Altered frequency of CD24highCD38high transitional B cells in patients with cardiac involvement of chronic Chagas disease.

Autores
Girard, Magalí Celeste; Acevedo, Gonzalo Raúl; Ossowski, Micaela Soledad; Alcaraz, Paula Belén; Fernández, Marisa; Hernandez, Yolanda; Chadi, Raul; Gomez, Karina Andrea
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The cardiomyopathy developed by patients with chronic Chagas disease (CCD), one ofthe most severe consequences of T. cruzi infection, is mainly associated with animbalance between an excessive inflammatory reaction and a defectiveimmunomodulatory profile cause by host-parasite interaction. Despite the growingimportance of the regulatory function of B-cells in many malignancies, few studies haveaddressed their immunosuppressive role in chronic Chagas disease. In this work, wetackled this issue by studying the proportion of different B cell subpopulations and theircapacity to secrete IL-10 in individuals with distinct clinical forms of CCD. Seven-colourflow cytometry was performed to examine the peripheral blood B cell compartment inchronic Chagas disease (CCD) patients with and without cardiac manifestations (n=10 foreach group) and non-infected donors (n=9). Peripheral blood mononuclear cells (PBMC)were incubated for 5h with PMA, ionomicyn and brefeldin A. According to the expressionof markers CD19, CD24 and CD38, we showed an expansion of total B cell andtransitional CD24highCD38high B cell subsets in CCD patients with cardiac involvementcompared to non-infected donors. Furthermore, although no differences were observed inthe frequency of total IL-10 producing B cells (B10) among the groups, CCD patients withcardiac involvement showed a statistically significant increased proportion of naïve B10cells and a tendency to an increased frequency of transitional B10 cells compared to noninfected donors. These findings suggest that immature transitional CD24highCD38high Bcells are greatly expanded in patients with the cardiac form of chronic Chagas disease andthese cells retain their ability to secrete IL-10 compared to non-infected donors.Furthermore, the distribution of naïve, transitional and memory B cells inside the B10 cellsfollowed the same pattern in chronic patients without cardiac involvement and non-infectedindividuals. Our work provides insight into the phenotypic distribution of regulatory B cell inCCD, an important step towards new strategies to prevent cardiomiopathy associated withT. cruzi infection
Fil: Girard, Magalí Celeste. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Acevedo, Gonzalo Raúl. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Ossowski, Micaela Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Alcaraz, Paula Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Fernández, Marisa. Instituto Nacional de Diagnostico E Investigacion de la Enfermedad de Chagas Dr. Mario Fatala Chaben; Argentina
Fil: Hernandez, Yolanda. Instituto Nacional de Diagnostico E Investigacion de la Enfermedad de Chagas Dr. Mario Fatala Chaben; Argentina
Fil: Chadi, Raul. Hospital General de Agudos Dr. Ignacio Pirovano; Argentina
Fil: Gomez, Karina Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Materia
REGULATORY B CELLS
CHRONIC CHAGAS DISEASE
TRYPANOSOMA CRUZI
HUMAN B10 CELLS
TRANSITIONAL B CELLS
PROTOZOAN PARASITE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/230579
Acceder
id CONICETDig_f840308b1284dc3d00eb27703619ae7b
oai_identifier_str oai:ri.conicet.gov.ar:11336/230579
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Altered frequency of CD24highCD38high transitional B cells in patients with cardiac involvement of chronic Chagas disease.Girard, Magalí CelesteAcevedo, Gonzalo RaúlOssowski, Micaela SoledadAlcaraz, Paula BelénFernández, MarisaHernandez, YolandaChadi, RaulGomez, Karina AndreaREGULATORY B CELLSCHRONIC CHAGAS DISEASETRYPANOSOMA CRUZIHUMAN B10 CELLSTRANSITIONAL B CELLSPROTOZOAN PARASITEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The cardiomyopathy developed by patients with chronic Chagas disease (CCD), one ofthe most severe consequences of T. cruzi infection, is mainly associated with animbalance between an excessive inflammatory reaction and a defectiveimmunomodulatory profile cause by host-parasite interaction. Despite the growingimportance of the regulatory function of B-cells in many malignancies, few studies haveaddressed their immunosuppressive role in chronic Chagas disease. In this work, wetackled this issue by studying the proportion of different B cell subpopulations and theircapacity to secrete IL-10 in individuals with distinct clinical forms of CCD. Seven-colourflow cytometry was performed to examine the peripheral blood B cell compartment inchronic Chagas disease (CCD) patients with and without cardiac manifestations (n=10 foreach group) and non-infected donors (n=9). Peripheral blood mononuclear cells (PBMC)were incubated for 5h with PMA, ionomicyn and brefeldin A. According to the expressionof markers CD19, CD24 and CD38, we showed an expansion of total B cell andtransitional CD24highCD38high B cell subsets in CCD patients with cardiac involvementcompared to non-infected donors. Furthermore, although no differences were observed inthe frequency of total IL-10 producing B cells (B10) among the groups, CCD patients withcardiac