Regulatory B cells phenotype in patients with chronic Chagas Disease upon T. cruzi stimulation

Autores
Girard, Magalí Celeste; Acevedo, Gonzalo Raúl; Beati, Maria Paula; Fernandez, Marisa; Hernandez, Yolanda; Chadi, Raul; Gomez, Karina Andrea
Año de publicación
2019
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Chronic Chagas disease (CCD) caused by T. cruzi infection is associated with an imbalance between an excessive inflammatory reaction and a defective immunomodulatory profile caused by host-parasite interaction. Despite the growing importance of regulatory B cells in many malignancies, the role of these population remains elusive in CCD. Here, we used flow cytometry to evaluate the phenotype of peripheral blood B cells, isolated from CCD patients with or without cardiac involvement, according to their expression of CD19, CD24, CD38 and CD27. Cells were incubated with or without T. cruzi lysate, and/or CpG and CD40L, and IL-10 production was also assessed by intracellular cytokine staining (ICS). In order to overcome the absence of subset-specific hallmark membrane markers, we performed an automated and nonsupervised analysis method called flowPeaks, based on k-means algorithm for data clustering. flowPeaks identified five clusters according to the simultaneous assessment of the fluorescent markers mentioned above. We found that in T. cruzi-stimulated conditions, CD19highCD27lowCD24low B cells were diminished in CCD patients compared to non-infected donors (p<0.05), while CD19highCD27highCD24high B cells were augmented only in patients with cardiac compromise (p<0.05). Both B cells clusters express variable amounts of CD38. In addition, the proportion of IL-10-producing B cells (B10 cells) was increased in T. cruzi stimulated samples in CCD patients and non-infected donors (p<0.05). Our results suggest that cardiac CCD patients display a memory B cell phenotype upon exposure to T. cruzi, although IL10 production by B cells may be consequence of an innate response triggered by the parasite.
Fil: Girard, Magalí Celeste. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Acevedo, Gonzalo Raúl. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Beati, Maria Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Fernandez, Marisa. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”. Instituto Nacional de Parasitología "Dr. Mario Fatala Chaben”; Argentina
Fil: Hernandez, Yolanda. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”. Instituto Nacional de Parasitología "Dr. Mario Fatala Chaben”; Argentina
Fil: Chadi, Raul. Gobierno de la Ciudad Autónoma de Buenos Aires. Hospital General de Agudos Doctor Ignacio Pirovano; Argentina
Fil: Gomez, Karina Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
LXVII Reunión Científica Anual de Sociedad Argentina de Inmunología
San Miguel de Tucumán
Argentina
Sociedad Argentina de Inmunologia
Materia
TRYPANOSOMA CRUZI
B CELLS
REGULATORY B CELLS
CHAGAS DISEASE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/275562
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Regulatory B cells phenotype in patients with chronic Chagas Disease upon T. cruzi stimulationGirard, Magalí CelesteAcevedo, Gonzalo RaúlBeati, Maria PaulaFernandez, MarisaHernandez, YolandaChadi, RaulGomez, Karina AndreaTRYPANOSOMA CRUZIB CELLSREGULATORY B CELLSCHAGAS DISEASEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Chronic Chagas disease (CCD) caused by T. cruzi infection is associated with an imbalance between an excessive inflammatory reaction and a defective immunomodulatory profile caused by host-parasite interaction. Despite the growing importance of regulatory B cells in many malignancies, the role of these population remains elusive in CCD. Here, we used flow cytometry to evaluate the phenotype of peripheral blood B cells, isolated from CCD patients with or without cardiac involvement, according to their expression of CD19, CD24, CD38 and CD27. Cells were incubated with or without T. cruzi lysate, and/or CpG and CD40L, and IL-10 production was also assessed by intracellular cytokine staining (ICS). In order to overcome the absence of subset-specific hallmark membrane markers, we performed an automated and nonsupervised analysis method called flowPeaks, based on k-means algorithm for data clustering. flowPeaks identified five clusters according to the simultaneous assessment of the fluorescent markers mentioned above. We found that in T. cruzi-stimulated conditions, CD19highCD27lowCD24low B cells were diminished in CCD patients compared to non-infected donors (p<0.05), while CD19highCD27highCD24high B cells were augmented only in patients with cardiac compromise (p<0.05). Both B cells clusters express variable amounts of CD38. In addition, the proportion of IL-10-producing B cells (B10 cells) was increased in T. cruzi stimulated samples in CCD patients and non-infected donors (p<0.05). Our results suggest that cardiac CCD patients display a memory B cell phenotype upon exposure to T. cruzi, although IL10 production by B cells may be consequence of an innate response triggered by the parasite.Fil: Girard, Magalí Celeste. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Acevedo, Gonzalo Raúl. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Beati, Maria Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Fernandez, Marisa. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”. Instituto Nacional de Parasitología "Dr. Mario Fatala Chaben”; ArgentinaFil: Hernandez, Yolanda. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”. Instituto Nacional de Parasitología "Dr. Mario Fatala Chaben”; ArgentinaFil: Chadi, Raul. Gobierno de la Ciudad Autónoma de Buenos Aires. Hospital General de Agudos Doctor Ignacio Pirovano; ArgentinaFil: Gomez, Karina Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaLXVII Reunión Científica Anual de Sociedad Argentina de InmunologíaSan Miguel de TucumánArgentinaSociedad Argentina de InmunologiaSociedad Argentina de Inmunología2019info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/275562Regulatory B cells phenotype in patients with chronic Chagas Disease upon T. cruzi stimulation; LXVII Reunión Científica Anual de Sociedad Argentina de Inmunología; San Miguel de Tucumán; Argentina; 2019; 63-63978-987-47408-0-9CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://inmunologia.org.ar/lvii-reunion-anual-de-la-sociedad-argentina-de-inmunologia/Nacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-12-03T08:56:59Zoai:ri.conicet.gov.ar:11336/275562instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-12-03 08:57:00.087CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Regulatory B cells phenotype in patients with chronic Chagas Disease upon T. cruzi stimulation
title Regulatory B cells phenotype in patients with chronic Chagas Disease upon T. cruzi stimulation
spellingShingle Regulatory B cells phenotype in patients with chronic Chagas Disease upon T. cruzi stimulation
Girard, Magalí Celeste
TRYPANOSOMA CRUZI
B CELLS
REGULATORY B CELLS
CHAGAS DISEASE
title_short Regulatory B cells phenotype in patients with chronic Chagas Disease upon T. cruzi stimulation
title_full Regulatory B cells phenotype in patients with chronic Chagas Disease upon T. cruzi stimulation
title_fullStr Regulatory B cells phenotype in patients with chronic Chagas Disease upon T. cruzi stimulation
title_full_unstemmed Regulatory B cells phenotype in patients with chronic Chagas Disease upon T. cruzi stimulation
title_sort Regulatory B cells phenotype in patients with chronic Chagas Disease upon T. cruzi stimulation
dc.creator.none.fl_str_mv Girard, Magalí Celeste
Acevedo, Gonzalo Raúl
Beati, Maria Paula
Fernandez, Marisa
Hernandez, Yolanda
Chadi, Raul
Gomez, Karina Andrea
author Girard, Magalí Celeste
author_facet Girard, Magalí Celeste
Acevedo, Gonzalo Raúl
Beati, Maria Paula
Fernandez, Marisa
Hernandez, Yolanda
Chadi, Raul
Gomez, Karina Andrea
author_role author
author2 Acevedo, Gonzalo Raúl
Beati, Maria Paula
Fernandez, Marisa
Hernandez, Yolanda
Chadi, Raul
Gomez, Karina Andrea
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv TRYPANOSOMA CRUZI
B CELLS
REGULATORY B CELLS
CHAGAS DISEASE
topic TRYPANOSOMA CRUZI
B CELLS
REGULATORY B CELLS
CHAGAS DISEASE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Chronic Chagas disease (CCD) caused by T. cruzi infection is associated with an imbalance between an excessive inflammatory reaction and a defective immunomodulatory profile caused by host-parasite interaction. Despite the growing importance of regulatory B cells in many malignancies, the role of these population remains elusive in CCD. Here, we used flow cytometry to evaluate the phenotype of peripheral blood B cells, isolated from CCD patients with or without cardiac involvement, according to their expression of CD19, CD24, CD38 and CD27. Cells were incubated with or without T. cruzi lysate, and/or CpG and CD40L, and IL-10 production was also assessed by intracellular cytokine staining (ICS). In order to overcome the absence of subset-specific hallmark membrane markers, we performed an automated and nonsupervised analysis method called flowPeaks, based on k-means algorithm for data clustering. flowPeaks identified five clusters according to the simultaneous assessment of the fluorescent markers mentioned above. We found that in T. cruzi-stimulated conditions, CD19highCD27lowCD24low B cells were diminished in CCD patients compared to non-infected donors (p<0.05), while CD19highCD27highCD24high B cells were augmented only in patients with cardiac compromise (p<0.05). Both B cells clusters express variable amounts of CD38. In addition, the proportion of IL-10-producing B cells (B10 cells) was increased in T. cruzi stimulated samples in CCD patients and non-infected donors (p<0.05). Our results suggest that cardiac CCD patients display a memory B cell phenotype upon exposure to T. cruzi, although IL10 production by B cells may be consequence of an innate response triggered by the parasite.
