Nasal administration of Lactobacillus rhamnosus CRL 1505 improves humoral immunity in malnourished mice

Autores
Barbieri, N.; Herrera, Héctor Matías; Salva, Maria Susana; Villena, Julio Cesar; Álvarez, S.
Año de publicación
2010
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
We evaluated the effect of Lactobacillus rhamnosus CRL1505 (Lr05) nasal treatment on the recovery of humoral immune response in malnourished immunocompromised mice. Weaning mice were malnourished with protein free diet for 21d. Malnourished mice were fed a balanced conventional diet (BD) for 7 days or BD for 7 days with Lr05 nasal treatment on days 6 and 7 (BD+Lr05). On day 8 we studied B lymphocytes population in blood, spleen and bone marrow. Expression of B220 and HSA were determined by flow cytometry. Total lymphocytes counts and B220+HSA+ cells in BD+Lr05 mice were significantly higher than those in the BD group. To assess whether changes induced by BD+Lr05 treatment modifies the resistance to infection and the humoral immune response; we performed an intranasal challenge with Streptococcus pneumoniae (Sp). BD mice showed lung and blood positive cultures throughout the studied period. Treatment with BD+Lr05 increased resistance to infection, prevented the dissemination of Sp into blood and improved lung clearance. Levels of specific IgG in the serum of BD+Lr05 mice were higher than in the BD group. In addition, mice treated with BD+Lr05 showed higher levels of specific respiratory IgA. Nasal administration of Lr05 improved humoral immunity and increased resistance against Sp infection. Nasal treatments with lactic acid bacteria would be an interesting alternative to improve defenses in immunocompromised hosts.
Fil: Barbieri, N.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Herrera, Héctor Matías. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Tucumán; Argentina
Fil: Salva, Maria Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Álvarez, S.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán; Argentina
XXVI Jornadas Científicas de la Asociación d Biología de Tucumán
Tafi del Valle
Argentina
Asociación de Biología de Tucumán
Materia
MALNUTRITION
LACTOBACILLUS RHAMNOSUS
HUMORAL IMMUNITY RECOVERY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/262620
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Nasal administration of Lactobacillus rhamnosus CRL 1505 improves humoral immunity in malnourished miceBarbieri, N.Herrera, Héctor MatíasSalva, Maria SusanaVillena, Julio CesarÁlvarez, S.MALNUTRITIONLACTOBACILLUS RHAMNOSUSHUMORAL IMMUNITY RECOVERYhttps://purl.org/becyt/ford/3.4https://purl.org/becyt/ford/3We evaluated the effect of Lactobacillus rhamnosus CRL1505 (Lr05) nasal treatment on the recovery of humoral immune response in malnourished immunocompromised mice. Weaning mice were malnourished with protein free diet for 21d. Malnourished mice were fed a balanced conventional diet (BD) for 7 days or BD for 7 days with Lr05 nasal treatment on days 6 and 7 (BD+Lr05). On day 8 we studied B lymphocytes population in blood, spleen and bone marrow. Expression of B220 and HSA were determined by flow cytometry. Total lymphocytes counts and B220+HSA+ cells in BD+Lr05 mice were significantly higher than those in the BD group. To assess whether changes induced by BD+Lr05 treatment modifies the resistance to infection and the humoral immune response; we performed an intranasal challenge with Streptococcus pneumoniae (Sp). BD mice showed lung and blood positive cultures throughout the studied period. Treatment with BD+Lr05 increased resistance to infection, prevented the dissemination of Sp into blood and improved lung clearance. Levels of specific IgG in the serum of BD+Lr05 mice were higher than in the BD group. In addition, mice treated with BD+Lr05 showed higher levels of specific respiratory IgA. Nasal administration of Lr05 improved humoral immunity and increased resistance against Sp infection. Nasal treatments with lactic acid bacteria would be an interesting alternative to improve defenses in immunocompromised hosts.Fil: Barbieri, N.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Herrera, Héctor Matías. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Tucumán; ArgentinaFil: Salva, Maria Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Álvarez, S.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán; ArgentinaXXVI Jornadas Científicas de la Asociación d Biología de TucumánTafi del ValleArgentinaAsociación de Biología de TucumánTech Science Press2010info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectJornadaJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/262620Nasal administration of Lactobacillus rhamnosus CRL 1505 improves humoral immunity in malnourished mice; XXVI Jornadas Científicas de la Asociación d Biología de Tucumán; Tafi del Valle; Argentina; 2009; 151-1510327-9545CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://asobioltuc.com/listado.php?id=2Nacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:02:14Zoai:ri.conicet.gov.ar:11336/262620instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:02:15.137CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Nasal administration of Lactobacillus rhamnosus CRL 1505 improves humoral immunity in malnourished mice
title Nasal administration of Lactobacillus rhamnosus CRL 1505 improves humoral immunity in malnourished mice
spellingShingle Nasal administration of Lactobacillus rhamnosus CRL 1505 improves humoral immunity in malnourished mice
Barbieri, N.
