Mast cells partly contribute to allergic enteritis development: Findings in two different mast cell-deficient mice

Autores
Blanco Pérez, Frank; Gonzalez Menendez, Irene; Stassen, Michael; Kato, Yoichiro; Laiño, Jonathan Emiliano; Kirberg, Jörg; Krause, Maren; Martella, Manuela; Shibata, Noriyuki; Quintanilla-Martinez, Leticia; Feyerabend, Thorsten B.; Rodewald, Hans Reimer; Galli, Stephen J.; Vieths, Stefan; Scheurer, Stephan; Toda, Masako
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Allergic enteritis (AE) is a gastrointestinal form of food allergy. The presence of mast cells and granulocytes has been detected in the inflamed tissues in AE. In this study, we aimed to elucidate the role of mast cells in AE development using two mast cell-deficient mouse strains: KIT(W-sh/W-sh) bearing the W-sash (W(sh)) inversion mutation and Cpa3Cre/+, which lack mast cells due to Cre-mediated mast cell eradication, were used in an AE experimental model. The development of clinical symptoms (e.g. drop in body temperature and weight loss) were abolished in both strains, whereas inflammatory levels of AE (e.g. villous atrophy, edema, and granulocyte accumulation) were reduced mainly in KITW-sh/W-sh mice. FACS analysis of the KITW-sh/W-sh intestinal lamina propria, showed a reduction in the eosinophil (CD45+CD11b+SiglecF+cells) and neutrophil (CD45+CD11b+SiglecF−Ly6G+ cells) accumulation. Cpa3Cre/+ mice showed reduced eosinophil (CD45+CD11b+SiglecF+cells) accumulation, but neutrophil (CD45+CD11b+SiglecF−Ly6G+ cells) accumulation was retained at AE sites. The concentrations of CC chemokine ligand 1 (CCL1), a known CC chemokine receptor 8 ligand leading to eosinophil recruitment, was reduced in intestinal homogenates of both mast cell-deficient mouse strains. These results suggest that mast cells play a role in AE development in part by expressing CCL1 and contributing to eosinophil accumulation at AE. This study offers implications for establishing AE treatments that target infiltrating leucocytes in AE tissues.
Fil: Blanco Pérez, Frank. No especifíca;
Fil: Gonzalez Menendez, Irene. No especifíca;
Fil: Stassen, Michael. Johannes Gutenberg Universitat Mainz; Alemania
Fil: Kato, Yoichiro. Tokyo Women's Medical University; Japón
Fil: Laiño, Jonathan Emiliano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Kirberg, Jörg. No especifíca;
Fil: Krause, Maren. No especifíca;
Fil: Martella, Manuela. No especifíca;
Fil: Shibata, Noriyuki. Tokyo Women's Medical University; Japón
Fil: Quintanilla-Martinez, Leticia. No especifíca;
Fil: Feyerabend, Thorsten B.. No especifíca;
Fil: Rodewald, Hans Reimer. No especifíca;
Fil: Galli, Stephen J.. University of Stanford; Estados Unidos
Fil: Vieths, Stefan. No especifíca;
Fil: Scheurer, Stephan. No especifíca;
Fil: Toda, Masako. No especifíca;
Materia
ALLERGIC ENTERITIS
EOSINOPHIL
FOOD ALLERGY
MAST CELLS
MOUSE MODEL
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/153712
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/153712
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Mast cells partly contribute to allergic enteritis development: Findings in two different mast cell-deficient miceBlanco Pérez, FrankGonzalez Menendez, IreneStassen, MichaelKato, YoichiroLaiño, Jonathan EmilianoKirberg, JörgKrause, MarenMartella, ManuelaShibata, NoriyukiQuintanilla-Martinez, LeticiaFeyerabend, Thorsten B.Rodewald, Hans ReimerGalli, Stephen J.Vieths, StefanScheurer, StephanToda, MasakoALLERGIC ENTERITISEOSINOPHILFOOD ALLERGYMAST CELLSMOUSE MODELhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Allergic enteritis (AE) is a gastrointestinal form of food allergy. The presence of mast cells and granulocytes has been detected in the inflamed tissues in AE. In this study, we aimed to elucidate the role of mast cells in AE development using two mast cell-deficient mouse strains: KIT(W-sh/W-sh) bearing the W-sash (W(sh)) inversion mutation and Cpa3Cre/+, which lack mast cells due to Cre-mediated mast cell eradication, were used in an AE experimental model. The development of clinical symptoms (e.g. drop in body temperature and weight loss) were abolished in both strains, whereas inflammatory levels of AE (e.g. villous atrophy, edema, and granulocyte accumulation) were reduced mainly in KITW-sh/W-sh mice. FACS analysis of the KITW-sh/W-sh intestinal