ferrer, Dario German; Jaldín Fincati, Javier Roberto; Amigone, Jose L.; Capra, Raul H.; Collino, Cesar J.; Albertini, Ricardo Arturo; Chiabrando, Gustavo Alberto
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In this article, we present a flow cytometry assay by which human blood monocyte sub-populations—classical (CD1411CD162), intermediate (CD1411CD161), and non-classical (CD141CD1611) monocytes—can be determined. Monocytic cells wereselected from CD451leukocyte subsets by differential staining of the low-density lipo-protein receptor-related protein 1 (LRP1), which allows reducing the spill-over of natu-ral killer cells and granulocytes into the CD161monocyte gate. Percentages ofmonocyte subpopulations established by this procedure were significantly comparablewith those obtained by a well-standardized flow cytometry assay based on the HLA-DRmonocyte-gating strategy. We also demonstrated that LRP1 is differentially expressed atcell surface of monocyte subpopulations, being significantly lower in nonclassicalmonocytes than in classical and intermediate monocytes. Cell surface expression ofLRP1 accounts for only 20% of the total cellular content in each monocyte subpopula-tion. Finally, we established the within-individual biological variation (bCV%) of cir-culating monocyte subpopulations in healthy donors, obtaining values of 21%, 20%,and 17% for nonclassical, intermediate, and classical monocytes, respectively. Similarvalues of bCV% for LRP1 measured in each monocyte subpopulation were alsoobtained, suggesting that its variability is mainly influenced by the intrinsic biologicalvariation of circulating monocytes. Thus, we conclude that LRP1 can be used as a thirdpan-monocytic marker together with CD14 and CD16 to properly identify monocytesubpopulations. The combined determination of monocyte subpopulations and LRP1monocytic expression may be relevant for clinical studies of inflammatory processes,with special interest in atherosclerosis and cardiovascular disease.VC2014 InternationalSociety for Advancement of Cytometry
Fil: Ferrer, Dario German. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Jaldín Fincati, Javier Roberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Amigone, Jose L.. Hospital Privado Centro Medico de Córdoba; Argentina
Fil: Capra, Raul H.. Hospital Privado Centro Medico de Córdoba; Argentina
Fil: Collino, Cesar J.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Albertini, Ricardo Arturo. Hospital Privado Centro Medico de Córdoba; Argentina
Fil: Chiabrando, Gustavo Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Materia
Monocyte Heterogeneity
Inflammation
Biological Variation
Atherosclerosis
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/31873
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/31873
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Standardized Flow Cytometry Assay for Identification of Human Monocytic Heterogeneity and LRP1 Expression in Monocyte Subpopulations: Decreased Expression of This Receptor in Nonclassical MonocytesFerrer, Dario GermanJaldín Fincati, Javier RobertoAmigone, Jose L.Capra, Raul H.Collino, Cesar J.Albertini, Ricardo ArturoChiabrando, Gustavo AlbertoMonocyte HeterogeneityInflammationBiological VariationAtherosclerosishttps://purl.org/becyt/ford/3.2https://purl.org/becyt/ford/3In this article, we present a flow cytometry assay by which human blood monocyte sub-populations—classical (CD1411CD162), intermediate (CD1411CD161), and non-classical (CD141CD1611) monocytes—can be determined. Monocytic cells wereselected from CD451leukocyte subsets by differential staining of the low-density lipo-protein receptor-related protein 1 (LRP1), which allows reducing the spill-over of natu-ral killer cells and granulocytes into the CD161monocyte gate. Percentages ofmonocyte subpopulations established by this procedure were significantly comparablewith those obtained by a well-standardized flow cytometry assay based on the HLA-DRmonocyte-gating strategy. We also demonstrated that LRP1 is differentially expressed atcell surface of monocyte subpopulations, being significantly lower in nonclassicalmonocytes than in classical and intermediate monocytes. Cell surface expression ofLRP1 accounts for only 20% of the total cellular content in each monocyte subpopula-tion. Finally, we established the within-individual biological variation (bCV%) of cir-culating monocyte subpopulations in