Chemometric resolution of fully overlapped capillary electrophoresis peaks: quantitation of carbamazepine in human serum in the presence of several interferences

Autores
Vera Candioti, Luciana; Culzoni, Maria Julia; Olivieri, Alejandro Cesar; Goicoechea, Hector Casimiro
Año de publicación
2008
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Drug monitoring in serum samples was performed using second-order data generated by CE-DAD, processed with a suitable chemometric strategy. Carbamazepine could be accurately quantitated in the presence of its main metabolite (carbamazepine epoxide),other therapeutic drugs (lamotrigine, phenobarbital, phenytoin, phenylephrine,ibuprofen, acetaminophen, theophylline, caffeine, acetyl salicylic acid), and additional
serum endogenous components. The analytical strategy consisted of the following steps:(i) serum sample clean-up to remove matrix interferences, (ii) data pre-processing,in order to reduce the background and to correct for electrophoretic time shifts, and (iii) resolution of fully overlapped CE peaks (corresponding to carbamazepine, its metabolite, lamotrigine and unexpected serum components) by the well-known multivariate curve resolution-alternating least squares algorithm, which extracts quantitative information that can be uniquely ascribed to the analyte of interest. The analyte concentration in serum samples ranged from 2.00 to 8.00 mg/L. Mean recoveries
were 102.6% (s57.7) for binary samples, and 94.8% (s513.5) for spiked serum samples,while CV (%)54.0 was computed for five replicate, indicative of the acceptable accuracy and precision of the proposed method
Fil: Vera Candioti, Luciana. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Química. Cátedra de Química Analítica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Culzoni, Maria Julia. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Química. Cátedra de Química Analítica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Olivieri, Alejandro Cesar. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Departamento de Química Analítica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Goicoechea, Hector Casimiro. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Química. Cátedra de Química Analítica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
Carbamazepine
Overlapped Peaks
Second-Order Advantage
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/25385
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/25385
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Chemometric resolution of fully overlapped capillary electrophoresis peaks: quantitation of carbamazepine in human serum in the presence of several interferencesVera Candioti, LucianaCulzoni, Maria JuliaOlivieri, Alejandro CesarGoicoechea, Hector CasimiroCarbamazepineOverlapped PeaksSecond-Order Advantagehttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Drug monitoring in serum samples was performed using second-order data generated by CE-DAD, processed with a suitable chemometric strategy. Carbamazepine could be accurately quantitated in the presence of its main metabolite (carbamazepine epoxide),other therapeutic drugs (lamotrigine, phenobarbital, phenytoin, phenylephrine,ibuprofen, acetaminophen, theophylline, caffeine, acetyl salicylic acid), and additional<br />serum endogenous components. The analytical strategy consisted of the following steps:(i) serum sample clean-up to remove matrix interferences, (ii) data pre-processing,in order to reduce the background and to correct for electrophoretic time shifts, and (iii) resolution of fully overlapped CE peaks (corresponding to carbamazepine, its metabolite, lamotrigine and unexpected serum components) by the well-known multivariate curve resolution-alternating least squares algorithm, which extracts quantitative information that can be uniquely ascribed to the analyte of interest. The analyte concentration in serum samples ranged from 2.00 to 8.00 mg/L. Mean recoveries<br />were 102.6% (s57.7) for binary samples, and 94.8% (s513.5) for spiked serum samples,while CV (%)54.0 was computed for five replicate, indicative of the acceptable accuracy and precision of the proposed methodFil: Vera Candioti, Luciana. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Química. Cátedra de Química Analítica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Culzoni, Maria Julia. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Química. Cátedra de Química Analítica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Olivieri, Alejandro Cesar. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Departamento de Química Analítica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Goicoechea, Hector Casimiro. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Química. Cátedra de Química Analítica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaWiley VCH Verlag2008-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/25385Vera Candioti, Luciana; Culzoni, Maria Julia; Olivieri, Alejandro Cesar; Goicoechea, Hector Casimiro; Chemometric resolution of fully overlapped capillary electrophoresis peaks: quantitation of carbamazepine in human serum in the presence of several interferences; Wiley VCH Verlag; Electrophoresis; 29; 22; 12-2008; 4527-45370173-0835CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1002/elps.200800400info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/elps.200800400/abstractinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:43:42Zoai:ri.conicet.gov.ar:11336/25385instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:43:42.492CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Chemometric resolution of fully overlapped capillary electrophoresis peaks: quantitation of carbamazepine in human serum in the presence of several interferences
title Chemometric resolution of fully overlapped capillary electrophoresis peaks: quantitation of carbamazepine in human serum in the presence of several interferences
spellingShingle Chemometric resolution of fully overlapped capillary electrophoresis peaks: quantitation of carbamazepine in human serum in the presence of several interferences