lamina propria, showed a reduction in the eosinophil (CD45+CD11b+SiglecF+cells) and neutrophil (CD45+CD11b+SiglecF−Ly6G+ cells) accumulation. Cpa3Cre/+ mice showed reduced eosinophil (CD45+CD11b+SiglecF+cells) accumulation, but neutrophil (CD45+CD11b+SiglecF−Ly6G+ cells) accumulation was retained at AE sites. The concentrations of CC chemokine ligand 1 (CCL1), a known CC chemokine receptor 8 ligand leading to eosinophil recruitment, was reduced in intestinal homogenates of both mast cell-deficient mouse strains. These results suggest that mast cells play a role in AE development in part by expressing CCL1 and contributing to eosinophil accumulation at AE. This study offers implications for establishing AE treatments that target infiltrating leucocytes in AE tissues.Fil: Blanco Pérez, Frank. No especifíca;Fil: Gonzalez Menendez, Irene. No especifíca;Fil: Stassen, Michael. Johannes Gutenberg Universitat Mainz; AlemaniaFil: Kato, Yoichiro. Tokyo Women's Medical University; JapónFil: Laiño, Jonathan Emiliano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Kirberg, Jörg. No especifíca;Fil: Krause, Maren. No especifíca;Fil: Martella, Manuela. No especifíca;Fil: Shibata, Noriyuki. Tokyo Women's Medical University; JapónFil: Quintanilla-Martinez, Leticia. No especifíca;Fil: Feyerabend, Thorsten B.. No especifíca;Fil: Rodewald, Hans Reimer. No especifíca;Fil: Galli, Stephen J.. University of Stanford; Estados UnidosFil: Vieths, Stefan. No especifíca;Fil: Scheurer, Stephan. No especifíca;Fil: Toda, Masako. No especifíca;Wiley Blackwell Publishing, Inc2021-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/153712Blanco Pérez, Frank; Gonzalez Menendez, Irene; Stassen, Michael; Kato, Yoichiro; Laiño, Jonathan Emiliano; et al.; Mast cells partly contribute to allergic enteritis development: Findings in two different mast cell-deficient mice; Wiley Blackwell Publishing, Inc; Allergy; 77; 3; 11-2021; 1051-10540105-45381398-9995CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1111/all.15182info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1111/all.15182info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:11:59Zoai:ri.conicet.gov.ar:11336/153712instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:12:00.13CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Mast cells partly contribute to allergic enteritis development: Findings in two different mast cell-deficient mice
title Mast cells partly contribute to allergic enteritis development: Findings in two different mast cell-deficient mice
spellingShingle Mast cells partly contribute to allergic enteritis development: Findings in two different mast cell-deficient mice
Blanco Pérez, Frank
ALLERGIC ENTERITIS
EOSINOPHIL
FOOD ALLERGY
MAST CELLS
MOUSE MODEL
title_short Mast cells partly contribute to allergic enteritis development: Findings in two different mast cell-deficient mice
title_full Mast cells partly contribute to allergic enteritis development: Findings in two different mast cell-deficient mice
title_fullStr Mast cells partly contribute to allergic enteritis development: Findings in two different mast cell-deficient mice
title_full_unstemmed Mast cells partly contribute to allergic enteritis development: Findings in two different mast cell-deficient mice
title_sort Mast cells partly contribute to allergic enteritis development: Findings in two different mast cell-deficient mice
dc.creator.none.fl_str_mv Blanco Pérez, Frank
Gonzalez Menendez, Irene
Stassen, Michael
Kato, Yoichiro
Laiño, Jonathan Emiliano
Kirberg, Jörg
Krause, Maren
Martella, Manuela
Shibata, Noriyuki
Quintanilla-Martinez, Leticia
Feyerabend, Thorsten B.
Rodewald, Hans Reimer
Galli, Stephen J.
Vieths, Stefan
Scheurer, Stephan
Toda, Masako
author Blanco Pérez, Frank
author_facet Blanco Pérez, Frank
Gonzalez Menendez, Irene
Stassen, Michael
Kato, Yoichiro
Laiño, Jonathan Emiliano
Kirberg, Jörg
Krause, Maren
Martella, Manuela
Shibata, Noriyuki
Quintanilla-Martinez, Leticia
Feyerabend, Thorsten B.
Rodewald, Hans Reimer
Galli, Stephen J.
Vieths, Stefan
Scheurer, Stephan
Toda, Masako
author_role author
author2 Gonzalez Menendez, Irene
Stassen, Michael
Kato, Yoichiro
Laiño, Jonathan Emiliano
Kirberg, Jörg
Krause, Maren
Martella, Manuela
Shibata, Noriyuki
Quintanilla-Martinez, Leticia
Feyerabend, Thorsten B.
Rodewald, Hans Reimer
Galli, Stephen J.