involvement showed a statistically significant increased proportion of naïve B10cells and a tendency to an increased frequency of transitional B10 cells compared to noninfected donors. These findings suggest that immature transitional CD24highCD38high Bcells are greatly expanded in patients with the cardiac form of chronic Chagas disease andthese cells retain their ability to secrete IL-10 compared to non-infected donors.Furthermore, the distribution of naïve, transitional and memory B cells inside the B10 cellsfollowed the same pattern in chronic patients without cardiac involvement and non-infectedindividuals. Our work provides insight into the phenotypic distribution of regulatory B cell inCCD, an important step towards new strategies to prevent cardiomiopathy associated withT. cruzi infectionFil: Girard, Magalí Celeste. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Acevedo, Gonzalo Raúl. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Ossowski, Micaela Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Alcaraz, Paula Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Fernández, Marisa. Instituto Nacional de Diagnostico E Investigacion de la Enfermedad de Chagas Dr. Mario Fatala Chaben; ArgentinaFil: Hernandez, Yolanda. Instituto Nacional de Diagnostico E Investigacion de la Enfermedad de Chagas Dr. Mario Fatala Chaben; ArgentinaFil: Chadi, Raul. Hospital General de Agudos Dr. Ignacio Pirovano; ArgentinaFil: Gomez, Karina Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaCold Spring Harbor Laboratory Press2019-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/230579Girard, Magalí Celeste; Acevedo, Gonzalo Raúl; Ossowski, Micaela Soledad; Alcaraz, Paula Belén; Fernández, Marisa; et al.; Altered frequency of CD24highCD38high transitional B cells in patients with cardiac involvement of chronic Chagas disease.; Cold Spring Harbor Laboratory Press; bioRxiv; 7-2019; 1-232692-8205CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.biorxiv.org/content/10.1101/684589v2.full.pdfinfo:eu-repo/semantics/altIdentifier/doi/10.1101/684589info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:19:44Zoai:ri.conicet.gov.ar:11336/230579instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:19:45.06CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Altered frequency of CD24highCD38high transitional B cells in patients with cardiac involvement of chronic Chagas disease.
title Altered frequency of CD24highCD38high transitional B cells in patients with cardiac involvement of chronic Chagas disease.
spellingShingle Altered frequency of CD24highCD38high transitional B cells in patients with cardiac involvement of chronic Chagas disease.
Girard, Magalí Celeste
REGULATORY B CELLS
CHRONIC CHAGAS DISEASE
TRYPANOSOMA CRUZI
HUMAN B10 CELLS
TRANSITIONAL B CELLS
PROTOZOAN PARASITE
title_short Altered frequency of CD24highCD38high transitional B cells in patients with cardiac involvement of chronic Chagas disease.
title_full Altered frequency of CD24highCD38high transitional B cells in patients with cardiac involvement of chronic Chagas disease.
title_fullStr Altered frequency of CD24highCD38high transitional B cells in patients with cardiac involvement of chronic Chagas disease.
title_full_unstemmed Altered frequency of CD24highCD38high transitional B cells in patients with cardiac involvement of chronic Chagas disease.
title_sort Altered frequency of CD24highCD38high transitional B cells in patients with cardiac involvement of chronic Chagas disease.
dc.creator.none.fl_str_mv Girard, Magalí Celeste
Acevedo, Gonzalo Raúl
Ossowski, Micaela Soledad
Alcaraz, Paula Belén
Fernández, Marisa
Hernandez, Yolanda
Chadi, Raul
Gomez, Karina Andrea
author Girard, Magalí Celeste
author_facet Girard, Magalí Celeste
Acevedo, Gonzalo Raúl
Ossowski, Micaela Soledad
Alcaraz, Paula Belén
Fernández, Marisa
Hernandez, Yolanda
Chadi, Raul
Gomez, Karina Andrea
author_role author
author2 Acevedo, Gonzalo Raúl
Ossowski, Micaela Soledad
Alcaraz, Paula Belén
Fernández, Marisa
Hernandez, Yolanda
Chadi, Raul
Gomez, Karina Andrea
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv REGULATORY B CELLS
CHRONIC CHAGAS DISEASE
TRYPANOSOMA CRUZI
HUMAN B10 CELLS
TRANSITIONAL B CELLS
PROTOZOAN PARASITE
topic REGULATORY B CELLS
CHRONIC CHAGAS DISEASE
TRYPANOSOMA CRUZI
HUMAN B10 CELLS
TRANSITIONAL B CELLS
PROTOZOAN PARASITE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The cardiomyopathy developed by patients with chronic Chagas disease (CCD), one ofthe most severe consequences of T. cruzi infection, is mainly associated with animbalance between an excessive inflammatory reaction and a defectiveimmunomodulatory profile cause by host-parasite interaction. Despite the growingimportance of the regulatory function of B-cells in many malignancies, few studies haveaddressed their immunosuppressive role in chronic Chagas disease. In this work, wetackled this issue by studying the proportion of different B cell subpopulations and theircapacity to secrete IL-10 in individuals with distinct clinical forms of CCD. Seven-colourflow cytometry was performed to examine the peripheral blood B cell compartment inchronic Chagas disease (CCD) patients with and without cardiac manifestations (n=10 foreach group) and non-infected donors (n=9). Peripheral blood mononuclear cells (PBMC)were incubated for 5h with PMA, ionomicyn and brefeldin A. According to the expressionof markers CD19, CD24 and CD38, we showed an expansion of total B cell andtransitional CD24highCD38high B cell subsets in CCD patients with cardiac involvementcompared to non-infected donors. Furthermore, although no differences were observed inthe frequency of total IL-10 producing B cells (B10) among the groups, CCD patients withcardiac involvement showed a statistically significant increased proportion of naïve B10cells and a tendency to an increased frequency of transitional B10 cells compared to noninfected donors. These findings suggest that immature transitional CD24highCD38high Bcells are greatly expanded in patients with the cardiac form of chronic Chagas disease andthese cells retain their ability to secrete IL-10 compared to non-infected donors.Furthermore, the distribution of naïve, transitional and memory B cells inside the B10 cellsfollowed the same pattern in chronic patients without cardiac involvement and non-infectedindividuals. Our work provides insight into the phenotypic distribution of regulatory B cell inCCD, an important step towards new strategies to prevent cardiomiopathy associated withT. cruzi infection
Fil: Girard, Magalí Celeste. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Acevedo, Gonzalo Raúl. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Ossowski, Micaela Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Alcaraz, Paula Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Fernández, Marisa. Instituto Nacional de Diagnostico E Investigacion de la Enfermedad de Chagas Dr. Mario Fatala Chaben; Argentina
Fil: Hernandez, Yolanda. Instituto Nacional de Diagnostico E Investigacion de la Enfermedad de Chagas Dr. Mario Fatala Chaben; Argentina
Fil: Chadi, Raul. Hospital General de Agudos Dr. Ignacio Pirovano; Argentina
Fil: Gomez, Karina Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
description The cardiomyopathy developed by patients with chronic Chagas disease (CCD), one ofthe most severe consequences of T. cruzi infection, is mainly associated with animbalance between an excessive inflammatory reaction and a defectiveimmunomodulatory profile cause by host-parasite interaction. Despite the growingimportance of the regulatory function of B-cells in many malignancies, few studies haveaddressed their immunosuppressive role in chronic Chagas disease. In this work, wetackled this issue by studying the proportion of different B cell subpopulations and theircapacity to secrete IL-10 in individuals with distinct clinical forms of CCD. Seven-colourflow cytometry was performed to examine the peripheral blood B cell compartment inchronic Chagas disease (CCD) patients with and without cardiac manifestations (n=10 foreach group) and non-infected donors (n=9). Peripheral blood mononuclear cells (PBMC)were incubated for 5h with PMA, ionomicyn and brefeldin A. According to the expressionof markers CD19, CD24 and CD38, we showed an expansion of total B cell andtransitional CD24highCD38high B cell subsets in CCD patients with cardiac involvementcompared to non-infected donors. Furthermore, although no differences were observed inthe frequency of total IL-10 producing B cells (B10) among the groups, CCD patients withcardiac involvement showed a statistically significant increased proportion of naïve B10cells and a tendency to an increased frequency of transitional B10 cells compared to noninfected donors. These findings suggest that immature transitional CD24highCD38high Bcells are greatly expanded in patients with the cardiac form of chronic Chagas disease andthese cells retain their ability to secrete IL-10 compared to non-infected donors.Furthermore, the distribution of naïve, transitional and memory B cells inside the B10 cellsfollowed the same pattern in chronic patients without cardiac involvement and non-infectedindividuals. Our work provides insight into the phenotypic distribution of regulatory B cell inCCD, an important step towards new strategies to prevent cardiomiopathy associated withT. cruzi infection
publishDate 2019
dc.date.none.fl_str_mv 2019-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/230579
Girard, Magalí Celeste; Acevedo, Gonzalo Raúl; Ossowski, Micaela Soledad; Alcaraz, Paula Belén; Fernández, Marisa; et al.; Altered frequency of CD24highCD38high transitional B cells in patients with cardiac involvement of chronic Chagas disease.; Cold Spring Harbor Laboratory Press; bioRxiv; 7-2019; 1-23
2692-8205
CONICET Digital
CONICET
url http://hdl.handle.net/11336/230579
identifier_str_mv Girard, Magalí Celeste; Acevedo, Gonzalo Raúl; Ossowski, Micaela Soledad; Alcaraz, Paula Belén; Fernández, Marisa; et al.; Altered frequency of CD24highCD38high transitional B cells in patients with cardiac involvement of chronic Chagas disease.; Cold Spring Harbor Laboratory Press; bioRxiv; 7-2019; 1-23
2692-8205
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.biorxiv.org/content/10.1101/684589v2.full.pdf
info:eu-repo/semantics/altIdentifier/doi/10.1101/684589
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Cold Spring Harbor Laboratory Press
publisher.none.fl_str_mv Cold Spring Harbor Laboratory Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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