Vera Candioti, Luciana
Carbamazepine
Overlapped Peaks
Second-Order Advantage
title_short Chemometric resolution of fully overlapped capillary electrophoresis peaks: quantitation of carbamazepine in human serum in the presence of several interferences
title_full Chemometric resolution of fully overlapped capillary electrophoresis peaks: quantitation of carbamazepine in human serum in the presence of several interferences
title_fullStr Chemometric resolution of fully overlapped capillary electrophoresis peaks: quantitation of carbamazepine in human serum in the presence of several interferences
title_full_unstemmed Chemometric resolution of fully overlapped capillary electrophoresis peaks: quantitation of carbamazepine in human serum in the presence of several interferences
title_sort Chemometric resolution of fully overlapped capillary electrophoresis peaks: quantitation of carbamazepine in human serum in the presence of several interferences
dc.creator.none.fl_str_mv Vera Candioti, Luciana
Culzoni, Maria Julia
Olivieri, Alejandro Cesar
Goicoechea, Hector Casimiro
author Vera Candioti, Luciana
author_facet Vera Candioti, Luciana
Culzoni, Maria Julia
Olivieri, Alejandro Cesar
Goicoechea, Hector Casimiro
author_role author
author2 Culzoni, Maria Julia
Olivieri, Alejandro Cesar
Goicoechea, Hector Casimiro
author2_role author
author
author
dc.subject.none.fl_str_mv Carbamazepine
Overlapped Peaks
Second-Order Advantage
topic Carbamazepine
Overlapped Peaks
Second-Order Advantage
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Drug monitoring in serum samples was performed using second-order data generated by CE-DAD, processed with a suitable chemometric strategy. Carbamazepine could be accurately quantitated in the presence of its main metabolite (carbamazepine epoxide),other therapeutic drugs (lamotrigine, phenobarbital, phenytoin, phenylephrine,ibuprofen, acetaminophen, theophylline, caffeine, acetyl salicylic acid), and additional<br />serum endogenous components. The analytical strategy consisted of the following steps:(i) serum sample clean-up to remove matrix interferences, (ii) data pre-processing,in order to reduce the background and to correct for electrophoretic time shifts, and (iii) resolution of fully overlapped CE peaks (corresponding to carbamazepine, its metabolite, lamotrigine and unexpected serum components) by the well-known multivariate curve resolution-alternating least squares algorithm, which extracts quantitative information that can be uniquely ascribed to the analyte of interest. The analyte concentration in serum samples ranged from 2.00 to 8.00 mg/L. Mean recoveries<br />were 102.6% (s57.7) for binary samples, and 94.8% (s513.5) for spiked serum samples,while CV (%)54.0 was computed for five replicate, indicative of the acceptable accuracy and precision of the proposed method
Fil: Vera Candioti, Luciana. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Química. Cátedra de Química Analítica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Culzoni, Maria Julia. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Química. Cátedra de Química Analítica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Olivieri, Alejandro Cesar. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Departamento de Química Analítica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Goicoechea, Hector Casimiro. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Química. Cátedra de Química Analítica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Drug monitoring in serum samples was performed using second-order data generated by CE-DAD, processed with a suitable chemometric strategy. Carbamazepine could be accurately quantitated in the presence of its main metabolite (carbamazepine epoxide),other therapeutic drugs (lamotrigine, phenobarbital, phenytoin, phenylephrine,ibuprofen, acetaminophen, theophylline, caffeine, acetyl salicylic acid), and additional<br />serum endogenous components. The analytical strategy consisted of the following steps:(i) serum sample clean-up to remove matrix interferences, (ii) data pre-processing,in order to reduce the background and to correct for electrophoretic time shifts, and (iii) resolution of fully overlapped CE peaks (corresponding to carbamazepine, its metabolite, lamotrigine and unexpected serum components) by the well-known multivariate curve resolution-alternating least squares algorithm, which extracts quantitative information that can be uniquely ascribed to the analyte of interest. The analyte concentration in serum samples ranged from 2.00 to 8.00 mg/L. Mean recoveries<br />were 102.6% (s57.7) for binary samples, and 94.8% (s513.5) for spiked serum samples,while CV (%)54.0 was computed for five replicate, indicative of the acceptable accuracy and precision of the proposed method
publishDate 2008
dc.date.none.fl_str_mv 2008-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/25385
Vera Candioti, Luciana; Culzoni, Maria Julia; Olivieri, Alejandro Cesar; Goicoechea, Hector Casimiro; Chemometric resolution of fully overlapped capillary electrophoresis peaks: quantitation of carbamazepine in human serum in the presence of several interferences; Wiley VCH Verlag; Electrophoresis; 29; 22; 12-2008; 4527-4537
0173-0835
CONICET Digital
CONICET
url http://hdl.handle.net/11336/25385
identifier_str_mv Vera Candioti, Luciana; Culzoni, Maria Julia; Olivieri, Alejandro Cesar; Goicoechea, Hector Casimiro; Chemometric resolution of fully overlapped capillary electrophoresis peaks: quantitation of carbamazepine in human serum in the presence of several interferences; Wiley VCH Verlag; Electrophoresis; 29; 22; 12-2008; 4527-4537
0173-0835
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1002/elps.200800400
info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/elps.200800400/abstract
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley VCH Verlag
publisher.none.fl_str_mv Wiley VCH Verlag
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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