Vieths, Stefan
Scheurer, Stephan
Toda, Masako
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ALLERGIC ENTERITIS
EOSINOPHIL
FOOD ALLERGY
MAST CELLS
MOUSE MODEL
topic ALLERGIC ENTERITIS
EOSINOPHIL
FOOD ALLERGY
MAST CELLS
MOUSE MODEL
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Allergic enteritis (AE) is a gastrointestinal form of food allergy. The presence of mast cells and granulocytes has been detected in the inflamed tissues in AE. In this study, we aimed to elucidate the role of mast cells in AE development using two mast cell-deficient mouse strains: KIT(W-sh/W-sh) bearing the W-sash (W(sh)) inversion mutation and Cpa3Cre/+, which lack mast cells due to Cre-mediated mast cell eradication, were used in an AE experimental model. The development of clinical symptoms (e.g. drop in body temperature and weight loss) were abolished in both strains, whereas inflammatory levels of AE (e.g. villous atrophy, edema, and granulocyte accumulation) were reduced mainly in KITW-sh/W-sh mice. FACS analysis of the KITW-sh/W-sh intestinal lamina propria, showed a reduction in the eosinophil (CD45+CD11b+SiglecF+cells) and neutrophil (CD45+CD11b+SiglecF−Ly6G+ cells) accumulation. Cpa3Cre/+ mice showed reduced eosinophil (CD45+CD11b+SiglecF+cells) accumulation, but neutrophil (CD45+CD11b+SiglecF−Ly6G+ cells) accumulation was retained at AE sites. The concentrations of CC chemokine ligand 1 (CCL1), a known CC chemokine receptor 8 ligand leading to eosinophil recruitment, was reduced in intestinal homogenates of both mast cell-deficient mouse strains. These results suggest that mast cells play a role in AE development in part by expressing CCL1 and contributing to eosinophil accumulation at AE. This study offers implications for establishing AE treatments that target infiltrating leucocytes in AE tissues.
Fil: Blanco Pérez, Frank. No especifíca;
Fil: Gonzalez Menendez, Irene. No especifíca;
Fil: Stassen, Michael. Johannes Gutenberg Universitat Mainz; Alemania
Fil: Kato, Yoichiro. Tokyo Women's Medical University; Japón
Fil: Laiño, Jonathan Emiliano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Kirberg, Jörg. No especifíca;
Fil: Krause, Maren. No especifíca;
Fil: Martella, Manuela. No especifíca;
Fil: Shibata, Noriyuki. Tokyo Women's Medical University; Japón
Fil: Quintanilla-Martinez, Leticia. No especifíca;
Fil: Feyerabend, Thorsten B.. No especifíca;
Fil: Rodewald, Hans Reimer. No especifíca;
Fil: Galli, Stephen J.. University of Stanford; Estados Unidos
Fil: Vieths, Stefan. No especifíca;
Fil: Scheurer, Stephan. No especifíca;
Fil: Toda, Masako. No especifíca;
description Allergic enteritis (AE) is a gastrointestinal form of food allergy. The presence of mast cells and granulocytes has been detected in the inflamed tissues in AE. In this study, we aimed to elucidate the role of mast cells in AE development using two mast cell-deficient mouse strains: KIT(W-sh/W-sh) bearing the W-sash (W(sh)) inversion mutation and Cpa3Cre/+, which lack mast cells due to Cre-mediated mast cell eradication, were used in an AE experimental model. The development of clinical symptoms (e.g. drop in body temperature and weight loss) were abolished in both strains, whereas inflammatory levels of AE (e.g. villous atrophy, edema, and granulocyte accumulation) were reduced mainly in KITW-sh/W-sh mice. FACS analysis of the KITW-sh/W-sh intestinal lamina propria, showed a reduction in the eosinophil (CD45+CD11b+SiglecF+cells) and neutrophil (CD45+CD11b+SiglecF−Ly6G+ cells) accumulation. Cpa3Cre/+ mice showed reduced eosinophil (CD45+CD11b+SiglecF+cells) accumulation, but neutrophil (CD45+CD11b+SiglecF−Ly6G+ cells) accumulation was retained at AE sites. The concentrations of CC chemokine ligand 1 (CCL1), a known CC chemokine receptor 8 ligand leading to eosinophil recruitment, was reduced in intestinal homogenates of both mast cell-deficient mouse strains. These results suggest that mast cells play a role in AE development in part by expressing CCL1 and contributing to eosinophil accumulation at AE. This study offers implications for establishing AE treatments that target infiltrating leucocytes in AE tissues.
publishDate 2021
dc.date.none.fl_str_mv 2021-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/153712
Blanco Pérez, Frank; Gonzalez Menendez, Irene; Stassen, Michael; Kato, Yoichiro; Laiño, Jonathan Emiliano; et al.; Mast cells partly contribute to allergic enteritis development: Findings in two different mast cell-deficient mice; Wiley Blackwell Publishing, Inc; Allergy; 77; 3; 11-2021; 1051-1054
0105-4538
1398-9995
CONICET Digital
CONICET
url http://hdl.handle.net/11336/153712
identifier_str_mv Blanco Pérez, Frank; Gonzalez Menendez, Irene; Stassen, Michael; Kato, Yoichiro; Laiño, Jonathan Emiliano; et al.; Mast cells partly contribute to allergic enteritis development: Findings in two different mast cell-deficient mice; Wiley Blackwell Publishing, Inc; Allergy; 77; 3; 11-2021; 1051-1054
0105-4538
1398-9995
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1111/all.15182
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1111/all.15182
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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