Design and characterization of soluble biopolymer complexes produced by electrostatic self-assembly of a whey protein isolates and soldium alginate

Autores
Fioramonti, Silvana Alejandra; Perez, Adrián Alejandro; Aríngoli, E. Elena; Rubiolo, Amelia Catalina; Santiago, Liliana Gabriela
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The aim of this study was to design and characterize soluble biopolymer complexes formed by self-assembly of an anionic polysaccharide (sodium alginate, SA) and a whey protein isolate (WPI). First, conditions for producing stable protein aggregates were studied by analysing the effect of concentration (2, 4, 6% w/w) and heating temperature (55, 70, 85 °C) on WPI structural characteristics, using spectroscopic techniques UV–Vis and fluorescence. As heat treatment of WPI at the highest temperature induced the formation of soluble protein aggregates with a greater exposure of hydrophobic patches on their surface, this condition was selected to form protein particles. Secondly, from aqueous solutions of 6% w/w heat-treated WPI at 85 °C, WPI/SA systems were obtained at different ratios (2:1, 4:1 and 6:1) and transmittance measurements as a function of pH (6.0–3.0) were made. Dynamic behaviour of biopolymers in WPI/SA systems was discussed in terms of transition pHs, pHc and pHϕ, corresponding to the formation of WPI-SA soluble complexes and the beginning of associative phase separation (coacervation), respectively. Finally, with these pH values, phase diagrams were constructed for each system. From these diagrams, pH 4.0 was selected to perform deposition of SA onto WPI aggregates surface, as at this pH value formation of WPI-SA soluble complexes was observed for all WPI:SA ratios. Resultant self-assembled biopolymer complexes were then characterized by the same set of spectroscopic techniques. Higher WPI:SA ratios produced soluble self-assembled biopolymer particles where hydrophobic patches of protein aggregates would be more occluded ensuring the protection of potential lipophilic bioactive agents attached inside.
Fil: Fioramonti, Silvana Alejandra. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Tecnología de los Alimentos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Fil: Perez, Adrián Alejandro. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Tecnología de los Alimentos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Fil: Aríngoli, E. Elena. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Tecnología de los Alimentos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Fil: Rubiolo, Amelia Catalina. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Tecnología de los Alimentos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Fil: Santiago, Liliana Gabriela. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Tecnología de los Alimentos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Materia
Whey Protein Isolate
Alginate
Biopolymer Complexes
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/31207
Acceder
id CONICETDig_118ead5e4dc958bef736f2eee8da5c77
oai_identifier_str oai:ri.conicet.gov.ar:11336/31207
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Design and characterization of soluble biopolymer complexes produced by electrostatic self-assembly of a whey protein isolates and soldium alginateFioramonti, Silvana AlejandraPerez, Adrián AlejandroAríngoli, E. ElenaRubiolo, Amelia CatalinaSantiago, Liliana GabrielaWhey Protein IsolateAlginateBiopolymer Complexeshttps://purl.org/becyt/ford/4.4https://purl.org/becyt/ford/4The aim of this study was to design and characterize soluble biopolymer complexes formed by self-assembly of an anionic polysaccharide (sodium alginate, SA) and a whey protein isolate (WPI). First, conditions for producing stable protein aggregates were studied by analysing the effect of concentration (2, 4, 6% w/w) and heating temperature (55, 70, 85 °C) on WPI structural characteristics, using spectroscopic techniques UV–Vis and fluorescence. As heat treatment of WPI at the highest temperature induced the formation of soluble protein aggregates with a greater exposure of hydrophobic patches on their surface, this condition was selected to form protein particles. Secondly, from aqueous solutions of 6% w/w heat-treated WPI at 85 °C, WPI/SA systems were obtained at different ratios (2:1, 4:1 and 6:1) and transmittance measurements as a function of pH (6.0–3.0) were made. Dynamic behaviour of biopolymers in WPI/SA systems was discussed in terms of transition pHs, pHc and pHϕ, corresponding to the formation of WPI-SA soluble complexes and the beginning of associative phase separation (coacervation), respectively. Finally, with these pH values, phase diagrams were constructed for each system. From these diagrams, pH 4.0 was selected to perform deposition of SA onto WPI aggregates surface, as at this pH value formation of WPI-SA soluble complexes was observed for all WPI:SA ratios. Resultant self-assembled biopolymer complexes were then characterized by the same set of spectroscopic techniques. Higher WPI:SA ratios produced soluble self-assembled biopolymer particles where hydrophobic patches of protein aggregates would be more occluded ensuring the protection of potential lipophilic bioactive agents attached inside.Fil: Fioramonti, Silvana Alejandra. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Tecnología de los Alimentos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; ArgentinaFil: Perez, Adrián Alejandro. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Tecnología de los Alimentos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; ArgentinaFil: Aríngoli, E. Elena. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Tecnología de los Alimentos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; ArgentinaFil: Rubiolo, Amelia Catalina. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Tecnología de los Alimentos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; ArgentinaFil: Santiago, Liliana Gabriela. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Tecnología de los Alimentos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; ArgentinaElsevier2013-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/31207Santiago, Liliana Gabriela; Rubiolo, Amelia Catalina; Aríngoli, E. Elena; Perez, Adrián Alejandro; Fioramonti, Silvana Alejandra; Design and characterization of soluble biopolymer complexes produced by electrostatic self-assembly of a whey protein isolates and soldium alginate; Elsevier; Food Hydrocolloids; 35; 5-2013; 129-1360268-005XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0268005X13001367info:eu-repo/semantics/altIdentifier/doi/10.1016/j.foodhyd.2013.05.001info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:08:42Zoai:ri.conicet.gov.ar:11336/31207instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:08:43.125CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Design and characterization of soluble biopolymer complexes produced by electrostatic self-assembly of a whey protein isolates and soldium alginate
title Design and characterization of soluble biopolymer complexes produced by electrostatic self-assembly of a whey protein isolates and soldium alginate
spellingShingle Design and characterization of soluble biopolymer complexes produced by electrostatic self-assembly of a whey protein isolates and soldium alginate
Fioramonti, Silvana Alejandra
Whey Protein Isolate
Alginate
Biopolymer Complexes
title_short Design and characterization of soluble biopolymer complexes produced by electrostatic self-assembly of a whey protein isolates and soldium alginate
title_full Design and characterization of soluble biopolymer complexes produced by electrostatic self-assembly of a whey protein isolates and soldium alginate
title_fullStr Design and characterization of soluble biopolymer complexes produced by electrostatic self-assembly of a whey protein isolates and soldium alginate
title_full_unstemmed Design and characterization of soluble biopolymer complexes produced by electrostatic self-assembly of a whey protein isolates and soldium alginate
title_sort Design and characterization of soluble biopolymer complexes produced by electrostatic self-assembly of a whey protein isolates and soldium alginate
dc.creator.none.fl_str_mv Fioramonti, Silvana Alejandra
Perez, Adrián Alejandro
Aríngoli, E. Elena
Rubiolo, Amelia Catalina
Santiago, Liliana Gabriela
author Fioramonti, Silvana Alejandra
author_facet Fioramonti, Silvana Alejandra
Perez, Adrián Alejandro
Aríngoli, E. Elena
Rubiolo, Amelia Catalina
Santiago, Liliana Gabriela
author_role author
author2 Perez, Adrián Alejandro
Aríngoli, E. Elena
Rubiolo, Amelia Catalina
Santiago, Liliana Gabriela
author2_role author
author
author
author
dc.subject.none.fl_str_mv Whey Protein Isolate
Alginate
Biopolymer Complexes
topic Whey Protein Isolate
Alginate
Biopolymer Complexes
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.4
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv The aim of this study was to design and characterize soluble biopolymer complexes formed by self-assembly of an anionic polysaccharide (sodium alginate, SA) and a whey protein isolate (WPI). First, conditions for producing stable protein aggregates were studied by analysing the effect of concentration (2, 4, 6% w/w) and heating temperature (55, 70, 85 °C) on WPI structural characteristics, using spectroscopic techniques UV–Vis and fluorescence. As heat treatment of WPI at the highest temperature induced the formation of soluble protein aggregates with a greater exposure of hydrophobic patches on their surface, this condition was selected to form protein particles. Secondly, from aqueous solutions of 6% w/w heat-treated WPI at 85 °C, WPI/SA systems were obtained at different ratios (2:1, 4:1 and 6:1) and transmittance measurements as a function of pH (6.0–3.0) were made. Dynamic behaviour of biopolymers in WPI/SA systems was discussed in terms of transition pHs, pHc and pHϕ, corresponding to the formation of WPI-SA soluble complexes and the beginning of associative phase separation (coacervation), respectively. Finally, with these pH values, phase diagrams were constructed for each system. From these diagrams, pH 4.0 was selected to perform deposition of SA onto WPI aggregates surface, as at this pH value formation of WPI-SA soluble complexes was observed for all WPI:SA ratios. Resultant self-assembled biopolymer complexes were then characterized by the same set of spectroscopic techniques. Higher WPI:SA ratios produced soluble self-assembled biopolymer particles where hydrophobic patches of protein aggregates would be more occluded ensuring the protection of potential lipophilic bioactive agents attached inside.
