Immunogenicity of a Bovine Herpes Virus I Peptide Expressed in Tandem Copies in Attenuated Salmonella

Autores
Gnazzo, Victoria; Cebrián, José Ignacio; Langellotti, Cecilia Ana; Chabalgoity, José; Mongini, Claudia; Quattrocchi, Valeria; Zamorano, Patricia Ines
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A live system to release heterologous antigens using an attenuated Salmonella strain was developed. We transformed Salmonella typhimurium LVR03 (S. LVR03) with a recombinant pTECH2 vector encoding 0, 1, 2, and 4 tandem copies of an imunogenic peptide of bovine herpes virus-1 (BoHV-1) glycoprotein D (gD). The system used yielded peptides fused to the non-toxic C fragment of the tetanus toxin (TetC), which has been shown to have adjuvant properties. Inoculation of BALB/c mice with the transformed Salmonella strains gave rise to a mild self-limited infection, with primary replication of bacteria occurring in Peyer´s patches, even when the bacteria was administered intranasally. Humoral and cellular immune responses directed against the BoHV-1 antigens were evaluated after oral or intranasal administration of the recombinant bacteria. The results showed that the S. LVR03-dimer vaccine induced specific humoral (IgG in serum and IgG(1) and IgA in saliva), and cellular immune responses (lymphoproliferation and lymphokine secretion), against not only the selected peptide and whole gD, but also against BoHV-1, when administered intranasally. This is the first time Salmonella has been used as an expression vector to induce immunity against BoHV-1. This work demonstrates the feasibility of using this antigen-release system and encourages future experimentation with a bovine experimental model.
Fil: Gnazzo, Victoria. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cebrián, José Ignacio. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Langellotti, Cecilia Ana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Chabalgoity, José. Universidad de Concepción del Uruguay; Argentina
Fil: Mongini, Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Quattrocchi, Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina
Fil: Zamorano, Patricia Ines. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
ATTENUATED SALMONELLA
BOVINE HERPES VIRUS I
VACCINE
BACTERIAL VECTORS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/269637
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/269637
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Immunogenicity of a Bovine Herpes Virus I Peptide Expressed in Tandem Copies in Attenuated SalmonellaGnazzo, VictoriaCebrián, José IgnacioLangellotti, Cecilia AnaChabalgoity, JoséMongini, ClaudiaQuattrocchi, ValeriaZamorano, Patricia InesATTENUATED SALMONELLABOVINE HERPES VIRUS IVACCINEBACTERIAL VECTORShttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4A live system to release heterologous antigens using an attenuated Salmonella strain was developed. We transformed Salmonella typhimurium LVR03 (S. LVR03) with a recombinant pTECH2 vector encoding 0, 1, 2, and 4 tandem copies of an imunogenic peptide of bovine herpes virus-1 (BoHV-1) glycoprotein D (gD). The system used yielded peptides fused to the non-toxic C fragment of the tetanus toxin (TetC), which has been shown to have adjuvant properties. Inoculation of BALB/c mice with the transformed Salmonella strains gave rise to a mild self-limited infection, with primary replication of bacteria occurring in Peyer´s patches, even when the bacteria was administered intranasally. Humoral and cellular immune responses directed against the BoHV-1 antigens were evaluated after oral or intranasal administration of the recombinant bacteria. The results showed that the S. LVR03-dimer vaccine induced specific humoral (IgG in serum and IgG(1) and IgA in saliva), and cellular immune responses (lymphoproliferation and lymphokine secretion), against not only the selected peptide and whole gD, but also against BoHV-1, when administered intranasally. This is the first time Salmonella has been used as an expression vector to induce immunity against BoHV-1. This work demonstrates the feasibility of using this antigen-release system and encourages future experimentation with a bovine experimental model.Fil: Gnazzo, Victoria. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Cebrián, José Ignacio. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Langellotti, Cecilia Ana. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Chabalgoity, José. Universidad de Concepción del Uruguay; ArgentinaFil: Mongini, Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Quattrocchi, Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; ArgentinaFil: Zamorano, Patricia Ines. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaMary Ann Liebert2012-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/269637Gnazzo, Victoria; Cebrián, José Ignacio; Langellotti, Cecilia Ana; Chabalgoity, José; Mongini, Claudia; et al.; Immunogenicity of a Bovine Herpes Virus I Peptide Expressed in Tandem Copies in Attenuated Salmonella; Mary Ann Liebert; Viral Immunology; 25; 1; 2-2012; 63-720882-8245CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.liebertpub.com/doi/10.1089/vim.2011.0031info:eu-repo/semantics/altIdentifier/doi/10.1089/vim.2011.0031info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:48:39Zoai:ri.conicet.gov.ar:11336/269637instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:48:40.372CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Immunogenicity of a Bovine Herpes Virus I Peptide Expressed in Tandem Copies in Attenuated Salmonella
title Immunogenicity of a Bovine Herpes Virus I Peptide Expressed in Tandem Copies in Attenuated Salmonella
spellingShingle Immunogenicity of a Bovine Herpes Virus I Peptide Expressed in Tandem Copies in Attenuated Salmonella
Gnazzo, Victoria
ATTENUATED SALMONELLA