healthy donors, obtaining values of 21%, 20%,and 17% for nonclassical, intermediate, and classical monocytes, respectively. Similarvalues of bCV% for LRP1 measured in each monocyte subpopulation were alsoobtained, suggesting that its variability is mainly influenced by the intrinsic biologicalvariation of circulating monocytes. Thus, we conclude that LRP1 can be used as a thirdpan-monocytic marker together with CD14 and CD16 to properly identify monocytesubpopulations. The combined determination of monocyte subpopulations and LRP1monocytic expression may be relevant for clinical studies of inflammatory processes,with special interest in atherosclerosis and cardiovascular disease.VC2014 InternationalSociety for Advancement of CytometryFil: Ferrer, Dario German. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaFil: Jaldín Fincati, Javier Roberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaFil: Amigone, Jose L.. Hospital Privado Centro Medico de Córdoba; ArgentinaFil: Capra, Raul H.. Hospital Privado Centro Medico de Córdoba; ArgentinaFil: Collino, Cesar J.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaFil: Albertini, Ricardo Arturo. Hospital Privado Centro Medico de Córdoba; ArgentinaFil: Chiabrando, Gustavo Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaWiley-liss, Div John Wiley & Sons Inc2014-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/31873Chiabrando, Gustavo Alberto; Albertini, Ricardo Arturo; Collino, Cesar J.; Capra, Raul H.; Amigone, Jose L.; Jaldín Fincati, Javier Roberto; et al.; Standardized Flow Cytometry Assay for Identification of Human Monocytic Heterogeneity and LRP1 Expression in Monocyte Subpopulations: Decreased Expression of This Receptor in Nonclassical Monocytes; Wiley-liss, Div John Wiley & Sons Inc; Cytometry Part A; 85; 7; 7-2014; 601-6101552-4922CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/cyto.a.22455/abstractinfo:eu-repo/semantics/altIdentifier/doi/10.1002/cyto.a.22455info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:24:55Zoai:ri.conicet.gov.ar:11336/31873instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:24:55.417CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Standardized Flow Cytometry Assay for Identification of Human Monocytic Heterogeneity and LRP1 Expression in Monocyte Subpopulations: Decreased Expression of This Receptor in Nonclassical Monocytes
title Standardized Flow Cytometry Assay for Identification of Human Monocytic Heterogeneity and LRP1 Expression in Monocyte Subpopulations: Decreased Expression of This Receptor in Nonclassical Monocytes
spellingShingle Standardized Flow Cytometry Assay for Identification of Human Monocytic Heterogeneity and LRP1 Expression in Monocyte Subpopulations: Decreased Expression of This Receptor in Nonclassical Monocytes
Ferrer, Dario German
Monocyte Heterogeneity
Inflammation
Biological Variation
Atherosclerosis
title_short Standardized Flow Cytometry Assay for Identification of Human Monocytic Heterogeneity and LRP1 Expression in Monocyte Subpopulations: Decreased Expression of This Receptor in Nonclassical Monocytes
title_full Standardized Flow Cytometry Assay for Identification of Human Monocytic Heterogeneity and LRP1 Expression in Monocyte Subpopulations: Decreased Expression of This Receptor in Nonclassical Monocytes
title_fullStr Standardized Flow Cytometry Assay for Identification of Human Monocytic Heterogeneity and LRP1 Expression in Monocyte Subpopulations: Decreased Expression of This Receptor in Nonclassical Monocytes
title_full_unstemmed Standardized Flow Cytometry Assay for Identification of Human Monocytic Heterogeneity and LRP1 Expression in Monocyte Subpopulations: Decreased Expression of This Receptor in Nonclassical Monocytes
title_sort Standardized Flow Cytometry Assay for Identification of Human Monocytic Heterogeneity and LRP1 Expression in Monocyte Subpopulations: Decreased Expression of This Receptor in Nonclassical Monocytes
dc.creator.none.fl_str_mv Ferrer, Dario German
Jaldín Fincati, Javier Roberto
Amigone, Jose L.
Capra, Raul H.
Collino, Cesar J.
Albertini, Ricardo Arturo
Chiabrando, Gustavo Alberto
author Ferrer, Dario German
author_facet Ferrer, Dario German
Jaldín Fincati, Javier Roberto
Amigone, Jose L.
Capra, Raul H.
Collino, Cesar J.
Albertini, Ricardo Arturo
Chiabrando, Gustavo Alberto
author_role author
author2 Jaldín Fincati, Javier Roberto
Amigone, Jose L.
Capra, Raul H.
Collino, Cesar J.
