Adjuvant effects of Lactobacillus casei added to a renutrition diet in a malnourished mouse model

Autores
Gauffin Cano, María Paola; Agüero, Graciela; Perdigon, Gabriela del Valle
Año de publicación
2002
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Nutritional deficiencies are associated with impaired immune response, affecting the body’s defence mechanisms. It is also known that Lactic Acid Bacteria (LAB) and fermented products such us yogurt have immunopotentiator activity and nutritional properties, and could thus be used as a valuable supplement in a renutrition diet. The aim of this study was to determine, in a non-severe malnutrition model, the effective dose of Lactobacillus casei (L.casei), which when is used as an adjuvant in a renutrition diet, would modulate the mucosal immune system and induce recovery of the integrity of the intestinal barrier. The experiments were performed on groups of malnourished and renourished BALB/c mice. They received after milk renutrition a supplement of different doses and periods of L. casei feeding. We measured body weight; hematologic values and serum proteins. We also characterized small intestine immunoglobulin secreting cells, intraepithelial leukocytes, mastocytes and goblet cells. Structural and ultrastructural studies were performed. Our results suggest that impaired gut barrier and mucosal immune function produced by malnutrition can be reversed by L. casei and that the dose of 107 cfu/day/mouse administered during 5 consecutive days was the optimal one for recovery of the gut mucosal immune system. The clinical significance of these findings suggests ways for improving mucosal immunity, and generating protection against enteropathogens in hosts immunosuppressed by malnutrition.
Fil: Gauffin Cano, María Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Agüero, Graciela. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Bioquímica Aplicada; Argentina
Fil: Perdigon, Gabriela del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Materia
Malnutrition
Mucosal Immunity
Lactobacillus Casei
Gut Barrier
Renutrition Diet
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/58640
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/58640
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Adjuvant effects of Lactobacillus casei added to a renutrition diet in a malnourished mouse modelGauffin Cano, María PaolaAgüero, GracielaPerdigon, Gabriela del ValleMalnutritionMucosal ImmunityLactobacillus CaseiGut BarrierRenutrition Diethttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Nutritional deficiencies are associated with impaired immune response, affecting the body’s defence mechanisms. It is also known that Lactic Acid Bacteria (LAB) and fermented products such us yogurt have immunopotentiator activity and nutritional properties, and could thus be used as a valuable supplement in a renutrition diet. The aim of this study was to determine, in a non-severe malnutrition model, the effective dose of Lactobacillus casei (L.casei), which when is used as an adjuvant in a renutrition diet, would modulate the mucosal immune system and induce recovery of the integrity of the intestinal barrier. The experiments were performed on groups of malnourished and renourished BALB/c mice. They received after milk renutrition a supplement of different doses and periods of L. casei feeding. We measured body weight; hematologic values and serum proteins. We also characterized small intestine immunoglobulin secreting cells, intraepithelial leukocytes, mastocytes and goblet cells. Structural and ultrastructural studies were performed. Our results suggest that impaired gut barrier and mucosal immune function produced by malnutrition can be reversed by L. casei and that the dose of 107 cfu/day/mouse administered during 5 consecutive days was the optimal one for recovery of the gut mucosal immune system. The clinical significance of these findings suggests ways for improving mucosal immunity, and generating protection against enteropathogens in hosts immunosuppressed by malnutrition.Fil: Gauffin Cano, María Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Agüero, Graciela. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Bioquímica Aplicada; ArgentinaFil: Perdigon, Gabriela del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaSociedad Latinoamericana de Microscopía Electrónica2002-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/58640Gauffin Cano, María Paola; Agüero, Graciela; Perdigon, Gabriela del Valle; Adjuvant effects of Lactobacillus casei added to a renutrition diet in a malnourished mouse model; Sociedad Latinoamericana de Microscopía Electrónica; Biocell; 26; 1; 4-2002; 35-480327-95451667-5746CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mendoza-conicet.gob.ar/portal/biocell/vol/pdf/26/04.pdfinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:30:28Zoai:ri.conicet.gov.ar:11336/58640instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:30:28.987CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Adjuvant effects of Lactobacillus casei added to a renutrition diet in a malnourished mouse model
title Adjuvant effects of Lactobacillus casei added to a renutrition diet in a malnourished mouse model
spellingShingle Adjuvant effects of Lactobacillus casei added to a renutrition diet in a malnourished mouse model
Gauffin Cano, María Paola
Malnutrition
Mucosal Immunity
Lactobacillus Casei
Gut Barrier
Renutrition Diet