BOVINE HERPES VIRUS I
VACCINE
BACTERIAL VECTORS
title_short Immunogenicity of a Bovine Herpes Virus I Peptide Expressed in Tandem Copies in Attenuated Salmonella
title_full Immunogenicity of a Bovine Herpes Virus I Peptide Expressed in Tandem Copies in Attenuated Salmonella
title_fullStr Immunogenicity of a Bovine Herpes Virus I Peptide Expressed in Tandem Copies in Attenuated Salmonella
title_full_unstemmed Immunogenicity of a Bovine Herpes Virus I Peptide Expressed in Tandem Copies in Attenuated Salmonella
title_sort Immunogenicity of a Bovine Herpes Virus I Peptide Expressed in Tandem Copies in Attenuated Salmonella
dc.creator.none.fl_str_mv Gnazzo, Victoria
Cebrián, José Ignacio
Langellotti, Cecilia Ana
Chabalgoity, José
Mongini, Claudia
Quattrocchi, Valeria
Zamorano, Patricia Ines
author Gnazzo, Victoria
author_facet Gnazzo, Victoria
Cebrián, José Ignacio
Langellotti, Cecilia Ana
Chabalgoity, José
Mongini, Claudia
Quattrocchi, Valeria
Zamorano, Patricia Ines
author_role author
author2 Cebrián, José Ignacio
Langellotti, Cecilia Ana
Chabalgoity, José
Mongini, Claudia
Quattrocchi, Valeria
Zamorano, Patricia Ines
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv ATTENUATED SALMONELLA
BOVINE HERPES VIRUS I
VACCINE
BACTERIAL VECTORS
topic ATTENUATED SALMONELLA
BOVINE HERPES VIRUS I
VACCINE
BACTERIAL VECTORS
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv A live system to release heterologous antigens using an attenuated Salmonella strain was developed. We transformed Salmonella typhimurium LVR03 (S. LVR03) with a recombinant pTECH2 vector encoding 0, 1, 2, and 4 tandem copies of an imunogenic peptide of bovine herpes virus-1 (BoHV-1) glycoprotein D (gD). The system used yielded peptides fused to the non-toxic C fragment of the tetanus toxin (TetC), which has been shown to have adjuvant properties. Inoculation of BALB/c mice with the transformed Salmonella strains gave rise to a mild self-limited infection, with primary replication of bacteria occurring in Peyer´s patches, even when the bacteria was administered intranasally. Humoral and cellular immune responses directed against the BoHV-1 antigens were evaluated after oral or intranasal administration of the recombinant bacteria. The results showed that the S. LVR03-dimer vaccine induced specific humoral (IgG in serum and IgG(1) and IgA in saliva), and cellular immune responses (lymphoproliferation and lymphokine secretion), against not only the selected peptide and whole gD, but also against BoHV-1, when administered intranasally. This is the first time Salmonella has been used as an expression vector to induce immunity against BoHV-1. This work demonstrates the feasibility of using this antigen-release system and encourages future experimentation with a bovine experimental model.
Fil: Gnazzo, Victoria. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cebrián, José Ignacio. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Langellotti, Cecilia Ana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Chabalgoity, José. Universidad de Concepción del Uruguay; Argentina
Fil: Mongini, Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Quattrocchi, Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina
Fil: Zamorano, Patricia Ines. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description A live system to release heterologous antigens using an attenuated Salmonella strain was developed. We transformed Salmonella typhimurium LVR03 (S. LVR03) with a recombinant pTECH2 vector encoding 0, 1, 2, and 4 tandem copies of an imunogenic peptide of bovine herpes virus-1 (BoHV-1) glycoprotein D (gD). The system used yielded peptides fused to the non-toxic C fragment of the tetanus toxin (TetC), which has been shown to have adjuvant properties. Inoculation of BALB/c mice with the transformed Salmonella strains gave rise to a mild self-limited infection, with primary replication of bacteria occurring in Peyer´s patches, even when the bacteria was administered intranasally. Humoral and cellular immune responses directed against the BoHV-1 antigens were evaluated after oral or intranasal administration of the recombinant bacteria. The results showed that the S. LVR03-dimer vaccine induced specific humoral (IgG in serum and IgG(1) and IgA in saliva), and cellular immune responses (lymphoproliferation and lymphokine secretion), against not only the selected peptide and whole gD, but also against BoHV-1, when administered intranasally. This is the first time Salmonella has been used as an expression vector to induce immunity against BoHV-1. This work demonstrates the feasibility of using this antigen-release system and encourages future experimentation with a bovine experimental model.
publishDate 2012
dc.date.none.fl_str_mv 2012-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/269637
Gnazzo, Victoria; Cebrián, José Ignacio; Langellotti, Cecilia Ana; Chabalgoity, José; Mongini, Claudia; et al.; Immunogenicity of a Bovine Herpes Virus I Peptide Expressed in Tandem Copies in Attenuated Salmonella; Mary Ann Liebert; Viral Immunology; 25; 1; 2-2012; 63-72
0882-8245
CONICET Digital
CONICET
url http://hdl.handle.net/11336/269637
identifier_str_mv Gnazzo, Victoria; Cebrián, José Ignacio; Langellotti, Cecilia Ana; Chabalgoity, José; Mongini, Claudia; et al.; Immunogenicity of a Bovine Herpes Virus I Peptide Expressed in Tandem Copies in Attenuated Salmonella; Mary Ann Liebert; Viral Immunology; 25; 1; 2-2012; 63-72
0882-8245
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.liebertpub.com/doi/10.1089/vim.2011.0031
info:eu-repo/semantics/altIdentifier/doi/10.1089/vim.2011.0031
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Mary Ann Liebert
publisher.none.fl_str_mv Mary Ann Liebert
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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