MALNUTRITION
LACTOBACILLUS RHAMNOSUS
HUMORAL IMMUNITY RECOVERY
title_short Nasal administration of Lactobacillus rhamnosus CRL 1505 improves humoral immunity in malnourished mice
title_full Nasal administration of Lactobacillus rhamnosus CRL 1505 improves humoral immunity in malnourished mice
title_fullStr Nasal administration of Lactobacillus rhamnosus CRL 1505 improves humoral immunity in malnourished mice
title_full_unstemmed Nasal administration of Lactobacillus rhamnosus CRL 1505 improves humoral immunity in malnourished mice
title_sort Nasal administration of Lactobacillus rhamnosus CRL 1505 improves humoral immunity in malnourished mice
dc.creator.none.fl_str_mv Barbieri, N.
Herrera, Héctor Matías
Salva, Maria Susana
Villena, Julio Cesar
Álvarez, S.
author Barbieri, N.
author_facet Barbieri, N.
Herrera, Héctor Matías
Salva, Maria Susana
Villena, Julio Cesar
Álvarez, S.
author_role author
author2 Herrera, Héctor Matías
Salva, Maria Susana
Villena, Julio Cesar
Álvarez, S.
author2_role author
author
author
author
dc.subject.none.fl_str_mv MALNUTRITION
LACTOBACILLUS RHAMNOSUS
HUMORAL IMMUNITY RECOVERY
topic MALNUTRITION
LACTOBACILLUS RHAMNOSUS
HUMORAL IMMUNITY RECOVERY
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.4
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv We evaluated the effect of Lactobacillus rhamnosus CRL1505 (Lr05) nasal treatment on the recovery of humoral immune response in malnourished immunocompromised mice. Weaning mice were malnourished with protein free diet for 21d. Malnourished mice were fed a balanced conventional diet (BD) for 7 days or BD for 7 days with Lr05 nasal treatment on days 6 and 7 (BD+Lr05). On day 8 we studied B lymphocytes population in blood, spleen and bone marrow. Expression of B220 and HSA were determined by flow cytometry. Total lymphocytes counts and B220+HSA+ cells in BD+Lr05 mice were significantly higher than those in the BD group. To assess whether changes induced by BD+Lr05 treatment modifies the resistance to infection and the humoral immune response; we performed an intranasal challenge with Streptococcus pneumoniae (Sp). BD mice showed lung and blood positive cultures throughout the studied period. Treatment with BD+Lr05 increased resistance to infection, prevented the dissemination of Sp into blood and improved lung clearance. Levels of specific IgG in the serum of BD+Lr05 mice were higher than in the BD group. In addition, mice treated with BD+Lr05 showed higher levels of specific respiratory IgA. Nasal administration of Lr05 improved humoral immunity and increased resistance against Sp infection. Nasal treatments with lactic acid bacteria would be an interesting alternative to improve defenses in immunocompromised hosts.
Fil: Barbieri, N.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Herrera, Héctor Matías. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Tucumán; Argentina
Fil: Salva, Maria Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Álvarez, S.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán; Argentina
XXVI Jornadas Científicas de la Asociación d Biología de Tucumán
Tafi del Valle
Argentina
Asociación de Biología de Tucumán
description We evaluated the effect of Lactobacillus rhamnosus CRL1505 (Lr05) nasal treatment on the recovery of humoral immune response in malnourished immunocompromised mice. Weaning mice were malnourished with protein free diet for 21d. Malnourished mice were fed a balanced conventional diet (BD) for 7 days or BD for 7 days with Lr05 nasal treatment on days 6 and 7 (BD+Lr05). On day 8 we studied B lymphocytes population in blood, spleen and bone marrow. Expression of B220 and HSA were determined by flow cytometry. Total lymphocytes counts and B220+HSA+ cells in BD+Lr05 mice were significantly higher than those in the BD group. To assess whether changes induced by BD+Lr05 treatment modifies the resistance to infection and the humoral immune response; we performed an intranasal challenge with Streptococcus pneumoniae (Sp). BD mice showed lung and blood positive cultures throughout the studied period. Treatment with BD+Lr05 increased resistance to infection, prevented the dissemination of Sp into blood and improved lung clearance. Levels of specific IgG in the serum of BD+Lr05 mice were higher than in the BD group. In addition, mice treated with BD+Lr05 showed higher levels of specific respiratory IgA. Nasal administration of Lr05 improved humoral immunity and increased resistance against Sp infection. Nasal treatments with lactic acid bacteria would be an interesting alternative to improve defenses in immunocompromised hosts.
publishDate 2010
dc.date.none.fl_str_mv 2010
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Jornada
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/262620
Nasal administration of Lactobacillus rhamnosus CRL 1505 improves humoral immunity in malnourished mice; XXVI Jornadas Científicas de la Asociación d Biología de Tucumán; Tafi del Valle; Argentina; 2009; 151-151
0327-9545
CONICET Digital
CONICET
url http://hdl.handle.net/11336/262620
identifier_str_mv Nasal administration of Lactobacillus rhamnosus CRL 1505 improves humoral immunity in malnourished mice; XXVI Jornadas Científicas de la Asociación d Biología de Tucumán; Tafi del Valle; Argentina; 2009; 151-151
0327-9545
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
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dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Tech Science Press
publisher.none.fl_str_mv Tech Science Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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