Learning experiences comprising central ethanol exposure in rat neonates: Effects upon respiratory plasticity and the brain catalase system

Autores
Trujillo, Verónica; Macchione, Ana Fabiola; Albretch, Paula Alejandra; Deza Ponzio, Romina; Virgolini, Miriam Beatriz; Molina, Juan Carlos
Año de publicación
2019
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Fetal ethanol (EtOH) exposure represents a risk factor for the Sudden Infant Death Syndrome and it’s early effects upon respiration also promotes hypoxic ischemic consequences. This study analyzes central ethanol’s effects upon breathing plasticity during a stage in the development of the rat equivalent to the 3rd human gestational trimester. The study not only analyzed ethanol’s unconditioned breathing effects but also how they are regulated by learning processes. Taking into account that ethanol is primarily metabolized in the brain via the catalase system, we examined the effects of early history with the drug upon the activity of this enzymatic system. During postnatal days 3, 5 and 7 (PDs 3-7) pups either received intracisternal (i.c.) administrations of vehicle or ethanol (300 mg%). They were subsequently exposed to a whole body plethysmograph under normoxia. The apparatus was scented or not with the ethanol odor. The presence of the odorant increased breathing rates. The state of intoxication attenuated the onset of apneas; a phenomenon indicative of an antianxiety effect of the drug given the state of arousal caused by the novel environment, maternal deprivation and the stress of i.c. administrations. At PD9, pups were tested while sober under sequential air conditions (initial-normoxia, hypoxia and recovery-normoxia). Once again the plethysmograh was unscented or contained EtOH odor. Prior experience with the scented chamber associated with EtOH’s central effects elicited a conditioned isodirectional response relative to the onset of apneas previously observed during PDs 3-7. Yet, prior history with the drug exacerbated the onset of apneas when pups were defied with hypoxia. Following this test, pups ingested 0.8 g/kg of absolute EtOH and their brains were analyzed to determine catalase activity. Pre-exposure to EtOH’s central effects paired with the odor of the drug resulted in heightened enzymatic activity. The results indicate that central EtOH accumulation may exert antianxiety effects that attenuate apneic disruptions but that also has long-lasting effects upon respiratory plasticity under hypoxia. Most importantly, these effects appear to be related with how the brain catalase system reacts to the presence of EtOH in accordance with the nature of prior experiences with the drug.
Fil: Trujillo, Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina
Fil: Macchione, Ana Fabiola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina. Universidad Nacional de Córdoba. Facultad de Psicología; Argentina
Fil: Albretch, Paula Alejandra. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Farmacología; Argentina
Fil: Deza Ponzio, Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Farmacología Experimental de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Farmacología Experimental de Córdoba; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Farmacología; Argentina
Fil: Virgolini, Miriam Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Farmacología Experimental de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Farmacología Experimental de Córdoba; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Farmacología; Argentina
Fil: Molina, Juan Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina. Universidad Nacional de Córdoba. Facultad de Psicología; Argentina
IX International Meeting of the Latin American Society for Biomedical Research on Alcoholism (LASBRA): Determinants of Alcoholism: Bridging the gap between epidemiological and basic research
Córdoba
Argentina
Latin American Society for Biomedical Research on Alcoholism
Materia
CATALASE
NEONATE
ETHANOL
CONDITIONING
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/168854
Acceder
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spelling Learning experiences comprising central ethanol exposure in rat neonates: Effects upon respiratory plasticity and the brain catalase systemTrujillo, VerónicaMacchione, Ana FabiolaAlbretch, Paula AlejandraDeza Ponzio, RominaVirgolini, Miriam BeatrizMolina, Juan CarlosCATALASENEONATEETHANOLCONDITIONINGhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Fetal ethanol (EtOH) exposure represents a risk factor for the Sudden Infant Death Syndrome and it’s early effects upon respiration also promotes hypoxic ischemic consequences. This study analyzes central ethanol’s effects upon breathing plasticity during a stage in the development of the rat equivalent to the 3rd human gestational trimester. The study not only analyzed ethanol’s unconditioned breathing effects but also how they are regulated by learning processes. Taking into account that ethanol is primarily metabolized in the brain via the catalase system, we examined the effects of early history with the drug upon the activity of this enzymatic system. During postnatal days 3, 5 and 7 (PDs 3-7) pups either received intracisternal (i.c.) administrations of vehicle or ethanol (300 mg%). They were subsequently exposed to a whole body plethysmograph under normoxia. The apparatus was scented or not with the ethanol odor. The presence of the odorant increased breathing rates. The state of intoxication attenuated the onset of apneas; a phenomenon indicative of an antianxiety effect of the drug given the state of arousal caused by the novel environment, maternal deprivation and the stress of i.c. administrations. At PD9, pups were tested while sober under sequential air conditions (initial-normoxia, hypoxia and recovery-normoxia). Once again the plethysmograh was unscented or contained EtOH odor. Prior experience with the scented chamber associated with EtOH’s central effects elicited a conditioned isodirectional response relative to the onset of apneas previously observed during PDs 3-7. Yet, prior history with the drug exacerbated the onset of apneas when pups were defied with hypoxia. Following this test, pups ingested 0.8 g/kg of absolute EtOH and their brains were analyzed to determine catalase activity. Pre-exposure to EtOH’s central effects paired with the odor of the drug resulted in heightened enzymatic activity. The results indicate that central EtOH accumulation may exert antianxiety effects that attenuate apneic disruptions but that also has long-lasting effects upon respiratory plasticity under hypoxia. Most importantly, these effects appear to be related with how the brain catalase system reacts to the presence of EtOH in accordance with the nature of prior experiences with the drug.Fil: Trujillo, Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; ArgentinaFil: Macchione, Ana Fabiola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina. Universidad Nacional de Córdoba. Facultad de Psicología; ArgentinaFil: Albretch, Paula Alejandra. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Farmacología; ArgentinaFil: Deza Ponzio, Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Farmacología Experimental de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Farmacología Experimental de Córdoba; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Farmacología; ArgentinaFil: Virgolini, Miriam Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Farmacología Experimental de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Farmacología Experimental de Córdoba; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Farmacología; ArgentinaFil: Molina, Juan Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina. Universidad Nacional de Córdoba. Facultad de Psicología; ArgentinaIX International Meeting of the Latin American Society for Biomedical Research on Alcoholism (LASBRA): Determinants of Alcoholism: Bridging the gap between epidemiological and basic researchCórdobaArgentinaLatin American Society for Biomedical Research on AlcoholismDougmar Publishing Group2019info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectEncuentroJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/168854Learning experiences comprising central ethanol exposure in rat neonates: Effects upon respiratory plasticity and the brain catalase system; IX International Meeting of the Latin American Society for Biomedical Research on Alcoholism (LASBRA): Determinants of Alcoholism: Bridging the gap between epidemiological and basic research; Córdoba; Argentina; 2019; 51-51CONICET DigitalCONICETenginfo:eu-repo/semantics/reference/url/https://ri.conicet.gov.ar/handle/11336/129486info:eu-repo/semantics/altIdentifier/url/http://jfasrp.com/index.php/JFASRP/article/view/7Internacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:45:56Zoai:ri.conicet.gov.ar:11336/168854instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:45:56.83CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Learning experiences comprising central ethanol exposure in rat neonates: Effects upon respiratory plasticity and the brain catalase system
title Learning experiences comprising central ethanol exposure in rat neonates: Effects upon respiratory plasticity and the brain catalase system
spellingShingle Learning experiences comprising central ethanol exposure in rat neonates: Effects upon respiratory plasticity and the brain catalase system
Trujillo, Verónica
CATALASE
NEONATE
ETHANOL
CONDITIONING
title_short Learning experiences comprising central ethanol exposure in rat neonates: Effects upon respiratory plasticity and the brain catalase system
title_full Learning experiences comprising central ethanol exposure in rat neonates: Effects upon respiratory plasticity and the brain catalase system
title_fullStr Learning experiences comprising central ethanol exposure in rat neonates: Effects upon respiratory plasticity and the brain catalase system
title_full_unstemmed Learning experiences comprising central ethanol exposure in rat neonates: Effects upon respiratory plasticity and the brain catalase system
title_sort Learning experiences comprising central ethanol exposure in rat neonates: Effects upon respiratory plasticity and the brain catalase system
dc.creator.none.fl_str_mv Trujillo, Verónica
Macchione, Ana Fabiola
Albretch, Paula Alejandra
Deza Ponzio, Romina
Virgolini, Miriam Beatriz
Molina, Juan Carlos
author Trujillo, Verónica
author_facet Trujillo, Verónica
Macchione, Ana Fabiola
Albretch, Paula Alejandra
Deza Ponzio, Romina
Virgolini, Miriam Beatriz
Molina, Juan Carlos
author_role author
author2 Macchione, Ana Fabiola
Albretch, Paula Alejandra
Deza Ponzio, Romina
Virgolini, Miriam Beatriz
Molina, Juan Carlos
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv CATALASE
NEONATE
ETHANOL
CONDITIONING
topic CATALASE
NEONATE
ETHANOL
CONDITIONING
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Fetal ethanol (EtOH) exposure represents a risk factor for the Sudden Infant Death Syndrome and it’s early effects upon respiration also promotes hypoxic ischemic consequences. This study analyzes central ethanol’s effects upon breathing plasticity during a stage in the development of the rat equivalent to the 3rd human gestational trimester. The study not only analyzed ethanol’s unconditioned breathing effects but also how they are regulated by learning processes. Taking into account that ethanol is primarily metabolized in the brain via the catalase system, we examined the effects of early history with the drug upon the activity of this enzymatic system. During postnatal days 3, 5 and 7 (PDs 3-7) pups either received intracisternal (i.c.) administrations of vehicle or ethanol (300 mg%). They were subsequently exposed to a whole body plethysmograph under normoxia. The apparatus was scented or not with the ethanol odor. The presence of the odorant increased breathing rates. The state of intoxication attenuated the onset of apneas; a phenomenon indicative of an antianxiety effect of the drug given the state of arousal caused by the novel environment, maternal deprivation and the stress of i.c. administrations. At PD9, pups were tested while sober under sequential air conditions (initial-normoxia, hypoxia and recovery-normoxia). Once again the plethysmograh was unscented or contained EtOH odor. Prior experience with the scented chamber associated with EtOH’s central effects elicited a conditioned isodirectional response relative to the onset of apneas previously observed during PDs 3-7. Yet, prior history with the drug exacerbated the onset of apneas when pups were defied with hypoxia. Following this test, pups ingested 0.8 g/kg of absolute EtOH and their brains were analyzed to determine catalase activity. Pre-exposure to EtOH’s central effects paired with the odor of the drug resulted in heightened enzymatic activity. The results indicate that central EtOH accumulation may exert antianxiety effects that attenuate apneic disruptions but that also has long-lasting effects upon respiratory plasticity under hypoxia. Most importantly, these effects appear to be related with how the brain catalase system reacts to the presence of EtOH in accordance with the nature of prior experiences with the drug.