Fil: Fioramonti, Silvana Alejandra. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Tecnología de los Alimentos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Fil: Perez, Adrián Alejandro. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Tecnología de los Alimentos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Fil: Aríngoli, E. Elena. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Tecnología de los Alimentos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Fil: Rubiolo, Amelia Catalina. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Tecnología de los Alimentos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Fil: Santiago, Liliana Gabriela. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Tecnología de los Alimentos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
description The aim of this study was to design and characterize soluble biopolymer complexes formed by self-assembly of an anionic polysaccharide (sodium alginate, SA) and a whey protein isolate (WPI). First, conditions for producing stable protein aggregates were studied by analysing the effect of concentration (2, 4, 6% w/w) and heating temperature (55, 70, 85 °C) on WPI structural characteristics, using spectroscopic techniques UV–Vis and fluorescence. As heat treatment of WPI at the highest temperature induced the formation of soluble protein aggregates with a greater exposure of hydrophobic patches on their surface, this condition was selected to form protein particles. Secondly, from aqueous solutions of 6% w/w heat-treated WPI at 85 °C, WPI/SA systems were obtained at different ratios (2:1, 4:1 and 6:1) and transmittance measurements as a function of pH (6.0–3.0) were made. Dynamic behaviour of biopolymers in WPI/SA systems was discussed in terms of transition pHs, pHc and pHϕ, corresponding to the formation of WPI-SA soluble complexes and the beginning of associative phase separation (coacervation), respectively. Finally, with these pH values, phase diagrams were constructed for each system. From these diagrams, pH 4.0 was selected to perform deposition of SA onto WPI aggregates surface, as at this pH value formation of WPI-SA soluble complexes was observed for all WPI:SA ratios. Resultant self-assembled biopolymer complexes were then characterized by the same set of spectroscopic techniques. Higher WPI:SA ratios produced soluble self-assembled biopolymer particles where hydrophobic patches of protein aggregates would be more occluded ensuring the protection of potential lipophilic bioactive agents attached inside.
publishDate 2013
dc.date.none.fl_str_mv 2013-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/31207
Santiago, Liliana Gabriela; Rubiolo, Amelia Catalina; Aríngoli, E. Elena; Perez, Adrián Alejandro; Fioramonti, Silvana Alejandra; Design and characterization of soluble biopolymer complexes produced by electrostatic self-assembly of a whey protein isolates and soldium alginate; Elsevier; Food Hydrocolloids; 35; 5-2013; 129-136
0268-005X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/31207
identifier_str_mv Santiago, Liliana Gabriela; Rubiolo, Amelia Catalina; Aríngoli, E. Elena; Perez, Adrián Alejandro; Fioramonti, Silvana Alejandra; Design and characterization of soluble biopolymer complexes produced by electrostatic self-assembly of a whey protein isolates and soldium alginate; Elsevier; Food Hydrocolloids; 35; 5-2013; 129-136
0268-005X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0268005X13001367
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.foodhyd.2013.05.001
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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