Fil: Girard, Magalí Celeste. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Acevedo, Gonzalo Raúl. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Beati, Maria Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Fernandez, Marisa. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”. Instituto Nacional de Parasitología "Dr. Mario Fatala Chaben”; Argentina
Fil: Hernandez, Yolanda. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”. Instituto Nacional de Parasitología "Dr. Mario Fatala Chaben”; Argentina
Fil: Chadi, Raul. Gobierno de la Ciudad Autónoma de Buenos Aires. Hospital General de Agudos Doctor Ignacio Pirovano; Argentina
Fil: Gomez, Karina Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
LXVII Reunión Científica Anual de Sociedad Argentina de Inmunología
San Miguel de Tucumán
Argentina
Sociedad Argentina de Inmunologia
description Chronic Chagas disease (CCD) caused by T. cruzi infection is associated with an imbalance between an excessive inflammatory reaction and a defective immunomodulatory profile caused by host-parasite interaction. Despite the growing importance of regulatory B cells in many malignancies, the role of these population remains elusive in CCD. Here, we used flow cytometry to evaluate the phenotype of peripheral blood B cells, isolated from CCD patients with or without cardiac involvement, according to their expression of CD19, CD24, CD38 and CD27. Cells were incubated with or without T. cruzi lysate, and/or CpG and CD40L, and IL-10 production was also assessed by intracellular cytokine staining (ICS). In order to overcome the absence of subset-specific hallmark membrane markers, we performed an automated and nonsupervised analysis method called flowPeaks, based on k-means algorithm for data clustering. flowPeaks identified five clusters according to the simultaneous assessment of the fluorescent markers mentioned above. We found that in T. cruzi-stimulated conditions, CD19highCD27lowCD24low B cells were diminished in CCD patients compared to non-infected donors (p<0.05), while CD19highCD27highCD24high B cells were augmented only in patients with cardiac compromise (p<0.05). Both B cells clusters express variable amounts of CD38. In addition, the proportion of IL-10-producing B cells (B10 cells) was increased in T. cruzi stimulated samples in CCD patients and non-infected donors (p<0.05). Our results suggest that cardiac CCD patients display a memory B cell phenotype upon exposure to T. cruzi, although IL10 production by B cells may be consequence of an innate response triggered by the parasite.
publishDate 2019
dc.date.none.fl_str_mv 2019
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/275562
Regulatory B cells phenotype in patients with chronic Chagas Disease upon T. cruzi stimulation; LXVII Reunión Científica Anual de Sociedad Argentina de Inmunología; San Miguel de Tucumán; Argentina; 2019; 63-63
978-987-47408-0-9
CONICET Digital
CONICET
url http://hdl.handle.net/11336/275562
identifier_str_mv Regulatory B cells phenotype in patients with chronic Chagas Disease upon T. cruzi stimulation; LXVII Reunión Científica Anual de Sociedad Argentina de Inmunología; San Miguel de Tucumán; Argentina; 2019; 63-63
978-987-47408-0-9
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
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dc.format.none.fl_str_mv application/pdf
application/pdf
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dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Sociedad Argentina de Inmunología
publisher.none.fl_str_mv Sociedad Argentina de Inmunología
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
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repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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