Fil: Trujillo, Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina
Fil: Macchione, Ana Fabiola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina. Universidad Nacional de Córdoba. Facultad de Psicología; Argentina
Fil: Albretch, Paula Alejandra. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Farmacología; Argentina
Fil: Deza Ponzio, Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Farmacología Experimental de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Farmacología Experimental de Córdoba; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Farmacología; Argentina
Fil: Virgolini, Miriam Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Farmacología Experimental de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Farmacología Experimental de Córdoba; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Farmacología; Argentina
Fil: Molina, Juan Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina. Universidad Nacional de Córdoba. Facultad de Psicología; Argentina
IX International Meeting of the Latin American Society for Biomedical Research on Alcoholism (LASBRA): Determinants of Alcoholism: Bridging the gap between epidemiological and basic research
Córdoba
Argentina
Latin American Society for Biomedical Research on Alcoholism
description Fetal ethanol (EtOH) exposure represents a risk factor for the Sudden Infant Death Syndrome and it’s early effects upon respiration also promotes hypoxic ischemic consequences. This study analyzes central ethanol’s effects upon breathing plasticity during a stage in the development of the rat equivalent to the 3rd human gestational trimester. The study not only analyzed ethanol’s unconditioned breathing effects but also how they are regulated by learning processes. Taking into account that ethanol is primarily metabolized in the brain via the catalase system, we examined the effects of early history with the drug upon the activity of this enzymatic system. During postnatal days 3, 5 and 7 (PDs 3-7) pups either received intracisternal (i.c.) administrations of vehicle or ethanol (300 mg%). They were subsequently exposed to a whole body plethysmograph under normoxia. The apparatus was scented or not with the ethanol odor. The presence of the odorant increased breathing rates. The state of intoxication attenuated the onset of apneas; a phenomenon indicative of an antianxiety effect of the drug given the state of arousal caused by the novel environment, maternal deprivation and the stress of i.c. administrations. At PD9, pups were tested while sober under sequential air conditions (initial-normoxia, hypoxia and recovery-normoxia). Once again the plethysmograh was unscented or contained EtOH odor. Prior experience with the scented chamber associated with EtOH’s central effects elicited a conditioned isodirectional response relative to the onset of apneas previously observed during PDs 3-7. Yet, prior history with the drug exacerbated the onset of apneas when pups were defied with hypoxia. Following this test, pups ingested 0.8 g/kg of absolute EtOH and their brains were analyzed to determine catalase activity. Pre-exposure to EtOH’s central effects paired with the odor of the drug resulted in heightened enzymatic activity. The results indicate that central EtOH accumulation may exert antianxiety effects that attenuate apneic disruptions but that also has long-lasting effects upon respiratory plasticity under hypoxia. Most importantly, these effects appear to be related with how the brain catalase system reacts to the presence of EtOH in accordance with the nature of prior experiences with the drug.
publishDate 2019
dc.date.none.fl_str_mv 2019
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Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
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dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/168854
Learning experiences comprising central ethanol exposure in rat neonates: Effects upon respiratory plasticity and the brain catalase system; IX International Meeting of the Latin American Society for Biomedical Research on Alcoholism (LASBRA): Determinants of Alcoholism: Bridging the gap between epidemiological and basic research; Córdoba; Argentina; 2019; 51-51
CONICET Digital
CONICET
url http://hdl.handle.net/11336/168854
identifier_str_mv Learning experiences comprising central ethanol exposure in rat neonates: Effects upon respiratory plasticity and the brain catalase system; IX International Meeting of the Latin American Society for Biomedical Research on Alcoholism (LASBRA): Determinants of Alcoholism: Bridging the gap between epidemiological and basic research; Córdoba; Argentina; 2019; 51-51
CONICET Digital
CONICET
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publisher.none.fl_str_mv Dougmar Publishing Group
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