Oxygen deficiency and salinity affect cell-specific ion concentrations in adventitious roots of barley (Hordeum vulgare)

• Autores: Kotula, Lukasz; Clode, Peta L.; Striker, Gustavo Gabriel; Pedersen, Ole; Läuchli, Andre; Shabala, Sergey; Colmer, Timothy D.
• Año de publicación: 2015
• Idioma: inglés
• Tipo de recurso: artículo
• Materia: Barley; Oxygen Deficiency; Salinity-Waterlogging Interaction; X-Ray Microanalysis
• Institución: Consejo Nacional de Investigaciones Científicas y Técnicas

Oxygen deficiency associated with soil waterlogging adversely impacts root respiration and nutrient acquisition. We investigated the effects of O2 deficiency and salinity (100 mM NaCl) on radial O2 concentrations and cell-specific ion distributions in adventitious roots of barley (Hordeum vulgare). Microelectrode profiling measured O2 concentrations across roots in aerated, aerated saline, stagnant or stagnant saline media. X-ray microanalysis at two positions behind the apex determined the cell-specific elemental concentrations of potassium (K), sodium (Na) and chloride (Cl) across roots. Severe O2 deficiency occurred in the stele and apical regions of roots in stagnant solutions. O2 deficiency in the stele reduced the concentrations of K, Na and Cl in the pericycle and xylem parenchyma cells at the subapical region. Near the root apex, Na declined across the cortex in roots from the aerated saline solution but was relatively high in all cell types in roots from the stagnant saline solution. Oxygen deficiency has a substantial impact on cellular ion concentrations in roots. Both pericycle and xylem parenchyma cells are involved in energy-dependent K loading into the xylem and in controlling radial Na and Cl transport. At root tips, accumulation of Na in the outer cell layers likely contributed to reduction of Na in inner cells of the tips.
Fil: Kotula, Lukasz. University of Western Australia. Faculty of Science. School of Plant Biology; Australia
Fil: Clode, Peta L.. University of Western Australia. Centre for Microscopy, Characterisation and Analysis; Australia
Fil: Striker, Gustavo Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina. University of Western Australia. Faculty of Science. School of Plant Biology; Australia
Fil: Pedersen, Ole. University of Western Australia. Faculty of Science. School of Plant Biology; Australia. University of Western Australia. Institute of Advanced Studies; Australia. University of Copenhagen. Freshwater Biological Laboratory; Dinamarca
Fil: Läuchli, Andre. University of Western Australia. Faculty of Science. School of Plant Biology; Australia. University of California. Department of Land, Air and Water Resources; Estados Unidos
Fil: Shabala, Sergey. University of Tasmania. School of Land and Food; Australia
Fil: Colmer, Timothy D.. University of Western Australia. Faculty of Science. School of Plant Biology; Australia. University of Western Australia. UWA Institute of Agriculture; Australia