title_short Adjuvant effects of Lactobacillus casei added to a renutrition diet in a malnourished mouse model
title_full Adjuvant effects of Lactobacillus casei added to a renutrition diet in a malnourished mouse model
title_fullStr Adjuvant effects of Lactobacillus casei added to a renutrition diet in a malnourished mouse model
title_full_unstemmed Adjuvant effects of Lactobacillus casei added to a renutrition diet in a malnourished mouse model
title_sort Adjuvant effects of Lactobacillus casei added to a renutrition diet in a malnourished mouse model
dc.creator.none.fl_str_mv Gauffin Cano, María Paola
Agüero, Graciela
Perdigon, Gabriela del Valle
author Gauffin Cano, María Paola
author_facet Gauffin Cano, María Paola
Agüero, Graciela
Perdigon, Gabriela del Valle
author_role author
author2 Agüero, Graciela
Perdigon, Gabriela del Valle
author2_role author
author
dc.subject.none.fl_str_mv Malnutrition
Mucosal Immunity
Lactobacillus Casei
Gut Barrier
Renutrition Diet
topic Malnutrition
Mucosal Immunity
Lactobacillus Casei
Gut Barrier
Renutrition Diet
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Nutritional deficiencies are associated with impaired immune response, affecting the body’s defence mechanisms. It is also known that Lactic Acid Bacteria (LAB) and fermented products such us yogurt have immunopotentiator activity and nutritional properties, and could thus be used as a valuable supplement in a renutrition diet. The aim of this study was to determine, in a non-severe malnutrition model, the effective dose of Lactobacillus casei (L.casei), which when is used as an adjuvant in a renutrition diet, would modulate the mucosal immune system and induce recovery of the integrity of the intestinal barrier. The experiments were performed on groups of malnourished and renourished BALB/c mice. They received after milk renutrition a supplement of different doses and periods of L. casei feeding. We measured body weight; hematologic values and serum proteins. We also characterized small intestine immunoglobulin secreting cells, intraepithelial leukocytes, mastocytes and goblet cells. Structural and ultrastructural studies were performed. Our results suggest that impaired gut barrier and mucosal immune function produced by malnutrition can be reversed by L. casei and that the dose of 107 cfu/day/mouse administered during 5 consecutive days was the optimal one for recovery of the gut mucosal immune system. The clinical significance of these findings suggests ways for improving mucosal immunity, and generating protection against enteropathogens in hosts immunosuppressed by malnutrition.
Fil: Gauffin Cano, María Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Agüero, Graciela. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Bioquímica Aplicada; Argentina
Fil: Perdigon, Gabriela del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
description Nutritional deficiencies are associated with impaired immune response, affecting the body’s defence mechanisms. It is also known that Lactic Acid Bacteria (LAB) and fermented products such us yogurt have immunopotentiator activity and nutritional properties, and could thus be used as a valuable supplement in a renutrition diet. The aim of this study was to determine, in a non-severe malnutrition model, the effective dose of Lactobacillus casei (L.casei), which when is used as an adjuvant in a renutrition diet, would modulate the mucosal immune system and induce recovery of the integrity of the intestinal barrier. The experiments were performed on groups of malnourished and renourished BALB/c mice. They received after milk renutrition a supplement of different doses and periods of L. casei feeding. We measured body weight; hematologic values and serum proteins. We also characterized small intestine immunoglobulin secreting cells, intraepithelial leukocytes, mastocytes and goblet cells. Structural and ultrastructural studies were performed. Our results suggest that impaired gut barrier and mucosal immune function produced by malnutrition can be reversed by L. casei and that the dose of 107 cfu/day/mouse administered during 5 consecutive days was the optimal one for recovery of the gut mucosal immune system. The clinical significance of these findings suggests ways for improving mucosal immunity, and generating protection against enteropathogens in hosts immunosuppressed by malnutrition.
publishDate 2002
dc.date.none.fl_str_mv 2002-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/58640
Gauffin Cano, María Paola; Agüero, Graciela; Perdigon, Gabriela del Valle; Adjuvant effects of Lactobacillus casei added to a renutrition diet in a malnourished mouse model; Sociedad Latinoamericana de Microscopía Electrónica; Biocell; 26; 1; 4-2002; 35-48
0327-9545
1667-5746
CONICET Digital
CONICET
url http://hdl.handle.net/11336/58640
identifier_str_mv Gauffin Cano, María Paola; Agüero, Graciela; Perdigon, Gabriela del Valle; Adjuvant effects of Lactobacillus casei added to a renutrition diet in a malnourished mouse model; Sociedad Latinoamericana de Microscopía Electrónica; Biocell; 26; 1; 4-2002; 35-48
0327-9545
1667-5746
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mendoza-conicet.gob.ar/portal/biocell/vol/pdf/26/04.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Sociedad Latinoamericana de Microscopía Electrónica
publisher.none.fl_str_mv Sociedad Latinoamericana de Microscopía Electrónica
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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