Albertini, Ricardo Arturo
Chiabrando, Gustavo Alberto
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Monocyte Heterogeneity
Inflammation
Biological Variation
Atherosclerosis
topic Monocyte Heterogeneity
Inflammation
Biological Variation
Atherosclerosis
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.2
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv In this article, we present a flow cytometry assay by which human blood monocyte sub-populations—classical (CD1411CD162), intermediate (CD1411CD161), and non-classical (CD141CD1611) monocytes—can be determined. Monocytic cells wereselected from CD451leukocyte subsets by differential staining of the low-density lipo-protein receptor-related protein 1 (LRP1), which allows reducing the spill-over of natu-ral killer cells and granulocytes into the CD161monocyte gate. Percentages ofmonocyte subpopulations established by this procedure were significantly comparablewith those obtained by a well-standardized flow cytometry assay based on the HLA-DRmonocyte-gating strategy. We also demonstrated that LRP1 is differentially expressed atcell surface of monocyte subpopulations, being significantly lower in nonclassicalmonocytes than in classical and intermediate monocytes. Cell surface expression ofLRP1 accounts for only 20% of the total cellular content in each monocyte subpopula-tion. Finally, we established the within-individual biological variation (bCV%) of cir-culating monocyte subpopulations in healthy donors, obtaining values of 21%, 20%,and 17% for nonclassical, intermediate, and classical monocytes, respectively. Similarvalues of bCV% for LRP1 measured in each monocyte subpopulation were alsoobtained, suggesting that its variability is mainly influenced by the intrinsic biologicalvariation of circulating monocytes. Thus, we conclude that LRP1 can be used as a thirdpan-monocytic marker together with CD14 and CD16 to properly identify monocytesubpopulations. The combined determination of monocyte subpopulations and LRP1monocytic expression may be relevant for clinical studies of inflammatory processes,with special interest in atherosclerosis and cardiovascular disease.VC2014 InternationalSociety for Advancement of Cytometry
Fil: Ferrer, Dario German. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Jaldín Fincati, Javier Roberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Amigone, Jose L.. Hospital Privado Centro Medico de Córdoba; Argentina
Fil: Capra, Raul H.. Hospital Privado Centro Medico de Córdoba; Argentina
Fil: Collino, Cesar J.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Albertini, Ricardo Arturo. Hospital Privado Centro Medico de Córdoba; Argentina
Fil: Chiabrando, Gustavo Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
description In this article, we present a flow cytometry assay by which human blood monocyte sub-populations—classical (CD1411CD162), intermediate (CD1411CD161), and non-classical (CD141CD1611) monocytes—can be determined. Monocytic cells wereselected from CD451leukocyte subsets by differential staining of the low-density lipo-protein receptor-related protein 1 (LRP1), which allows reducing the spill-over of natu-ral killer cells and granulocytes into the CD161monocyte gate. Percentages ofmonocyte subpopulations established by this procedure were significantly comparablewith those obtained by a well-standardized flow cytometry assay based on the HLA-DRmonocyte-gating strategy. We also demonstrated that LRP1 is differentially expressed atcell surface of monocyte subpopulations, being significantly lower in nonclassicalmonocytes than in classical and intermediate monocytes. Cell surface expression ofLRP1 accounts for only 20% of the total cellular content in each monocyte subpopula-tion. Finally, we established the within-individual biological variation (bCV%) of cir-culating monocyte subpopulations in healthy donors, obtaining values of 21%, 20%,and 17% for nonclassical, intermediate, and classical monocytes, respectively. Similarvalues of bCV% for LRP1 measured in each monocyte subpopulation were alsoobtained, suggesting that its variability is mainly influenced by the intrinsic biologicalvariation of circulating monocytes. Thus, we conclude that LRP1 can be used as a thirdpan-monocytic marker together with CD14 and CD16 to properly identify monocytesubpopulations. The combined determination of monocyte subpopulations and LRP1monocytic expression may be relevant for clinical studies of inflammatory processes,with special interest in atherosclerosis and cardiovascular disease.VC2014 InternationalSociety for Advancement of Cytometry
publishDate 2014
dc.date.none.fl_str_mv 2014-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/31873
Chiabrando, Gustavo Alberto; Albertini, Ricardo Arturo; Collino, Cesar J.; Capra, Raul H.; Amigone, Jose L.; Jaldín Fincati, Javier Roberto; et al.; Standardized Flow Cytometry Assay for Identification of Human Monocytic Heterogeneity and LRP1 Expression in Monocyte Subpopulations: Decreased Expression of This Receptor in Nonclassical Monocytes; Wiley-liss, Div John Wiley & Sons Inc; Cytometry Part A; 85; 7; 7-2014; 601-610
1552-4922
CONICET Digital
CONICET
url http://hdl.handle.net/11336/31873
identifier_str_mv Chiabrando, Gustavo Alberto; Albertini, Ricardo Arturo; Collino, Cesar J.; Capra, Raul H.; Amigone, Jose L.; Jaldín Fincati, Javier Roberto; et al.; Standardized Flow Cytometry Assay for Identification of Human Monocytic Heterogeneity and LRP1 Expression in Monocyte Subpopulations: Decreased Expression of This Receptor in Nonclassical Monocytes; Wiley-liss, Div John Wiley & Sons Inc; Cytometry Part A; 85; 7; 7-2014; 601-610
1552-4922
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/cyto.a.22455/abstract
info:eu-repo/semantics/altIdentifier/doi/10.1002/cyto.a.22455
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley-liss, Div John Wiley & Sons Inc
publisher.none.fl_str_mv Wiley-liss, Div John Wiley & Sons Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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